Last updated: 2020-11-29
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Knit directory: OAStrain/
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Unstaged changes:
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These are the previous versions of the repository in which changes were made to the R Markdown (analysis/DE_analysis.Rmd) and HTML (docs/DE_analysis.html) files. If you’ve configured a remote Git repository (see ?wflow_git_remote), click on the hyperlinks in the table below to view the files as they were in that past version.
| File | Version | Author | Date | Message |
|---|---|---|---|---|
| html | f906578 | Anthony Hung | 2020-11-29 | include sample 2 |
| html | 824d084 | Anthony Hung | 2020-11-29 | rerun with all samples included |
| Rmd | c2ccaf4 | Anthony Hung | 2020-11-29 | run analyses without excluding sample 2 |
| html | c2ccaf4 | Anthony Hung | 2020-11-29 | run analyses without excluding sample 2 |
| Rmd | 0e96722 | Anthony Hung | 2020-10-29 | Add fasttopics on external data |
| Rmd | 01e5357 | Anthony Hung | 2020-10-04 | kegg pathway analysis with biogrid |
| Rmd | e9fd8b4 | Anthony Hung | 2020-10-04 | add pathway anlaysis |
| Rmd | 71db882 | Anthony Hung | 2020-10-04 | add pathfindR |
| html | 6d6d1b5 | Anthony Hung | 2020-10-04 | knit permutation analyses of strain labels |
| Rmd | 1a4fcd8 | Anthony Hung | 2020-10-04 | knit permutation analyses of strain labels |
| Rmd | 757b4e7 | Anthony Hung | 2020-10-01 | add fasttopics analysis |
| html | 09ab774 | Anthony Hung | 2020-09-27 | add html files |
| html | e855314 | Anthony Hung | 2020-09-27 | delete html files |
| Rmd | 83148a8 | Anthony Hung | 2020-09-27 | add more PCs to PCA plot |
| Rmd | 71d27f7 | Anthony Hung | 2020-09-22 | pseudobulk limma de, integrate across individuals, add n100 for pwr analysis |
| html | 71d27f7 | Anthony Hung | 2020-09-22 | pseudobulk limma de, integrate across individuals, add n100 for pwr analysis |
| Rmd | 0aa15d4 | Anthony Hung | 2020-09-10 | normalize the topic memberships to add up to 1 for each cell |
| Rmd | e3ee7ee | Anthony Hung | 2020-09-02 | add links to index page |
| Rmd | 32d620f | Anthony Hung | 2020-08-23 | power analysis curves |
| Rmd | 9055627 | Anthony Hung | 2020-06-22 | add up/down counts table |
| html | 9055627 | Anthony Hung | 2020-06-22 | add up/down counts table |
| Rmd | 66f54cc | Anthony Hung | 2020-06-21 | individual volcano plots |
| html | 66f54cc | Anthony Hung | 2020-06-21 | individual volcano plots |
| Rmd | cf0b215 | Anthony Hung | 2020-06-14 | print out all interaction genes in boxplot |
| html | 2cb3b3f | Anthony Hung | 2020-06-01 | add interaction term genes and plotting |
| Rmd | 78b9cc0 | Anthony Hung | 2020-05-31 | add dotplots of 50 overlap genes |
| Rmd | 6cfbae9 | Anthony Hung | 2020-05-18 | Add enrichment analysis file |
| Rmd | a5b1c7c | Anthony Hung | 2020-04-14 | separate into up/downregulated in DE analysis |
| html | a5b1c7c | Anthony Hung | 2020-04-14 | separate into up/downregulated in DE analysis |
| Rmd | e6d3fad | Anthony Hung | 2020-04-05 | Subset pseudobulk to 1000 DEG, dotplots instead of boxplots |
| html | e6d3fad | Anthony Hung | 2020-04-05 | Subset pseudobulk to 1000 DEG, dotplots instead of boxplots |
| Rmd | c0e7c45 | Anthony Hung | 2020-03-23 | Add boxplot by sample for top DEGenes; k=7 for supervised definingh early and late chondros |
| html | c0e7c45 | Anthony Hung | 2020-03-23 | Add boxplot by sample for top DEGenes; k=7 for supervised definingh early and late chondros |
| Rmd | 940b53e | Anthony Hung | 2020-03-23 | define early/late passage chondros as a supervised cluster |
| Rmd | ad1e27b | Anthony Hung | 2020-03-02 | separate up/down in de analysis |
| html | ad1e27b | Anthony Hung | 2020-03-02 | separate up/down in de analysis |
| html | 88682cc | Anthony Hung | 2020-02-19 | add additional chondro markers |
| Rmd | b967c3c | Anthony Hung | 2020-01-27 | Add cell atlas assignment |
| html | b967c3c | Anthony Hung | 2020-01-27 | Add cell atlas assignment |
| Rmd | 797305a | Anthony Hung | 2020-01-18 | include Individual as a FE |
| html | 797305a | Anthony Hung | 2020-01-18 | include Individual as a FE |
| html | a356514 | Anthony Hung | 2020-01-18 | knit analysis files |
| Rmd | 3c163de | Anthony Hung | 2020-01-18 | reorder by pvalue |
| Rmd | cb6a0a5 | Anthony Hung | 2020-01-18 | edgeR DE analysis |
| html | cb6a0a5 | Anthony Hung | 2020-01-18 | edgeR DE analysis |
| Rmd | 158fdf3 | Anthony Hung | 2020-01-15 | Use quant_normalization |
| html | 158fdf3 | Anthony Hung | 2020-01-15 | Use quant_normalization |
| Rmd | 4e7c93c | Anthony Hung | 2020-01-13 | Remove 18856 strain |
| html | 4e7c93c | Anthony Hung | 2020-01-13 | Remove 18856 strain |
| Rmd | 00a66fb | Anthony Hung | 2020-01-13 | run GO enrichment analysis |
| html | 00a66fb | Anthony Hung | 2020-01-13 | run GO enrichment analysis |
| Rmd | a04a1ac | Anthony Hung | 2020-01-13 | Add GO analysis |
| html | a04a1ac | Anthony Hung | 2020-01-13 | Add GO analysis |
| Rmd | 03b499e | Anthony Hung | 2020-01-13 | Add counts of significant DE Genes |
| html | 03b499e | Anthony Hung | 2020-01-13 | Add counts of significant DE Genes |
| html | 5eb29e8 | Anthony Hung | 2020-01-13 | Knit analysis files |
| html | f1425df | Anthony Hung | 2020-01-13 | Correct CPM Cutoff strategy |
| html | bd0e9ca | Anthony Hung | 2020-01-11 | Re-run analyses with real data |
| Rmd | 63f5760 | Anthony Hung | 2020-01-09 | Knit analysis files |
| html | 63f5760 | Anthony Hung | 2020-01-09 | Knit analysis files |
| Rmd | 5c249f2 | Anthony Hung | 2020-01-07 | Add functions to plot Limma results |
| html | 5c249f2 | Anthony Hung | 2020-01-07 | Add functions to plot Limma results |
| Rmd | ea5b72e | Anthony Hung | 2020-01-06 | Revise limma commands |
| html | ea5b72e | Anthony Hung | 2020-01-06 | Revise limma commands |
| Rmd | d80f643 | Anthony Hung | 2020-01-06 | Run Limma: |
| Rmd | b7948f0 | Anthony Hung | 2020-01-06 | DE analysis design matrix |
| Rmd | 9301706 | Anthony Hung | 2019-12-16 | build analysis files |
| Rmd | bb98d14 | Anthony Hung | 2019-12-16 | Add sampleinfo file |
| Rmd | c1f7cf7 | Anthony Hung | 2019-12-16 | Start up additional analysis files |
library("limma")
library("plyr")
library("edgeR")
library("dplyr")
library("tidyr")
library("ashr")
library("ggplot2")
library("cowplot")
library("VennDiagram")
library("RUVSeq")
library("topGO")
#load in filtered count data, RUVs output
filt_counts <- readRDS("data/filtered_counts.rds")
filt_counts <- filt_counts$counts
RUVsOut <- readRDS("data/RUVsOut.rds")
# load gene annotations
gene_anno <- read.delim("data/gene-annotation.txt",
sep = "\t")
# load in reordered sample information
sampleinfo <- readRDS("data/Sample.info.RNAseq.reordered.csv")
# #randomly sample 1 replicate from each set of 3 replicates
# individuals <- c("18855", "18856", "19160")
# treatments <- c("S", "U")
#
# set.seed(1234)
# selected_samples <- c()
# for(i in c("18856", "18855")){
# for(t in treatments){
# sample_num <- base::sample(c(1:3), 1)
# selected_samples <- c(selected_samples, paste0(i, sep = "_", sample_num, sep = "_", t))
# }
# }
#
# sample_num <- base::sample(c(2:3), 1)
# selected_samples <- c(selected_samples, paste0("19160", sep = "_", sample_num, sep = "_", "U"))
# sample_num <- base::sample(c(1:3), 1)
# selected_samples <- c(selected_samples, paste0("19160", sep = "_", sample_num, sep = "_", "S"))
#
# selected_samples
# randomly_sampled_indices <- match(selected_samples, colnames(filt_counts))
#
# single_filt_counts <- filt_counts[,randomly_sampled_indices]
# anno <- pData(RUVsOut)[randomly_sampled_indices,]
single_filt_counts <- filt_counts
anno <- pData(RUVsOut)
x <- paste0(anno$Individual, anno$treatment)
anno$LibraryPrepBatch <- factor(anno$LibraryPrepBatch, levels = c("1", "2"))
anno$Replicate <- factor(anno$Replicate, levels = c("1", "2", "3"))
design <- model.matrix(~treatment + Sex + Individual + W_1 + W_2 + RIN,
data = anno)
colnames(design) <- gsub("treatment", "", colnames(design))
colnames(design)
[1] "(Intercept)" "Unstrain" "SexM"
[4] "IndividualNA18856 " "IndividualNA19160 " "W_1"
[7] "W_2" "RIN"
# Model individual as a random effect (use duplicate correlation function); treatment as a fixed effect, covariates = RUV factors
# Because of issues with duplicateCorrelation with the number of covariates I include in the design matrix, I elect intead to include all samples (all technical replicates) and use duplicateCorrelation for technical replicates and instead include Individual as a fixed effect in the linear model
# Recommended to run both voom and duplicateCorrelation twice.
# https://support.bioconductor.org/p/59700/#67620
#TMM Normalization
y <- DGEList(single_filt_counts)
y <- calcNormFactors(y, method = "TMM")
#Voom for differential expression
v1 <- voom(y, design)
Coefficients not estimable: IndividualNA19160
Warning: Partial NA coefficients for 10517 probe(s)
corfit1 <- duplicateCorrelation(v1, design, block = x)
Coefficients not estimable: IndividualNA19160
Warning in glmgam.fit(dx, dy, coef.start = start, tol = tol, maxit =
maxit, : Too much damping - convergence tolerance not achievable
corfit1$consensus.correlation
[1] 0.3330516
v2 <- voom(y, design, block = x, correlation = corfit1$consensus.correlation)
Coefficients not estimable: IndividualNA19160
Warning: Partial NA coefficients for 10517 probe(s)
corfit2 <- duplicateCorrelation(v2, design, block = x)
Coefficients not estimable: IndividualNA19160
Warning in glmgam.fit(dx, dy, coef.start = start, tol = tol, maxit =
maxit, : Too much damping - convergence tolerance not achievable
Warning in glmgam.fit(dx, dy, coef.start = start, tol = tol, maxit =
maxit, : Too much damping - convergence tolerance not achievable
corfit2$consensus.correlation
[1] 0.3331473
fit <- lmFit(v2, design, block = x,
correlation = corfit2$consensus.correlation)
Coefficients not estimable: IndividualNA19160
Warning: Partial NA coefficients for 10517 probe(s)
fit <- eBayes(fit)
Warning in .ebayes(fit = fit, proportion = proportion, stdev.coef.lim =
stdev.coef.lim, : Estimation of var.prior failed - set to default value
saveRDS(v2, "output/voom_results.rds")
get_results <- function(x, number = nrow(x$coefficients), sort.by = "none",
...) {
# x - object MArrayLM from eBayes output
# ... - additional arguments passed to topTable
stopifnot(class(x) == "MArrayLM")
results <- topTable(x, number = number, sort.by = sort.by, ...)
return(results)
}
top_treatment <- get_results(fit, coef = "Unstrain", sort.by = "B")
head(top_treatment)
logFC AveExpr t P.Value adj.P.Val
ENSG00000102317 0.9987454 6.675008 9.623281 1.524391e-08 0.0001603202
ENSG00000104368 -3.0460584 3.590530 -8.010949 2.308420e-07 0.0012138827
ENSG00000100292 -1.0889277 5.415850 -7.351254 7.722353e-07 0.0027071997
ENSG00000181019 -0.7867957 5.816648 -6.684738 2.776203e-06 0.0049479401
ENSG00000005102 1.1806718 2.864059 6.669238 2.862102e-06 0.0049479401
ENSG00000080824 -0.5223356 8.869466 -6.453824 4.386006e-06 0.0049479401
B
ENSG00000102317 9.815807
ENSG00000104368 6.715829
ENSG00000100292 6.075909
ENSG00000181019 4.870571
ENSG00000005102 4.446004
ENSG00000080824 4.428583
results_treatment <- get_results(fit, coef = "Unstrain",
number = nrow(filt_counts), sort = "none")
ma_treatment <- ggplot(data.frame(Amean = fit$Amean, logFC = fit$coef[, "Unstrain"]),
aes(x = Amean, y = logFC)) +
geom_point() +
labs(x = "Average expression level", y = "Log fold change",
title = "Treatment effect")
ma_treatment

| Version | Author | Date |
|---|---|---|
| f906578 | Anthony Hung | 2020-11-29 |
| 824d084 | Anthony Hung | 2020-11-29 |
| c2ccaf4 | Anthony Hung | 2020-11-29 |
| 66f54cc | Anthony Hung | 2020-06-21 |
| 2cb3b3f | Anthony Hung | 2020-06-01 |
| 797305a | Anthony Hung | 2020-01-18 |
| cb6a0a5 | Anthony Hung | 2020-01-18 |
| 158fdf3 | Anthony Hung | 2020-01-15 |
| 00a66fb | Anthony Hung | 2020-01-13 |
| a04a1ac | Anthony Hung | 2020-01-13 |
| 03b499e | Anthony Hung | 2020-01-13 |
| 5eb29e8 | Anthony Hung | 2020-01-13 |
| f1425df | Anthony Hung | 2020-01-13 |
| bd0e9ca | Anthony Hung | 2020-01-11 |
| ea5b72e | Anthony Hung | 2020-01-06 |
hist_treatment <- ggplot(results_treatment, aes(x = P.Value)) +
geom_histogram(binwidth = 0.01) +
labs(x = "p-value", y = "Number of genes", title = "Treatment effect")
hist_treatment

| Version | Author | Date |
|---|---|---|
| f906578 | Anthony Hung | 2020-11-29 |
| 824d084 | Anthony Hung | 2020-11-29 |
| c2ccaf4 | Anthony Hung | 2020-11-29 |
| 66f54cc | Anthony Hung | 2020-06-21 |
| 2cb3b3f | Anthony Hung | 2020-06-01 |
| 797305a | Anthony Hung | 2020-01-18 |
| cb6a0a5 | Anthony Hung | 2020-01-18 |
| 158fdf3 | Anthony Hung | 2020-01-15 |
| 00a66fb | Anthony Hung | 2020-01-13 |
| a04a1ac | Anthony Hung | 2020-01-13 |
| 03b499e | Anthony Hung | 2020-01-13 |
| 5eb29e8 | Anthony Hung | 2020-01-13 |
| f1425df | Anthony Hung | 2020-01-13 |
| bd0e9ca | Anthony Hung | 2020-01-11 |
| ea5b72e | Anthony Hung | 2020-01-06 |
Boxplot function.
plot_gene <- function(v, g) {
# v - An EList object containing log2 counts per million
# g - character vector of a single gene
stopifnot(class(v) == "EList",
is.character(g), length(g) == 1)
library("tidyr")
single_gene <- v$E[g, ]
single_gene_long <- as.data.frame(single_gene)
colnames(single_gene_long) <- "log2cpm"
single_gene_long$sample <- rownames(single_gene_long)
single_gene_long <- separate(single_gene_long, col = "sample", sep = "_",
into = c("Individual", "Replicate", "Treatment"))
single_gene_long$Treatment <- factor(single_gene_long$Treatment, levels = c("S", "U"))
single_gene_long$Treatment <- revalue(single_gene_long$Treatment, c("S"="Strain", "U"="No Strain"))
ggplot(single_gene_long, aes(x = Treatment, y = log2cpm, fill = Treatment)) +
labs(title = g, x = "Treatment", y = expression("Expression level (" * log[2] * " cpm)")) +
geom_dotplot(binaxis = "y", stackdir = "center") +
stat_summary(fun.data = "mean_sdl", fun.args = list(mult=1))
}
Treatment
#rename top_treatment genes to gene symbols for easier interpretation
top_treatment_top4 <- gene_anno$external_gene_name[match(head(rownames(top_treatment), n=4), gene_anno$ensembl_gene_id)]
top_treatment_top4
[1] RBM3 PLAT HMOX1 NQO1
56858 Levels: A1BG A1BG-AS1 A1CF A2M A2M-AS1 A2ML1 A2ML1-AS1 ... ZZEF1
p_treatment_1 <- plot_gene(v2, rownames(top_treatment)[1])
p_treatment_2 <- plot_gene(v2, rownames(top_treatment)[2])
p_treatment_3 <- plot_gene(v2, rownames(top_treatment)[3])
p_treatment_4 <- plot_gene(v2, rownames(top_treatment)[4])
ggsave("fig-limma-treatment-hit-1.eps", plot = p_treatment_1,
width = 7, height = 7, units = "in")
`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.
plot_grid(p_treatment_1, p_treatment_2, p_treatment_3, p_treatment_4)
`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.
`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.
`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.
`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

| Version | Author | Date |
|---|---|---|
| f906578 | Anthony Hung | 2020-11-29 |
| 824d084 | Anthony Hung | 2020-11-29 |
| c2ccaf4 | Anthony Hung | 2020-11-29 |
| 66f54cc | Anthony Hung | 2020-06-21 |
| 2cb3b3f | Anthony Hung | 2020-06-01 |
| e6d3fad | Anthony Hung | 2020-04-05 |
| 797305a | Anthony Hung | 2020-01-18 |
| cb6a0a5 | Anthony Hung | 2020-01-18 |
| 158fdf3 | Anthony Hung | 2020-01-15 |
| 00a66fb | Anthony Hung | 2020-01-13 |
| a04a1ac | Anthony Hung | 2020-01-13 |
| 03b499e | Anthony Hung | 2020-01-13 |
| 5eb29e8 | Anthony Hung | 2020-01-13 |
| f1425df | Anthony Hung | 2020-01-13 |
| bd0e9ca | Anthony Hung | 2020-01-11 |
| 5c249f2 | Anthony Hung | 2020-01-07 |
Treatment effect.
run_ash <- function(x, coef) {
# Perform multiple testing correction with adaptive shrinkage (ASH)
#
# x - object MArrayLM from eBayes output
# coef - coefficient tested by eBayes
stopifnot(class(x) == "MArrayLM", coef %in% colnames(x$coefficients))
result <- ash(betahat = x$coefficients[, coef],
sebetahat = x$stdev.unscaled[, coef] * sqrt(x$s2.post),
df = x$df.total[1])
return(result)
}
ash_treatment <- run_ash(fit, "Unstrain")
class(ash_treatment)
[1] "ash"
names(ash_treatment)
[1] "fitted_g" "loglik" "logLR" "data" "result" "call"
sum(ash_treatment$result$svalue < .05)
[1] 917
hist(ash_treatment$result$svalue)

| Version | Author | Date |
|---|---|---|
| f906578 | Anthony Hung | 2020-11-29 |
| 824d084 | Anthony Hung | 2020-11-29 |
| c2ccaf4 | Anthony Hung | 2020-11-29 |
| 66f54cc | Anthony Hung | 2020-06-21 |
| 2cb3b3f | Anthony Hung | 2020-06-01 |
| 797305a | Anthony Hung | 2020-01-18 |
| cb6a0a5 | Anthony Hung | 2020-01-18 |
| 158fdf3 | Anthony Hung | 2020-01-15 |
| 00a66fb | Anthony Hung | 2020-01-13 |
| a04a1ac | Anthony Hung | 2020-01-13 |
| 03b499e | Anthony Hung | 2020-01-13 |
| 5eb29e8 | Anthony Hung | 2020-01-13 |
| f1425df | Anthony Hung | 2020-01-13 |
| bd0e9ca | Anthony Hung | 2020-01-11 |
| 5c249f2 | Anthony Hung | 2020-01-07 |
plot_ma <- function(x, qval) {
# Create MA plot.
#
# x - data frame with topTable and ASH output
# (columns logFC, AveExpr, and qvalue)
# qval - qvalue cutoff for calling a gene DE
#
stopifnot(is.data.frame(x), c("logFC", "AveExpr", "qvalue") %in% colnames(x),
is.numeric(qval), qval <= 1, qval >= 0)
x$highlight <- ifelse(x$qvalue < qval, "darkred", "gray75")
x$highlight <- factor(x$highlight, levels = c("darkred", "gray75"))
ggplot(x, aes(x = AveExpr, y = logFC, color = highlight, shape = highlight)) +
geom_point() +
labs(x = "Average expression level", y = "Log fold change") +
scale_color_identity(drop = FALSE) +
scale_shape_manual(values = c(16, 1), drop = FALSE) +
theme(legend.position = "none")
# scale_color_gradient(low = "red", high = "white", limits = c(0, 0.25))
}
plot_volcano <- function(x, qval) {
# Create volcano plot.
#
# x - data frame with topTable and ASH output
# (columns logFC, P.Value, and qvalue)
# qval - qvalue cutoff for calling a gene DE
#
stopifnot(is.data.frame(x), c("logFC", "P.Value", "qvalue") %in% colnames(x),
is.numeric(qval), qval <= 1, qval >= 0)
x$highlight <- ifelse(x$qvalue < qval, "darkred", "gray75")
x$highlight <- factor(x$highlight, levels = c("darkred", "gray75"))
ggplot(x, aes(x = logFC, y = -log10(P.Value), color = highlight)) +
geom_point(shape = 1) +
labs(x = "Log fold change",
y = expression(-log[10] * " p-value")) +
scale_color_identity(drop = FALSE) +
theme(legend.position = "none")
}
plot_pval_hist <- function(x, qval) {
# Create histogram of p-values.
#
# x - data frame with topTable and ash output (columns P.Value and qvalue)
# qval - qvalue cutoff for calling a gene DE
#
stopifnot(is.data.frame(x), c("P.Value", "qvalue") %in% colnames(x))
x$highlight <- ifelse(x$qvalue < qval, "darkred", "gray75")
x$highlight <- factor(x$highlight, levels = c("darkred", "gray75"))
ggplot(x, aes(x = P.Value, fill = highlight)) +
geom_histogram(position = "stack", binwidth = 0.01) +
scale_fill_identity(drop = FALSE) +
labs(x = "p-value", y = "Number of genes")
}
tests <- colnames(fit$coefficients)
results <- vector(length = length(tests), mode = "list")
names(results) <- tests
for (test in tests[c(1:4, 6:8)]) {
# Extract limma results
print(test)
results[[test]] <- get_results(fit, coef = test)
# Add mutliple testing correction with ASH
output_ash <- run_ash(fit, coef = test)$result
results[[test]] <- cbind(results[[test]], lfsr = output_ash$lfsr,
lfdr = output_ash$lfdr, qvalue = output_ash$qvalue,
svalue = output_ash$svalue)
}
[1] "(Intercept)"
[1] "Unstrain"
[1] "SexM"
[1] "IndividualNA18856 "
[1] "W_1"
[1] "W_2"
[1] "RIN"
#FDR 0.1
plot_ma(results[["Unstrain"]], 0.1)

| Version | Author | Date |
|---|---|---|
| f906578 | Anthony Hung | 2020-11-29 |
| 824d084 | Anthony Hung | 2020-11-29 |
| c2ccaf4 | Anthony Hung | 2020-11-29 |
| 66f54cc | Anthony Hung | 2020-06-21 |
| 2cb3b3f | Anthony Hung | 2020-06-01 |
| 797305a | Anthony Hung | 2020-01-18 |
| cb6a0a5 | Anthony Hung | 2020-01-18 |
| 158fdf3 | Anthony Hung | 2020-01-15 |
| 00a66fb | Anthony Hung | 2020-01-13 |
| a04a1ac | Anthony Hung | 2020-01-13 |
| 03b499e | Anthony Hung | 2020-01-13 |
| 5eb29e8 | Anthony Hung | 2020-01-13 |
| f1425df | Anthony Hung | 2020-01-13 |
| bd0e9ca | Anthony Hung | 2020-01-11 |
| 5c249f2 | Anthony Hung | 2020-01-07 |
plot_volcano(results[["Unstrain"]], 0.1)

| Version | Author | Date |
|---|---|---|
| f906578 | Anthony Hung | 2020-11-29 |
| 824d084 | Anthony Hung | 2020-11-29 |
| c2ccaf4 | Anthony Hung | 2020-11-29 |
| 66f54cc | Anthony Hung | 2020-06-21 |
| 2cb3b3f | Anthony Hung | 2020-06-01 |
| 797305a | Anthony Hung | 2020-01-18 |
| cb6a0a5 | Anthony Hung | 2020-01-18 |
| 158fdf3 | Anthony Hung | 2020-01-15 |
| 00a66fb | Anthony Hung | 2020-01-13 |
| a04a1ac | Anthony Hung | 2020-01-13 |
| 03b499e | Anthony Hung | 2020-01-13 |
| 5eb29e8 | Anthony Hung | 2020-01-13 |
| f1425df | Anthony Hung | 2020-01-13 |
| bd0e9ca | Anthony Hung | 2020-01-11 |
| 5c249f2 | Anthony Hung | 2020-01-07 |
plot_pval_hist(results[["Unstrain"]], 0.1)

| Version | Author | Date |
|---|---|---|
| f906578 | Anthony Hung | 2020-11-29 |
| 824d084 | Anthony Hung | 2020-11-29 |
| c2ccaf4 | Anthony Hung | 2020-11-29 |
| 66f54cc | Anthony Hung | 2020-06-21 |
| 2cb3b3f | Anthony Hung | 2020-06-01 |
| 797305a | Anthony Hung | 2020-01-18 |
| cb6a0a5 | Anthony Hung | 2020-01-18 |
| 158fdf3 | Anthony Hung | 2020-01-15 |
| 00a66fb | Anthony Hung | 2020-01-13 |
| a04a1ac | Anthony Hung | 2020-01-13 |
| 03b499e | Anthony Hung | 2020-01-13 |
| 5eb29e8 | Anthony Hung | 2020-01-13 |
| f1425df | Anthony Hung | 2020-01-13 |
| bd0e9ca | Anthony Hung | 2020-01-11 |
| 5c249f2 | Anthony Hung | 2020-01-07 |
table(results[["Unstrain"]]$qvalue < 0.1)
FALSE TRUE
8000 2517
significant_genes_10 <- row.names(results[["Unstrain"]])[results[["Unstrain"]]$qvalue < 0.1]
significant_symbols_10 <- gene_anno$external_gene_name[match(significant_genes_10, gene_anno$ensembl_gene_id)]
head(significant_symbols_10)
[1] LINC01128 AGRN DVL1 MXRA8 AURKAIP1 AL391244.1
56858 Levels: A1BG A1BG-AS1 A1CF A2M A2M-AS1 A2ML1 A2ML1-AS1 ... ZZEF1
significant_anno_10 <- gene_anno[match(significant_genes_10, gene_anno$ensembl_gene_id),]
#FDR 0.05
plot_ma(results[["Unstrain"]], 0.05)

| Version | Author | Date |
|---|---|---|
| f906578 | Anthony Hung | 2020-11-29 |
| 824d084 | Anthony Hung | 2020-11-29 |
| c2ccaf4 | Anthony Hung | 2020-11-29 |
| 66f54cc | Anthony Hung | 2020-06-21 |
| 2cb3b3f | Anthony Hung | 2020-06-01 |
| 797305a | Anthony Hung | 2020-01-18 |
| cb6a0a5 | Anthony Hung | 2020-01-18 |
| 158fdf3 | Anthony Hung | 2020-01-15 |
| 00a66fb | Anthony Hung | 2020-01-13 |
| a04a1ac | Anthony Hung | 2020-01-13 |
| 03b499e | Anthony Hung | 2020-01-13 |
| 5eb29e8 | Anthony Hung | 2020-01-13 |
| f1425df | Anthony Hung | 2020-01-13 |
| bd0e9ca | Anthony Hung | 2020-01-11 |
| 63f5760 | Anthony Hung | 2020-01-09 |
plot_volcano(results[["Unstrain"]], 0.05)

| Version | Author | Date |
|---|---|---|
| f906578 | Anthony Hung | 2020-11-29 |
| 824d084 | Anthony Hung | 2020-11-29 |
| c2ccaf4 | Anthony Hung | 2020-11-29 |
| 66f54cc | Anthony Hung | 2020-06-21 |
| 2cb3b3f | Anthony Hung | 2020-06-01 |
| 797305a | Anthony Hung | 2020-01-18 |
| cb6a0a5 | Anthony Hung | 2020-01-18 |
| 158fdf3 | Anthony Hung | 2020-01-15 |
| 00a66fb | Anthony Hung | 2020-01-13 |
| a04a1ac | Anthony Hung | 2020-01-13 |
| 03b499e | Anthony Hung | 2020-01-13 |
| 5eb29e8 | Anthony Hung | 2020-01-13 |
| f1425df | Anthony Hung | 2020-01-13 |
| bd0e9ca | Anthony Hung | 2020-01-11 |
| 63f5760 | Anthony Hung | 2020-01-09 |
plot_pval_hist(results[["Unstrain"]], 0.05)

| Version | Author | Date |
|---|---|---|
| f906578 | Anthony Hung | 2020-11-29 |
| 824d084 | Anthony Hung | 2020-11-29 |
| c2ccaf4 | Anthony Hung | 2020-11-29 |
| 66f54cc | Anthony Hung | 2020-06-21 |
| 2cb3b3f | Anthony Hung | 2020-06-01 |
| 797305a | Anthony Hung | 2020-01-18 |
| cb6a0a5 | Anthony Hung | 2020-01-18 |
| 158fdf3 | Anthony Hung | 2020-01-15 |
| 00a66fb | Anthony Hung | 2020-01-13 |
| a04a1ac | Anthony Hung | 2020-01-13 |
| 03b499e | Anthony Hung | 2020-01-13 |
| 5eb29e8 | Anthony Hung | 2020-01-13 |
| f1425df | Anthony Hung | 2020-01-13 |
| bd0e9ca | Anthony Hung | 2020-01-11 |
| 63f5760 | Anthony Hung | 2020-01-09 |
table(results[["Unstrain"]]$qvalue < 0.05)
FALSE TRUE
9363 1154
significant_genes_05 <- row.names(results[["Unstrain"]])[results[["Unstrain"]]$qvalue < 0.05]
significant_symbols_05 <- gene_anno$external_gene_name[match(significant_genes_05, gene_anno$ensembl_gene_id)]
head(significant_symbols_05)
[1] DVL1 MRPL20 ATAD3A MMP23B PEX10 CEP104
56858 Levels: A1BG A1BG-AS1 A1CF A2M A2M-AS1 A2ML1 A2ML1-AS1 ... ZZEF1
significant_anno_05 <- gene_anno[match(significant_genes_05, gene_anno$ensembl_gene_id),]
save(significant_genes_05, file="data/DEgenes_FDR05.rda")
#FDR 0.01
plot_ma(results[["Unstrain"]], 0.01)

| Version | Author | Date |
|---|---|---|
| f906578 | Anthony Hung | 2020-11-29 |
| 824d084 | Anthony Hung | 2020-11-29 |
| c2ccaf4 | Anthony Hung | 2020-11-29 |
| 66f54cc | Anthony Hung | 2020-06-21 |
| 2cb3b3f | Anthony Hung | 2020-06-01 |
| 797305a | Anthony Hung | 2020-01-18 |
| cb6a0a5 | Anthony Hung | 2020-01-18 |
| 158fdf3 | Anthony Hung | 2020-01-15 |
| 00a66fb | Anthony Hung | 2020-01-13 |
| a04a1ac | Anthony Hung | 2020-01-13 |
| 03b499e | Anthony Hung | 2020-01-13 |
| 5eb29e8 | Anthony Hung | 2020-01-13 |
| f1425df | Anthony Hung | 2020-01-13 |
| bd0e9ca | Anthony Hung | 2020-01-11 |
| 63f5760 | Anthony Hung | 2020-01-09 |
plot_volcano(results[["Unstrain"]], 0.01)

| Version | Author | Date |
|---|---|---|
| f906578 | Anthony Hung | 2020-11-29 |
| 824d084 | Anthony Hung | 2020-11-29 |
| c2ccaf4 | Anthony Hung | 2020-11-29 |
| 66f54cc | Anthony Hung | 2020-06-21 |
| 2cb3b3f | Anthony Hung | 2020-06-01 |
| 797305a | Anthony Hung | 2020-01-18 |
| cb6a0a5 | Anthony Hung | 2020-01-18 |
| 158fdf3 | Anthony Hung | 2020-01-15 |
| 00a66fb | Anthony Hung | 2020-01-13 |
| a04a1ac | Anthony Hung | 2020-01-13 |
| 03b499e | Anthony Hung | 2020-01-13 |
| 5eb29e8 | Anthony Hung | 2020-01-13 |
| f1425df | Anthony Hung | 2020-01-13 |
| bd0e9ca | Anthony Hung | 2020-01-11 |
| 63f5760 | Anthony Hung | 2020-01-09 |
plot_pval_hist(results[["Unstrain"]], 0.01)

| Version | Author | Date |
|---|---|---|
| f906578 | Anthony Hung | 2020-11-29 |
| 824d084 | Anthony Hung | 2020-11-29 |
| c2ccaf4 | Anthony Hung | 2020-11-29 |
| 66f54cc | Anthony Hung | 2020-06-21 |
| 2cb3b3f | Anthony Hung | 2020-06-01 |
| 797305a | Anthony Hung | 2020-01-18 |
| cb6a0a5 | Anthony Hung | 2020-01-18 |
| 158fdf3 | Anthony Hung | 2020-01-15 |
| 00a66fb | Anthony Hung | 2020-01-13 |
| a04a1ac | Anthony Hung | 2020-01-13 |
| 03b499e | Anthony Hung | 2020-01-13 |
| 5eb29e8 | Anthony Hung | 2020-01-13 |
| f1425df | Anthony Hung | 2020-01-13 |
| bd0e9ca | Anthony Hung | 2020-01-11 |
| 63f5760 | Anthony Hung | 2020-01-09 |
table(results[["Unstrain"]]$qvalue < 0.01)
FALSE TRUE
10347 170
significant_genes_01 <- row.names(results[["Unstrain"]])[results[["Unstrain"]]$qvalue < 0.01]
significant_symbols_01 <- gene_anno$external_gene_name[match(significant_genes_01, gene_anno$ensembl_gene_id)]
head(significant_symbols_01)
[1] TENT5B EYA3 RCC1 SLC6A9 PLK3 PRDX1
56858 Levels: A1BG A1BG-AS1 A1CF A2M A2M-AS1 A2ML1 A2ML1-AS1 ... ZZEF1
significant_anno_01 <- gene_anno[match(significant_genes_01, gene_anno$ensembl_gene_id),]
#abs(logFC) > 1
significant_genes_05_FC1 <- row.names(results[["Unstrain"]])[results[["Unstrain"]]$qvalue < 0.05 & abs(results[["Unstrain"]]$logFC) > 1]
significant_symbols_05_FC1 <- gene_anno$external_gene_name[match(significant_genes_05_FC1, gene_anno$ensembl_gene_id)]
significant_anno_05_FC1 <- gene_anno[match(significant_genes_05_FC1, gene_anno$ensembl_gene_id),]
significant_anno_05_FC1
ensembl_gene_id external_gene_name chromosome_name
31021 ENSG00000232679 LINC01705 1
4381 ENSG00000115221 ITGB6 2
7662 ENSG00000138411 HECW2 2
28538 ENSG00000229056 AC020571.1 2
8570 ENSG00000144485 HES6 2
5175 ENSG00000121577 POPDC2 3
934 ENSG00000065534 MYLK 3
13727 ENSG00000174640 SLCO2A1 3
15279 ENSG00000182585 EPGN 4
4845 ENSG00000118785 SPP1 4
14924 ENSG00000180801 ARSJ 4
13297 ENSG00000172403 SYNPO2 4
9313 ENSG00000150630 VEGFC 4
11274 ENSG00000164171 ITGA2 5
13332 ENSG00000172548 NIPAL4 5
7450 ENSG00000137309 HMGA1 6
28168 ENSG00000228495 LINC01013 6
3341 ENSG00000106631 MYL7 7
10067 ENSG00000157168 NRG1 8
2910 ENSG00000104368 PLAT 8
457 ENSG00000026508 CD44 11
46915 ENSG00000261578 AP003119.3 11
3449 ENSG00000107796 ACTA2 10
7759 ENSG00000139044 B4GALNT3 12
3928 ENSG00000111674 ENO2 12
11706 ENSG00000165891 E2F7 12
10325 ENSG00000159251 ACTC1 15
81 ENSG00000005102 MEOX1 17
5642 ENSG00000125398 SOX9 17
14020 ENSG00000176170 SPHK1 17
2562 ENSG00000101670 LIPG 18
2157 ENSG00000100003 SEC14L2 22
2248 ENSG00000100292 HMOX1 22
description
31021 long intergenic non-protein coding RNA 1705 [Source:HGNC Symbol;Acc:HGNC:52493]
4381 integrin subunit beta 6 [Source:HGNC Symbol;Acc:HGNC:6161]
7662 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:29853]
28538
8570 hes family bHLH transcription factor 6 [Source:HGNC Symbol;Acc:HGNC:18254]
5175 popeye domain containing 2 [Source:HGNC Symbol;Acc:HGNC:17648]
934 myosin light chain kinase [Source:HGNC Symbol;Acc:HGNC:7590]
13727 solute carrier organic anion transporter family member 2A1 [Source:HGNC Symbol;Acc:HGNC:10955]
15279 epithelial mitogen [Source:HGNC Symbol;Acc:HGNC:17470]
4845 secreted phosphoprotein 1 [Source:HGNC Symbol;Acc:HGNC:11255]
14924 arylsulfatase family member J [Source:HGNC Symbol;Acc:HGNC:26286]
13297 synaptopodin 2 [Source:HGNC Symbol;Acc:HGNC:17732]
9313 vascular endothelial growth factor C [Source:HGNC Symbol;Acc:HGNC:12682]
11274 integrin subunit alpha 2 [Source:HGNC Symbol;Acc:HGNC:6137]
13332 NIPA like domain containing 4 [Source:HGNC Symbol;Acc:HGNC:28018]
7450 high mobility group AT-hook 1 [Source:HGNC Symbol;Acc:HGNC:5010]
28168 long intergenic non-protein coding RNA 1013 [Source:HGNC Symbol;Acc:HGNC:48987]
3341 myosin light chain 7 [Source:HGNC Symbol;Acc:HGNC:21719]
10067 neuregulin 1 [Source:HGNC Symbol;Acc:HGNC:7997]
2910 plasminogen activator, tissue type [Source:HGNC Symbol;Acc:HGNC:9051]
457 CD44 molecule (Indian blood group) [Source:HGNC Symbol;Acc:HGNC:1681]
46915
3449 actin, alpha 2, smooth muscle, aorta [Source:HGNC Symbol;Acc:HGNC:130]
7759 beta-1,4-N-acetyl-galactosaminyltransferase 3 [Source:HGNC Symbol;Acc:HGNC:24137]
3928 enolase 2 [Source:HGNC Symbol;Acc:HGNC:3353]
11706 E2F transcription factor 7 [Source:HGNC Symbol;Acc:HGNC:23820]
10325 actin, alpha, cardiac muscle 1 [Source:HGNC Symbol;Acc:HGNC:143]
81 mesenchyme homeobox 1 [Source:HGNC Symbol;Acc:HGNC:7013]
5642 SRY-box 9 [Source:HGNC Symbol;Acc:HGNC:11204]
14020 sphingosine kinase 1 [Source:HGNC Symbol;Acc:HGNC:11240]
2562 lipase G, endothelial type [Source:HGNC Symbol;Acc:HGNC:6623]
2157 SEC14 like lipid binding 2 [Source:HGNC Symbol;Acc:HGNC:10699]
2248 heme oxygenase 1 [Source:HGNC Symbol;Acc:HGNC:5013]
start_position end_position
31021 222041705 222064763
4381 160099666 160200313
7662 196189099 196593692
28538 196260024 196264204
8570 238238267 238240662
5175 119636457 119665324
934 123610049 123884331
13727 133932696 134052184
15279 74308473 74315307
4845 87975650 87983426
14924 113900284 113979727
13297 118850688 119061247
9313 176683538 176792727
11274 52989326 53094779
13332 157460019 157474717
7450 34236873 34246231
28168 132131915 132169374
3341 44138864 44141332
10067 31639386 32767959
2910 42175233 42207724
457 35138870 35232402
46915 76800364 76804555
3449 88935074 88991339
7759 460364 563509
3928 6913745 6923698
11706 77021247 77065580
10325 34788096 34796139
81 43640388 43661954
5642 72121020 72126420
14020 76376584 76387860
2562 49560699 49599182
2157 30396857 30425317
2248 35380361 35394214
phenotype
31021
4381 Alopecia-intellectual disability syndrome;Hypocalcified amelogenesis imperfecta;Hypoplastic amelogenesis imperfecta;AMELOGENESIS IMPERFECTA TYPE IH
7662 HECW2;NEURODEVELOPMENTAL DISORDER WITH HYPOTONIA SEIZURES AND ABSENT LANGUAGE
28538
8570
5175
934 Megacystis-microcolon-intestinal hypoperistalsis syndrome;Familial thoracic aortic aneurysm and aortic dissection;AORTIC ANEURYSM FAMILIAL THORACIC 7
13727 Chronic enteropathy associated with SLCO2A1 gene;Pachydermoperiostosis;HYPERTROPHIC OSTEOARTHROPATHY PRIMARY AUTOSOMAL RECESSIVE 2
15279
4845 Pediatric systemic lupus erythematosus
14924
13297
9313 Milroy Disease;Lymphedema hereditary id
11274 Fetal and neonatal alloimmune thrombocytopenia;BLEEDING DISORDER PLATELET-TYPE 9
13332 Congenital nonbullous ichthyosiform erythroderma;Lamellar ichthyosis;ICHTHYOSIS CONGENITAL AUTOSOMAL RECESSIVE 6
7450 DIABETES MELLITUS NONINSULIN-DEPENDENT
28168
3341
10067 SCHIZOPHRENIA 6
2910 Lethal hydranencephaly-diaphragmatic hernia syndrome;THROMBOPHILIA FAMILIAL DUE TO DECREASED RELEASE OF TISSUE PLASMINOGENACTIVATOR
457 BLOOD GROUP INDIAN SYSTEM
46915
3449 AORTIC ANEURYSM FAMILIAL THORACIC 6;Moyamoya disease 5;Familial thoracic aortic aneurysm and aortic dissection;Moyamoya disease;MULTISYSTEMIC SMOOTH MUSCLE DYSFUNCTION SYNDROME
7759
3928
11706
10325 Atrial septal defect ostium secundum type;Familial isolated dilated cardiomyopathy;Left ventricular noncompaction;ATRIAL SEPTAL DEFECT 5;Cardiomyopathy dilated 1R;CARDIOMYOPATHY FAMILIAL HYPERTROPHIC 11
81 Klippel-Feil Anomaly;Isolated Klippel-Feil syndrome;KLIPPEL-FEIL SYNDROME 2 AUTOSOMAL RECESSIVE
5642 CAMPOMELIC DYSPLASIA;PIERRE ROBIN SEQUENCE;46XX ovotesticular disorder of sex development;46XX testicular disorder of sex development;46XY complete gonadal dysgenesis;46XY partial gonadal dysgenesis;Isolated Pierre Robin syndrome;46XY SEX REVERSAL 10
14020
2562
2157
2248 HEME OXYGENASE 1 DEFICIENCY;PULMONARY DISEASE CHRONIC OBSTRUCTIVE
go_id
31021
4381 GO:0016020;GO:0016021;GO:0005813;GO:0005654;GO:0007155;GO:0030054;GO:0005515;GO:0007229;GO:0007160;GO:0008305;GO:0046718;GO:0005925;GO:0005886;GO:0038023;GO:0005178;GO:0016032;GO:0030198;GO:0001618;GO:0043235;GO:0033627;GO:0006954;GO:0038044;GO:0009897;GO:0034685;
7662 ;GO:0005737;GO:0016740;GO:0016567;GO:0004842;GO:0005856;GO:0005819;GO:0061630;GO:0043161;GO:0005515;GO:0072686;GO:0030071
28538
8570 GO:0005634;GO:0046983;GO:0006355;GO:0006351;GO:0003677;GO:0007275;GO:0030154;GO:0000981;GO:0008134;GO:0003700;GO:0003712;GO:0005667;GO:0000977;GO:0001227;GO:0000122;GO:0006357;GO:0007399;
5175 ;GO:0016020;GO:0016021;GO:0005886;GO:0003674;GO:0008150;GO:0042383;GO:0002027
934 GO:0060414;GO:0005737;GO:0046872;GO:0000166;GO:0004672;GO:0004674;GO:0005524;GO:0006468;GO:0016740;GO:0016301;GO:0016310;GO:0003779;GO:0005886;GO:0015629;GO:0005856;GO:0042995;GO:0005515;GO:0005516;GO:0005829;GO:0030027;GO:0001725;GO:0032154;GO:0030335;GO:0051928;GO:0006936;GO:0006939;GO:0090303;GO:0032060;GO:0004687;GO:0014820;GO:0071476;
13727 GO:0016020;GO:0016021;GO:0005215;GO:0055085;GO:0005886;GO:0015347;GO:0043252;GO:0005887;GO:0005319;GO:0006869;GO:0015132;GO:0015732;GO:0006811;
15279 GO:0005622;GO:0016020;GO:0016021;GO:0008083;GO:0005576;GO:0005615;GO:0008284;GO:0005887;GO:0005154;GO:0001525;GO:0043406;GO:0045741;GO:0045840;GO:0000187;GO:0050679;GO:0000165;
4845 ;GO:0005794;GO:0007155;GO:0001503;GO:0005615;GO:0007566;GO:0010469;GO:0005515;GO:0005576;GO:0005125;GO:0070062;GO:0031214;GO:0044267;GO:0043687;GO:0005788;GO:0030198;GO:0045893;GO:0033280;GO:0046697;GO:0050840;GO:0001649;GO:0006710;GO:0045780;GO:0071394;GO:2000866;GO:0006954;GO:0010033;GO:0030154;GO:0048545;GO:0048685;GO:0031982;GO:0042995;GO:0048471
14924 GO:0046872;GO:0016787;GO:0003824;GO:0008152;GO:0005576;GO:0008484;GO:0005788;GO:0004065;GO:0016020;GO:0016021;
13297 GO:0005634;GO:0005737;GO:0043231;GO:0005829;GO:0003779;GO:0015629;GO:0005856;GO:0005515;GO:0030054;GO:0005925;GO:0001725;GO:0030018;GO:0071889;GO:0031005;GO:0032233;GO:0051393;GO:0051371;GO:0000045;GO:0099023;
9313 GO:0016020;GO:0008083;GO:0010469;GO:0005515;GO:0005576;GO:0007275;GO:0030154;GO:0005615;GO:0050918;GO:0001525;GO:0051781;GO:0007165;GO:0002576;GO:0031093;GO:0048010;GO:0042056;GO:0043185;GO:0001666;GO:0001938;GO:0008284;GO:0045766;GO:0050930;GO:0060754;GO:0001954;GO:0002052;GO:0006929;GO:0008285;GO:0009887;GO:0016331;GO:0030947;GO:0031954;GO:0042493;GO:0043536;GO:0045776;GO:0050679;GO:0050714;GO:0050731;GO:1901492;GO:1990830;
11274 GO:0016020;GO:0016021;GO:0046872;GO:0007155;GO:0005515;GO:0009986;GO:0007229;GO:0008305;GO:0046718;GO:0005925;GO:0005886;GO:0016032;GO:0007596;GO:0030198;GO:0044877;GO:0009887;GO:0007160;GO:0001618;GO:0005518;GO:0031589;GO:0001540;GO:0098639;GO:0033627;GO:0048333;GO:0034666;GO:0005178;GO:0038064;GO:0043236;GO:0043395;GO:0046982;GO:0001666;GO:0002687;GO:0006929;GO:0006971;GO:0007565;GO:0008283;GO:0010634;GO:0010694;GO:0014070;GO:0014075;GO:0014850;GO:0014911;GO:0030879;GO:0031346;GO:0032967;GO:0033343;GO:0033591;GO:0038065;GO:0042060;GO:0042493;GO:0043388;GO:0043589;GO:0045184;GO:0045727;GO:0045785;GO:0045987;GO:0048041;GO:0048661;GO:0050729;GO:0050927;GO:0050966;GO:0051971;GO:0060100;GO:0070365;GO:0071107;GO:0071260;GO:0071392;GO:0071407;GO:0005634;GO:0009897;GO:0030424;GO:0042995;GO:0043679;GO:0045178;GO:0048471;
13332 GO:0016020;GO:0016021;GO:0006811;GO:0015095;GO:0015693;GO:1903830;
7450 GO:0045944;GO:0005634;GO:0006355;GO:0006366;GO:0006351;GO:0003677;GO:0005694;GO:0000785;GO:0005515;GO:0030374;GO:0045893;GO:0005925;GO:0003700;GO:0000981;GO:0005654;GO:0005829;GO:0008285;GO:0042974;GO:0009615;GO:0006284;GO:0019899;GO:0008134;GO:0045892;GO:0003682;GO:0090575;GO:0065003;GO:0005667;GO:0051169;GO:0075713;GO:0001205;GO:0006337;GO:0046965;GO:0001158;GO:0003680;GO:0003906;GO:0042975;GO:0006268;GO:0031936;GO:0035986;GO:0090402;GO:2000774;GO:0035985;
28168
3341 GO:0005509;GO:0046872;GO:0016459;GO:0005515;GO:0005829;GO:0006936;GO:0031672;GO:0043197;
10067 GO:0016020;GO:0016021;GO:0005654;GO:0005102;GO:0009790;GO:0005634;GO:0018108;GO:0008083;GO:0010469;GO:0005886;GO:0005576;GO:0005615;GO:0046854;GO:0007171;GO:0008284;GO:0005178;GO:0046934;GO:0051897;GO:0005088;GO:0000165;GO:0030971;GO:0005125;GO:0004713;GO:0038128;GO:2000145;GO:0032148;GO:0055007;GO:0000187;GO:0014032;GO:0045892;GO:0007399;GO:0030297;GO:0051048;GO:0042060;GO:0030879;GO:0003222;GO:0043125;GO:0038127;GO:0060956;GO:0003712;GO:0007154;GO:0038129;GO:0043496;GO:0043497;GO:0051155;GO:0060379;;GO:0005176;GO:0045499;GO:0000902;GO:0001964;GO:0003161;GO:0007416;GO:0007420;GO:0007422;GO:0007507;GO:0007517;GO:0007626;GO:0008366;GO:0010001;GO:0010628;GO:0014068;GO:0016477;GO:0021781;GO:0021842;GO:0022008;GO:0031643;GO:0042177;GO:0045213;GO:0045595;GO:0045773;GO:0045860;GO:0045944;GO:0046579;GO:0048663;GO:0048709;GO:0048738;GO:0050731;GO:0060999;GO:0070886;GO:2000010;GO:2000727;GO:2001223;GO:0005737;GO:0005887;GO:0009897;GO:0030424;GO:0030425;GO:0030673;GO:0031594;GO:0044297;GO:0045202
2910 GO:0005737;GO:0016787;GO:0005576;GO:0008233;GO:0006508;GO:0004252;GO:0005615;GO:0008236;GO:0005515;GO:0070062;GO:0005102;GO:0031639;GO:0009986;GO:0007596;GO:0006464;GO:0045861;GO:0042730;GO:0051219;GO:0001666;GO:0014909;GO:0048008;GO:0030141;GO:0045177;
457 GO:0070374;GO:0016020;GO:0016021;GO:0005794;GO:0005829;GO:0007155;GO:0005886;GO:0042995;GO:0005515;GO:0005540;GO:0051216;GO:0009986;GO:0005925;GO:0050900;GO:0005887;GO:0070062;GO:0005902;GO:0043312;GO:0016324;GO:0033138;GO:0043066;GO:0050731;GO:0030667;GO:0030198;GO:0043154;GO:0060333;GO:0044319;GO:0022617;GO:0007160;GO:0044344;GO:0098609;GO:0005518;GO:0043518;GO:1902166;GO:0030214;GO:0034116;GO:0070487;GO:0004896;GO:0035692;GO:0031258;GO:2000392;GO:1900625;;GO:0033674
46915
3449 GO:0005737;GO:0000166;GO:0005524;GO:0005856;GO:0005615;GO:0005829;GO:0070062;GO:0009615;GO:0032991;GO:0010628;GO:0019901;GO:0008217;GO:0014829;GO:0090131;GO:0015629;GO:0030027;GO:0030175;GO:0030485;GO:0044297;GO:0006936;GO:0072144;
7759 GO:0016020;GO:0016021;GO:0016740;GO:0005794;GO:0008376;GO:0032580;GO:0033842;
3928 GO:0000287;GO:0006096;GO:0004634;GO:0000015;GO:0005737;GO:0016020;GO:0046872;GO:0005829;GO:0016829;GO:0005886;GO:0005515;GO:0005615;GO:0070062;GO:0006094;GO:0061621;;GO:0001917;GO:0005622;GO:0043025;GO:0043204;GO:0043209
11706 GO:0005634;GO:0016607;GO:0006355;GO:0006351;GO:0003677;GO:0003700;GO:0005515;GO:0006974;GO:0007049;GO:0005667;GO:0042802;GO:0000122;GO:0000981;GO:0000987;GO:0003714;GO:0001890;GO:0002040;GO:0006977;GO:0032466;GO:0032877;GO:0045944;GO:0060707;GO:0060718;GO:0070365;GO:0005654;GO:0008285;GO:0030330;GO:2000134;GO:0000977;GO:0000978;GO:0001227;GO:0071930;
10325 GO:0005737;GO:0016020;GO:0000166;GO:0005524;GO:0005856;GO:0005615;GO:0005925;GO:0005829;GO:0070062;GO:0016887;GO:0072562;GO:0031674;GO:0010628;GO:0090131;GO:0030027;GO:0030175;GO:0044297;GO:0005884;GO:0017022;GO:0030049;GO:0030017;GO:0030048;GO:0060047;GO:0055003;GO:0055008;GO:0031032;GO:0030240;GO:0033275;GO:0042493;GO:0043066;GO:0045471;GO:0060048;GO:0070252;GO:0042643;
81 GO:0005634;GO:0005737;GO:0006355;GO:0006366;GO:0006351;GO:0003677;GO:0003700;GO:0043565;GO:0005515;GO:0007275;GO:0000981;GO:0045944;GO:0003674;GO:0008150;GO:0001077;GO:0061053;GO:0000978;GO:0003682;GO:0071837;GO:0001757;GO:0060218;GO:0061056
5642 GO:0045944;GO:0005634;GO:0005654;GO:0006355;GO:0006351;GO:0003677;GO:0003700;GO:0006334;GO:0000981;GO:0005515;GO:0007165;GO:0051216;GO:0045893;GO:0007283;GO:0001934;GO:0001501;GO:0090090;GO:0003682;GO:0008584;GO:0032991;GO:0071347;GO:0071260;GO:0008284;GO:0042127;GO:0042981;GO:0043066;GO:0070371;GO:0001502;GO:0032332;GO:0045892;GO:0006367;GO:0004672;GO:0019933;GO:0071560;GO:0007173;GO:0045662;GO:0060041;GO:0000978;GO:0002062;GO:1902894;GO:0071300;GO:0065003;GO:0050680;GO:0006338;GO:0001158;GO:0001077;GO:0030850;GO:0010634;GO:0050679;GO:0014068;GO:0034236;GO:0001708;GO:0002053;GO:0090103;GO:0060008;GO:0001894;GO:0071364;GO:0030279;GO:0070168;GO:0071504;GO:0003705;GO:0001837;GO:0090190;GO:0001942;GO:0003203;GO:0090184;GO:2000020;GO:0030916;GO:0061036;GO:2000741;GO:0032331;GO:0035019;GO:0000976;GO:0001228;GO:0008013;GO:0035326;GO:0043425;GO:0043565;GO:0044212;GO:0046982;GO:0097157;GO:0000122;GO:0001503;GO:0001658;GO:0002063;GO:0002683;GO:0003170;GO:0003179;GO:0003188;GO:0003413;GO:0003415;GO:0006357;GO:0007010;GO:0007219;GO:0007417;GO:0007507;GO:0008285;GO:0010564;GO:0010628;GO:0010629;GO:0014032;GO:0014036;GO:0019100;GO:0030154;GO:0030155;GO:0030198;GO:0030238;GO:0030502;GO:0030857;GO:0030858;GO:0030879;GO:0030903;GO:0031018;GO:0032808;GO:0034504;GO:0035622;GO:0043491;GO:0045165;GO:0045595;GO:0045732;GO:0046533;GO:0048709;GO:0048873;GO:0060009;GO:0060018;GO:0060174;GO:0060221;GO:0060350;GO:0060441;GO:0060487;GO:0060512;GO:0060517;GO:0060532;GO:0060534;GO:0060729;GO:0060784;GO:0061046;GO:0061138;GO:0061145;GO:0070384;GO:0071599;GO:0071773;GO:0072034;GO:0072170;GO:0072189;GO:0072190;GO:0072193;GO:0072197;GO:0072289;GO:0097065;GO:0098609;GO:1901203;GO:2000138;GO:2000794;GO:2001054;GO:0005667;GO:0044798;
14020 ;GO:0005737;GO:0005634;GO:0016020;GO:0000166;GO:0005524;GO:0005829;GO:0016740;GO:0016301;GO:0016310;GO:0005886;GO:0008152;GO:0003951;GO:0005515;GO:0000287;GO:0046834;GO:0003376;GO:0005516;GO:0010800;GO:0030335;GO:0031398;GO:0006457;GO:0043066;GO:0007165;GO:0051092;GO:1901224;GO:0003677;GO:0030307;GO:0035556;GO:0030148;GO:0045766;GO:0019722;GO:0045931;GO:0038036;GO:0045987;GO:0048146;GO:0046512;GO:0051721;GO:0010803;GO:0006670;GO:0008481;GO:0017050;GO:0046521;GO:0001568;GO:0006954;GO:0007420;GO:0008284;GO:0032651
2562 GO:0006629;GO:0005576;GO:0004465;GO:0052689;;GO:0005615;GO:0042632;GO:0016787;GO:0016042;GO:0004620;GO:0007584;GO:0008283;GO:0050746;GO:0004806;GO:0008201;GO:0005794;GO:0005769;GO:0034375;GO:0043691;GO:0055091;GO:0009395;GO:0009986;GO:0032376;GO:0008970;GO:0010983
2157 GO:0005634;GO:0005737;GO:0005829;GO:0006351;GO:0006355;GO:0045893;GO:0008289;GO:0070062;GO:0005543;GO:0008431;GO:0045540;
2248 GO:0046872;GO:0055114;GO:0004392;GO:0006788;GO:0005615;GO:0045766;GO:1903589;GO:0090050;GO:0005634;GO:0016020;GO:0016021;GO:0016491;GO:0005783;GO:0005789;GO:0042803;GO:0005515;GO:0006915;GO:0048471;GO:0043231;GO:0051260;GO:0043123;GO:0019221;GO:0019899;GO:0001525;GO:0048661;GO:0071456;GO:1902042;GO:0020037;GO:0034605;GO:0045765;GO:0008219;GO:0035556;GO:0048662;GO:0006979;GO:0006879;GO:0042542;GO:0034383;GO:0045080;GO:0055072;GO:0004630;GO:0001666;GO:0001935;GO:0002246;GO:0002686;GO:0007264;GO:0007588;GO:0008217;GO:0008285;GO:0008630;GO:0010656;GO:0014806;GO:0016239;GO:0016242;GO:0031670;GO:0032764;GO:0034101;GO:0034395;GO:0035094;GO:0042167;GO:0042168;GO:0043065;GO:0043305;GO:0043392;GO:0043433;GO:0043524;GO:0043619;GO:0043627;GO:0051090;GO:0071243;GO:0071276;GO:0072719;GO:0097421;GO:0097755;GO:1904036;GO:1904706;GO:0005730;GO:0005829;GO:0005901;
go_descrip
31021
4381 membrane;integral component of membrane;centrosome;nucleoplasm;cell adhesion;cell junction;protein binding;integrin-mediated signaling pathway;cell-matrix adhesion;integrin complex;viral entry into host cell;focal adhesion;plasma membrane;signaling receptor activity;integrin binding;viral process;extracellular matrix organization;virus receptor activity;receptor complex;cell adhesion mediated by integrin;inflammatory response;transforming growth factor-beta secretion;external side of plasma membrane;integrin alphav-beta6 complex;
7662 ;cytoplasm;transferase activity;protein ubiquitination;ubiquitin-protein transferase activity;cytoskeleton;spindle;ubiquitin protein ligase activity;proteasome-mediated ubiquitin-dependent protein catabolic process;protein binding;mitotic spindle;regulation of mitotic metaphase/anaphase transition
28538
8570 nucleus;protein dimerization activity;regulation of transcription, DNA-templated;transcription, DNA-templated;DNA binding;multicellular organism development;cell differentiation;RNA polymerase II transcription factor activity, sequence-specific DNA binding;transcription factor binding;DNA binding transcription factor activity;transcription cofactor activity;transcription factor complex;RNA polymerase II regulatory region sequence-specific DNA binding;transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding;negative regulation of transcription by RNA polymerase II;regulation of transcription by RNA polymerase II;nervous system development;
5175 ;membrane;integral component of membrane;plasma membrane;molecular_function;biological_process;sarcolemma;regulation of heart rate
934 aorta smooth muscle tissue morphogenesis;cytoplasm;metal ion binding;nucleotide binding;protein kinase activity;protein serine/threonine kinase activity;ATP binding;protein phosphorylation;transferase activity;kinase activity;phosphorylation;actin binding;plasma membrane;actin cytoskeleton;cytoskeleton;cell projection;protein binding;calmodulin binding;cytosol;lamellipodium;stress fiber;cleavage furrow;positive regulation of cell migration;positive regulation of calcium ion transport;muscle contraction;smooth muscle contraction;positive regulation of wound healing;bleb assembly;myosin light chain kinase activity;tonic smooth muscle contraction;cellular hypotonic response;
13727 membrane;integral component of membrane;transporter activity;transmembrane transport;plasma membrane;sodium-independent organic anion transmembrane transporter activity;sodium-independent organic anion transport;integral component of plasma membrane;lipid transporter activity;lipid transport;prostaglandin transmembrane transporter activity;prostaglandin transport;ion transport;
15279 intracellular;membrane;integral component of membrane;growth factor activity;extracellular region;extracellular space;positive regulation of cell proliferation;integral component of plasma membrane;epidermal growth factor receptor binding;angiogenesis;positive regulation of MAP kinase activity;positive regulation of epidermal growth factor-activated receptor activity;positive regulation of mitotic nuclear division;activation of MAPK activity;positive regulation of epithelial cell proliferation;MAPK cascade;
4845 ;Golgi apparatus;cell adhesion;ossification;extracellular space;embryo implantation;regulation of signaling receptor activity;protein binding;extracellular region;cytokine activity;extracellular exosome;biomineral tissue development;cellular protein metabolic process;post-translational protein modification;endoplasmic reticulum lumen;extracellular matrix organization;positive regulation of transcription, DNA-templated;response to vitamin D;decidualization;extracellular matrix binding;osteoblast differentiation;androgen catabolic process;positive regulation of bone resorption;cellular response to testosterone stimulus;positive regulation of estradiol secretion;inflammatory response;response to organic substance;cell differentiation;response to steroid hormone;negative regulation of collateral sprouting of intact axon in response to injury;vesicle;cell projection;perinuclear region of cytoplasm
14924 metal ion binding;hydrolase activity;catalytic activity;metabolic process;extracellular region;sulfuric ester hydrolase activity;endoplasmic reticulum lumen;arylsulfatase activity;membrane;integral component of membrane;
13297 nucleus;cytoplasm;intracellular membrane-bounded organelle;cytosol;actin binding;actin cytoskeleton;cytoskeleton;protein binding;cell junction;focal adhesion;stress fiber;Z disc;14-3-3 protein binding;filamin binding;positive regulation of actin filament bundle assembly;alpha-actinin binding;muscle alpha-actinin binding;autophagosome assembly;tethering complex;
9313 membrane;growth factor activity;regulation of signaling receptor activity;protein binding;extracellular region;multicellular organism development;cell differentiation;extracellular space;positive chemotaxis;angiogenesis;positive regulation of cell division;signal transduction;platelet degranulation;platelet alpha granule lumen;vascular endothelial growth factor receptor signaling pathway;chemoattractant activity;vascular endothelial growth factor receptor 3 binding;response to hypoxia;positive regulation of endothelial cell proliferation;positive regulation of cell proliferation;positive regulation of angiogenesis;induction of positive chemotaxis;positive regulation of mast cell chemotaxis;positive regulation of cell-matrix adhesion;positive regulation of neuroblast proliferation;substrate-dependent cell migration;negative regulation of cell proliferation;animal organ morphogenesis;morphogenesis of embryonic epithelium;regulation of vascular endothelial growth factor receptor signaling pathway;positive regulation of protein autophosphorylation;response to drug;positive regulation of blood vessel endothelial cell migration;negative regulation of blood pressure;positive regulation of epithelial cell proliferation;positive regulation of protein secretion;positive regulation of peptidyl-tyrosine phosphorylation;positive regulation of lymphangiogenesis;cellular response to leukemia inhibitory factor;
11274 membrane;integral component of membrane;metal ion binding;cell adhesion;protein binding;cell surface;integrin-mediated signaling pathway;integrin complex;viral entry into host cell;focal adhesion;plasma membrane;viral process;blood coagulation;extracellular matrix organization;protein-containing complex binding;animal organ morphogenesis;cell-matrix adhesion;virus receptor activity;collagen binding;cell-substrate adhesion;amyloid-beta binding;collagen binding involved in cell-matrix adhesion;cell adhesion mediated by integrin;mesodermal cell differentiation;integrin alpha2-beta1 complex;integrin binding;collagen receptor activity;laminin binding;heparan sulfate proteoglycan binding;protein heterodimerization activity;response to hypoxia;positive regulation of leukocyte migration;substrate-dependent cell migration;hypotonic response;female pregnancy;cell proliferation;positive regulation of epithelial cell migration;positive regulation of alkaline phosphatase activity;response to organic cyclic compound;response to amine;response to muscle activity;positive regulation of smooth muscle cell migration;mammary gland development;positive regulation of cell projection organization;positive regulation of collagen biosynthetic process;positive regulation of collagen binding;response to L-ascorbic acid;collagen-activated signaling pathway;wound healing;response to drug;positive regulation of DNA binding;skin morphogenesis;establishment of protein localization;positive regulation of translation;positive regulation of cell adhesion;positive regulation of smooth muscle contraction;focal adhesion assembly;positive regulation of smooth muscle cell proliferation;positive regulation of inflammatory response;positive regulation of positive chemotaxis;detection of mechanical stimulus involved in sensory perception of pain;positive regulation of transmission of nerve impulse;positive regulation of phagocytosis, engulfment;hepatocyte differentiation;response to parathyroid hormone;cellular response to mechanical stimulus;cellular response to estradiol stimulus;cellular response to organic cyclic compound;nucleus;external side of plasma membrane;axon;cell projection;axon terminus;basal part of cell;perinuclear region of cytoplasm;
13332 membrane;integral component of membrane;ion transport;magnesium ion transmembrane transporter activity;magnesium ion transport;magnesium ion transmembrane transport;
7450 positive regulation of transcription by RNA polymerase II;nucleus;regulation of transcription, DNA-templated;transcription by RNA polymerase II;transcription, DNA-templated;DNA binding;chromosome;chromatin;protein binding;ligand-dependent nuclear receptor transcription coactivator activity;positive regulation of transcription, DNA-templated;focal adhesion;DNA binding transcription factor activity;RNA polymerase II transcription factor activity, sequence-specific DNA binding;nucleoplasm;cytosol;negative regulation of cell proliferation;retinoic acid receptor binding;response to virus;base-excision repair;enzyme binding;transcription factor binding;negative regulation of transcription, DNA-templated;chromatin binding;RNA polymerase II transcription factor complex;protein-containing complex assembly;transcription factor complex;nuclear transport;establishment of integrated proviral latency;transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific DNA binding;nucleosome disassembly;retinoid X receptor binding;enhancer sequence-specific DNA binding;AT DNA binding;DNA-(apurinic or apyrimidinic site) endonuclease activity;peroxisome proliferator activated receptor binding;DNA unwinding involved in DNA replication;negative regulation of chromatin silencing;senescence-associated heterochromatin focus assembly;oncogene-induced cell senescence;positive regulation of cellular senescence;senescence-associated heterochromatin focus;
28168
3341 calcium ion binding;metal ion binding;myosin complex;protein binding;cytosol;muscle contraction;A band;dendritic spine;
10067 membrane;integral component of membrane;nucleoplasm;signaling receptor binding;embryo development;nucleus;peptidyl-tyrosine phosphorylation;growth factor activity;regulation of signaling receptor activity;plasma membrane;extracellular region;extracellular space;phosphatidylinositol phosphorylation;activation of transmembrane receptor protein tyrosine kinase activity;positive regulation of cell proliferation;integrin binding;phosphatidylinositol-4,5-bisphosphate 3-kinase activity;positive regulation of protein kinase B signaling;Ras guanyl-nucleotide exchange factor activity;MAPK cascade;receptor tyrosine kinase binding;cytokine activity;protein tyrosine kinase activity;ERBB2 signaling pathway;regulation of cell motility;activation of protein kinase B activity;cardiac muscle cell differentiation;activation of MAPK activity;neural crest cell development;negative regulation of transcription, DNA-templated;nervous system development;transmembrane receptor protein tyrosine kinase activator activity;negative regulation of secretion;wound healing;mammary gland development;ventricular trabecula myocardium morphogenesis;ErbB-3 class receptor binding;ERBB signaling pathway;endocardial cell differentiation;transcription cofactor activity;cell communication;ERBB3 signaling pathway;regulation of protein homodimerization activity;regulation of protein heterodimerization activity;positive regulation of striated muscle cell differentiation;cardiac muscle cell myoblast differentiation;;ErbB-2 class receptor binding;chemorepellent activity;cell morphogenesis;startle response;cardiac conduction system development;synapse assembly;brain development;peripheral nervous system development;heart development;muscle organ development;locomotory behavior;axon ensheathment;glial cell differentiation;positive regulation of gene expression;positive regulation of phosphatidylinositol 3-kinase signaling;cell migration;glial cell fate commitment;chemorepulsion involved in interneuron migration from the subpallium to the cortex;neurogenesis;positive regulation of myelination;negative regulation of protein catabolic process;neurotransmitter receptor metabolic process;regulation of cell differentiation;positive regulation of axon extension;positive regulation of protein kinase activity;positive regulation of transcription by RNA polymerase II;positive regulation of Ras protein signal transduction;neuron fate commitment;oligodendrocyte differentiation;cardiac muscle tissue development;positive regulation of peptidyl-tyrosine phosphorylation;positive regulation of dendritic spine development;positive regulation of calcineurin-NFAT signaling cascade;positive regulation of protein localization to cell surface;positive regulation of cardiac muscle cell differentiation;negative regulation of neuron migration;cytoplasm;integral component of plasma membrane;external side of plasma membrane;axon;dendrite;axolemma;neuromuscular junction;cell body;synapse
2910 cytoplasm;hydrolase activity;extracellular region;peptidase activity;proteolysis;serine-type endopeptidase activity;extracellular space;serine-type peptidase activity;protein binding;extracellular exosome;signaling receptor binding;plasminogen activation;cell surface;blood coagulation;cellular protein modification process;negative regulation of proteolysis;fibrinolysis;phosphoprotein binding;response to hypoxia;smooth muscle cell migration;platelet-derived growth factor receptor signaling pathway;secretory granule;apical part of cell;
457 positive regulation of ERK1 and ERK2 cascade;membrane;integral component of membrane;Golgi apparatus;cytosol;cell adhesion;plasma membrane;cell projection;protein binding;hyaluronic acid binding;cartilage development;cell surface;focal adhesion;leukocyte migration;integral component of plasma membrane;extracellular exosome;microvillus;neutrophil degranulation;apical plasma membrane;positive regulation of peptidyl-serine phosphorylation;negative regulation of apoptotic process;positive regulation of peptidyl-tyrosine phosphorylation;secretory granule membrane;extracellular matrix organization;negative regulation of cysteine-type endopeptidase activity involved in apoptotic process;interferon-gamma-mediated signaling pathway;wound healing, spreading of cells;extracellular matrix disassembly;cell-matrix adhesion;cellular response to fibroblast growth factor stimulus;cell-cell adhesion;collagen binding;negative regulation of DNA damage response, signal transduction by p53 class mediator;negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator;hyaluronan catabolic process;positive regulation of heterotypic cell-cell adhesion;monocyte aggregation;cytokine receptor activity;macrophage migration inhibitory factor receptor complex;lamellipodium membrane;regulation of lamellipodium morphogenesis;positive regulation of monocyte aggregation;;positive regulation of kinase activity
46915
3449 cytoplasm;nucleotide binding;ATP binding;cytoskeleton;extracellular space;cytosol;extracellular exosome;response to virus;protein-containing complex;positive regulation of gene expression;protein kinase binding;regulation of blood pressure;vascular smooth muscle contraction;mesenchyme migration;actin cytoskeleton;lamellipodium;filopodium;smooth muscle contractile fiber;cell body;muscle contraction;glomerular mesangial cell development;
7759 membrane;integral component of membrane;transferase activity;Golgi apparatus;acetylgalactosaminyltransferase activity;Golgi cisterna membrane;N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase activity;
3928 magnesium ion binding;glycolytic process;phosphopyruvate hydratase activity;phosphopyruvate hydratase complex;cytoplasm;membrane;metal ion binding;cytosol;lyase activity;plasma membrane;protein binding;extracellular space;extracellular exosome;gluconeogenesis;canonical glycolysis;;photoreceptor inner segment;intracellular;neuronal cell body;perikaryon;myelin sheath
11706 nucleus;nuclear speck;regulation of transcription, DNA-templated;transcription, DNA-templated;DNA binding;DNA binding transcription factor activity;protein binding;cellular response to DNA damage stimulus;cell cycle;transcription factor complex;identical protein binding;negative regulation of transcription by RNA polymerase II;RNA polymerase II transcription factor activity, sequence-specific DNA binding;proximal promoter sequence-specific DNA binding;transcription corepressor activity;placenta development;sprouting angiogenesis;DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest;negative regulation of cytokinesis;positive regulation of DNA endoreduplication;positive regulation of transcription by RNA polymerase II;trophoblast giant cell differentiation;chorionic trophoblast cell differentiation;hepatocyte differentiation;nucleoplasm;negative regulation of cell proliferation;DNA damage response, signal transduction by p53 class mediator;negative regulation of G1/S transition of mitotic cell cycle;RNA polymerase II regulatory region sequence-specific DNA binding;RNA polymerase II proximal promoter sequence-specific DNA binding;transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding;negative regulation of transcription involved in G1/S transition of mitotic cell cycle;
10325 cytoplasm;membrane;nucleotide binding;ATP binding;cytoskeleton;extracellular space;focal adhesion;cytosol;extracellular exosome;ATPase activity;blood microparticle;I band;positive regulation of gene expression;mesenchyme migration;lamellipodium;filopodium;cell body;actin filament;myosin binding;muscle filament sliding;sarcomere;actin filament-based movement;heart contraction;cardiac myofibril assembly;cardiac muscle tissue morphogenesis;actomyosin structure organization;skeletal muscle thin filament assembly;actin-myosin filament sliding;response to drug;negative regulation of apoptotic process;response to ethanol;cardiac muscle contraction;actin-mediated cell contraction;actomyosin, actin portion;
81 nucleus;cytoplasm;regulation of transcription, DNA-templated;transcription by RNA polymerase II;transcription, DNA-templated;DNA binding;DNA binding transcription factor activity;sequence-specific DNA binding;protein binding;multicellular organism development;RNA polymerase II transcription factor activity, sequence-specific DNA binding;positive regulation of transcription by RNA polymerase II;molecular_function;biological_process;transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding;somite development;RNA polymerase II proximal promoter sequence-specific DNA binding;chromatin binding;HMG box domain binding;somite specification;hematopoietic stem cell differentiation;sclerotome development
5642 positive regulation of transcription by RNA polymerase II;nucleus;nucleoplasm;regulation of transcription, DNA-templated;transcription, DNA-templated;DNA binding;DNA binding transcription factor activity;nucleosome assembly;RNA polymerase II transcription factor activity, sequence-specific DNA binding;protein binding;signal transduction;cartilage development;positive regulation of transcription, DNA-templated;spermatogenesis;positive regulation of protein phosphorylation;skeletal system development;negative regulation of canonical Wnt signaling pathway;chromatin binding;male gonad development;protein-containing complex;cellular response to interleukin-1;cellular response to mechanical stimulus;positive regulation of cell proliferation;regulation of cell proliferation;regulation of apoptotic process;negative regulation of apoptotic process;ERK1 and ERK2 cascade;cartilage condensation;positive regulation of chondrocyte differentiation;negative regulation of transcription, DNA-templated;transcription initiation from RNA polymerase II promoter;protein kinase activity;cAMP-mediated signaling;cellular response to transforming growth factor beta stimulus;epidermal growth factor receptor signaling pathway;negative regulation of myoblast differentiation;retina development in camera-type eye;RNA polymerase II proximal promoter sequence-specific DNA binding;chondrocyte differentiation;negative regulation of pri-miRNA transcription by RNA polymerase II;cellular response to retinoic acid;protein-containing complex assembly;negative regulation of epithelial cell proliferation;chromatin remodeling;enhancer sequence-specific DNA binding;transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding;prostate gland development;positive regulation of epithelial cell migration;positive regulation of epithelial cell proliferation;positive regulation of phosphatidylinositol 3-kinase signaling;protein kinase A catalytic subunit binding;cell fate specification;positive regulation of mesenchymal cell proliferation;cochlea morphogenesis;Sertoli cell differentiation;tissue homeostasis;cellular response to epidermal growth factor stimulus;negative regulation of ossification;negative regulation of biomineral tissue development;cellular response to heparin;transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding;epithelial to mesenchymal transition;positive regulation of branching involved in ureteric bud morphogenesis;hair follicle development;endocardial cushion morphogenesis;positive regulation of kidney development;positive regulation of male gonad development;otic vesicle formation;positive regulation of cartilage development;positive regulation of mesenchymal stem cell differentiation;negative regulation of chondrocyte differentiation;somatic stem cell population maintenance;transcription regulatory region sequence-specific DNA binding;transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding;beta-catenin binding;enhancer binding;bHLH transcription factor binding;sequence-specific DNA binding;transcription regulatory region DNA binding;protein heterodimerization activity;pre-mRNA intronic binding;negative regulation of transcription by RNA polymerase II;ossification;branching involved in ureteric bud morphogenesis;chondrocyte development;negative regulation of immune system process;heart valve development;heart valve morphogenesis;heart valve formation;chondrocyte differentiation involved in endochondral bone morphogenesis;chondrocyte hypertrophy;regulation of transcription by RNA polymerase II;cytoskeleton organization;Notch signaling pathway;central nervous system development;heart development;negative regulation of cell proliferation;regulation of cell cycle process;positive regulation of gene expression;negative regulation of gene expression;neural crest cell development;neural crest cell fate specification;male germ-line sex determination;cell differentiation;regulation of cell adhesion;extracellular matrix organization;male sex determination;negative regulation of bone mineralization;negative regulation of epithelial cell differentiation;positive regulation of epithelial cell differentiation;mammary gland development;notochord development;endocrine pancreas development;lacrimal gland development;protein localization to nucleus;intrahepatic bile duct development;protein kinase B signaling;cell fate commitment;regulation of cell differentiation;positive regulation of protein catabolic process;negative regulation of photoreceptor cell differentiation;oligodendrocyte differentiation;homeostasis of number of cells within a tissue;Sertoli cell development;astrocyte fate commitment;limb bud formation;retinal rod cell differentiation;endochondral bone morphogenesis;epithelial tube branching involved in lung morphogenesis;lung epithelial cell differentiation;prostate gland morphogenesis;epithelial cell proliferation involved in prostatic bud elongation;bronchus cartilage development;trachea cartilage development;intestinal epithelial structure maintenance;regulation of cell proliferation involved in tissue homeostasis;regulation of branching involved in lung morphogenesis;morphogenesis of a branching epithelium;lung smooth muscle development;Harderian gland development;otic vesicle development;cellular response to BMP stimulus;renal vesicle induction;metanephric tubule development;ureter development;ureter urothelium development;ureter smooth muscle cell differentiation;ureter morphogenesis;metanephric nephron tubule formation;anterior head development;cell-cell adhesion;positive regulation of extracellular matrix assembly;positive regulation of cell proliferation involved in heart morphogenesis;regulation of epithelial cell proliferation involved in lung morphogenesis;negative regulation of mesenchymal cell apoptotic process;transcription factor complex;nuclear transcription factor complex;
14020 ;cytoplasm;nucleus;membrane;nucleotide binding;ATP binding;cytosol;transferase activity;kinase activity;phosphorylation;plasma membrane;metabolic process;NAD+ kinase activity;protein binding;magnesium ion binding;lipid phosphorylation;sphingosine-1-phosphate signaling pathway;calmodulin binding;positive regulation of peptidyl-threonine phosphorylation;positive regulation of cell migration;positive regulation of protein ubiquitination;protein folding;negative regulation of apoptotic process;signal transduction;positive regulation of NF-kappaB transcription factor activity;positive regulation of NIK/NF-kappaB signaling;DNA binding;positive regulation of cell growth;intracellular signal transduction;sphingolipid biosynthetic process;positive regulation of angiogenesis;calcium-mediated signaling;positive regulation of mitotic cell cycle;sphingosine-1-phosphate receptor activity;positive regulation of smooth muscle contraction;positive regulation of fibroblast proliferation;sphingosine biosynthetic process;protein phosphatase 2A binding;regulation of tumor necrosis factor-mediated signaling pathway;sphingosine metabolic process;sphinganine kinase activity;D-erythro-sphingosine kinase activity;sphingoid catabolic process;blood vessel development;inflammatory response;brain development;positive regulation of cell proliferation;regulation of interleukin-1 beta production
2562 lipid metabolic process;extracellular region;lipoprotein lipase activity;carboxylic ester hydrolase activity;;extracellular space;cholesterol homeostasis;hydrolase activity;lipid catabolic process;phospholipase activity;response to nutrient;cell proliferation;regulation of lipoprotein metabolic process;triglyceride lipase activity;heparin binding;Golgi apparatus;early endosome;high-density lipoprotein particle remodeling;reverse cholesterol transport;phospholipid homeostasis;phospholipid catabolic process;cell surface;positive regulation of cholesterol transport;phosphatidylcholine 1-acylhydrolase activity;positive regulation of high-density lipoprotein particle clearance
2157 nucleus;cytoplasm;cytosol;transcription, DNA-templated;regulation of transcription, DNA-templated;positive regulation of transcription, DNA-templated;lipid binding;extracellular exosome;phospholipid binding;vitamin E binding;regulation of cholesterol biosynthetic process;
2248 metal ion binding;oxidation-reduction process;heme oxygenase (decyclizing) activity;heme oxidation;extracellular space;positive regulation of angiogenesis;positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis;positive regulation of cell migration involved in sprouting angiogenesis;nucleus;membrane;integral component of membrane;oxidoreductase activity;endoplasmic reticulum;endoplasmic reticulum membrane;protein homodimerization activity;protein binding;apoptotic process;perinuclear region of cytoplasm;intracellular membrane-bounded organelle;protein homooligomerization;positive regulation of I-kappaB kinase/NF-kappaB signaling;cytokine-mediated signaling pathway;enzyme binding;angiogenesis;positive regulation of smooth muscle cell proliferation;cellular response to hypoxia;negative regulation of extrinsic apoptotic signaling pathway via death domain receptors;heme binding;cellular response to heat;regulation of angiogenesis;cell death;intracellular signal transduction;negative regulation of smooth muscle cell proliferation;response to oxidative stress;cellular iron ion homeostasis;response to hydrogen peroxide;low-density lipoprotein particle clearance;positive regulation of chemokine biosynthetic process;iron ion homeostasis;phospholipase D activity;response to hypoxia;endothelial cell proliferation;wound healing involved in inflammatory response;negative regulation of leukocyte migration;small GTPase mediated signal transduction;excretion;regulation of blood pressure;negative regulation of cell proliferation;intrinsic apoptotic signaling pathway in response to DNA damage;negative regulation of muscle cell apoptotic process;smooth muscle hyperplasia;positive regulation of macroautophagy;negative regulation of macroautophagy;cellular response to nutrient;negative regulation of mast cell cytokine production;erythrocyte homeostasis;regulation of transcription from RNA polymerase II promoter in response to iron;response to nicotine;heme catabolic process;heme metabolic process;positive regulation of apoptotic process;negative regulation of mast cell degranulation;negative regulation of DNA binding;negative regulation of DNA binding transcription factor activity;negative regulation of neuron apoptotic process;regulation of transcription from RNA polymerase II promoter in response to oxidative stress;response to estrogen;regulation of DNA binding transcription factor activity;cellular response to arsenic-containing substance;cellular response to cadmium ion;cellular response to cisplatin;liver regeneration;positive regulation of blood vessel diameter;negative regulation of epithelial cell apoptotic process;negative regulation of vascular smooth muscle cell proliferation;nucleolus;cytosol;caveola;
#separate into up and downregulated
# upregulated
#logFC > 1
significant_genes_05_FC1_up <- row.names(results[["Unstrain"]])[results[["Unstrain"]]$qvalue < 0.05 & results[["Unstrain"]]$logFC > 1]
significant_symbols_05_FC1_up <- gene_anno$external_gene_name[match(significant_genes_05_FC1_up, gene_anno$ensembl_gene_id)]
significant_anno_05_FC1_up <- gene_anno[match(significant_genes_05_FC1_up, gene_anno$ensembl_gene_id),]
significant_anno_05_FC1_up
ensembl_gene_id external_gene_name chromosome_name
5175 ENSG00000121577 POPDC2 3
934 ENSG00000065534 MYLK 3
15279 ENSG00000182585 EPGN 4
14924 ENSG00000180801 ARSJ 4
13297 ENSG00000172403 SYNPO2 4
13332 ENSG00000172548 NIPAL4 5
28168 ENSG00000228495 LINC01013 6
3341 ENSG00000106631 MYL7 7
10067 ENSG00000157168 NRG1 8
3449 ENSG00000107796 ACTA2 10
10325 ENSG00000159251 ACTC1 15
81 ENSG00000005102 MEOX1 17
description
5175 popeye domain containing 2 [Source:HGNC Symbol;Acc:HGNC:17648]
934 myosin light chain kinase [Source:HGNC Symbol;Acc:HGNC:7590]
15279 epithelial mitogen [Source:HGNC Symbol;Acc:HGNC:17470]
14924 arylsulfatase family member J [Source:HGNC Symbol;Acc:HGNC:26286]
13297 synaptopodin 2 [Source:HGNC Symbol;Acc:HGNC:17732]
13332 NIPA like domain containing 4 [Source:HGNC Symbol;Acc:HGNC:28018]
28168 long intergenic non-protein coding RNA 1013 [Source:HGNC Symbol;Acc:HGNC:48987]
3341 myosin light chain 7 [Source:HGNC Symbol;Acc:HGNC:21719]
10067 neuregulin 1 [Source:HGNC Symbol;Acc:HGNC:7997]
3449 actin, alpha 2, smooth muscle, aorta [Source:HGNC Symbol;Acc:HGNC:130]
10325 actin, alpha, cardiac muscle 1 [Source:HGNC Symbol;Acc:HGNC:143]
81 mesenchyme homeobox 1 [Source:HGNC Symbol;Acc:HGNC:7013]
start_position end_position
5175 119636457 119665324
934 123610049 123884331
15279 74308473 74315307
14924 113900284 113979727
13297 118850688 119061247
13332 157460019 157474717
28168 132131915 132169374
3341 44138864 44141332
10067 31639386 32767959
3449 88935074 88991339
10325 34788096 34796139
81 43640388 43661954
phenotype
5175
934 Megacystis-microcolon-intestinal hypoperistalsis syndrome;Familial thoracic aortic aneurysm and aortic dissection;AORTIC ANEURYSM FAMILIAL THORACIC 7
15279
14924
13297
13332 Congenital nonbullous ichthyosiform erythroderma;Lamellar ichthyosis;ICHTHYOSIS CONGENITAL AUTOSOMAL RECESSIVE 6
28168
3341
10067 SCHIZOPHRENIA 6
3449 AORTIC ANEURYSM FAMILIAL THORACIC 6;Moyamoya disease 5;Familial thoracic aortic aneurysm and aortic dissection;Moyamoya disease;MULTISYSTEMIC SMOOTH MUSCLE DYSFUNCTION SYNDROME
10325 Atrial septal defect ostium secundum type;Familial isolated dilated cardiomyopathy;Left ventricular noncompaction;ATRIAL SEPTAL DEFECT 5;Cardiomyopathy dilated 1R;CARDIOMYOPATHY FAMILIAL HYPERTROPHIC 11
81 Klippel-Feil Anomaly;Isolated Klippel-Feil syndrome;KLIPPEL-FEIL SYNDROME 2 AUTOSOMAL RECESSIVE
go_id
5175 ;GO:0016020;GO:0016021;GO:0005886;GO:0003674;GO:0008150;GO:0042383;GO:0002027
934 GO:0060414;GO:0005737;GO:0046872;GO:0000166;GO:0004672;GO:0004674;GO:0005524;GO:0006468;GO:0016740;GO:0016301;GO:0016310;GO:0003779;GO:0005886;GO:0015629;GO:0005856;GO:0042995;GO:0005515;GO:0005516;GO:0005829;GO:0030027;GO:0001725;GO:0032154;GO:0030335;GO:0051928;GO:0006936;GO:0006939;GO:0090303;GO:0032060;GO:0004687;GO:0014820;GO:0071476;
15279 GO:0005622;GO:0016020;GO:0016021;GO:0008083;GO:0005576;GO:0005615;GO:0008284;GO:0005887;GO:0005154;GO:0001525;GO:0043406;GO:0045741;GO:0045840;GO:0000187;GO:0050679;GO:0000165;
14924 GO:0046872;GO:0016787;GO:0003824;GO:0008152;GO:0005576;GO:0008484;GO:0005788;GO:0004065;GO:0016020;GO:0016021;
13297 GO:0005634;GO:0005737;GO:0043231;GO:0005829;GO:0003779;GO:0015629;GO:0005856;GO:0005515;GO:0030054;GO:0005925;GO:0001725;GO:0030018;GO:0071889;GO:0031005;GO:0032233;GO:0051393;GO:0051371;GO:0000045;GO:0099023;
13332 GO:0016020;GO:0016021;GO:0006811;GO:0015095;GO:0015693;GO:1903830;
28168
3341 GO:0005509;GO:0046872;GO:0016459;GO:0005515;GO:0005829;GO:0006936;GO:0031672;GO:0043197;
10067 GO:0016020;GO:0016021;GO:0005654;GO:0005102;GO:0009790;GO:0005634;GO:0018108;GO:0008083;GO:0010469;GO:0005886;GO:0005576;GO:0005615;GO:0046854;GO:0007171;GO:0008284;GO:0005178;GO:0046934;GO:0051897;GO:0005088;GO:0000165;GO:0030971;GO:0005125;GO:0004713;GO:0038128;GO:2000145;GO:0032148;GO:0055007;GO:0000187;GO:0014032;GO:0045892;GO:0007399;GO:0030297;GO:0051048;GO:0042060;GO:0030879;GO:0003222;GO:0043125;GO:0038127;GO:0060956;GO:0003712;GO:0007154;GO:0038129;GO:0043496;GO:0043497;GO:0051155;GO:0060379;;GO:0005176;GO:0045499;GO:0000902;GO:0001964;GO:0003161;GO:0007416;GO:0007420;GO:0007422;GO:0007507;GO:0007517;GO:0007626;GO:0008366;GO:0010001;GO:0010628;GO:0014068;GO:0016477;GO:0021781;GO:0021842;GO:0022008;GO:0031643;GO:0042177;GO:0045213;GO:0045595;GO:0045773;GO:0045860;GO:0045944;GO:0046579;GO:0048663;GO:0048709;GO:0048738;GO:0050731;GO:0060999;GO:0070886;GO:2000010;GO:2000727;GO:2001223;GO:0005737;GO:0005887;GO:0009897;GO:0030424;GO:0030425;GO:0030673;GO:0031594;GO:0044297;GO:0045202
3449 GO:0005737;GO:0000166;GO:0005524;GO:0005856;GO:0005615;GO:0005829;GO:0070062;GO:0009615;GO:0032991;GO:0010628;GO:0019901;GO:0008217;GO:0014829;GO:0090131;GO:0015629;GO:0030027;GO:0030175;GO:0030485;GO:0044297;GO:0006936;GO:0072144;
10325 GO:0005737;GO:0016020;GO:0000166;GO:0005524;GO:0005856;GO:0005615;GO:0005925;GO:0005829;GO:0070062;GO:0016887;GO:0072562;GO:0031674;GO:0010628;GO:0090131;GO:0030027;GO:0030175;GO:0044297;GO:0005884;GO:0017022;GO:0030049;GO:0030017;GO:0030048;GO:0060047;GO:0055003;GO:0055008;GO:0031032;GO:0030240;GO:0033275;GO:0042493;GO:0043066;GO:0045471;GO:0060048;GO:0070252;GO:0042643;
81 GO:0005634;GO:0005737;GO:0006355;GO:0006366;GO:0006351;GO:0003677;GO:0003700;GO:0043565;GO:0005515;GO:0007275;GO:0000981;GO:0045944;GO:0003674;GO:0008150;GO:0001077;GO:0061053;GO:0000978;GO:0003682;GO:0071837;GO:0001757;GO:0060218;GO:0061056
go_descrip
5175 ;membrane;integral component of membrane;plasma membrane;molecular_function;biological_process;sarcolemma;regulation of heart rate
934 aorta smooth muscle tissue morphogenesis;cytoplasm;metal ion binding;nucleotide binding;protein kinase activity;protein serine/threonine kinase activity;ATP binding;protein phosphorylation;transferase activity;kinase activity;phosphorylation;actin binding;plasma membrane;actin cytoskeleton;cytoskeleton;cell projection;protein binding;calmodulin binding;cytosol;lamellipodium;stress fiber;cleavage furrow;positive regulation of cell migration;positive regulation of calcium ion transport;muscle contraction;smooth muscle contraction;positive regulation of wound healing;bleb assembly;myosin light chain kinase activity;tonic smooth muscle contraction;cellular hypotonic response;
15279 intracellular;membrane;integral component of membrane;growth factor activity;extracellular region;extracellular space;positive regulation of cell proliferation;integral component of plasma membrane;epidermal growth factor receptor binding;angiogenesis;positive regulation of MAP kinase activity;positive regulation of epidermal growth factor-activated receptor activity;positive regulation of mitotic nuclear division;activation of MAPK activity;positive regulation of epithelial cell proliferation;MAPK cascade;
14924 metal ion binding;hydrolase activity;catalytic activity;metabolic process;extracellular region;sulfuric ester hydrolase activity;endoplasmic reticulum lumen;arylsulfatase activity;membrane;integral component of membrane;
13297 nucleus;cytoplasm;intracellular membrane-bounded organelle;cytosol;actin binding;actin cytoskeleton;cytoskeleton;protein binding;cell junction;focal adhesion;stress fiber;Z disc;14-3-3 protein binding;filamin binding;positive regulation of actin filament bundle assembly;alpha-actinin binding;muscle alpha-actinin binding;autophagosome assembly;tethering complex;
13332 membrane;integral component of membrane;ion transport;magnesium ion transmembrane transporter activity;magnesium ion transport;magnesium ion transmembrane transport;
28168
3341 calcium ion binding;metal ion binding;myosin complex;protein binding;cytosol;muscle contraction;A band;dendritic spine;
10067 membrane;integral component of membrane;nucleoplasm;signaling receptor binding;embryo development;nucleus;peptidyl-tyrosine phosphorylation;growth factor activity;regulation of signaling receptor activity;plasma membrane;extracellular region;extracellular space;phosphatidylinositol phosphorylation;activation of transmembrane receptor protein tyrosine kinase activity;positive regulation of cell proliferation;integrin binding;phosphatidylinositol-4,5-bisphosphate 3-kinase activity;positive regulation of protein kinase B signaling;Ras guanyl-nucleotide exchange factor activity;MAPK cascade;receptor tyrosine kinase binding;cytokine activity;protein tyrosine kinase activity;ERBB2 signaling pathway;regulation of cell motility;activation of protein kinase B activity;cardiac muscle cell differentiation;activation of MAPK activity;neural crest cell development;negative regulation of transcription, DNA-templated;nervous system development;transmembrane receptor protein tyrosine kinase activator activity;negative regulation of secretion;wound healing;mammary gland development;ventricular trabecula myocardium morphogenesis;ErbB-3 class receptor binding;ERBB signaling pathway;endocardial cell differentiation;transcription cofactor activity;cell communication;ERBB3 signaling pathway;regulation of protein homodimerization activity;regulation of protein heterodimerization activity;positive regulation of striated muscle cell differentiation;cardiac muscle cell myoblast differentiation;;ErbB-2 class receptor binding;chemorepellent activity;cell morphogenesis;startle response;cardiac conduction system development;synapse assembly;brain development;peripheral nervous system development;heart development;muscle organ development;locomotory behavior;axon ensheathment;glial cell differentiation;positive regulation of gene expression;positive regulation of phosphatidylinositol 3-kinase signaling;cell migration;glial cell fate commitment;chemorepulsion involved in interneuron migration from the subpallium to the cortex;neurogenesis;positive regulation of myelination;negative regulation of protein catabolic process;neurotransmitter receptor metabolic process;regulation of cell differentiation;positive regulation of axon extension;positive regulation of protein kinase activity;positive regulation of transcription by RNA polymerase II;positive regulation of Ras protein signal transduction;neuron fate commitment;oligodendrocyte differentiation;cardiac muscle tissue development;positive regulation of peptidyl-tyrosine phosphorylation;positive regulation of dendritic spine development;positive regulation of calcineurin-NFAT signaling cascade;positive regulation of protein localization to cell surface;positive regulation of cardiac muscle cell differentiation;negative regulation of neuron migration;cytoplasm;integral component of plasma membrane;external side of plasma membrane;axon;dendrite;axolemma;neuromuscular junction;cell body;synapse
3449 cytoplasm;nucleotide binding;ATP binding;cytoskeleton;extracellular space;cytosol;extracellular exosome;response to virus;protein-containing complex;positive regulation of gene expression;protein kinase binding;regulation of blood pressure;vascular smooth muscle contraction;mesenchyme migration;actin cytoskeleton;lamellipodium;filopodium;smooth muscle contractile fiber;cell body;muscle contraction;glomerular mesangial cell development;
10325 cytoplasm;membrane;nucleotide binding;ATP binding;cytoskeleton;extracellular space;focal adhesion;cytosol;extracellular exosome;ATPase activity;blood microparticle;I band;positive regulation of gene expression;mesenchyme migration;lamellipodium;filopodium;cell body;actin filament;myosin binding;muscle filament sliding;sarcomere;actin filament-based movement;heart contraction;cardiac myofibril assembly;cardiac muscle tissue morphogenesis;actomyosin structure organization;skeletal muscle thin filament assembly;actin-myosin filament sliding;response to drug;negative regulation of apoptotic process;response to ethanol;cardiac muscle contraction;actin-mediated cell contraction;actomyosin, actin portion;
81 nucleus;cytoplasm;regulation of transcription, DNA-templated;transcription by RNA polymerase II;transcription, DNA-templated;DNA binding;DNA binding transcription factor activity;sequence-specific DNA binding;protein binding;multicellular organism development;RNA polymerase II transcription factor activity, sequence-specific DNA binding;positive regulation of transcription by RNA polymerase II;molecular_function;biological_process;transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding;somite development;RNA polymerase II proximal promoter sequence-specific DNA binding;chromatin binding;HMG box domain binding;somite specification;hematopoietic stem cell differentiation;sclerotome development
# downregulated
#logFC < -1
significant_genes_05_FC1_down <- row.names(results[["Unstrain"]])[results[["Unstrain"]]$qvalue < 0.05 & results[["Unstrain"]]$logFC < -1]
significant_symbols_05_FC1_down <- gene_anno$external_gene_name[match(significant_genes_05_FC1_down, gene_anno$ensembl_gene_id)]
significant_anno_05_FC1_down <- gene_anno[match(significant_genes_05_FC1_down, gene_anno$ensembl_gene_id),]
significant_anno_05_FC1_down
ensembl_gene_id external_gene_name chromosome_name
31021 ENSG00000232679 LINC01705 1
4381 ENSG00000115221 ITGB6 2
7662 ENSG00000138411 HECW2 2
28538 ENSG00000229056 AC020571.1 2
8570 ENSG00000144485 HES6 2
13727 ENSG00000174640 SLCO2A1 3
4845 ENSG00000118785 SPP1 4
9313 ENSG00000150630 VEGFC 4
11274 ENSG00000164171 ITGA2 5
7450 ENSG00000137309 HMGA1 6
2910 ENSG00000104368 PLAT 8
457 ENSG00000026508 CD44 11
46915 ENSG00000261578 AP003119.3 11
7759 ENSG00000139044 B4GALNT3 12
3928 ENSG00000111674 ENO2 12
11706 ENSG00000165891 E2F7 12
5642 ENSG00000125398 SOX9 17
14020 ENSG00000176170 SPHK1 17
2562 ENSG00000101670 LIPG 18
2157 ENSG00000100003 SEC14L2 22
2248 ENSG00000100292 HMOX1 22
description
31021 long intergenic non-protein coding RNA 1705 [Source:HGNC Symbol;Acc:HGNC:52493]
4381 integrin subunit beta 6 [Source:HGNC Symbol;Acc:HGNC:6161]
7662 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:29853]
28538
8570 hes family bHLH transcription factor 6 [Source:HGNC Symbol;Acc:HGNC:18254]
13727 solute carrier organic anion transporter family member 2A1 [Source:HGNC Symbol;Acc:HGNC:10955]
4845 secreted phosphoprotein 1 [Source:HGNC Symbol;Acc:HGNC:11255]
9313 vascular endothelial growth factor C [Source:HGNC Symbol;Acc:HGNC:12682]
11274 integrin subunit alpha 2 [Source:HGNC Symbol;Acc:HGNC:6137]
7450 high mobility group AT-hook 1 [Source:HGNC Symbol;Acc:HGNC:5010]
2910 plasminogen activator, tissue type [Source:HGNC Symbol;Acc:HGNC:9051]
457 CD44 molecule (Indian blood group) [Source:HGNC Symbol;Acc:HGNC:1681]
46915
7759 beta-1,4-N-acetyl-galactosaminyltransferase 3 [Source:HGNC Symbol;Acc:HGNC:24137]
3928 enolase 2 [Source:HGNC Symbol;Acc:HGNC:3353]
11706 E2F transcription factor 7 [Source:HGNC Symbol;Acc:HGNC:23820]
5642 SRY-box 9 [Source:HGNC Symbol;Acc:HGNC:11204]
14020 sphingosine kinase 1 [Source:HGNC Symbol;Acc:HGNC:11240]
2562 lipase G, endothelial type [Source:HGNC Symbol;Acc:HGNC:6623]
2157 SEC14 like lipid binding 2 [Source:HGNC Symbol;Acc:HGNC:10699]
2248 heme oxygenase 1 [Source:HGNC Symbol;Acc:HGNC:5013]
start_position end_position
31021 222041705 222064763
4381 160099666 160200313
7662 196189099 196593692
28538 196260024 196264204
8570 238238267 238240662
13727 133932696 134052184
4845 87975650 87983426
9313 176683538 176792727
11274 52989326 53094779
7450 34236873 34246231
2910 42175233 42207724
457 35138870 35232402
46915 76800364 76804555
7759 460364 563509
3928 6913745 6923698
11706 77021247 77065580
5642 72121020 72126420
14020 76376584 76387860
2562 49560699 49599182
2157 30396857 30425317
2248 35380361 35394214
phenotype
31021
4381 Alopecia-intellectual disability syndrome;Hypocalcified amelogenesis imperfecta;Hypoplastic amelogenesis imperfecta;AMELOGENESIS IMPERFECTA TYPE IH
7662 HECW2;NEURODEVELOPMENTAL DISORDER WITH HYPOTONIA SEIZURES AND ABSENT LANGUAGE
28538
8570
13727 Chronic enteropathy associated with SLCO2A1 gene;Pachydermoperiostosis;HYPERTROPHIC OSTEOARTHROPATHY PRIMARY AUTOSOMAL RECESSIVE 2
4845 Pediatric systemic lupus erythematosus
9313 Milroy Disease;Lymphedema hereditary id
11274 Fetal and neonatal alloimmune thrombocytopenia;BLEEDING DISORDER PLATELET-TYPE 9
7450 DIABETES MELLITUS NONINSULIN-DEPENDENT
2910 Lethal hydranencephaly-diaphragmatic hernia syndrome;THROMBOPHILIA FAMILIAL DUE TO DECREASED RELEASE OF TISSUE PLASMINOGENACTIVATOR
457 BLOOD GROUP INDIAN SYSTEM
46915
7759
3928
11706
5642 CAMPOMELIC DYSPLASIA;PIERRE ROBIN SEQUENCE;46XX ovotesticular disorder of sex development;46XX testicular disorder of sex development;46XY complete gonadal dysgenesis;46XY partial gonadal dysgenesis;Isolated Pierre Robin syndrome;46XY SEX REVERSAL 10
14020
2562
2157
2248 HEME OXYGENASE 1 DEFICIENCY;PULMONARY DISEASE CHRONIC OBSTRUCTIVE
go_id
31021
4381 GO:0016020;GO:0016021;GO:0005813;GO:0005654;GO:0007155;GO:0030054;GO:0005515;GO:0007229;GO:0007160;GO:0008305;GO:0046718;GO:0005925;GO:0005886;GO:0038023;GO:0005178;GO:0016032;GO:0030198;GO:0001618;GO:0043235;GO:0033627;GO:0006954;GO:0038044;GO:0009897;GO:0034685;
7662 ;GO:0005737;GO:0016740;GO:0016567;GO:0004842;GO:0005856;GO:0005819;GO:0061630;GO:0043161;GO:0005515;GO:0072686;GO:0030071
28538
8570 GO:0005634;GO:0046983;GO:0006355;GO:0006351;GO:0003677;GO:0007275;GO:0030154;GO:0000981;GO:0008134;GO:0003700;GO:0003712;GO:0005667;GO:0000977;GO:0001227;GO:0000122;GO:0006357;GO:0007399;
13727 GO:0016020;GO:0016021;GO:0005215;GO:0055085;GO:0005886;GO:0015347;GO:0043252;GO:0005887;GO:0005319;GO:0006869;GO:0015132;GO:0015732;GO:0006811;
4845 ;GO:0005794;GO:0007155;GO:0001503;GO:0005615;GO:0007566;GO:0010469;GO:0005515;GO:0005576;GO:0005125;GO:0070062;GO:0031214;GO:0044267;GO:0043687;GO:0005788;GO:0030198;GO:0045893;GO:0033280;GO:0046697;GO:0050840;GO:0001649;GO:0006710;GO:0045780;GO:0071394;GO:2000866;GO:0006954;GO:0010033;GO:0030154;GO:0048545;GO:0048685;GO:0031982;GO:0042995;GO:0048471
9313 GO:0016020;GO:0008083;GO:0010469;GO:0005515;GO:0005576;GO:0007275;GO:0030154;GO:0005615;GO:0050918;GO:0001525;GO:0051781;GO:0007165;GO:0002576;GO:0031093;GO:0048010;GO:0042056;GO:0043185;GO:0001666;GO:0001938;GO:0008284;GO:0045766;GO:0050930;GO:0060754;GO:0001954;GO:0002052;GO:0006929;GO:0008285;GO:0009887;GO:0016331;GO:0030947;GO:0031954;GO:0042493;GO:0043536;GO:0045776;GO:0050679;GO:0050714;GO:0050731;GO:1901492;GO:1990830;
11274 GO:0016020;GO:0016021;GO:0046872;GO:0007155;GO:0005515;GO:0009986;GO:0007229;GO:0008305;GO:0046718;GO:0005925;GO:0005886;GO:0016032;GO:0007596;GO:0030198;GO:0044877;GO:0009887;GO:0007160;GO:0001618;GO:0005518;GO:0031589;GO:0001540;GO:0098639;GO:0033627;GO:0048333;GO:0034666;GO:0005178;GO:0038064;GO:0043236;GO:0043395;GO:0046982;GO:0001666;GO:0002687;GO:0006929;GO:0006971;GO:0007565;GO:0008283;GO:0010634;GO:0010694;GO:0014070;GO:0014075;GO:0014850;GO:0014911;GO:0030879;GO:0031346;GO:0032967;GO:0033343;GO:0033591;GO:0038065;GO:0042060;GO:0042493;GO:0043388;GO:0043589;GO:0045184;GO:0045727;GO:0045785;GO:0045987;GO:0048041;GO:0048661;GO:0050729;GO:0050927;GO:0050966;GO:0051971;GO:0060100;GO:0070365;GO:0071107;GO:0071260;GO:0071392;GO:0071407;GO:0005634;GO:0009897;GO:0030424;GO:0042995;GO:0043679;GO:0045178;GO:0048471;
7450 GO:0045944;GO:0005634;GO:0006355;GO:0006366;GO:0006351;GO:0003677;GO:0005694;GO:0000785;GO:0005515;GO:0030374;GO:0045893;GO:0005925;GO:0003700;GO:0000981;GO:0005654;GO:0005829;GO:0008285;GO:0042974;GO:0009615;GO:0006284;GO:0019899;GO:0008134;GO:0045892;GO:0003682;GO:0090575;GO:0065003;GO:0005667;GO:0051169;GO:0075713;GO:0001205;GO:0006337;GO:0046965;GO:0001158;GO:0003680;GO:0003906;GO:0042975;GO:0006268;GO:0031936;GO:0035986;GO:0090402;GO:2000774;GO:0035985;
2910 GO:0005737;GO:0016787;GO:0005576;GO:0008233;GO:0006508;GO:0004252;GO:0005615;GO:0008236;GO:0005515;GO:0070062;GO:0005102;GO:0031639;GO:0009986;GO:0007596;GO:0006464;GO:0045861;GO:0042730;GO:0051219;GO:0001666;GO:0014909;GO:0048008;GO:0030141;GO:0045177;
457 GO:0070374;GO:0016020;GO:0016021;GO:0005794;GO:0005829;GO:0007155;GO:0005886;GO:0042995;GO:0005515;GO:0005540;GO:0051216;GO:0009986;GO:0005925;GO:0050900;GO:0005887;GO:0070062;GO:0005902;GO:0043312;GO:0016324;GO:0033138;GO:0043066;GO:0050731;GO:0030667;GO:0030198;GO:0043154;GO:0060333;GO:0044319;GO:0022617;GO:0007160;GO:0044344;GO:0098609;GO:0005518;GO:0043518;GO:1902166;GO:0030214;GO:0034116;GO:0070487;GO:0004896;GO:0035692;GO:0031258;GO:2000392;GO:1900625;;GO:0033674
46915
7759 GO:0016020;GO:0016021;GO:0016740;GO:0005794;GO:0008376;GO:0032580;GO:0033842;
3928 GO:0000287;GO:0006096;GO:0004634;GO:0000015;GO:0005737;GO:0016020;GO:0046872;GO:0005829;GO:0016829;GO:0005886;GO:0005515;GO:0005615;GO:0070062;GO:0006094;GO:0061621;;GO:0001917;GO:0005622;GO:0043025;GO:0043204;GO:0043209
11706 GO:0005634;GO:0016607;GO:0006355;GO:0006351;GO:0003677;GO:0003700;GO:0005515;GO:0006974;GO:0007049;GO:0005667;GO:0042802;GO:0000122;GO:0000981;GO:0000987;GO:0003714;GO:0001890;GO:0002040;GO:0006977;GO:0032466;GO:0032877;GO:0045944;GO:0060707;GO:0060718;GO:0070365;GO:0005654;GO:0008285;GO:0030330;GO:2000134;GO:0000977;GO:0000978;GO:0001227;GO:0071930;
5642 GO:0045944;GO:0005634;GO:0005654;GO:0006355;GO:0006351;GO:0003677;GO:0003700;GO:0006334;GO:0000981;GO:0005515;GO:0007165;GO:0051216;GO:0045893;GO:0007283;GO:0001934;GO:0001501;GO:0090090;GO:0003682;GO:0008584;GO:0032991;GO:0071347;GO:0071260;GO:0008284;GO:0042127;GO:0042981;GO:0043066;GO:0070371;GO:0001502;GO:0032332;GO:0045892;GO:0006367;GO:0004672;GO:0019933;GO:0071560;GO:0007173;GO:0045662;GO:0060041;GO:0000978;GO:0002062;GO:1902894;GO:0071300;GO:0065003;GO:0050680;GO:0006338;GO:0001158;GO:0001077;GO:0030850;GO:0010634;GO:0050679;GO:0014068;GO:0034236;GO:0001708;GO:0002053;GO:0090103;GO:0060008;GO:0001894;GO:0071364;GO:0030279;GO:0070168;GO:0071504;GO:0003705;GO:0001837;GO:0090190;GO:0001942;GO:0003203;GO:0090184;GO:2000020;GO:0030916;GO:0061036;GO:2000741;GO:0032331;GO:0035019;GO:0000976;GO:0001228;GO:0008013;GO:0035326;GO:0043425;GO:0043565;GO:0044212;GO:0046982;GO:0097157;GO:0000122;GO:0001503;GO:0001658;GO:0002063;GO:0002683;GO:0003170;GO:0003179;GO:0003188;GO:0003413;GO:0003415;GO:0006357;GO:0007010;GO:0007219;GO:0007417;GO:0007507;GO:0008285;GO:0010564;GO:0010628;GO:0010629;GO:0014032;GO:0014036;GO:0019100;GO:0030154;GO:0030155;GO:0030198;GO:0030238;GO:0030502;GO:0030857;GO:0030858;GO:0030879;GO:0030903;GO:0031018;GO:0032808;GO:0034504;GO:0035622;GO:0043491;GO:0045165;GO:0045595;GO:0045732;GO:0046533;GO:0048709;GO:0048873;GO:0060009;GO:0060018;GO:0060174;GO:0060221;GO:0060350;GO:0060441;GO:0060487;GO:0060512;GO:0060517;GO:0060532;GO:0060534;GO:0060729;GO:0060784;GO:0061046;GO:0061138;GO:0061145;GO:0070384;GO:0071599;GO:0071773;GO:0072034;GO:0072170;GO:0072189;GO:0072190;GO:0072193;GO:0072197;GO:0072289;GO:0097065;GO:0098609;GO:1901203;GO:2000138;GO:2000794;GO:2001054;GO:0005667;GO:0044798;
14020 ;GO:0005737;GO:0005634;GO:0016020;GO:0000166;GO:0005524;GO:0005829;GO:0016740;GO:0016301;GO:0016310;GO:0005886;GO:0008152;GO:0003951;GO:0005515;GO:0000287;GO:0046834;GO:0003376;GO:0005516;GO:0010800;GO:0030335;GO:0031398;GO:0006457;GO:0043066;GO:0007165;GO:0051092;GO:1901224;GO:0003677;GO:0030307;GO:0035556;GO:0030148;GO:0045766;GO:0019722;GO:0045931;GO:0038036;GO:0045987;GO:0048146;GO:0046512;GO:0051721;GO:0010803;GO:0006670;GO:0008481;GO:0017050;GO:0046521;GO:0001568;GO:0006954;GO:0007420;GO:0008284;GO:0032651
2562 GO:0006629;GO:0005576;GO:0004465;GO:0052689;;GO:0005615;GO:0042632;GO:0016787;GO:0016042;GO:0004620;GO:0007584;GO:0008283;GO:0050746;GO:0004806;GO:0008201;GO:0005794;GO:0005769;GO:0034375;GO:0043691;GO:0055091;GO:0009395;GO:0009986;GO:0032376;GO:0008970;GO:0010983
2157 GO:0005634;GO:0005737;GO:0005829;GO:0006351;GO:0006355;GO:0045893;GO:0008289;GO:0070062;GO:0005543;GO:0008431;GO:0045540;
2248 GO:0046872;GO:0055114;GO:0004392;GO:0006788;GO:0005615;GO:0045766;GO:1903589;GO:0090050;GO:0005634;GO:0016020;GO:0016021;GO:0016491;GO:0005783;GO:0005789;GO:0042803;GO:0005515;GO:0006915;GO:0048471;GO:0043231;GO:0051260;GO:0043123;GO:0019221;GO:0019899;GO:0001525;GO:0048661;GO:0071456;GO:1902042;GO:0020037;GO:0034605;GO:0045765;GO:0008219;GO:0035556;GO:0048662;GO:0006979;GO:0006879;GO:0042542;GO:0034383;GO:0045080;GO:0055072;GO:0004630;GO:0001666;GO:0001935;GO:0002246;GO:0002686;GO:0007264;GO:0007588;GO:0008217;GO:0008285;GO:0008630;GO:0010656;GO:0014806;GO:0016239;GO:0016242;GO:0031670;GO:0032764;GO:0034101;GO:0034395;GO:0035094;GO:0042167;GO:0042168;GO:0043065;GO:0043305;GO:0043392;GO:0043433;GO:0043524;GO:0043619;GO:0043627;GO:0051090;GO:0071243;GO:0071276;GO:0072719;GO:0097421;GO:0097755;GO:1904036;GO:1904706;GO:0005730;GO:0005829;GO:0005901;
go_descrip
31021
4381 membrane;integral component of membrane;centrosome;nucleoplasm;cell adhesion;cell junction;protein binding;integrin-mediated signaling pathway;cell-matrix adhesion;integrin complex;viral entry into host cell;focal adhesion;plasma membrane;signaling receptor activity;integrin binding;viral process;extracellular matrix organization;virus receptor activity;receptor complex;cell adhesion mediated by integrin;inflammatory response;transforming growth factor-beta secretion;external side of plasma membrane;integrin alphav-beta6 complex;
7662 ;cytoplasm;transferase activity;protein ubiquitination;ubiquitin-protein transferase activity;cytoskeleton;spindle;ubiquitin protein ligase activity;proteasome-mediated ubiquitin-dependent protein catabolic process;protein binding;mitotic spindle;regulation of mitotic metaphase/anaphase transition
28538
8570 nucleus;protein dimerization activity;regulation of transcription, DNA-templated;transcription, DNA-templated;DNA binding;multicellular organism development;cell differentiation;RNA polymerase II transcription factor activity, sequence-specific DNA binding;transcription factor binding;DNA binding transcription factor activity;transcription cofactor activity;transcription factor complex;RNA polymerase II regulatory region sequence-specific DNA binding;transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding;negative regulation of transcription by RNA polymerase II;regulation of transcription by RNA polymerase II;nervous system development;
13727 membrane;integral component of membrane;transporter activity;transmembrane transport;plasma membrane;sodium-independent organic anion transmembrane transporter activity;sodium-independent organic anion transport;integral component of plasma membrane;lipid transporter activity;lipid transport;prostaglandin transmembrane transporter activity;prostaglandin transport;ion transport;
4845 ;Golgi apparatus;cell adhesion;ossification;extracellular space;embryo implantation;regulation of signaling receptor activity;protein binding;extracellular region;cytokine activity;extracellular exosome;biomineral tissue development;cellular protein metabolic process;post-translational protein modification;endoplasmic reticulum lumen;extracellular matrix organization;positive regulation of transcription, DNA-templated;response to vitamin D;decidualization;extracellular matrix binding;osteoblast differentiation;androgen catabolic process;positive regulation of bone resorption;cellular response to testosterone stimulus;positive regulation of estradiol secretion;inflammatory response;response to organic substance;cell differentiation;response to steroid hormone;negative regulation of collateral sprouting of intact axon in response to injury;vesicle;cell projection;perinuclear region of cytoplasm
9313 membrane;growth factor activity;regulation of signaling receptor activity;protein binding;extracellular region;multicellular organism development;cell differentiation;extracellular space;positive chemotaxis;angiogenesis;positive regulation of cell division;signal transduction;platelet degranulation;platelet alpha granule lumen;vascular endothelial growth factor receptor signaling pathway;chemoattractant activity;vascular endothelial growth factor receptor 3 binding;response to hypoxia;positive regulation of endothelial cell proliferation;positive regulation of cell proliferation;positive regulation of angiogenesis;induction of positive chemotaxis;positive regulation of mast cell chemotaxis;positive regulation of cell-matrix adhesion;positive regulation of neuroblast proliferation;substrate-dependent cell migration;negative regulation of cell proliferation;animal organ morphogenesis;morphogenesis of embryonic epithelium;regulation of vascular endothelial growth factor receptor signaling pathway;positive regulation of protein autophosphorylation;response to drug;positive regulation of blood vessel endothelial cell migration;negative regulation of blood pressure;positive regulation of epithelial cell proliferation;positive regulation of protein secretion;positive regulation of peptidyl-tyrosine phosphorylation;positive regulation of lymphangiogenesis;cellular response to leukemia inhibitory factor;
11274 membrane;integral component of membrane;metal ion binding;cell adhesion;protein binding;cell surface;integrin-mediated signaling pathway;integrin complex;viral entry into host cell;focal adhesion;plasma membrane;viral process;blood coagulation;extracellular matrix organization;protein-containing complex binding;animal organ morphogenesis;cell-matrix adhesion;virus receptor activity;collagen binding;cell-substrate adhesion;amyloid-beta binding;collagen binding involved in cell-matrix adhesion;cell adhesion mediated by integrin;mesodermal cell differentiation;integrin alpha2-beta1 complex;integrin binding;collagen receptor activity;laminin binding;heparan sulfate proteoglycan binding;protein heterodimerization activity;response to hypoxia;positive regulation of leukocyte migration;substrate-dependent cell migration;hypotonic response;female pregnancy;cell proliferation;positive regulation of epithelial cell migration;positive regulation of alkaline phosphatase activity;response to organic cyclic compound;response to amine;response to muscle activity;positive regulation of smooth muscle cell migration;mammary gland development;positive regulation of cell projection organization;positive regulation of collagen biosynthetic process;positive regulation of collagen binding;response to L-ascorbic acid;collagen-activated signaling pathway;wound healing;response to drug;positive regulation of DNA binding;skin morphogenesis;establishment of protein localization;positive regulation of translation;positive regulation of cell adhesion;positive regulation of smooth muscle contraction;focal adhesion assembly;positive regulation of smooth muscle cell proliferation;positive regulation of inflammatory response;positive regulation of positive chemotaxis;detection of mechanical stimulus involved in sensory perception of pain;positive regulation of transmission of nerve impulse;positive regulation of phagocytosis, engulfment;hepatocyte differentiation;response to parathyroid hormone;cellular response to mechanical stimulus;cellular response to estradiol stimulus;cellular response to organic cyclic compound;nucleus;external side of plasma membrane;axon;cell projection;axon terminus;basal part of cell;perinuclear region of cytoplasm;
7450 positive regulation of transcription by RNA polymerase II;nucleus;regulation of transcription, DNA-templated;transcription by RNA polymerase II;transcription, DNA-templated;DNA binding;chromosome;chromatin;protein binding;ligand-dependent nuclear receptor transcription coactivator activity;positive regulation of transcription, DNA-templated;focal adhesion;DNA binding transcription factor activity;RNA polymerase II transcription factor activity, sequence-specific DNA binding;nucleoplasm;cytosol;negative regulation of cell proliferation;retinoic acid receptor binding;response to virus;base-excision repair;enzyme binding;transcription factor binding;negative regulation of transcription, DNA-templated;chromatin binding;RNA polymerase II transcription factor complex;protein-containing complex assembly;transcription factor complex;nuclear transport;establishment of integrated proviral latency;transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific DNA binding;nucleosome disassembly;retinoid X receptor binding;enhancer sequence-specific DNA binding;AT DNA binding;DNA-(apurinic or apyrimidinic site) endonuclease activity;peroxisome proliferator activated receptor binding;DNA unwinding involved in DNA replication;negative regulation of chromatin silencing;senescence-associated heterochromatin focus assembly;oncogene-induced cell senescence;positive regulation of cellular senescence;senescence-associated heterochromatin focus;
2910 cytoplasm;hydrolase activity;extracellular region;peptidase activity;proteolysis;serine-type endopeptidase activity;extracellular space;serine-type peptidase activity;protein binding;extracellular exosome;signaling receptor binding;plasminogen activation;cell surface;blood coagulation;cellular protein modification process;negative regulation of proteolysis;fibrinolysis;phosphoprotein binding;response to hypoxia;smooth muscle cell migration;platelet-derived growth factor receptor signaling pathway;secretory granule;apical part of cell;
457 positive regulation of ERK1 and ERK2 cascade;membrane;integral component of membrane;Golgi apparatus;cytosol;cell adhesion;plasma membrane;cell projection;protein binding;hyaluronic acid binding;cartilage development;cell surface;focal adhesion;leukocyte migration;integral component of plasma membrane;extracellular exosome;microvillus;neutrophil degranulation;apical plasma membrane;positive regulation of peptidyl-serine phosphorylation;negative regulation of apoptotic process;positive regulation of peptidyl-tyrosine phosphorylation;secretory granule membrane;extracellular matrix organization;negative regulation of cysteine-type endopeptidase activity involved in apoptotic process;interferon-gamma-mediated signaling pathway;wound healing, spreading of cells;extracellular matrix disassembly;cell-matrix adhesion;cellular response to fibroblast growth factor stimulus;cell-cell adhesion;collagen binding;negative regulation of DNA damage response, signal transduction by p53 class mediator;negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator;hyaluronan catabolic process;positive regulation of heterotypic cell-cell adhesion;monocyte aggregation;cytokine receptor activity;macrophage migration inhibitory factor receptor complex;lamellipodium membrane;regulation of lamellipodium morphogenesis;positive regulation of monocyte aggregation;;positive regulation of kinase activity
46915
7759 membrane;integral component of membrane;transferase activity;Golgi apparatus;acetylgalactosaminyltransferase activity;Golgi cisterna membrane;N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase activity;
3928 magnesium ion binding;glycolytic process;phosphopyruvate hydratase activity;phosphopyruvate hydratase complex;cytoplasm;membrane;metal ion binding;cytosol;lyase activity;plasma membrane;protein binding;extracellular space;extracellular exosome;gluconeogenesis;canonical glycolysis;;photoreceptor inner segment;intracellular;neuronal cell body;perikaryon;myelin sheath
11706 nucleus;nuclear speck;regulation of transcription, DNA-templated;transcription, DNA-templated;DNA binding;DNA binding transcription factor activity;protein binding;cellular response to DNA damage stimulus;cell cycle;transcription factor complex;identical protein binding;negative regulation of transcription by RNA polymerase II;RNA polymerase II transcription factor activity, sequence-specific DNA binding;proximal promoter sequence-specific DNA binding;transcription corepressor activity;placenta development;sprouting angiogenesis;DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest;negative regulation of cytokinesis;positive regulation of DNA endoreduplication;positive regulation of transcription by RNA polymerase II;trophoblast giant cell differentiation;chorionic trophoblast cell differentiation;hepatocyte differentiation;nucleoplasm;negative regulation of cell proliferation;DNA damage response, signal transduction by p53 class mediator;negative regulation of G1/S transition of mitotic cell cycle;RNA polymerase II regulatory region sequence-specific DNA binding;RNA polymerase II proximal promoter sequence-specific DNA binding;transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding;negative regulation of transcription involved in G1/S transition of mitotic cell cycle;
5642 positive regulation of transcription by RNA polymerase II;nucleus;nucleoplasm;regulation of transcription, DNA-templated;transcription, DNA-templated;DNA binding;DNA binding transcription factor activity;nucleosome assembly;RNA polymerase II transcription factor activity, sequence-specific DNA binding;protein binding;signal transduction;cartilage development;positive regulation of transcription, DNA-templated;spermatogenesis;positive regulation of protein phosphorylation;skeletal system development;negative regulation of canonical Wnt signaling pathway;chromatin binding;male gonad development;protein-containing complex;cellular response to interleukin-1;cellular response to mechanical stimulus;positive regulation of cell proliferation;regulation of cell proliferation;regulation of apoptotic process;negative regulation of apoptotic process;ERK1 and ERK2 cascade;cartilage condensation;positive regulation of chondrocyte differentiation;negative regulation of transcription, DNA-templated;transcription initiation from RNA polymerase II promoter;protein kinase activity;cAMP-mediated signaling;cellular response to transforming growth factor beta stimulus;epidermal growth factor receptor signaling pathway;negative regulation of myoblast differentiation;retina development in camera-type eye;RNA polymerase II proximal promoter sequence-specific DNA binding;chondrocyte differentiation;negative regulation of pri-miRNA transcription by RNA polymerase II;cellular response to retinoic acid;protein-containing complex assembly;negative regulation of epithelial cell proliferation;chromatin remodeling;enhancer sequence-specific DNA binding;transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding;prostate gland development;positive regulation of epithelial cell migration;positive regulation of epithelial cell proliferation;positive regulation of phosphatidylinositol 3-kinase signaling;protein kinase A catalytic subunit binding;cell fate specification;positive regulation of mesenchymal cell proliferation;cochlea morphogenesis;Sertoli cell differentiation;tissue homeostasis;cellular response to epidermal growth factor stimulus;negative regulation of ossification;negative regulation of biomineral tissue development;cellular response to heparin;transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding;epithelial to mesenchymal transition;positive regulation of branching involved in ureteric bud morphogenesis;hair follicle development;endocardial cushion morphogenesis;positive regulation of kidney development;positive regulation of male gonad development;otic vesicle formation;positive regulation of cartilage development;positive regulation of mesenchymal stem cell differentiation;negative regulation of chondrocyte differentiation;somatic stem cell population maintenance;transcription regulatory region sequence-specific DNA binding;transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding;beta-catenin binding;enhancer binding;bHLH transcription factor binding;sequence-specific DNA binding;transcription regulatory region DNA binding;protein heterodimerization activity;pre-mRNA intronic binding;negative regulation of transcription by RNA polymerase II;ossification;branching involved in ureteric bud morphogenesis;chondrocyte development;negative regulation of immune system process;heart valve development;heart valve morphogenesis;heart valve formation;chondrocyte differentiation involved in endochondral bone morphogenesis;chondrocyte hypertrophy;regulation of transcription by RNA polymerase II;cytoskeleton organization;Notch signaling pathway;central nervous system development;heart development;negative regulation of cell proliferation;regulation of cell cycle process;positive regulation of gene expression;negative regulation of gene expression;neural crest cell development;neural crest cell fate specification;male germ-line sex determination;cell differentiation;regulation of cell adhesion;extracellular matrix organization;male sex determination;negative regulation of bone mineralization;negative regulation of epithelial cell differentiation;positive regulation of epithelial cell differentiation;mammary gland development;notochord development;endocrine pancreas development;lacrimal gland development;protein localization to nucleus;intrahepatic bile duct development;protein kinase B signaling;cell fate commitment;regulation of cell differentiation;positive regulation of protein catabolic process;negative regulation of photoreceptor cell differentiation;oligodendrocyte differentiation;homeostasis of number of cells within a tissue;Sertoli cell development;astrocyte fate commitment;limb bud formation;retinal rod cell differentiation;endochondral bone morphogenesis;epithelial tube branching involved in lung morphogenesis;lung epithelial cell differentiation;prostate gland morphogenesis;epithelial cell proliferation involved in prostatic bud elongation;bronchus cartilage development;trachea cartilage development;intestinal epithelial structure maintenance;regulation of cell proliferation involved in tissue homeostasis;regulation of branching involved in lung morphogenesis;morphogenesis of a branching epithelium;lung smooth muscle development;Harderian gland development;otic vesicle development;cellular response to BMP stimulus;renal vesicle induction;metanephric tubule development;ureter development;ureter urothelium development;ureter smooth muscle cell differentiation;ureter morphogenesis;metanephric nephron tubule formation;anterior head development;cell-cell adhesion;positive regulation of extracellular matrix assembly;positive regulation of cell proliferation involved in heart morphogenesis;regulation of epithelial cell proliferation involved in lung morphogenesis;negative regulation of mesenchymal cell apoptotic process;transcription factor complex;nuclear transcription factor complex;
14020 ;cytoplasm;nucleus;membrane;nucleotide binding;ATP binding;cytosol;transferase activity;kinase activity;phosphorylation;plasma membrane;metabolic process;NAD+ kinase activity;protein binding;magnesium ion binding;lipid phosphorylation;sphingosine-1-phosphate signaling pathway;calmodulin binding;positive regulation of peptidyl-threonine phosphorylation;positive regulation of cell migration;positive regulation of protein ubiquitination;protein folding;negative regulation of apoptotic process;signal transduction;positive regulation of NF-kappaB transcription factor activity;positive regulation of NIK/NF-kappaB signaling;DNA binding;positive regulation of cell growth;intracellular signal transduction;sphingolipid biosynthetic process;positive regulation of angiogenesis;calcium-mediated signaling;positive regulation of mitotic cell cycle;sphingosine-1-phosphate receptor activity;positive regulation of smooth muscle contraction;positive regulation of fibroblast proliferation;sphingosine biosynthetic process;protein phosphatase 2A binding;regulation of tumor necrosis factor-mediated signaling pathway;sphingosine metabolic process;sphinganine kinase activity;D-erythro-sphingosine kinase activity;sphingoid catabolic process;blood vessel development;inflammatory response;brain development;positive regulation of cell proliferation;regulation of interleukin-1 beta production
2562 lipid metabolic process;extracellular region;lipoprotein lipase activity;carboxylic ester hydrolase activity;;extracellular space;cholesterol homeostasis;hydrolase activity;lipid catabolic process;phospholipase activity;response to nutrient;cell proliferation;regulation of lipoprotein metabolic process;triglyceride lipase activity;heparin binding;Golgi apparatus;early endosome;high-density lipoprotein particle remodeling;reverse cholesterol transport;phospholipid homeostasis;phospholipid catabolic process;cell surface;positive regulation of cholesterol transport;phosphatidylcholine 1-acylhydrolase activity;positive regulation of high-density lipoprotein particle clearance
2157 nucleus;cytoplasm;cytosol;transcription, DNA-templated;regulation of transcription, DNA-templated;positive regulation of transcription, DNA-templated;lipid binding;extracellular exosome;phospholipid binding;vitamin E binding;regulation of cholesterol biosynthetic process;
2248 metal ion binding;oxidation-reduction process;heme oxygenase (decyclizing) activity;heme oxidation;extracellular space;positive regulation of angiogenesis;positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis;positive regulation of cell migration involved in sprouting angiogenesis;nucleus;membrane;integral component of membrane;oxidoreductase activity;endoplasmic reticulum;endoplasmic reticulum membrane;protein homodimerization activity;protein binding;apoptotic process;perinuclear region of cytoplasm;intracellular membrane-bounded organelle;protein homooligomerization;positive regulation of I-kappaB kinase/NF-kappaB signaling;cytokine-mediated signaling pathway;enzyme binding;angiogenesis;positive regulation of smooth muscle cell proliferation;cellular response to hypoxia;negative regulation of extrinsic apoptotic signaling pathway via death domain receptors;heme binding;cellular response to heat;regulation of angiogenesis;cell death;intracellular signal transduction;negative regulation of smooth muscle cell proliferation;response to oxidative stress;cellular iron ion homeostasis;response to hydrogen peroxide;low-density lipoprotein particle clearance;positive regulation of chemokine biosynthetic process;iron ion homeostasis;phospholipase D activity;response to hypoxia;endothelial cell proliferation;wound healing involved in inflammatory response;negative regulation of leukocyte migration;small GTPase mediated signal transduction;excretion;regulation of blood pressure;negative regulation of cell proliferation;intrinsic apoptotic signaling pathway in response to DNA damage;negative regulation of muscle cell apoptotic process;smooth muscle hyperplasia;positive regulation of macroautophagy;negative regulation of macroautophagy;cellular response to nutrient;negative regulation of mast cell cytokine production;erythrocyte homeostasis;regulation of transcription from RNA polymerase II promoter in response to iron;response to nicotine;heme catabolic process;heme metabolic process;positive regulation of apoptotic process;negative regulation of mast cell degranulation;negative regulation of DNA binding;negative regulation of DNA binding transcription factor activity;negative regulation of neuron apoptotic process;regulation of transcription from RNA polymerase II promoter in response to oxidative stress;response to estrogen;regulation of DNA binding transcription factor activity;cellular response to arsenic-containing substance;cellular response to cadmium ion;cellular response to cisplatin;liver regeneration;positive regulation of blood vessel diameter;negative regulation of epithelial cell apoptotic process;negative regulation of vascular smooth muscle cell proliferation;nucleolus;cytosol;caveola;
plot_gene <- function(v, g) {
# v - An EList object containing log2 counts per million
# g - character vector of a single gene
stopifnot(class(v) == "EList",
is.character(g), length(g) == 1)
library("tidyr")
single_gene <- v$E[g, ]
single_gene_long <- as.data.frame(single_gene)
colnames(single_gene_long) <- "log2cpm"
single_gene_long$sample <- rownames(single_gene_long)
single_gene_long <- separate(single_gene_long, col = "sample", sep = "_",
into = c("Individual", "Replicate", "Treatment"))
single_gene_long$Treatment <- gsub('S', 'Strain', single_gene_long$Treatment)
single_gene_long$Treatment <- gsub('U', 'Control', single_gene_long$Treatment)
single_gene_long$Treatment <- factor(single_gene_long$Treatment, levels(factor(single_gene_long$Treatment))[c(1,2)])
return(ggplot(single_gene_long, aes(x = Individual, y = log2cpm, fill = Individual)) +
labs(title = g, y = expression("Expression level (" * log[2] * " cpm)")) +
geom_dotplot(binaxis = "y", stackdir = "center", key_glyph = 'rect', dotsize = 0.75) +
facet_wrap(~Treatment, strip.position = 'bottom') +
stat_summary(fun.data = "mean_sdl", fun.args = list(mult=1)) +
theme(axis.title.x=element_blank())
)
}
#all top genes
for (gene in 1:length(significant_anno_05_FC1[,1])) {
print(plot_gene(v2, as.character(significant_anno_05_FC1[gene,1])))
}
`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

#SOX9
plot_gene(v2, "ENSG00000125398")
`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

#all top upregulated
for (gene in 1:length(significant_anno_05_FC1_up[,1])) {
print(plot_gene(v2, as.character(significant_anno_05_FC1_up[gene,1])))
}
`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

#all top downregulated
for (gene in 1:length(significant_anno_05_FC1_down[,1])) {
print(plot_gene(v2, as.character(significant_anno_05_FC1_down[gene,1])))
}
`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

`stat_bindot()` using `bins = 30`. Pick better value with `binwidth`.

Use topGO for GO analysis. It accounts for the nested graph structure of GO terms to prune the number of GO categories tested (Alexa et al. 2006). Essentially, it decreases the redundancy of the results.
First create the gene universe. This is all the genes tested for differential expression assigned a 1 for differentially expressed and 0 if not.
saveRDS(results, "data/DE_results.rds")
threshold <- 0.05
gene_universe <- as.numeric(results[["Unstrain"]]$qvalue < threshold)
gene_universe <- factor(gene_universe)
names(gene_universe) <- rownames(results[["Unstrain"]])
head(gene_universe)
ENSG00000225972 ENSG00000225630 ENSG00000237973 ENSG00000248527
0 0 0 0
ENSG00000198744 ENSG00000228794
0 0
Levels: 0 1
Create the topGO data object. Only consider “Biological Process” categories and use the Mouse Ensembl database for annotation.
go_data <- new("topGOdata",
ontology = "BP",
allGenes = gene_universe,
nodeSize = 5,
annotationFun = annFUN.org,
mapping = "org.Hs.eg.db",
ID = "ensembl")
Building most specific GOs .....
Loading required package: org.Hs.eg.db
( 9790 GO terms found. )
Build GO DAG topology ..........
( 13875 GO terms and 32681 relations. )
Annotating nodes ...............
( 9223 genes annotated to the GO terms. )
Use the weight01 algorithm and score the tests with Fisher’s exact test.
go_test <- runTest(go_data, algorithm = "weight01", statistic = "fisher")
-- Weight01 Algorithm --
the algorithm is scoring 6032 nontrivial nodes
parameters:
test statistic: fisher
Level 19: 1 nodes to be scored (0 eliminated genes)
Level 18: 3 nodes to be scored (0 eliminated genes)
Level 17: 7 nodes to be scored (11 eliminated genes)
Level 16: 20 nodes to be scored (17 eliminated genes)
Level 15: 51 nodes to be scored (51 eliminated genes)
Level 14: 108 nodes to be scored (199 eliminated genes)
Level 13: 175 nodes to be scored (574 eliminated genes)
Level 12: 295 nodes to be scored (1404 eliminated genes)
Level 11: 496 nodes to be scored (2930 eliminated genes)
Level 10: 677 nodes to be scored (4305 eliminated genes)
Level 9: 807 nodes to be scored (5435 eliminated genes)
Level 8: 845 nodes to be scored (6766 eliminated genes)
Level 7: 903 nodes to be scored (7583 eliminated genes)
Level 6: 782 nodes to be scored (8202 eliminated genes)
Level 5: 476 nodes to be scored (8583 eliminated genes)
Level 4: 252 nodes to be scored (8827 eliminated genes)
Level 3: 111 nodes to be scored (8980 eliminated genes)
Level 2: 22 nodes to be scored (9028 eliminated genes)
Level 1: 1 nodes to be scored (9091 eliminated genes)
Keep the results with a Fisher’s exact test p-value < 0.01.
go_table <- GenTable(go_data, weightFisher = go_test,
orderBy = "weightFisher", ranksOf = "weightFisher",
topNodes = sum(score(go_test) < .01))
go_table
GO.ID Term Annotated
1 GO:0055114 oxidation-reduction process 605
2 GO:0030198 extracellular matrix organization 208
3 GO:0045454 cell redox homeostasis 47
4 GO:0001568 blood vessel development 434
5 GO:0046364 monosaccharide biosynthetic process 63
6 GO:0050927 positive regulation of positive chemotax... 10
7 GO:0034135 regulation of toll-like receptor 2 signa... 7
8 GO:0006027 glycosaminoglycan catabolic process 40
9 GO:0006986 response to unfolded protein 141
10 GO:0051085 chaperone cofactor-dependent protein ref... 11
11 GO:1903364 positive regulation of cellular protein ... 108
12 GO:0048842 positive regulation of axon extension in... 5
13 GO:0002576 platelet degranulation 72
14 GO:0002063 chondrocyte development 21
15 GO:0033627 cell adhesion mediated by integrin 38
16 GO:0021955 central nervous system neuron axonogenes... 25
17 GO:0035987 endodermal cell differentiation 31
18 GO:0006695 cholesterol biosynthetic process 56
19 GO:0051897 positive regulation of protein kinase B ... 77
20 GO:0030223 neutrophil differentiation 6
21 GO:0006516 glycoprotein catabolic process 14
22 GO:0045214 sarcomere organization 19
23 GO:0060390 regulation of SMAD protein signal transd... 18
24 GO:0019321 pentose metabolic process 7
25 GO:0043589 skin morphogenesis 7
26 GO:0036499 PERK-mediated unfolded protein response 16
27 GO:0051693 actin filament capping 18
28 GO:0001895 retina homeostasis 27
29 GO:0030335 positive regulation of cell migration 300
30 GO:0051702 interaction with symbiont 53
31 GO:0060047 heart contraction 113
32 GO:0045056 transcytosis 11
33 GO:0048708 astrocyte differentiation 36
34 GO:0031346 positive regulation of cell projection o... 245
35 GO:0044267 cellular protein metabolic process 3279
36 GO:0007275 multicellular organism development 2843
37 GO:0034329 cell junction assembly 151
38 GO:0006098 pentose-phosphate shunt 12
39 GO:1901700 response to oxygen-containing compound 904
40 GO:0060706 cell differentiation involved in embryon... 15
41 GO:0032964 collagen biosynthetic process 29
42 GO:0033692 cellular polysaccharide biosynthetic pro... 39
43 GO:0008360 regulation of cell shape 81
Significant Expected weightFisher
1 101 68.68 3.0e-07
2 50 23.61 1.6e-05
3 16 5.34 3.3e-05
4 72 49.27 0.00014
5 13 7.15 0.00016
6 6 1.14 0.00030
7 5 0.79 0.00032
8 11 4.54 0.00039
9 32 16.01 0.00048
10 6 1.25 0.00059
11 21 12.26 0.00063
12 4 0.57 0.00075
13 18 8.17 0.00089
14 8 2.38 0.00141
15 10 4.31 0.00158
16 6 2.84 0.00159
17 10 3.52 0.00161
18 16 6.36 0.00161
19 18 8.74 0.00203
20 4 0.68 0.00205
21 6 1.59 0.00283
22 7 2.16 0.00350
23 7 2.04 0.00433
24 4 0.79 0.00436
25 4 0.79 0.00436
26 5 1.82 0.00436
27 5 2.04 0.00436
28 6 3.07 0.00481
29 51 34.06 0.00528
30 10 6.02 0.00532
31 16 12.83 0.00533
32 4 1.25 0.00534
33 6 4.09 0.00535
34 29 27.81 0.00536
35 365 372.23 0.00619
36 374 322.74 0.00664
37 21 17.14 0.00745
38 5 1.36 0.00746
39 115 102.62 0.00765
40 6 1.70 0.00790
41 6 3.29 0.00794
42 7 4.43 0.00795
43 17 9.20 0.00850
go_table[,-1]
Term Annotated Significant
1 oxidation-reduction process 605 101
2 extracellular matrix organization 208 50
3 cell redox homeostasis 47 16
4 blood vessel development 434 72
5 monosaccharide biosynthetic process 63 13
6 positive regulation of positive chemotax... 10 6
7 regulation of toll-like receptor 2 signa... 7 5
8 glycosaminoglycan catabolic process 40 11
9 response to unfolded protein 141 32
10 chaperone cofactor-dependent protein ref... 11 6
11 positive regulation of cellular protein ... 108 21
12 positive regulation of axon extension in... 5 4
13 platelet degranulation 72 18
14 chondrocyte development 21 8
15 cell adhesion mediated by integrin 38 10
16 central nervous system neuron axonogenes... 25 6
17 endodermal cell differentiation 31 10
18 cholesterol biosynthetic process 56 16
19 positive regulation of protein kinase B ... 77 18
20 neutrophil differentiation 6 4
21 glycoprotein catabolic process 14 6
22 sarcomere organization 19 7
23 regulation of SMAD protein signal transd... 18 7
24 pentose metabolic process 7 4
25 skin morphogenesis 7 4
26 PERK-mediated unfolded protein response 16 5
27 actin filament capping 18 5
28 retina homeostasis 27 6
29 positive regulation of cell migration 300 51
30 interaction with symbiont 53 10
31 heart contraction 113 16
32 transcytosis 11 4
33 astrocyte differentiation 36 6
34 positive regulation of cell projection o... 245 29
35 cellular protein metabolic process 3279 365
36 multicellular organism development 2843 374
37 cell junction assembly 151 21
38 pentose-phosphate shunt 12 5
39 response to oxygen-containing compound 904 115
40 cell differentiation involved in embryon... 15 6
41 collagen biosynthetic process 29 6
42 cellular polysaccharide biosynthetic pro... 39 7
43 regulation of cell shape 81 17
Expected weightFisher
1 68.68 3.0e-07
2 23.61 1.6e-05
3 5.34 3.3e-05
4 49.27 0.00014
5 7.15 0.00016
6 1.14 0.00030
7 0.79 0.00032
8 4.54 0.00039
9 16.01 0.00048
10 1.25 0.00059
11 12.26 0.00063
12 0.57 0.00075
13 8.17 0.00089
14 2.38 0.00141
15 4.31 0.00158
16 2.84 0.00159
17 3.52 0.00161
18 6.36 0.00161
19 8.74 0.00203
20 0.68 0.00205
21 1.59 0.00283
22 2.16 0.00350
23 2.04 0.00433
24 0.79 0.00436
25 0.79 0.00436
26 1.82 0.00436
27 2.04 0.00436
28 3.07 0.00481
29 34.06 0.00528
30 6.02 0.00532
31 12.83 0.00533
32 1.25 0.00534
33 4.09 0.00535
34 27.81 0.00536
35 372.23 0.00619
36 322.74 0.00664
37 17.14 0.00745
38 1.36 0.00746
39 102.62 0.00765
40 1.70 0.00790
41 3.29 0.00794
42 4.43 0.00795
43 9.20 0.00850
write.csv(go_table, "output/GO_terms_enriched.csv")
# k-s test making use of ranked based on p-values
genes <- results[["Unstrain"]]$qvalue
names(genes) <- rownames(results[["Unstrain"]])
selection <- function(allScore){ return(allScore < 0.05)} # function that returns TRUE/FALSE for p-values<0.05
allGO2genes <- annFUN.org(whichOnto="BP", feasibleGenes=NULL, mapping="org.Hs.eg.db", ID="ensembl")
GOdata <- new("topGOdata",
ontology="BP",
allGenes=genes,
annot=annFUN.GO2genes,
GO2genes=allGO2genes,
geneSel=selection,
nodeSize=10)
Building most specific GOs .....
( 9790 GO terms found. )
Build GO DAG topology ..........
( 13875 GO terms and 32681 relations. )
Annotating nodes ...............
( 9223 genes annotated to the GO terms. )
results.ks <- runTest(GOdata, algorithm="classic", statistic="ks")
-- Classic Algorithm --
the algorithm is scoring 5184 nontrivial nodes
parameters:
test statistic: ks
score order: increasing
goEnrichment <- GenTable(GOdata, KS=results.ks, orderBy="KS", topNodes=20)
goEnrichment <- goEnrichment %>%
mutate(KS = ifelse(grepl("<", KS), 1e-30, KS))
goEnrichment$KS <- as.numeric(goEnrichment$KS)
goEnrichment <- goEnrichment[goEnrichment$KS<0.05,]
goEnrichment <- goEnrichment[,c("GO.ID","Term","KS")]
goEnrichment$Term <- gsub(" [a-z]*\\.\\.\\.$", "", goEnrichment$Term)
goEnrichment$Term <- gsub("\\.\\.\\.$", "", goEnrichment$Term)
goEnrichment$Term <- paste(goEnrichment$GO.ID, goEnrichment$Term, sep=", ")
goEnrichment$Term <- factor(goEnrichment$Term, levels=rev(goEnrichment$Term))
goEnrichment$KS <- as.numeric(goEnrichment$KS)
require(ggplot2)
ggplot(goEnrichment, aes(x=Term, y=-log10(KS))) +
stat_summary(geom = "bar", fun = mean, position = "dodge") +
xlab("Biological process") +
ylab("Enrichment") +
ggtitle("Title") +
scale_y_continuous(breaks = round(seq(0, max(-log10(goEnrichment$KS)), by = 2), 1)) +
theme_bw(base_size=24) +
theme(
legend.position='none',
legend.background=element_rect(),
plot.title=element_text(angle=0, size=24, face="bold", vjust=1),
axis.text.x=element_text(angle=0, size=18, face="bold", hjust=1.10),
axis.text.y=element_text(angle=0, size=18, face="bold", vjust=0.5),
axis.title=element_text(size=24, face="bold"),
legend.key=element_blank(), #removes the border
legend.key.size=unit(1, "cm"), #Sets overall area/size of the legend
legend.text=element_text(size=18), #Text size
title=element_text(size=18)) +
guides(colour=guide_legend(override.aes=list(size=2.5))) +
coord_flip()

Use pathfindR to perform enrichment analysis of pathways enriched amongst DE genes
# library(pathfindR)
# #Get data in corrrect format: DF with "gene symbols", "change values" (optional), "associated p values"
# results_df <- results[["Unstrain"]] %>%
# dplyr::select(logFC, qvalue) %>%
# tibble::rownames_to_column()
#
# results_df$rowname <- gene_anno$external_gene_name[match(results_df$rowname, gene_anno$ensembl_gene_id)]
# results_df <- results_df %>%
# dplyr::mutate(Gene.symbol = rowname) %>%
# dplyr::select(Gene.symbol, logFC, qvalue)
# results_df$Gene.symbol <- as.character(results_df$Gene.symbol)
#
# Unstrain_processed <- input_processing(input = results_df, # the input: in this case, differential expression results
# p_val_threshold = 0.05, # p value threshold to filter significant genes
# pin_name_path = "Biogrid", # the name of the PIN to use for active subnetwork search
# convert2alias = TRUE) # boolean indicating whether or not to convert missing symbols to alias symbols in the PIN
#
# # using "BioCarta" as our gene sets for enrichment
# biocarta_list <- fetch_gene_set(gene_sets = "BioCarta",
# min_gset_size = 10,
# max_gset_size = 300)
# biocarta_gsets <- biocarta_list[[1]]
# biocarta_descriptions <- biocarta_list[[2]]
#
# # using "KEGG" as our gene sets for enrichment
# KEGG_list <- fetch_gene_set(gene_sets = "KEGG",
# min_gset_size = 10,
# max_gset_size = 300)
# KEGG_gsets <- KEGG_list[[1]]
# KEGG_descriptions <- KEGG_list[[2]]
#
# n_iter <- 15 ## number of iterations
# combined_res <- NULL ## to store the result of each iteration
#
# for (i in 1:n_iter) {
#
# ###### Active Subnetwork Search
# snws_file <- paste0("active_snws_", i) # Name of output file
# active_snws <- active_snw_search(input_for_search = Unstrain_processed,
# pin_name_path = "Biogrid",
# snws_file = snws_file,
# score_quan_thr = 0.8, # you may tweak these arguments for optimal filtering of subnetworks
# sig_gene_thr = 0.02, # you may tweak these arguments for optimal filtering of subnetworks
# search_method = "GR")
#
# ###### Enrichment Analyses
# current_res <- enrichment_analyses(snws = active_snws,
# sig_genes_vec = Unstrain_processed$GENE,
# pin_name_path = "Biogrid",
# genes_by_term = KEGG_gsets,
# term_descriptions = KEGG_descriptions,
# adj_method = "bonferroni",
# enrichment_threshold = 0.05,
# list_active_snw_genes = TRUE) # listing the non-input active snw genes in output
#
# ###### Combine results via `rbind`
# combined_res <- rbind(combined_res, current_res)
# }
#
#
#
# ###### Summarize Combined Enrichment Results
# summarized_df <- summarize_enrichment_results(combined_res,
# list_active_snw_genes = TRUE)
#
# ###### Annotate Affected Genes Involved in Each Enriched Term
# final_res <- annotate_term_genes(result_df = summarized_df,
# input_processed = Unstrain_processed,
# genes_by_term = KEGG_gsets)
#
# #visualization
# visualize_terms(result_df = final_res,
# hsa_KEGG = FALSE, # boolean to indicate whether human KEGG gene sets were used for enrichment analysis or not
# pin_name_path = "Biogrid")
#
# enrichment_chart(final_res, top_terms = 10)
Use topGO for GO analysis. Essentially, it decreases the redundancy of the results.
First create the gene universe for upregulated genes This is all the genes tested for differential expression assigned a 1 for differentially expressed and 0 if not.
threshold <- 0.05
gene_universe_up <- as.numeric(results[["Unstrain"]]$qvalue < threshold & results[["Unstrain"]]$logFC > 0)
gene_universe_up <- factor(gene_universe_up)
names(gene_universe_up) <- rownames(results[["Unstrain"]])
head(gene_universe_up)
ENSG00000225972 ENSG00000225630 ENSG00000237973 ENSG00000248527
0 0 0 0
ENSG00000198744 ENSG00000228794
0 0
Levels: 0 1
Create the topGO data object. Only consider “Biological Process” categories and use the human Ensembl database for annotation.
go_data <- new("topGOdata",
ontology = "BP",
allGenes = gene_universe_up,
nodeSize = 5,
annotationFun = annFUN.org,
mapping = "org.Hs.eg.db",
ID = "ensembl")
Building most specific GOs .....
( 9790 GO terms found. )
Build GO DAG topology ..........
( 13875 GO terms and 32681 relations. )
Annotating nodes ...............
( 9223 genes annotated to the GO terms. )
Use the weight01 algorithm and score the tests with Fisher’s exact test.
go_test <- runTest(go_data, algorithm = "weight01", statistic = "fisher")
-- Weight01 Algorithm --
the algorithm is scoring 4723 nontrivial nodes
parameters:
test statistic: fisher
Level 19: 1 nodes to be scored (0 eliminated genes)
Level 18: 2 nodes to be scored (0 eliminated genes)
Level 17: 6 nodes to be scored (11 eliminated genes)
Level 16: 14 nodes to be scored (16 eliminated genes)
Level 15: 27 nodes to be scored (51 eliminated genes)
Level 14: 58 nodes to be scored (166 eliminated genes)
Level 13: 116 nodes to be scored (451 eliminated genes)
Level 12: 207 nodes to be scored (1157 eliminated genes)
Level 11: 354 nodes to be scored (2713 eliminated genes)
Level 10: 515 nodes to be scored (4137 eliminated genes)
Level 9: 635 nodes to be scored (5269 eliminated genes)
Level 8: 651 nodes to be scored (6604 eliminated genes)
Level 7: 715 nodes to be scored (7458 eliminated genes)
Level 6: 657 nodes to be scored (8140 eliminated genes)
Level 5: 419 nodes to be scored (8544 eliminated genes)
Level 4: 222 nodes to be scored (8816 eliminated genes)
Level 3: 101 nodes to be scored (8974 eliminated genes)
Level 2: 22 nodes to be scored (9025 eliminated genes)
Level 1: 1 nodes to be scored (9091 eliminated genes)
Keep the results with a Fisher’s exact test p-value < 0.01.
go_table <- GenTable(go_data, weightFisher = go_test,
orderBy = "weightFisher", ranksOf = "weightFisher",
topNodes = sum(score(go_test) < .01))
go_table
GO.ID Term Annotated
1 GO:0030198 extracellular matrix organization 208
2 GO:0030199 collagen fibril organization 31
3 GO:0006027 glycosaminoglycan catabolic process 40
4 GO:0032964 collagen biosynthetic process 29
5 GO:0006768 biotin metabolic process 9
6 GO:0031643 positive regulation of myelination 9
7 GO:0001503 ossification 246
8 GO:0006024 glycosaminoglycan biosynthetic process 75
9 GO:0045454 cell redox homeostasis 47
10 GO:0044267 cellular protein metabolic process 3279
11 GO:0035987 endodermal cell differentiation 31
12 GO:0060972 left/right pattern formation 14
13 GO:0060638 mesenchymal-epithelial cell signaling 5
14 GO:0070208 protein heterotrimerization 11
15 GO:0042362 fat-soluble vitamin biosynthetic process 8
16 GO:0010838 positive regulation of keratinocyte prol... 6
17 GO:0098974 postsynaptic actin cytoskeleton organiza... 6
18 GO:0048709 oligodendrocyte differentiation 55
19 GO:0034329 cell junction assembly 151
20 GO:0003009 skeletal muscle contraction 13
21 GO:0060390 regulation of SMAD protein signal transd... 18
22 GO:0051764 actin crosslink formation 7
23 GO:0060346 bone trabecula formation 7
24 GO:0001649 osteoblast differentiation 150
25 GO:0033692 cellular polysaccharide biosynthetic pro... 39
26 GO:0009058 biosynthetic process 3911
27 GO:0042476 odontogenesis 77
28 GO:0048592 eye morphogenesis 84
29 GO:0085029 extracellular matrix assembly 16
30 GO:2000345 regulation of hepatocyte proliferation 8
Significant Expected weightFisher
1 39 12.02 6.1e-06
2 8 1.79 0.00028
3 9 2.31 0.00030
4 5 1.68 0.00064
5 4 0.52 0.00110
6 4 0.52 0.00110
7 27 14.22 0.00121
8 13 4.33 0.00126
9 9 2.72 0.00127
10 166 189.49 0.00142
11 7 1.79 0.00161
12 4 0.81 0.00175
13 3 0.29 0.00176
14 4 0.64 0.00263
15 3 0.46 0.00333
16 3 0.35 0.00337
17 3 0.35 0.00337
18 10 3.18 0.00367
19 12 8.73 0.00376
20 4 0.75 0.00519
21 6 1.04 0.00557
22 3 0.40 0.00564
23 3 0.40 0.00564
24 14 8.67 0.00735
25 6 2.25 0.00858
26 178 226.02 0.00863
27 9 4.45 0.00887
28 10 4.85 0.00950
29 4 0.92 0.00957
30 3 0.46 0.00959
go_table[,-1]
Term Annotated Significant
1 extracellular matrix organization 208 39
2 collagen fibril organization 31 8
3 glycosaminoglycan catabolic process 40 9
4 collagen biosynthetic process 29 5
5 biotin metabolic process 9 4
6 positive regulation of myelination 9 4
7 ossification 246 27
8 glycosaminoglycan biosynthetic process 75 13
9 cell redox homeostasis 47 9
10 cellular protein metabolic process 3279 166
11 endodermal cell differentiation 31 7
12 left/right pattern formation 14 4
13 mesenchymal-epithelial cell signaling 5 3
14 protein heterotrimerization 11 4
15 fat-soluble vitamin biosynthetic process 8 3
16 positive regulation of keratinocyte prol... 6 3
17 postsynaptic actin cytoskeleton organiza... 6 3
18 oligodendrocyte differentiation 55 10
19 cell junction assembly 151 12
20 skeletal muscle contraction 13 4
21 regulation of SMAD protein signal transd... 18 6
22 actin crosslink formation 7 3
23 bone trabecula formation 7 3
24 osteoblast differentiation 150 14
25 cellular polysaccharide biosynthetic pro... 39 6
26 biosynthetic process 3911 178
27 odontogenesis 77 9
28 eye morphogenesis 84 10
29 extracellular matrix assembly 16 4
30 regulation of hepatocyte proliferation 8 3
Expected weightFisher
1 12.02 6.1e-06
2 1.79 0.00028
3 2.31 0.00030
4 1.68 0.00064
5 0.52 0.00110
6 0.52 0.00110
7 14.22 0.00121
8 4.33 0.00126
9 2.72 0.00127
10 189.49 0.00142
11 1.79 0.00161
12 0.81 0.00175
13 0.29 0.00176
14 0.64 0.00263
15 0.46 0.00333
16 0.35 0.00337
17 0.35 0.00337
18 3.18 0.00367
19 8.73 0.00376
20 0.75 0.00519
21 1.04 0.00557
22 0.40 0.00564
23 0.40 0.00564
24 8.67 0.00735
25 2.25 0.00858
26 226.02 0.00863
27 4.45 0.00887
28 4.85 0.00950
29 0.92 0.00957
30 0.46 0.00959
First create the gene universe for downregulated genes This is all the genes tested for differential expression assigned a 1 for differentially expressed and 0 if not.
threshold <- 0.05
gene_universe_down <- as.numeric(results[["Unstrain"]]$qvalue < threshold & results[["Unstrain"]]$logFC < 0)
gene_universe_down <- factor(gene_universe_down)
names(gene_universe_down) <- rownames(results[["Unstrain"]])
head(gene_universe_down)
ENSG00000225972 ENSG00000225630 ENSG00000237973 ENSG00000248527
0 0 0 0
ENSG00000198744 ENSG00000228794
0 0
Levels: 0 1
Create the topGO data object. Only consider “Biological Process” categories and use the Mouse Ensembl database for annotation.
go_data <- new("topGOdata",
ontology = "BP",
allGenes = gene_universe_down,
nodeSize = 5,
annotationFun = annFUN.org,
mapping = "org.Hs.eg.db",
ID = "ensembl")
Building most specific GOs .....
( 9790 GO terms found. )
Build GO DAG topology ..........
( 13875 GO terms and 32681 relations. )
Annotating nodes ...............
( 9223 genes annotated to the GO terms. )
Use the weight01 algorithm and score the tests with Fisher’s exact test.
go_test <- runTest(go_data, algorithm = "weight01", statistic = "fisher")
-- Weight01 Algorithm --
the algorithm is scoring 5175 nontrivial nodes
parameters:
test statistic: fisher
Level 19: 1 nodes to be scored (0 eliminated genes)
Level 18: 3 nodes to be scored (0 eliminated genes)
Level 17: 6 nodes to be scored (11 eliminated genes)
Level 16: 17 nodes to be scored (17 eliminated genes)
Level 15: 47 nodes to be scored (48 eliminated genes)
Level 14: 97 nodes to be scored (188 eliminated genes)
Level 13: 151 nodes to be scored (516 eliminated genes)
Level 12: 239 nodes to be scored (1339 eliminated genes)
Level 11: 385 nodes to be scored (2797 eliminated genes)
Level 10: 552 nodes to be scored (4127 eliminated genes)
Level 9: 677 nodes to be scored (5231 eliminated genes)
Level 8: 699 nodes to be scored (6639 eliminated genes)
Level 7: 800 nodes to be scored (7483 eliminated genes)
Level 6: 697 nodes to be scored (8110 eliminated genes)
Level 5: 439 nodes to be scored (8539 eliminated genes)
Level 4: 238 nodes to be scored (8808 eliminated genes)
Level 3: 105 nodes to be scored (8979 eliminated genes)
Level 2: 21 nodes to be scored (9026 eliminated genes)
Level 1: 1 nodes to be scored (9091 eliminated genes)
Keep the results with a Fisher’s exact test p-value < 0.01.
go_table <- GenTable(go_data, weightFisher = go_test,
orderBy = "weightFisher", ranksOf = "weightFisher",
topNodes = sum(score(go_test) < .01))
go_table
GO.ID Term Annotated
1 GO:0055114 oxidation-reduction process 605
2 GO:0046364 monosaccharide biosynthetic process 63
3 GO:0051085 chaperone cofactor-dependent protein ref... 11
4 GO:0006695 cholesterol biosynthetic process 56
5 GO:0030223 neutrophil differentiation 6
6 GO:1900034 regulation of cellular response to heat 73
7 GO:0006986 response to unfolded protein 141
8 GO:0036499 PERK-mediated unfolded protein response 16
9 GO:0034135 regulation of toll-like receptor 2 signa... 7
10 GO:0019321 pentose metabolic process 7
11 GO:1903364 positive regulation of cellular protein ... 108
12 GO:0045540 regulation of cholesterol biosynthetic p... 39
13 GO:0032874 positive regulation of stress-activated ... 95
14 GO:0021955 central nervous system neuron axonogenes... 25
15 GO:0050927 positive regulation of positive chemotax... 10
16 GO:0034144 negative regulation of toll-like recepto... 5
17 GO:0098712 L-glutamate import across plasma membran... 5
18 GO:0071500 cellular response to nitrosative stress 5
19 GO:0006793 phosphorus metabolic process 2036
20 GO:0030852 regulation of granulocyte differentiatio... 6
21 GO:1905323 telomerase holoenzyme complex assembly 6
22 GO:0060402 calcium ion transport into cytosol 66
23 GO:2000106 regulation of leukocyte apoptotic proces... 35
24 GO:0006098 pentose-phosphate shunt 12
25 GO:0050771 negative regulation of axonogenesis 26
26 GO:2001020 regulation of response to DNA damage sti... 157
27 GO:0002063 chondrocyte development 21
28 GO:0043066 negative regulation of apoptotic process 535
29 GO:0045104 intermediate filament cytoskeleton organ... 22
30 GO:0051131 chaperone-mediated protein complex assem... 14
31 GO:0036151 phosphatidylcholine acyl-chain remodelin... 14
32 GO:0002446 neutrophil mediated immunity 309
33 GO:0061052 negative regulation of cell growth invol... 8
34 GO:0035973 aggrephagy 8
35 GO:0090650 cellular response to oxygen-glucose depr... 8
36 GO:0048638 regulation of developmental growth 174
37 GO:0035336 long-chain fatty-acyl-CoA metabolic proc... 14
Significant Expected weightFisher
1 51 33.72 5.8e-06
2 8 3.51 9.5e-06
3 6 0.61 1.1e-05
4 16 3.12 1.7e-05
5 4 0.33 0.00013
6 13 4.07 0.00017
7 22 7.86 0.00025
8 5 0.89 0.00029
9 4 0.39 0.00029
10 4 0.39 0.00029
11 14 6.02 0.00041
12 9 2.17 0.00044
13 13 5.29 0.00093
14 5 1.39 0.00096
15 4 0.56 0.00153
16 3 0.28 0.00158
17 3 0.28 0.00158
18 3 0.28 0.00158
19 138 113.47 0.00270
20 3 0.33 0.00303
21 3 0.33 0.00303
22 9 3.68 0.00306
23 5 1.95 0.00309
24 4 0.67 0.00330
25 7 1.45 0.00448
26 18 8.75 0.00490
27 5 1.17 0.00509
28 51 29.82 0.00580
29 5 1.23 0.00607
30 4 0.78 0.00610
31 4 0.78 0.00610
32 20 17.22 0.00779
33 3 0.45 0.00781
34 3 0.45 0.00781
35 3 0.45 0.00781
36 16 9.70 0.00880
37 3 0.78 0.00894
go_table[,-1]
Term Annotated Significant
1 oxidation-reduction process 605 51
2 monosaccharide biosynthetic process 63 8
3 chaperone cofactor-dependent protein ref... 11 6
4 cholesterol biosynthetic process 56 16
5 neutrophil differentiation 6 4
6 regulation of cellular response to heat 73 13
7 response to unfolded protein 141 22
8 PERK-mediated unfolded protein response 16 5
9 regulation of toll-like receptor 2 signa... 7 4
10 pentose metabolic process 7 4
11 positive regulation of cellular protein ... 108 14
12 regulation of cholesterol biosynthetic p... 39 9
13 positive regulation of stress-activated ... 95 13
14 central nervous system neuron axonogenes... 25 5
15 positive regulation of positive chemotax... 10 4
16 negative regulation of toll-like recepto... 5 3
17 L-glutamate import across plasma membran... 5 3
18 cellular response to nitrosative stress 5 3
19 phosphorus metabolic process 2036 138
20 regulation of granulocyte differentiatio... 6 3
21 telomerase holoenzyme complex assembly 6 3
22 calcium ion transport into cytosol 66 9
23 regulation of leukocyte apoptotic proces... 35 5
24 pentose-phosphate shunt 12 4
25 negative regulation of axonogenesis 26 7
26 regulation of response to DNA damage sti... 157 18
27 chondrocyte development 21 5
28 negative regulation of apoptotic process 535 51
29 intermediate filament cytoskeleton organ... 22 5
30 chaperone-mediated protein complex assem... 14 4
31 phosphatidylcholine acyl-chain remodelin... 14 4
32 neutrophil mediated immunity 309 20
33 negative regulation of cell growth invol... 8 3
34 aggrephagy 8 3
35 cellular response to oxygen-glucose depr... 8 3
36 regulation of developmental growth 174 16
37 long-chain fatty-acyl-CoA metabolic proc... 14 3
Expected weightFisher
1 33.72 5.8e-06
2 3.51 9.5e-06
3 0.61 1.1e-05
4 3.12 1.7e-05
5 0.33 0.00013
6 4.07 0.00017
7 7.86 0.00025
8 0.89 0.00029
9 0.39 0.00029
10 0.39 0.00029
11 6.02 0.00041
12 2.17 0.00044
13 5.29 0.00093
14 1.39 0.00096
15 0.56 0.00153
16 0.28 0.00158
17 0.28 0.00158
18 0.28 0.00158
19 113.47 0.00270
20 0.33 0.00303
21 0.33 0.00303
22 3.68 0.00306
23 1.95 0.00309
24 0.67 0.00330
25 1.45 0.00448
26 8.75 0.00490
27 1.17 0.00509
28 29.82 0.00580
29 1.23 0.00607
30 0.78 0.00610
31 0.78 0.00610
32 17.22 0.00779
33 0.45 0.00781
34 0.45 0.00781
35 0.45 0.00781
36 9.70 0.00880
37 0.78 0.00894
single_filt_counts <- filt_counts
anno <- pData(RUVsOut)
NA18855_index <- which(anno$Individual == "NA18855 ")
NA18855 <- single_filt_counts[, NA18855_index]
NA18855_anno <- anno[NA18855_index, ]
NA18856_index <- which(anno$Individual == "NA18856 ")
NA18856 <- single_filt_counts[, NA18856_index]
NA18856_anno <- anno[NA18856_index, ]
NA19160_index <- which(anno$Individual == "NA19160 ")
NA19160 <- single_filt_counts[, NA19160_index]
NA19160_anno <- anno[NA19160_index, ]
plot_volcano <- function(x, qval) {
# Create volcano plot.
#
# x - data frame with topTable and ASH output
# (columns logFC, P.Value, and qvalue)
# qval - qvalue cutoff for calling a gene DE
#
stopifnot(is.data.frame(x), c("logFC", "P.Value", "qvalue") %in% colnames(x),
is.numeric(qval), qval <= 1, qval >= 0)
x$highlight <- ifelse(x$qvalue < qval, "darkred", "gray75")
x$highlight <- factor(x$highlight, levels = c("darkred", "gray75"))
ggplot(x, aes(x = logFC, y = -log10(P.Value), color = highlight)) +
geom_point(shape = 1) +
labs(x = "Log fold change",
y = expression(-log[10] * " p-value")) +
scale_color_identity(drop = FALSE) +
theme(legend.position = "none")
}
volcano_plot_ind <- function(annotation, filtered_counts){
design <- model.matrix(~treatment + W_1 + W_2 + RIN,
data = annotation)
colnames(design) <- gsub("treatment", "", colnames(design))
#TMM Normalization
y <- DGEList(filtered_counts)
y <- calcNormFactors(y, method = "TMM")
#Voom for differential expression
v1 <- voom(y, design)
fit <- lmFit(v1, design, block = annotation$treatment,
correlation = corfit2$consensus.correlation)
fit <- eBayes(fit)
#extract results and run ash
tests <- colnames(fit$coefficients)
results <- vector(length = length(tests), mode = "list")
names(results) <- tests
for (test in tests) {
# Extract limma results
results[[test]] <- get_results(fit, coef = test)
# Add mutliple testing correction with ASH
output_ash <- run_ash(fit, coef = test)$result
results[[test]] <- cbind(results[[test]], lfsr = output_ash$lfsr,
lfdr = output_ash$lfdr, qvalue = output_ash$qvalue,
svalue = output_ash$svalue)
}
#FDR 0.05
print(plot_volcano(results[["Unstrain"]], 0.05))
print(table(results[["Unstrain"]]$logFC > 0))
print(table(results[["Unstrain"]]$logFC > 0.25))
print(table(results[["Unstrain"]]$logFC < -0.25))
}
#make plots
volcano_plot_ind(NA18855_anno, NA18855)

FALSE TRUE
5229 5288
FALSE TRUE
9478 1039
FALSE TRUE
9591 926
volcano_plot_ind(NA18856_anno, NA18856)

FALSE TRUE
5566 4951
FALSE TRUE
7792 2725
FALSE TRUE
7515 3002
#volcano_plot_ind(NA19160_anno, NA19160)
#19160
design <- model.matrix(~treatment + W_1 + W_2,
data = NA19160_anno)
colnames(design) <- gsub("treatment", "", colnames(design))
#TMM Normalization
y <- DGEList(NA19160)
y <- calcNormFactors(y, method = "TMM")
#Voom for differential expression
v1 <- voom(y, design)
fit <- lmFit(v1, design, block = NA19160_anno$treatment,
correlation = corfit2$consensus.correlation)
fit <- eBayes(fit)
#extract results and run ash
tests <- colnames(fit$coefficients)
results <- vector(length = length(tests), mode = "list")
names(results) <- tests
for (test in tests) {
# Extract limma results
results[[test]] <- get_results(fit, coef = test)
# Add mutliple testing correction with ASH
output_ash <- run_ash(fit, coef = test)$result
results[[test]] <- cbind(results[[test]], lfsr = output_ash$lfsr,
lfdr = output_ash$lfdr, qvalue = output_ash$qvalue,
svalue = output_ash$svalue)
}
#FDR 0.05
plot_volcano(results[["Unstrain"]], 0.05)

table(results[["Unstrain"]]$logFC > 0)
FALSE TRUE
5309 5208
table(results[["Unstrain"]]$logFC > 0.25)
FALSE TRUE
9458 1059
table(results[["Unstrain"]]$logFC < -0.25)
FALSE TRUE
9530 987
From Ben Fair: “i have a suggestion for an analysis as a check that all your DE results (like the GO analysis, the enrichment for DE genes from published datasets) are robust… i’m not sure this is statistically perfect but its something that I think makes sense to try, for my own sanity: if you randomly reassign control and treatment labels and re-attampt DE testing, do all of those interesting results go away? the worry i am trying to address, is that you have some enrichment of DE genes in interesting GO categories for example, but is that just because you have more DE power for detecting highly expressed genes in this cell type (even if they are only modestly or not truly DE), and that is what drives the GO enrichment.”
#scramble treatment labels (x3) and store the DE genes (each time, randomly assign 9 samples to be strain)
# permuted_results <- vector("list", 12)
# for(time in 1:6){
# #set up DE and scramble labels
# anno <- pData(RUVsOut)
# x <- paste0(anno$Individual, anno$treatment)
#
# anno$LibraryPrepBatch <- factor(anno$LibraryPrepBatch, levels = c("1", "2"))
# anno$Replicate <- factor(anno$Replicate, levels = c("1", "2", "3"))
#
# strain_indices <- sample(nrow(anno), 9)
# anno$treatment <- "Unstrain"
# anno$treatment[strain_indices] <- "Strain"
# design <- model.matrix(~treatment + Sex + Individual + W_1 + W_2 + RIN,
# data = anno)
# colnames(design) <- gsub("treatment", "", colnames(design))
#
# permuted_results[[time]] <- anno
#
# #perform DE
# #TMM Normalization
# y <- DGEList(single_filt_counts)
# y <- calcNormFactors(y, method = "TMM")
# #Voom for differential expression
# v1 <- voom(y, design)
# corfit1 <- duplicateCorrelation(v1, design, block = x)
# corfit1$consensus.correlation
# v2 <- voom(y, design, block = x, correlation = corfit1$consensus.correlation)
# corfit2 <- duplicateCorrelation(v2, design, block = x)
# corfit2$consensus.correlation
# fit <- lmFit(v2, design, block = x,
# correlation = corfit2$consensus.correlation)
# fit <- eBayes(fit)
#
# tests <- colnames(fit$coefficients)
# results <- vector(length = length(tests), mode = "list")
# names(results) <- tests
#
# for (test in tests[c(1:4, 6:8)]) {
# # Extract limma results
# results[[test]] <- get_results(fit, coef = test)
# # Add mutliple testing correction with ASH
# output_ash <- run_ash(fit, coef = test)$result
# results[[test]] <- cbind(results[[test]], lfsr = output_ash$lfsr,
# lfdr = output_ash$lfdr, qvalue = output_ash$qvalue,
# svalue = output_ash$svalue)
# }
#
# #store results
# permuted_results[[6+time]] <- results[["Unstrain"]]
# }
# saveRDS(permuted_results, "output/permuted_results_DE.rds")
permuted_results <- readRDS("output/permuted_results_DE.rds")
permuted_results
[[1]]
Sample_ID Individual Sex Replicate treatment RIN
18855_3_S 18855_3_S NA18855 F 3 Unstrain 10.0
19160_3_S 19160_3_S NA19160 M 3 Strain 10.0
18856_3_U 18856_3_U NA18856 M 3 Unstrain 10.0
18856_1_U 18856_1_U NA18856 M 1 Unstrain 9.9
18855_2_S 18855_2_S NA18855 F 2 Strain 10.0
18856_2_S 18856_2_S NA18856 M 2 Unstrain 9.6
19160_3_U 19160_3_U NA19160 M 3 Unstrain 10.0
18855_2_U 18855_2_U NA18855 F 2 Strain 10.0
19160_2_S 19160_2_S NA19160 M 2 Strain 10.0
18855_1_S 18855_1_S NA18855 F 1 Unstrain 9.9
18856_1_S 18856_1_S NA18856 M 1 Unstrain 8.5
19160_1_S 19160_1_S NA19160 M 1 Strain 9.9
19160_2_U 19160_2_U NA19160 M 2 Strain 10.0
18855_1_U 18855_1_U NA18855 F 1 Strain 10.0
18856_3_S 18856_3_S NA18856 M 3 Strain 9.6
18856_2_U 18856_2_U NA18856 M 2 Strain 9.5
18855_3_U 18855_3_U NA18855 F 3 Unstrain 10.0
LibraryPrepBatch LibSize W_1 W_2
18855_3_S 2 15419569 0.6403178 -1.0334196
19160_3_S 2 15177653 0.4887528 -1.3355831
18856_3_U 2 13722909 0.9502388 -0.7892404
18856_1_U 1 18647599 1.2228123 -1.4496449
18855_2_S 1 18256163 0.8233400 -0.6748150
18856_2_S 1 16782764 0.0515988 -1.2784836
19160_3_U 2 19839604 0.3406102 -1.4859733
18855_2_U 1 18618691 0.7609177 -0.8596946
19160_2_S 1 16622407 0.4247172 -1.4414851
18855_1_S 1 19712744 0.7356719 -1.4499412
18856_1_S 1 13571439 1.1409773 -1.1686123
19160_1_S 1 20979958 0.5909682 -1.9538068
19160_2_U 1 20924281 0.1295380 -1.8537818
18855_1_U 1 20146697 0.7569979 -1.3228202
18856_3_S 2 15807863 0.8205467 -0.8981659
18856_2_U 1 15142152 0.4572116 -1.0041098
18855_3_U 2 18157918 0.5909283 -1.1455295
[[2]]
Sample_ID Individual Sex Replicate treatment RIN
18855_3_S 18855_3_S NA18855 F 3 Strain 10.0
19160_3_S 19160_3_S NA19160 M 3 Strain 10.0
18856_3_U 18856_3_U NA18856 M 3 Unstrain 10.0
18856_1_U 18856_1_U NA18856 M 1 Unstrain 9.9
18855_2_S 18855_2_S NA18855 F 2 Unstrain 10.0
18856_2_S 18856_2_S NA18856 M 2 Strain 9.6
19160_3_U 19160_3_U NA19160 M 3 Strain 10.0
18855_2_U 18855_2_U NA18855 F 2 Strain 10.0
19160_2_S 19160_2_S NA19160 M 2 Strain 10.0
18855_1_S 18855_1_S NA18855 F 1 Strain 9.9
18856_1_S 18856_1_S NA18856 M 1 Strain 8.5
19160_1_S 19160_1_S NA19160 M 1 Unstrain 9.9
19160_2_U 19160_2_U NA19160 M 2 Unstrain 10.0
18855_1_U 18855_1_U NA18855 F 1 Strain 10.0
18856_3_S 18856_3_S NA18856 M 3 Unstrain 9.6
18856_2_U 18856_2_U NA18856 M 2 Unstrain 9.5
18855_3_U 18855_3_U NA18855 F 3 Unstrain 10.0
LibraryPrepBatch LibSize W_1 W_2
18855_3_S 2 15419569 0.6403178 -1.0334196
19160_3_S 2 15177653 0.4887528 -1.3355831
18856_3_U 2 13722909 0.9502388 -0.7892404
18856_1_U 1 18647599 1.2228123 -1.4496449
18855_2_S 1 18256163 0.8233400 -0.6748150
18856_2_S 1 16782764 0.0515988 -1.2784836
19160_3_U 2 19839604 0.3406102 -1.4859733
18855_2_U 1 18618691 0.7609177 -0.8596946
19160_2_S 1 16622407 0.4247172 -1.4414851
18855_1_S 1 19712744 0.7356719 -1.4499412
18856_1_S 1 13571439 1.1409773 -1.1686123
19160_1_S 1 20979958 0.5909682 -1.9538068
19160_2_U 1 20924281 0.1295380 -1.8537818
18855_1_U 1 20146697 0.7569979 -1.3228202
18856_3_S 2 15807863 0.8205467 -0.8981659
18856_2_U 1 15142152 0.4572116 -1.0041098
18855_3_U 2 18157918 0.5909283 -1.1455295
[[3]]
Sample_ID Individual Sex Replicate treatment RIN
18855_3_S 18855_3_S NA18855 F 3 Strain 10.0
19160_3_S 19160_3_S NA19160 M 3 Strain 10.0
18856_3_U 18856_3_U NA18856 M 3 Strain 10.0
18856_1_U 18856_1_U NA18856 M 1 Strain 9.9
18855_2_S 18855_2_S NA18855 F 2 Unstrain 10.0
18856_2_S 18856_2_S NA18856 M 2 Unstrain 9.6
19160_3_U 19160_3_U NA19160 M 3 Unstrain 10.0
18855_2_U 18855_2_U NA18855 F 2 Strain 10.0
19160_2_S 19160_2_S NA19160 M 2 Unstrain 10.0
18855_1_S 18855_1_S NA18855 F 1 Strain 9.9
18856_1_S 18856_1_S NA18856 M 1 Strain 8.5
19160_1_S 19160_1_S NA19160 M 1 Strain 9.9
19160_2_U 19160_2_U NA19160 M 2 Unstrain 10.0
18855_1_U 18855_1_U NA18855 F 1 Unstrain 10.0
18856_3_S 18856_3_S NA18856 M 3 Unstrain 9.6
18856_2_U 18856_2_U NA18856 M 2 Unstrain 9.5
18855_3_U 18855_3_U NA18855 F 3 Strain 10.0
LibraryPrepBatch LibSize W_1 W_2
18855_3_S 2 15419569 0.6403178 -1.0334196
19160_3_S 2 15177653 0.4887528 -1.3355831
18856_3_U 2 13722909 0.9502388 -0.7892404
18856_1_U 1 18647599 1.2228123 -1.4496449
18855_2_S 1 18256163 0.8233400 -0.6748150
18856_2_S 1 16782764 0.0515988 -1.2784836
19160_3_U 2 19839604 0.3406102 -1.4859733
18855_2_U 1 18618691 0.7609177 -0.8596946
19160_2_S 1 16622407 0.4247172 -1.4414851
18855_1_S 1 19712744 0.7356719 -1.4499412
18856_1_S 1 13571439 1.1409773 -1.1686123
19160_1_S 1 20979958 0.5909682 -1.9538068
19160_2_U 1 20924281 0.1295380 -1.8537818
18855_1_U 1 20146697 0.7569979 -1.3228202
18856_3_S 2 15807863 0.8205467 -0.8981659
18856_2_U 1 15142152 0.4572116 -1.0041098
18855_3_U 2 18157918 0.5909283 -1.1455295
[[4]]
Sample_ID Individual Sex Replicate treatment RIN
18855_3_S 18855_3_S NA18855 F 3 Unstrain 10.0
19160_3_S 19160_3_S NA19160 M 3 Unstrain 10.0
18856_3_U 18856_3_U NA18856 M 3 Unstrain 10.0
18856_1_U 18856_1_U NA18856 M 1 Unstrain 9.9
18855_2_S 18855_2_S NA18855 F 2 Strain 10.0
18856_2_S 18856_2_S NA18856 M 2 Unstrain 9.6
19160_3_U 19160_3_U NA19160 M 3 Strain 10.0
18855_2_U 18855_2_U NA18855 F 2 Strain 10.0
19160_2_S 19160_2_S NA19160 M 2 Strain 10.0
18855_1_S 18855_1_S NA18855 F 1 Strain 9.9
18856_1_S 18856_1_S NA18856 M 1 Strain 8.5
19160_1_S 19160_1_S NA19160 M 1 Unstrain 9.9
19160_2_U 19160_2_U NA19160 M 2 Unstrain 10.0
18855_1_U 18855_1_U NA18855 F 1 Strain 10.0
18856_3_S 18856_3_S NA18856 M 3 Strain 9.6
18856_2_U 18856_2_U NA18856 M 2 Unstrain 9.5
18855_3_U 18855_3_U NA18855 F 3 Strain 10.0
LibraryPrepBatch LibSize W_1 W_2
18855_3_S 2 15419569 0.6403178 -1.0334196
19160_3_S 2 15177653 0.4887528 -1.3355831
18856_3_U 2 13722909 0.9502388 -0.7892404
18856_1_U 1 18647599 1.2228123 -1.4496449
18855_2_S 1 18256163 0.8233400 -0.6748150
18856_2_S 1 16782764 0.0515988 -1.2784836
19160_3_U 2 19839604 0.3406102 -1.4859733
18855_2_U 1 18618691 0.7609177 -0.8596946
19160_2_S 1 16622407 0.4247172 -1.4414851
18855_1_S 1 19712744 0.7356719 -1.4499412
18856_1_S 1 13571439 1.1409773 -1.1686123
19160_1_S 1 20979958 0.5909682 -1.9538068
19160_2_U 1 20924281 0.1295380 -1.8537818
18855_1_U 1 20146697 0.7569979 -1.3228202
18856_3_S 2 15807863 0.8205467 -0.8981659
18856_2_U 1 15142152 0.4572116 -1.0041098
18855_3_U 2 18157918 0.5909283 -1.1455295
[[5]]
Sample_ID Individual Sex Replicate treatment RIN
18855_3_S 18855_3_S NA18855 F 3 Unstrain 10.0
19160_3_S 19160_3_S NA19160 M 3 Unstrain 10.0
18856_3_U 18856_3_U NA18856 M 3 Unstrain 10.0
18856_1_U 18856_1_U NA18856 M 1 Unstrain 9.9
18855_2_S 18855_2_S NA18855 F 2 Unstrain 10.0
18856_2_S 18856_2_S NA18856 M 2 Strain 9.6
19160_3_U 19160_3_U NA19160 M 3 Strain 10.0
18855_2_U 18855_2_U NA18855 F 2 Unstrain 10.0
19160_2_S 19160_2_S NA19160 M 2 Strain 10.0
18855_1_S 18855_1_S NA18855 F 1 Unstrain 9.9
18856_1_S 18856_1_S NA18856 M 1 Unstrain 8.5
19160_1_S 19160_1_S NA19160 M 1 Strain 9.9
19160_2_U 19160_2_U NA19160 M 2 Strain 10.0
18855_1_U 18855_1_U NA18855 F 1 Strain 10.0
18856_3_S 18856_3_S NA18856 M 3 Strain 9.6
18856_2_U 18856_2_U NA18856 M 2 Strain 9.5
18855_3_U 18855_3_U NA18855 F 3 Strain 10.0
LibraryPrepBatch LibSize W_1 W_2
18855_3_S 2 15419569 0.6403178 -1.0334196
19160_3_S 2 15177653 0.4887528 -1.3355831
18856_3_U 2 13722909 0.9502388 -0.7892404
18856_1_U 1 18647599 1.2228123 -1.4496449
18855_2_S 1 18256163 0.8233400 -0.6748150
18856_2_S 1 16782764 0.0515988 -1.2784836
19160_3_U 2 19839604 0.3406102 -1.4859733
18855_2_U 1 18618691 0.7609177 -0.8596946
19160_2_S 1 16622407 0.4247172 -1.4414851
18855_1_S 1 19712744 0.7356719 -1.4499412
18856_1_S 1 13571439 1.1409773 -1.1686123
19160_1_S 1 20979958 0.5909682 -1.9538068
19160_2_U 1 20924281 0.1295380 -1.8537818
18855_1_U 1 20146697 0.7569979 -1.3228202
18856_3_S 2 15807863 0.8205467 -0.8981659
18856_2_U 1 15142152 0.4572116 -1.0041098
18855_3_U 2 18157918 0.5909283 -1.1455295
[[6]]
Sample_ID Individual Sex Replicate treatment RIN
18855_3_S 18855_3_S NA18855 F 3 Unstrain 10.0
19160_3_S 19160_3_S NA19160 M 3 Strain 10.0
18856_3_U 18856_3_U NA18856 M 3 Unstrain 10.0
18856_1_U 18856_1_U NA18856 M 1 Unstrain 9.9
18855_2_S 18855_2_S NA18855 F 2 Unstrain 10.0
18856_2_S 18856_2_S NA18856 M 2 Strain 9.6
19160_3_U 19160_3_U NA19160 M 3 Strain 10.0
18855_2_U 18855_2_U NA18855 F 2 Unstrain 10.0
19160_2_S 19160_2_S NA19160 M 2 Strain 10.0
18855_1_S 18855_1_S NA18855 F 1 Unstrain 9.9
18856_1_S 18856_1_S NA18856 M 1 Strain 8.5
19160_1_S 19160_1_S NA19160 M 1 Strain 9.9
19160_2_U 19160_2_U NA19160 M 2 Strain 10.0
18855_1_U 18855_1_U NA18855 F 1 Unstrain 10.0
18856_3_S 18856_3_S NA18856 M 3 Strain 9.6
18856_2_U 18856_2_U NA18856 M 2 Unstrain 9.5
18855_3_U 18855_3_U NA18855 F 3 Strain 10.0
LibraryPrepBatch LibSize W_1 W_2
18855_3_S 2 15419569 0.6403178 -1.0334196
19160_3_S 2 15177653 0.4887528 -1.3355831
18856_3_U 2 13722909 0.9502388 -0.7892404
18856_1_U 1 18647599 1.2228123 -1.4496449
18855_2_S 1 18256163 0.8233400 -0.6748150
18856_2_S 1 16782764 0.0515988 -1.2784836
19160_3_U 2 19839604 0.3406102 -1.4859733
18855_2_U 1 18618691 0.7609177 -0.8596946
19160_2_S 1 16622407 0.4247172 -1.4414851
18855_1_S 1 19712744 0.7356719 -1.4499412
18856_1_S 1 13571439 1.1409773 -1.1686123
19160_1_S 1 20979958 0.5909682 -1.9538068
19160_2_U 1 20924281 0.1295380 -1.8537818
18855_1_U 1 20146697 0.7569979 -1.3228202
18856_3_S 2 15807863 0.8205467 -0.8981659
18856_2_U 1 15142152 0.4572116 -1.0041098
18855_3_U 2 18157918 0.5909283 -1.1455295
[[7]]
logFC AveExpr t P.Value
ENSG00000225972 -6.767991e-01 3.439734306 -6.034212e+00 1.534683e-05
ENSG00000225630 -3.459833e-01 5.259763521 -4.409907e+00 4.107725e-04
ENSG00000237973 -2.340504e-01 5.765427761 -2.309679e+00 3.416705e-02
ENSG00000248527 -4.122446e-01 7.291375670 -5.647351e+00 3.262286e-05
ENSG00000198744 -6.027311e-02 1.692163426 -4.425191e-01 6.638701e-01
ENSG00000228794 -9.625710e-03 2.429990051 -6.846823e-02 9.462373e-01
ENSG00000223764 2.363846e-02 1.604113326 1.326653e-01 8.960655e-01
ENSG00000187634 1.311432e-01 2.774428967 1.057727e+00 3.054482e-01
ENSG00000188976 -1.271284e-01 5.602324306 -2.108812e+00 5.059980e-02
ENSG00000187961 1.325594e-01 3.205023751 1.151909e+00 2.658059e-01
ENSG00000187608 -1.051588e-01 3.515541854 -7.393004e-01 4.701314e-01
ENSG00000188157 -1.687196e-01 8.438003633 -1.784618e+00 9.275392e-02
ENSG00000131591 -4.326563e-02 2.698849622 -3.570313e-01 7.256019e-01
ENSG00000078808 -4.598536e-02 8.404430472 -6.825496e-01 5.043794e-01
ENSG00000176022 -8.386179e-03 5.315643527 -9.096719e-02 9.286159e-01
ENSG00000160087 -5.342404e-02 5.574117382 -9.565097e-01 3.526456e-01
ENSG00000131584 -1.248551e-01 5.338644789 -1.663437e+00 1.151346e-01
ENSG00000169972 -1.044143e-01 3.649507997 -1.293766e+00 2.135966e-01
ENSG00000127054 -8.861510e-02 5.813712471 -1.763807e+00 9.630361e-02
ENSG00000224051 -6.153466e-02 4.433660580 -7.176686e-01 4.830178e-01
ENSG00000107404 -9.239766e-02 5.541314411 -1.051471e+00 3.082262e-01
ENSG00000162576 -6.629820e-02 5.643016003 -6.556034e-01 5.211330e-01
ENSG00000175756 -2.330514e-02 5.949578662 -4.729930e-01 6.424261e-01
ENSG00000221978 1.246176e-01 6.117640537 1.075522e+00 2.976451e-01
ENSG00000224870 -5.212137e-02 4.193043833 -5.941803e-01 5.604655e-01
ENSG00000242485 1.097271e-02 5.474356677 1.767409e-01 8.618671e-01
ENSG00000160072 -2.606481e-02 4.020742816 -3.344057e-01 7.422941e-01
ENSG00000197785 -3.986811e-02 4.667370310 -4.901177e-01 6.305156e-01
ENSG00000160075 2.570885e-02 6.698987873 5.165866e-01 6.123117e-01
ENSG00000228594 5.667969e-02 3.231082811 4.613645e-01 6.505719e-01
ENSG00000197530 -1.670145e-01 6.011139637 -1.761902e+00 9.663438e-02
ENSG00000189409 -3.120851e-02 4.590321361 -2.866829e-01 7.779303e-01
ENSG00000248333 -2.539609e-01 3.904469734 -3.419798e+00 3.390157e-03
ENSG00000189339 -7.889013e-02 5.329578573 -1.410645e+00 1.769582e-01
ENSG00000008130 -3.467566e-02 5.472226379 -6.601556e-01 5.182809e-01
ENSG00000078369 -1.471033e-02 8.804321589 -3.445650e-01 7.347820e-01
ENSG00000162585 -9.614789e-02 4.754428869 -1.030171e+00 3.178221e-01
ENSG00000157933 -4.170617e-02 6.562145698 -7.895748e-01 4.409961e-01
ENSG00000157916 -2.380030e-02 7.002652621 -5.097098e-01 6.170168e-01
ENSG00000157911 -3.042208e-02 3.826112744 -2.880426e-01 7.769075e-01
ENSG00000157881 -1.948416e-02 4.048444809 -2.771334e-01 7.851259e-01
ENSG00000157870 -1.437396e-01 3.237377913 -1.318839e+00 2.052679e-01
ENSG00000158109 -8.364271e-02 6.732297019 -1.675515e+00 1.127121e-01
ENSG00000116213 -5.916146e-02 4.048572327 -8.210028e-01 4.233698e-01
ENSG00000227372 1.980463e-02 3.931432132 2.682032e-01 7.918732e-01
ENSG00000130764 -5.846816e-02 5.498222486 -9.254604e-01 3.680892e-01
ENSG00000116198 -6.409805e-02 4.799410870 -9.560501e-01 3.528709e-01
ENSG00000198912 1.108725e-02 4.517142545 1.609528e-01 8.740883e-01
ENSG00000131697 2.015576e-02 3.217207294 2.340496e-01 8.178311e-01
ENSG00000116251 1.021006e-01 6.491595851 1.685462e+00 1.107501e-01
ENSG00000116237 -1.106385e-02 7.144503644 -2.259333e-01 8.240333e-01
ENSG00000158292 -6.737194e-02 2.850382649 -5.631747e-01 5.809029e-01
ENSG00000097021 -3.972663e-02 4.963811963 -2.813111e-01 7.819755e-01
ENSG00000215788 -4.169332e-02 2.224832337 -2.081042e-01 8.376993e-01
ENSG00000171680 -4.813704e-02 4.816276482 -3.674191e-01 7.179852e-01
ENSG00000162408 1.623993e-01 3.954485497 1.817428e+00 8.739087e-02
ENSG00000204859 -4.964338e-02 3.319571732 -4.614250e-01 6.505294e-01
ENSG00000162413 -8.689368e-02 6.835844141 -1.626095e+00 1.229090e-01
ENSG00000116273 4.720304e-02 5.018894856 6.256304e-01 5.401309e-01
ENSG00000041988 9.402484e-02 3.387269004 1.034978e+00 3.156378e-01
ENSG00000007923 -1.393499e-01 5.116929638 -1.903423e+00 7.461167e-02
ENSG00000171735 1.790563e-02 3.971476593 1.489784e-01 8.833796e-01
ENSG00000049245 7.444088e-02 6.747961733 1.702935e+00 1.073750e-01
ENSG00000116288 -6.315667e-02 7.469041111 -1.303922e+00 2.101914e-01
ENSG00000116285 -3.437070e-02 6.567378923 -2.050315e-01 8.400601e-01
ENSG00000142599 -3.149498e-01 6.882232369 -5.493637e+00 4.425887e-05
ENSG00000074800 7.995019e-03 9.240103588 1.314682e-01 8.969975e-01
ENSG00000142583 8.556524e-02 2.154021950 4.111849e-01 6.862377e-01
ENSG00000180758 1.316956e-01 2.520571933 1.375868e+00 1.872857e-01
ENSG00000228526 -1.147713e-01 3.111024724 -6.291662e-01 5.378702e-01
ENSG00000049239 4.506518e-02 5.843277506 5.021609e-01 6.222015e-01
ENSG00000171621 6.160367e-02 7.640791271 7.922669e-01 4.394685e-01
ENSG00000171612 1.213680e-01 2.962339532 1.017418e+00 3.236687e-01
ENSG00000188807 5.328492e-03 3.161234025 5.232693e-02 9.588976e-01
ENSG00000171608 7.094923e-02 3.600417471 6.282114e-01 5.384802e-01
ENSG00000171603 3.306178e-02 7.507456408 5.380248e-01 5.977563e-01
ENSG00000178585 6.346380e-02 4.096458428 7.955617e-01 4.376034e-01
ENSG00000162441 1.186006e-01 4.190251149 1.529293e+00 1.451704e-01
ENSG00000173614 2.975761e-02 3.790237230 2.848469e-01 7.793121e-01
ENSG00000130939 -6.197711e-02 6.013665524 -1.037435e+00 3.145257e-01
ENSG00000054523 -4.123988e-02 6.790885629 -8.603956e-01 4.019228e-01
ENSG00000142657 -3.729739e-03 6.764910548 -7.057029e-02 9.445896e-01
ENSG00000160049 1.313065e-02 5.436360887 2.673031e-01 7.925542e-01
ENSG00000142655 -8.607047e-02 3.921860299 -1.144931e+00 2.686041e-01
ENSG00000120948 4.740066e-03 5.498744258 8.865993e-02 9.304213e-01
ENSG00000116649 -1.282801e-01 6.989287261 -1.721375e+00 1.039100e-01
ENSG00000171824 -7.211280e-02 5.208139078 -1.244451e+00 2.307527e-01
ENSG00000198793 -9.718115e-02 6.572268484 -1.652002e+00 1.174692e-01
ENSG00000171819 -2.522350e-02 5.005029463 -1.376544e-01 8.921824e-01
ENSG00000120942 -4.665394e-02 4.505427589 -6.996204e-01 4.939283e-01
ENSG00000116661 4.538212e-02 2.256655281 2.892704e-01 7.759842e-01
ENSG00000132879 5.047387e-02 2.851964046 5.140595e-01 6.140387e-01
ENSG00000116670 -1.620555e-02 3.776766362 -1.788251e-01 8.602564e-01
ENSG00000177674 -1.650027e-02 4.748151475 -2.329628e-01 8.186609e-01
ENSG00000177000 -1.954206e-02 4.306535766 -2.608867e-01 7.974140e-01
ENSG00000011021 -5.368589e-02 4.716053644 -7.572647e-01 4.595891e-01
ENSG00000116685 -2.715616e-02 5.360029702 -4.072493e-01 6.890691e-01
ENSG00000083444 -3.196610e-04 9.617689757 -4.644060e-03 9.963504e-01
ENSG00000116688 -2.886059e-02 6.632063082 -5.603383e-01 5.827914e-01
ENSG00000116691 -9.442851e-02 3.486834952 -1.371627e+00 1.885779e-01
ENSG00000048707 -4.387627e-02 5.830304323 -7.811816e-01 4.457803e-01
ENSG00000162496 7.132749e-03 3.119770104 4.998909e-02 9.607323e-01
ENSG00000162494 1.108371e-01 1.522400198 6.695634e-01 5.124145e-01
ENSG00000162493 6.301397e-02 9.669928769 9.404883e-01 3.605578e-01
ENSG00000116731 9.616667e-03 5.589398806 1.860587e-01 8.546711e-01
ENSG00000189337 -3.245446e-01 3.000052029 -2.734260e+00 1.442174e-02
ENSG00000171729 -1.172006e-02 3.118331547 -7.152052e-02 9.438448e-01
ENSG00000142634 -1.343972e-01 4.068304279 -1.177448e+00 2.557526e-01
ENSG00000132906 2.680318e-02 3.641839894 3.054571e-01 7.638449e-01
ENSG00000116138 7.087244e-02 5.209789620 1.186077e+00 2.524217e-01
ENSG00000197312 3.885225e-02 5.118656021 5.126870e-01 6.149777e-01
ENSG00000116786 -5.266448e-02 6.306994840 -1.100670e+00 2.868677e-01
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ENSG00000126461 -1.997334e-01 6.133402435 -2.058226e+00 5.575231e-02
ENSG00000126456 -1.403812e-01 4.969957329 -2.268460e+00 3.706882e-02
ENSG00000126453 -2.204106e-01 2.842914353 -2.127428e+00 4.881585e-02
ENSG00000126457 -1.421547e-01 6.324574391 -2.581586e+00 1.975019e-02
ENSG00000196961 -1.039950e-01 6.862993870 -1.465106e+00 1.617213e-01
ENSG00000010361 1.053325e-01 2.746988061 8.052597e-01 4.321424e-01
ENSG00000104973 -2.247663e-01 4.644685303 -2.719239e+00 1.487751e-02
ENSG00000104960 1.434868e-02 5.126602512 2.068590e-01 8.386558e-01
ENSG00000039650 -6.621613e-02 4.318555072 -8.696405e-01 3.969945e-01
ENSG00000204673 -1.025748e-01 6.615684364 -1.457379e+00 1.638150e-01
ENSG00000104946 -8.609590e-02 5.142717077 -1.172642e+00 2.576221e-01
ENSG00000213024 1.202328e-02 5.178261322 2.132699e-01 8.337341e-01
ENSG00000169136 -9.710737e-02 3.340949893 -1.043520e+00 3.117835e-01
ENSG00000105053 1.229434e-01 4.303539969 1.380423e+00 1.859060e-01
ENSG00000142528 5.598441e-02 3.054946621 5.363498e-01 5.988873e-01
ENSG00000131408 -1.885685e-02 5.748430698 -2.472178e-01 8.077951e-01
ENSG00000062822 -3.852277e-02 3.000646471 -3.341149e-01 7.425095e-01
ENSG00000161671 -5.843566e-02 6.833897974 -8.781085e-01 3.925153e-01
ENSG00000161677 -6.169624e-02 4.863914065 -7.738741e-01 4.499718e-01
ENSG00000167747 1.569852e-02 4.345002868 1.868997e-01 8.540222e-01
ENSG00000142544 6.314276e-02 2.311663930 2.972649e-01 7.699810e-01
ENSG00000105379 -1.055500e-01 3.407808788 -9.624280e-01 3.497534e-01
ENSG00000105497 7.406711e-02 3.196139230 8.982416e-01 3.820010e-01
ENSG00000182310 2.020385e-02 2.485832085 1.722401e-01 8.653476e-01
ENSG00000198093 -1.781771e-01 2.053797321 -1.324042e+00 2.035723e-01
ENSG00000176024 1.229927e-01 2.592669391 1.236849e+00 2.334902e-01
ENSG00000256683 -3.022781e-02 2.455207926 -2.551277e-01 8.017831e-01
ENSG00000197619 3.954679e-01 2.482955439 2.791343e+00 1.280924e-02
ENSG00000142556 1.259079e-01 3.382865635 1.323104e+00 2.038772e-01
ENSG00000256087 1.209538e-01 4.021520586 1.110355e+00 2.827948e-01
ENSG00000197608 1.919544e-01 4.006239358 2.404003e+00 2.830598e-02
ENSG00000204611 -3.505352e-02 3.850670783 -4.274408e-01 6.745945e-01
ENSG00000105568 -8.331832e-02 7.439852649 -1.371001e+00 1.887690e-01
ENSG00000196214 -1.116932e-01 3.198031676 -1.383913e+00 1.848543e-01
ENSG00000198464 5.042845e-02 3.424444091 5.051079e-01 6.201750e-01
ENSG00000167554 9.467719e-02 2.135684048 7.410519e-01 4.690971e-01
ENSG00000221923 -1.041664e-01 2.348356875 -8.615520e-01 4.013042e-01
ENSG00000167555 5.609910e-02 2.377642494 4.735539e-01 6.420344e-01
ENSG00000167766 1.210421e-01 3.897495780 1.439299e+00 1.688013e-01
ENSG00000213020 4.247709e-02 2.480214607 4.089158e-01 6.878696e-01
ENSG00000189190 1.232563e-01 1.940346670 7.900108e-01 4.407485e-01
ENSG00000198538 1.790608e-01 3.912907898 1.897435e+00 7.544440e-02
ENSG00000204604 3.169256e-02 4.547968222 2.952149e-01 7.715189e-01
ENSG00000213793 1.233096e-01 3.335171849 1.182046e+00 2.539734e-01
ENSG00000180257 1.167095e-01 3.077122991 1.334408e+00 2.002275e-01
ENSG00000242779 -5.938592e-02 4.041796100 -8.114221e-01 4.286949e-01
ENSG00000170949 3.980759e-02 4.027401106 5.982795e-01 5.577923e-01
adj.P.Val B lfsr lfdr qvalue
ENSG00000225972 0.03206567 2.34201392 0.16927420 0.11421833 0.06162590
ENSG00000225630 0.22586000 0.13698082 0.25431420 0.18535011 0.14216112
ENSG00000237973 0.67698578 -3.63015398 0.45411333 0.31053360 0.26574415
ENSG00000248527 0.05680183 2.39713189 0.07918372 0.05784497 0.02672962
ENSG00000198744 0.94108198 -5.13705661 0.65051320 0.37692390 0.33407155
ENSG00000228794 0.99056607 -5.20588505 0.68370927 0.37895767 0.33637783
ENSG00000223764 0.98276879 -5.13143705 0.67822814 0.37449227 0.33078111
ENSG00000187634 0.82848167 -4.90575717 0.58861543 0.36487526 0.31773747
ENSG00000188976 0.69256556 -3.96440070 0.35066314 0.26235234 0.22219624
ENSG00000187961 0.81069335 -4.86665475 0.57296012 0.36151420 0.31373559
ENSG00000187608 0.88489224 -5.23444412 0.62597984 0.37171858 0.32683680
ENSG00000188157 0.69889082 -4.49291533 0.48377322 0.32801840 0.28028003
ENSG00000131591 0.95623844 -5.22197810 0.65631022 0.38061910 0.33823672
ENSG00000078808 0.89239880 -5.69364185 0.59689547 0.39204200 0.34751354
ENSG00000176022 0.98871281 -5.83157508 0.68580979 0.39335337 0.34842339
ENSG00000160087 0.84632247 -5.43392751 0.53728436 0.37823493 0.33565666
ENSG00000131584 0.73407810 -4.60344894 0.45579265 0.32026601 0.27438986
ENSG00000169972 0.78448036 -4.80327650 0.51652552 0.34928228 0.30076464
ENSG00000127054 0.69889082 -4.50512675 0.36498134 0.27870600 0.23616496
ENSG00000224051 0.88757226 -5.44158993 0.60211322 0.38099764 0.33864862
ENSG00000107404 0.83001904 -5.34181938 0.55853270 0.36450002 0.31728955
ENSG00000162576 0.90181821 -5.66435952 0.61809738 0.37898069 0.33641466
ENSG00000175756 0.93678307 -5.79425181 0.63503542 0.43080715 0.36203383
ENSG00000221978 0.82349008 -5.37680511 0.58113956 0.36411464 0.31683653
ENSG00000224870 0.91565222 -5.47119667 0.62007182 0.38512577 0.34256687
ENSG00000242485 0.97830792 -5.83829240 0.68256735 0.41967924 0.35939635
ENSG00000160072 0.95717112 -5.55065256 0.65457177 0.39884509 0.35141027
ENSG00000197785 0.93527929 -5.61276727 0.63245797 0.39186593 0.34741843
ENSG00000160075 0.92842091 -5.81444812 0.62586267 0.42657841 0.36114134
ENSG00000228594 0.93719317 -5.32116934 0.64616878 0.37879613 0.33620796
ENSG00000197530 0.69889082 -4.52126991 0.48645479 0.32930524 0.28154268
ENSG00000189409 0.96537609 -5.65570157 0.66241063 0.38467919 0.34219008
ENSG00000248333 0.56465058 -1.72627218 0.30669558 0.22598080 0.18918798
ENSG00000189339 0.75969524 -4.92806343 0.44811508 0.32811018 0.28038875
ENSG00000008130 0.90021819 -5.64735048 0.59586619 0.40995419 0.35637996
ENSG00000078369 0.95717112 -5.84271389 0.66811657 0.45783692 0.36569145
ENSG00000162585 0.83001904 -5.24701686 0.56682477 0.36552768 0.31866498
ENSG00000157933 0.87189376 -5.64055901 0.56920527 0.39773121 0.35084020
ENSG00000157916 0.93099810 -5.82233037 0.62906013 0.43424847 0.36270446
ENSG00000157911 0.96537609 -5.50870452 0.66203030 0.38569957 0.34299844
ENSG00000157881 0.96537609 -5.56767230 0.66376391 0.40709318 0.35521711
ENSG00000157870 0.77619856 -4.70746726 0.55067207 0.35461947 0.30583190
ENSG00000158109 0.73212605 -4.67055462 0.38039192 0.28952699 0.24595726
ENSG00000116213 0.86866914 -5.29391607 0.57699119 0.38071322 0.33835589
ENSG00000227372 0.96686686 -5.54605376 0.66459773 0.40387336 0.35383902
ENSG00000130764 0.84968600 -5.45344516 0.55085459 0.37706069 0.33422000
ENSG00000116198 0.84632247 -5.32760104 0.54962756 0.37312181 0.32881250
ENSG00000198912 0.97933520 -5.67855372 0.68208375 0.41103788 0.35685039
ENSG00000131697 0.97183826 -5.38541497 0.66832870 0.39534337 0.34960523
ENSG00000116251 0.72997233 -4.65189122 0.41306033 0.30287901 0.25824169
ENSG00000116237 0.97367105 -5.92485858 0.68374219 0.44453802 0.36440042
ENSG00000158292 0.91912614 -5.19494955 0.63509852 0.37756457 0.33487661
ENSG00000097021 0.96537609 -5.73393087 0.66540018 0.37783105 0.33519663
ENSG00000215788 0.97534428 -5.17054862 0.67372762 0.37279599 0.32825033
ENSG00000171680 0.95473561 -5.69442613 0.65660793 0.37862355 0.33601508
ENSG00000162408 0.69889082 -4.22768003 0.47025283 0.32258790 0.27606971
ENSG00000204859 0.93719317 -5.33605853 0.64278361 0.38219547 0.33981308
ENSG00000162413 0.74036206 -4.74038734 0.40104137 0.30038931 0.25636078
ENSG00000116273 0.90719414 -5.60697698 0.61012038 0.38978855 0.34610863
ENSG00000041988 0.83001904 -4.98931835 0.56376317 0.36532136 0.31841086
ENSG00000007923 0.69889082 -4.23631988 0.42084033 0.30079490 0.25678354
ENSG00000171735 0.98171416 -5.56891237 0.67701754 0.38276703 0.34038417
ENSG00000049245 0.72011795 -4.63158515 0.35381344 0.27662924 0.23491538
ENSG00000116288 0.78137975 -5.15031118 0.45307199 0.33879885 0.29029328
ENSG00000116285 0.97534428 -5.89704282 0.67286877 0.37517510 0.33173272
ENSG00000142599 0.06115857 2.13299373 0.09013278 0.06893422 0.03376372
ENSG00000074800 0.98290632 -5.87022072 0.69070817 0.42243817 0.36008862
ENSG00000142583 0.94585030 -5.09995681 0.66178601 0.37155792 0.32658438
ENSG00000180758 0.76530038 -4.55871938 0.52857867 0.34901972 0.30032730
ENSG00000228526 0.90651177 -5.22636355 0.64453852 0.37104536 0.32575537
ENSG00000049239 0.93246524 -5.77013890 0.63312836 0.38728507 0.34424890
ENSG00000171621 0.87134705 -5.63417123 0.58595005 0.38015288 0.33760539
ENSG00000171612 0.83124533 -4.92756988 0.58930821 0.36600538 0.31921600
ENSG00000188807 0.99221659 -5.39405399 0.68872651 0.38902994 0.34557739
ENSG00000171608 0.90699375 -5.33368045 0.62605074 0.37731483 0.33456922
ENSG00000171603 0.92493605 -5.80481052 0.62034137 0.40634949 0.35486978
ENSG00000178585 0.86996897 -5.32602896 0.58696046 0.37927739 0.33670091
ENSG00000162441 0.74383407 -4.62601612 0.47901736 0.33180894 0.28358061
ENSG00000173614 0.96537609 -5.50788485 0.66231551 0.38613191 0.34336385
ENSG00000130939 0.83001904 -5.40313727 0.52608120 0.36837766 0.32204040
ENSG00000054523 0.86440170 -5.59020538 0.55186576 0.39685569 0.35039764
ENSG00000142657 0.99029781 -5.94188985 0.70749516 0.43843702 0.36357064
ENSG00000160049 0.96686686 -5.81501673 0.67575863 0.44257571 0.36413639
ENSG00000142655 0.81069335 -5.00130500 0.52956689 0.35832345 0.30995150
ENSG00000120948 0.98897356 -5.85919184 0.70376965 0.43686348 0.36323225
ENSG00000116649 0.71043695 -4.60780900 0.44698427 0.31552153 0.27032041
ENSG00000171824 0.79438826 -5.10429118 0.48394171 0.34739504 0.29854620
ENSG00000198793 0.73635397 -4.70118158 0.41317056 0.30413484 0.25930552
ENSG00000171819 0.98214869 -5.73029663 0.67792663 0.37408932 0.33025150
ENSG00000120942 0.88899448 -5.47005133 0.59359788 0.39149007 0.34725780
ENSG00000116661 0.96537609 -5.18014138 0.66590123 0.37581002 0.33257653
ENSG00000132879 0.92962775 -5.20917782 0.63411664 0.38378318 0.34134845
ENSG00000116670 0.97830792 -5.51537735 0.67512351 0.39347738 0.34851736
ENSG00000177674 0.97191985 -5.70951037 0.67047503 0.40758635 0.35537660
ENSG00000177000 0.96804025 -5.62316440 0.66555048 0.40308271 0.35347146
ENSG00000011021 0.88101100 -5.46899058 0.58651353 0.38522863 0.34263053
ENSG00000116685 0.94632635 -5.76123093 0.64320996 0.40671652 0.35502282
ENSG00000083444 0.99857777 -5.85721824 0.70549747 0.41234918 0.35734661
ENSG00000116688 0.91918749 -5.79093253 0.61633568 0.41979523 0.35944712
ENSG00000116691 0.76530038 -4.69476708 0.48373852 0.33902437 0.29056200
ENSG00000048707 0.87423811 -5.60519516 0.57258438 0.39464672 0.34921400
ENSG00000162496 0.99221659 -5.37227850 0.68516963 0.37865527 0.33605225
ENSG00000162494 0.89675590 -4.96362034 0.63814946 0.37139855 0.32632999
ENSG00000162493 0.84703107 -5.43730576 0.55225684 0.37433605 0.33057372
ENSG00000116731 0.97777372 -5.85791027 0.68835093 0.43901160 0.36365970
ENSG00000189337 0.67698578 -2.90989058 0.46288689 0.31120735 0.26630093
ENSG00000171729 0.98999465 -5.39452574 0.68271023 0.37592548 0.33276405
ENSG00000142634 0.80744860 -4.99340359 0.56965153 0.36055667 0.31260139
ENSG00000132906 0.96235982 -5.47546140 0.65886243 0.39313996 0.34827643
ENSG00000116138 0.80569801 -5.17004778 0.49817892 0.35367099 0.30497227
ENSG00000197312 0.92976437 -5.68552571 0.62763327 0.39453610 0.34914975
ENSG00000116786 0.82094034 -5.35666830 0.49749848 0.36672572 0.32004033
ENSG00000162458 0.96967652 -5.91490392 0.66570559 0.39333993 0.34840576
ENSG00000065526 0.85823678 -5.47704904 0.54599361 0.38633097 0.34350193
ENSG00000116809 0.74036206 -4.57156070 0.48904228 0.33367881 0.28512513
ENSG00000173641 0.91500749 -5.61852915 0.63597804 0.37463258 0.33097868
ENSG00000142627 0.78464827 -5.14984980 0.53694238 0.35297400 0.30442027
ENSG00000142632 0.90875367 -5.19732099 0.63262541 0.37492036 0.33137892
ENSG00000037637 0.80141844 -5.08704850 0.49850842 0.35235753 0.30382878
ENSG00000055070 0.69735521 -4.13658896 0.30425334 0.23801280 0.20175439
ENSG00000157191 0.96755205 -5.88573883 0.68350880 0.45920886 0.36576304
ENSG00000219481 0.95276624 -5.65747733 0.64777761 0.40247768 0.35315087
ENSG00000215908 0.97925180 -5.50864143 0.67783419 0.39852762 0.35122404
ENSG00000058453 0.95326549 -5.59325173 0.65308009 0.38234953 0.33997514
ENSG00000117122 0.93807657 -5.84607991 0.63500432 0.41049352 0.35662995
ENSG00000159363 0.85823678 -5.52743070 0.57692456 0.37263269 0.32799823
ENSG00000117118 0.79927428 -5.22028846 0.47902854 0.34991762 0.30139362
ENSG00000179051 0.98060317 -5.91816875 0.68581877 0.41889274 0.35921297
ENSG00000074964 0.81915560 -4.97654465 0.54441910 0.36125257 0.31339299
ENSG00000159423 0.87535545 -5.08763192 0.61712462 0.37216371 0.32741261
ENSG00000169991 0.97486988 -5.72309861 0.67010327 0.39268160 0.34796963
ENSG00000127481 0.96537609 -5.84822498 0.66245156 0.40388809 0.35385652
ENSG00000127463 0.79632971 -5.23387780 0.47716611 0.34891746 0.30019876
ENSG00000053372 0.91858834 -5.55565310 0.61686493 0.39551404 0.34968681
ENSG00000053371 0.74036206 -4.66716195 0.44315206 0.31799061 0.27227713
ENSG00000040487 0.91871788 -5.54582006 0.62612710 0.38339633 0.34099424
ENSG00000077549 0.93719317 -5.83457187 0.64450449 0.45494851 0.36549071
ENSG00000173436 0.93719317 -5.26868660 0.64415775 0.37976203 0.33718077
ENSG00000158747 0.86440170 -5.55365707 0.57618285 0.37533471 0.33196653
ENSG00000169914 0.95717112 -5.70562680 0.65697766 0.38378985 0.34136151
ENSG00000188257 0.76978634 -4.54514492 0.63712544 0.36503637 0.31805749
ENSG00000127472 0.98194758 -5.37512100 0.67764213 0.38285786 0.34046417
ENSG00000162545 0.88899448 -5.52493693 0.61673284 0.37553271 0.33228931
ENSG00000090432 0.87939291 -5.60347071 0.57945240 0.39203600 0.34750165
ENSG00000158828 0.74721455 -4.75034939 0.51436299 0.34358539 0.29522865
ENSG00000117242 0.91871788 -5.14179109 0.63286179 0.37843710 0.33584367
ENSG00000244038 0.82232555 -5.35148185 0.51032764 0.36542748 0.31847152
ENSG00000117245 0.90537408 -5.11849404 0.63659863 0.37288753 0.32841725
ENSG00000189410 0.88899448 -5.11857933 0.62750059 0.37290934 0.32845422
ENSG00000127483 0.81831142 -5.36054335 0.50126604 0.36272643 0.31510451
ENSG00000075151 0.81576430 -5.34808463 0.49074604 0.36395622 0.31658268
ENSG00000117298 0.69735521 -4.13403752 0.34224429 0.25927282 0.21924466
ENSG00000142794 0.88749237 -5.23971472 0.59214841 0.38771677 0.34457626
ENSG00000162551 0.98929462 -5.36162592 0.68440635 0.38332262 0.34088874
ENSG00000090686 0.97830792 -5.90402608 0.69317950 0.44540387 0.36453423
ENSG00000142798 0.88349773 -5.59222629 0.61245255 0.37467125 0.33107276
ENSG00000070831 0.97052078 -5.92032591 0.67223200 0.42020036 0.35952979
ENSG00000162552 0.97052078 -5.60903931 0.67353283 0.37070622 0.32518061
ENSG00000184677 0.99326607 -5.77215404 0.69442496 0.39871230 0.35130551
ENSG00000133216 0.98089069 -5.32678701 0.67657454 0.37669135 0.33378857
ENSG00000004487 0.69889082 -4.36108132 0.32949568 0.25655723 0.21685072
ENSG00000169641 0.79020219 -5.20027902 0.46682758 0.34493710 0.29644764
ENSG00000179546 0.84632247 -4.88676468 0.61202560 0.36740314 0.32092789
ENSG00000125944 0.89239880 -5.71528374 0.59092012 0.41355818 0.35769025
ENSG00000125945 0.86581606 -5.26137660 0.57562440 0.37780489 0.33517386
ENSG00000088280 0.94512989 -5.35824377 0.64480724 0.38703039 0.34408786
ENSG00000117318 0.98925660 -5.90327700 0.69101141 0.40326043 0.35354724
ENSG00000142676 0.97759175 -5.90084877 0.68398269 0.42906216 0.36173041
ENSG00000011007 0.86370138 -5.56147039 0.54621227 0.39510105 0.34943606
ENSG00000057757 0.99668536 -5.81876146 0.71214017 0.42763458 0.36138396
ENSG00000011009 0.67698578 -3.29823683 0.36176511 0.26379636 0.22347796
ENSG00000117308 0.99221659 -5.63203318 0.69732920 0.40736899 0.35528208
ENSG00000117305 0.83246887 -5.28656752 0.54578449 0.36799595 0.32162830
ENSG00000179163 0.94512989 -5.46726120 0.64457294 0.38725785 0.34422414
ENSG00000189266 0.67698578 -3.41862926 0.35680037 0.26199100 0.22193491
ENSG00000188529 0.77619856 -5.10744914 0.49344883 0.34348132 0.29518823
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ENSG00000169136 0.83001904 -4.98435719 0.56489140 0.36489923 0.31778691
ENSG00000105053 0.76521922 -4.81765521 0.51842545 0.34669283 0.29791231
ENSG00000142528 0.92493605 -5.25296682 0.63335934 0.38140115 0.33903600
ENSG00000131408 0.97052078 -5.85714631 0.66738234 0.40218682 0.35297575
ENSG00000062822 0.95717112 -5.30321148 0.65793034 0.38227968 0.33990094
ENSG00000161671 0.86440170 -5.57426485 0.56258169 0.37923779 0.33667164
ENSG00000161677 0.87535545 -5.47086236 0.59013733 0.38041660 0.33789769
ENSG00000167747 0.97777372 -5.64209151 0.67480927 0.39737374 0.35064813
ENSG00000142544 0.96335224 -5.15130893 0.66885262 0.37188861 0.32703935
ENSG00000105379 0.84632247 -5.05733767 0.58826395 0.36789153 0.32152744
ENSG00000105497 0.85823678 -5.07351596 0.57423107 0.37314101 0.32885813
ENSG00000182310 0.97838568 -5.22421594 0.67473485 0.38336362 0.34092835
ENSG00000198093 0.77619856 -4.55946115 0.57317520 0.35812921 0.30966645
ENSG00000176024 0.79576594 -4.69101568 0.54881155 0.35644085 0.30782217
ENSG00000256683 0.96967652 -5.19224540 0.66634717 0.38238481 0.34002227
ENSG00000197619 0.67698578 -2.94129798 0.49452626 0.32350733 0.27667729
ENSG00000142556 0.77619856 -4.73007028 0.53380715 0.35148876 0.30311094
ENSG00000256087 0.81860856 -5.06133593 0.57196804 0.36260594 0.31502521
ENSG00000197608 0.67698578 -3.37503084 0.39110628 0.28002380 0.23707228
ENSG00000204611 0.94493176 -5.47921529 0.64156092 0.39348315 0.34852322
ENSG00000105568 0.76530038 -5.07043075 0.46660957 0.33508616 0.28657779
ENSG00000196214 0.76512406 -4.64396837 0.50436593 0.34337465 0.29514779
ENSG00000198464 0.93209148 -5.34080305 0.63568834 0.38347996 0.34106001
ENSG00000167554 0.88443557 -4.95457982 0.62044801 0.37280318 0.32828750
ENSG00000221923 0.86440170 -4.96365002 0.60559341 0.37041073 0.32470911
ENSG00000167555 0.93678307 -5.29907559 0.64398039 0.37943766 0.33689059
ENSG00000167766 0.75267407 -4.69487989 0.50309464 0.34128944 0.29321480
ENSG00000213020 0.94592528 -5.15793983 0.64799903 0.38430718 0.34179703
ENSG00000189190 0.87189376 -4.90974236 0.62642299 0.37014305 0.32431461
ENSG00000198538 0.69889082 -4.11187586 0.47275803 0.32237719 0.27577859
ENSG00000204604 0.96353871 -5.66262800 0.66135486 0.38503886 0.34249679
ENSG00000213793 0.80572737 -4.87206166 0.56003664 0.35944170 0.31120307
ENSG00000180257 0.77368432 -4.67435824 0.52164597 0.34878977 0.29994020
ENSG00000242779 0.86866914 -5.28892623 0.57944208 0.38083070 0.33850251
ENSG00000170949 0.91565222 -5.44170046 0.61095110 0.39766519 0.35080529
svalue
ENSG00000225972 0.10554713
ENSG00000225630 0.20075277
ENSG00000237973 0.37513704
ENSG00000248527 0.03661649
ENSG00000198744 0.53102010
ENSG00000228794 0.55694548
ENSG00000223764 0.55276926
ENSG00000187634 0.48251510
ENSG00000188976 0.29144004
ENSG00000187961 0.46988413
ENSG00000187608 0.51185988
ENSG00000188157 0.39835447
ENSG00000131591 0.53556710
ENSG00000078808 0.48949126
ENSG00000176022 0.55868543
ENSG00000160087 0.43968510
ENSG00000131584 0.37628647
ENSG00000169972 0.42296905
ENSG00000127054 0.30387583
ENSG00000224051 0.49329755
ENSG00000107404 0.45766729
ENSG00000162576 0.50579640
ENSG00000175756 0.51903955
ENSG00000221978 0.47681313
ENSG00000224870 0.50724464
ENSG00000242485 0.55612824
ENSG00000160072 0.53398351
ENSG00000197785 0.51664297
ENSG00000160075 0.51175484
ENSG00000228594 0.52733371
ENSG00000197530 0.40022461
ENSG00000189409 0.54023245
ENSG00000248333 0.25595522
ENSG00000189339 0.37030454
ENSG00000008130 0.48860178
ENSG00000078369 0.54482287
ENSG00000162585 0.46442613
ENSG00000157933 0.46662536
ENSG00000157916 0.51415464
ENSG00000157911 0.53981900
ENSG00000157881 0.54129038
ENSG00000157870 0.45097566
ENSG00000158109 0.31565327
ENSG00000116213 0.47350766
ENSG00000227372 0.54219456
ENSG00000130764 0.45113842
ENSG00000116198 0.45004839
ENSG00000198912 0.55565782
ENSG00000131697 0.54500902
ENSG00000116251 0.34427973
ENSG00000116237 0.55695884
ENSG00000158292 0.51910638
ENSG00000097021 0.54271701
ENSG00000215788 0.54893203
ENSG00000171680 0.53577878
ENSG00000162408 0.38753343
ENSG00000204859 0.52472802
ENSG00000162413 0.33378308
ENSG00000116273 0.49944356
ENSG00000041988 0.46199350
ENSG00000007923 0.35015969
ENSG00000171735 0.55169901
ENSG00000049245 0.29374974
ENSG00000116288 0.37453418
ENSG00000116285 0.54838424
ENSG00000142599 0.04553587
ENSG00000074800 0.56200726
ENSG00000142583 0.53965617
ENSG00000180758 0.43289546
ENSG00000228526 0.52602077
ENSG00000049239 0.51718699
ENSG00000171621 0.48035060
ENSG00000171612 0.48315106
ENSG00000188807 0.56059508
ENSG00000171608 0.51194733
ENSG00000171603 0.50733478
ENSG00000178585 0.48095401
ENSG00000162441 0.39482154
ENSG00000173614 0.54011448
ENSG00000130939 0.43103332
ENSG00000054523 0.45197603
ENSG00000142657 0.56788891
ENSG00000160049 0.55062072
ENSG00000142655 0.43358292
ENSG00000120948 0.56707020
ENSG00000116649 0.36924653
ENSG00000171824 0.39843630
ENSG00000198793 0.34441200
ENSG00000171819 0.55260519
ENSG00000120942 0.48649233
ENSG00000116661 0.54303588
ENSG00000132879 0.51815080
ENSG00000116670 0.55010634
ENSG00000177674 0.54663352
ENSG00000177000 0.54278951
ENSG00000011021 0.48073853
ENSG00000116685 0.52493866
ENSG00000083444 0.56747107
ENSG00000116688 0.50416668
ENSG00000116691 0.39827259
ENSG00000048707 0.46953407
ENSG00000162496 0.55831482
ENSG00000162494 0.52147803
ENSG00000162493 0.45243289
ENSG00000116731 0.56034544
ENSG00000189337 0.38159504
ENSG00000171729 0.55623557
ENSG00000142634 0.46705517
ENSG00000132906 0.53733146
ENSG00000116138 0.40914373
ENSG00000197312 0.51302788
ENSG00000116786 0.40865335
ENSG00000162458 0.54290547
ENSG00000065526 0.44716929
ENSG00000116809 0.40230548
ENSG00000173641 0.51980658
ENSG00000142627 0.43944651
ENSG00000142632 0.51677921
ENSG00000037637 0.40940717
ENSG00000055070 0.25340412
ENSG00000157191 0.55678498
ENSG00000219481 0.52866769
ENSG00000215908 0.55249568
ENSG00000058453 0.53284657
ENSG00000117122 0.51900612
ENSG00000159363 0.47343990
ENSG00000117118 0.39486356
ENSG00000179051 0.55871188
ENSG00000074964 0.44570427
ENSG00000159423 0.50470022
ENSG00000169991 0.54626432
ENSG00000127481 0.54024718
ENSG00000127463 0.39364124
ENSG00000053372 0.50449803
ENSG00000053371 0.36672433
ENSG00000040487 0.51201724
ENSG00000077549 0.52594029
ENSG00000173436 0.52574692
ENSG00000158747 0.47282834
ENSG00000169914 0.53602055
ENSG00000188257 0.52066951
ENSG00000127472 0.55231757
ENSG00000162545 0.50436920
ENSG00000090432 0.47539364
ENSG00000158828 0.42107820
ENSG00000117242 0.51701723
ENSG00000244038 0.41809826
ENSG00000117245 0.52020526
ENSG00000189410 0.51299310
ENSG00000127483 0.41120484
ENSG00000075151 0.40383976
ENSG00000117298 0.28329604
ENSG00000142794 0.48545706
ENSG00000162551 0.55767834
ENSG00000090686 0.56310233
ENSG00000142798 0.50112284
ENSG00000070831 0.54789183
ENSG00000162552 0.54879171
ENSG00000184677 0.56356050
ENSG00000133216 0.55134046
ENSG00000004487 0.27227846
ENSG00000169641 0.38465038
ENSG00000179546 0.50085982
ENSG00000125944 0.48447657
ENSG00000125945 0.47216753
ENSG00000088280 0.52627788
ENSG00000117318 0.56224192
ENSG00000142676 0.55725255
ENSG00000011007 0.44733698
ENSG00000057757 0.56861387
ENSG00000011009 0.30122357
ENSG00000117308 0.56488531
ENSG00000117305 0.44691714
ENSG00000179163 0.52605295
ENSG00000189266 0.29681282
ENSG00000188529 0.40555451
ENSG00000001460 0.51342732
ENSG00000001461 0.50566914
ENSG00000117602 0.54664770
ENSG00000133226 0.43764466
ENSG00000169504 0.43920752
ENSG00000117614 0.53098899
ENSG00000117616 0.45629832
ENSG00000183726 0.54134895
ENSG00000204178 0.36409311
ENSG00000157978 0.48910796
ENSG00000117643 0.44060480
ENSG00000162430 0.26318539
ENSG00000117640 0.56545106
ENSG00000182749 0.55770491
ENSG00000117632 0.48711075
ENSG00000158006 0.49258618
ENSG00000158008 0.41728215
ENSG00000158022 0.47698952
ENSG00000130695 0.52344453
ENSG00000142669 0.34368084
ENSG00000158062 0.48609960
ENSG00000117682 0.36263787
ENSG00000198830 0.51890579
ENSG00000117713 0.39137495
ENSG00000060642 0.54897411
ENSG00000204160 0.52563409
ENSG00000142751 0.46559143
ENSG00000198746 0.56432067
ENSG00000090273 0.37737667
ENSG00000253368 0.50866369
ENSG00000158246 0.50484703
ENSG00000090020 0.50762304
ENSG00000142784 0.44081170
ENSG00000186501 0.35078200
ENSG00000142733 0.54936628
ENSG00000158195 0.28475570
ENSG00000126705 0.39941477
ENSG00000126709 0.55464533
ENSG00000009780 0.55793057
ENSG00000117758 0.41689039
ENSG00000117751 0.51373936
ENSG00000117748 0.50939359
ENSG00000158156 0.55502378
ENSG00000158161 0.56663036
ENSG00000126698 0.46537382
ENSG00000130770 0.39637075
ENSG00000130766 0.56158984
ENSG00000130772 0.51638721
ENSG00000204138 0.45173351
ENSG00000180198 0.55555004
ENSG00000242125 0.55335614
ENSG00000180098 0.53199742
ENSG00000197989 0.55330164
ENSG00000120656 0.47303250
ENSG00000162419 0.36353622
ENSG00000198492 0.40899294
ENSG00000159023 0.41465947
ENSG00000253304 0.50411144
ENSG00000116350 0.45064910
ENSG00000116353 0.48439283
ENSG00000060656 0.46079500
ENSG00000162512 0.51662593
ENSG00000134644 0.14932735
ENSG00000060688 0.41298116
ENSG00000121766 0.50925148
ENSG00000121769 0.54535226
ENSG00000168528 0.50614160
ENSG00000162517 0.56737718
ENSG00000084636 0.50992499
ENSG00000121753 0.55666448
ENSG00000134668 0.49987938
ENSG00000184007 0.55701231
ENSG00000121774 0.56927132
ENSG00000121775 0.52624577
ENSG00000025800 0.56151151
ENSG00000084652 0.49199111
ENSG00000160050 0.44021990
ENSG00000160055 0.44914261
ENSG00000084623 0.55707909
ENSG00000220785 0.49874211
ENSG00000116478 0.22370405
ENSG00000175130 0.44433413
ENSG00000160058 0.42422088
ENSG00000160062 0.54029140
ENSG00000176261 0.40970820
ENSG00000162521 0.54650583
ENSG00000162520 0.52790690
ENSG00000162522 0.42180404
ENSG00000134684 0.41042160
ENSG00000116497 0.34321109
ENSG00000160097 0.49345513
ENSG00000116514 0.50550525
ENSG00000004455 0.55910830
ENSG00000142920 0.46454807
ENSG00000116525 0.54652002
ENSG00000160094 0.54202009
ENSG00000134686 0.41094425
ENSG00000121903 0.48811382
ENSG00000121904 0.53497569
ENSG00000163866 0.41985919
ENSG00000163867 0.35707325
ENSG00000197056 0.36178528
ENSG00000116560 0.41678342
ENSG00000146463 0.38589261
ENSG00000142687 0.51346199
ENSG00000020129 0.32870810
ENSG00000126067 0.42704037
ENSG00000142686 0.51115859
ENSG00000134698 0.47097317
ENSG00000092847 0.51546367
ENSG00000126070 0.25844089
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ENSG00000171812 0.55797036
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ENSG00000116871 0.45102994
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ENSG00000142694 0.52651855
ENSG00000196182 0.47512577
ENSG00000181817 0.56538518
ENSG00000116898 0.40337020
ENSG00000163874 0.42225113
ENSG00000163875 0.37855601
ENSG00000163877 0.54886188
ENSG00000134697 0.20331119
ENSG00000116922 0.34857463
ENSG00000196449 0.52566633
ENSG00000197982 0.50379852
ENSG00000188786 0.32055574
ENSG00000204084 0.48260004
ENSG00000183431 0.54749675
ENSG00000183386 0.55341061
ENSG00000183520 0.51717002
ENSG00000116954 0.42927802
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ENSG00000174574 0.50617789
ENSG00000168653 0.25864185
ENSG00000127603 0.45818065
ENSG00000090621 0.52706236
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ENSG00000043514 0.53293903
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ENSG00000131236 0.39953669
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ENSG00000084070 0.53943397
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ENSG00000171793 0.44530613
ENSG00000010803 0.47775963
ENSG00000127124 0.44559075
ENSG00000198815 0.50198338
ENSG00000127125 0.32927059
ENSG00000171960 0.55256414
ENSG00000065978 0.49799865
ENSG00000117385 0.46496173
ENSG00000164010 0.31181708
ENSG00000164011 0.42834403
ENSG00000117394 0.48336228
ENSG00000117395 0.55496972
ENSG00000066322 0.53002092
ENSG00000159479 0.23290848
ENSG00000198198 0.50488370
ENSG00000142949 0.28179497
ENSG00000066135 0.13333187
ENSG00000117408 0.24800832
ENSG00000132768 0.52958177
ENSG00000117410 0.49959515
ENSG00000117411 0.55391352
ENSG00000196517 0.52800222
ENSG00000178028 0.42324100
ENSG00000117419 0.56371770
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ENSG00000126088 0.56839317
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ENSG00000117448 0.50921592
ENSG00000132780 0.35772094
ENSG00000159592 0.29221562
ENSG00000159596 0.54490883
ENSG00000197429 0.53907791
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ENSG00000117461 0.19873009
ENSG00000085998 0.56741738
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ENSG00000173660 0.47101933
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ENSG00000142961 0.45627235
ENSG00000123472 0.39599482
ENSG00000159658 0.42462393
ENSG00000123473 0.53376897
ENSG00000162368 0.20744681
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ENSG00000162378 0.51273223
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ENSG00000162384 0.47731974
ENSG00000162385 0.42197626
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ENSG00000058799 0.55967513
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ENSG00000169174 0.51340998
ENSG00000162402 0.36503410
ENSG00000162407 0.52060330
ENSG00000162600 0.46074475
ENSG00000162601 0.49986044
ENSG00000177606 0.36359208
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ENSG00000116641 0.56320696
ENSG00000125703 0.47559411
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ENSG00000142892 0.36275072
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ENSG00000122420 0.39279223
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ENSG00000153936 0.39359892
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ENSG00000171488 0.55121616
ENSG00000171492 0.39678706
ENSG00000162664 0.39674552
ENSG00000122482 0.52854111
ENSG00000069702 0.54653422
ENSG00000174842 0.46631359
ENSG00000122484 0.23027005
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ENSG00000122406 0.47264658
ENSG00000154511 0.46327679
ENSG00000143033 0.52252877
ENSG00000117500 0.42953455
ENSG00000117505 0.31221585
ENSG00000137942 0.54727073
ENSG00000137936 0.54040923
ENSG00000067334 0.21068798
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ENSG00000117528 0.53588458
ENSG00000117525 0.56558296
ENSG00000117519 0.50119793
ENSG00000235501 0.54620744
ENSG00000172339 0.48204669
ENSG00000117569 0.50035172
ENSG00000162627 0.34978279
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ENSG00000162688 0.53372296
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ENSG00000122435 0.33340380
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ENSG00000137996 0.53653393
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ENSG00000117543 0.40532182
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ENSG00000162636 0.53612629
ENSG00000134186 0.44947250
ENSG00000116266 0.53005221
ENSG00000121940 0.53017738
ENSG00000085433 0.52170837
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[ reached 'max' / getOption("max.print") -- omitted 448 rows ]
[[8]]
logFC AveExpr t P.Value
ENSG00000225972 6.176263e-02 3.439734306 2.460221e-01 0.808784223
ENSG00000225630 1.872325e-01 5.259763521 1.759984e+00 0.097455791
ENSG00000237973 5.495285e-02 5.765427761 4.462037e-01 0.661412205
ENSG00000248527 2.219403e-01 7.291375670 1.934800e+00 0.070843374
ENSG00000198744 1.230114e-01 1.692163426 5.731332e-01 0.574498809
ENSG00000228794 -1.870802e-01 2.429990051 -1.329676e+00 0.202218143
ENSG00000223764 1.625400e-01 1.604113326 9.207808e-01 0.370800793
ENSG00000187634 1.887467e-01 2.774428967 1.471774e+00 0.160421536
ENSG00000188976 8.381312e-03 5.602324306 1.211967e-01 0.905039846
ENSG00000187961 -7.831098e-02 3.205023751 -6.338478e-01 0.535111410
ENSG00000187608 -2.904265e-01 3.515541854 -2.106990e+00 0.051202556
ENSG00000188157 7.494317e-02 8.438003633 7.163728e-01 0.484057157
ENSG00000131591 8.687687e-02 2.698849622 7.362383e-01 0.472211894
ENSG00000078808 2.877867e-02 8.404430472 4.099929e-01 0.687232519
ENSG00000176022 2.915388e-02 5.315643527 3.131972e-01 0.758164807
ENSG00000160087 3.440625e-02 5.574117382 5.931475e-01 0.561349870
ENSG00000131584 9.982131e-02 5.338644789 1.270116e+00 0.222151524
ENSG00000169972 4.576278e-02 3.649507997 5.415361e-01 0.595577525
ENSG00000127054 6.530279e-02 5.813712471 1.219928e+00 0.240118147
ENSG00000224051 1.070971e-01 4.433660580 1.255486e+00 0.227276990
ENSG00000107404 -5.319495e-04 5.541314411 -5.516923e-03 0.995666158
ENSG00000162576 4.302085e-02 5.643016003 4.104292e-01 0.686918938
ENSG00000175756 1.819987e-03 5.949578662 3.497059e-02 0.972534501
ENSG00000221978 -2.486562e-03 6.117640537 -2.055987e-02 0.983850231
ENSG00000224870 1.271493e-01 4.193043833 1.471516e+00 0.160490473
ENSG00000242485 8.835333e-02 5.474356677 1.456441e+00 0.164558372
ENSG00000160072 -6.491056e-02 4.020742816 -8.267821e-01 0.420484903
ENSG00000197785 -7.570493e-03 4.667370310 -8.825738e-02 0.930764152
ENSG00000160075 -3.282892e-02 6.698987873 -6.370295e-01 0.533089103
ENSG00000228594 -6.518548e-02 3.231082811 -5.384188e-01 0.597677908
ENSG00000197530 7.546450e-02 6.011139637 7.189284e-01 0.482523485
ENSG00000189409 -3.332827e-02 4.590321361 -3.035718e-01 0.765354682
ENSG00000248333 1.486426e-01 3.904469734 1.628646e+00 0.122858780
ENSG00000189339 6.419810e-02 5.329578573 1.103204e+00 0.286208516
ENSG00000008130 -2.761397e-04 5.472226379 -5.013078e-03 0.996061952
ENSG00000078369 9.286559e-03 8.804321589 2.098025e-01 0.836462300
ENSG00000162585 3.112726e-02 4.754428869 3.112766e-01 0.759597670
ENSG00000157933 7.476877e-02 6.562145698 1.431775e+00 0.171397761
ENSG00000157916 3.647914e-02 7.002652621 7.727153e-01 0.450922984
ENSG00000157911 8.804946e-02 3.826112744 7.994561e-01 0.435700634
ENSG00000157881 -5.312394e-02 4.048444809 -7.324617e-01 0.474450237
ENSG00000157870 -2.983027e-02 3.237377913 -2.582520e-01 0.799495355
ENSG00000158109 9.136394e-02 6.732297019 1.826454e+00 0.086443939
ENSG00000116213 1.171513e-01 4.048572327 1.681432e+00 0.112034634
ENSG00000227372 -3.824039e-02 3.931432132 -5.133947e-01 0.614671000
ENSG00000130764 -4.415521e-02 5.498222486 -6.757553e-01 0.508818126
ENSG00000116198 -4.474903e-02 4.799410870 -6.235362e-01 0.541694477
ENSG00000198912 -8.665235e-03 4.517142545 -1.232367e-01 0.903449983
ENSG00000131697 1.226924e-02 3.217207294 1.391234e-01 0.891083696
ENSG00000116251 -4.650169e-02 6.491595851 -7.070120e-01 0.489699462
ENSG00000116237 -4.503863e-03 7.144503644 -8.961698e-02 0.929700583
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ENSG00000104805 3.605080e-02 7.906149214 4.624058e-01 0.649998221
ENSG00000087088 -3.399446e-02 6.436261320 -3.572338e-01 0.725571434
ENSG00000087086 -7.327367e-02 12.058941043 -1.055556e+00 0.306804487
ENSG00000104812 1.070718e-02 5.554919935 1.930360e-01 0.849352821
ENSG00000183207 -3.235921e-02 5.649854617 -4.329406e-01 0.670820634
ENSG00000104852 3.912160e-02 6.570625396 4.254449e-01 0.676163022
ENSG00000104863 -1.126178e-01 2.860985462 -9.416400e-01 0.360337695
ENSG00000177380 -1.550379e-01 2.904772987 -1.189213e+00 0.251655727
ENSG00000130529 4.592777e-02 3.954710118 3.653477e-01 0.719622775
ENSG00000074219 -1.810238e-02 4.784127661 -2.328248e-01 0.818841059
ENSG00000104872 -9.335067e-03 5.215611657 -1.268477e-01 0.900636760
ENSG00000161618 5.448683e-02 4.073193668 4.818770e-01 0.636399503
ENSG00000142541 1.638497e-02 8.798495572 2.815767e-01 0.781866817
ENSG00000142534 3.844859e-02 9.214561396 5.423613e-01 0.595022134
ENSG00000104870 7.200377e-02 5.305007871 9.149739e-01 0.373750003
ENSG00000142552 8.699436e-02 6.302870351 1.156840e+00 0.264269187
ENSG00000142546 8.723633e-02 5.367518939 1.435907e+00 0.170235986
ENSG00000126464 6.543727e-02 4.885551572 7.267405e-01 0.477853260
ENSG00000126458 -1.054718e-02 6.339176461 -1.957309e-01 0.847277786
ENSG00000126461 5.592154e-02 6.133402435 5.098703e-01 0.617082925
ENSG00000126456 6.087694e-02 4.969957329 8.476125e-01 0.409118517
ENSG00000126453 -1.351659e-02 2.842914353 -1.158314e-01 0.909223281
ENSG00000126457 4.226455e-02 6.324574391 6.402110e-01 0.531071172
ENSG00000196961 8.062518e-02 6.862993870 1.084253e+00 0.294274471
ENSG00000010361 1.527007e-01 2.746988061 1.222846e+00 0.239043821
ENSG00000104973 1.066753e-01 4.644685303 1.116998e+00 0.280441113
ENSG00000104960 4.388697e-02 5.126602512 6.260570e-01 0.540081116
ENSG00000039650 1.344034e-01 4.318555072 1.884289e+00 0.077772857
ENSG00000204673 1.147502e-02 6.615684364 1.501699e-01 0.882501902
ENSG00000104946 8.332156e-03 5.142717077 1.090468e-01 0.914517346
ENSG00000213024 -5.412198e-02 5.178261322 -9.559082e-01 0.353298685
ENSG00000169136 -1.543839e-03 3.340949893 -1.619925e-02 0.987275134
ENSG00000105053 -5.854075e-02 4.303539969 -6.084100e-01 0.551430944
ENSG00000142528 -7.708220e-02 3.054946621 -6.878305e-01 0.501381671
ENSG00000131408 -8.953949e-03 5.748430698 -1.151442e-01 0.909759350
ENSG00000062822 6.258261e-02 3.000646471 5.423575e-01 0.595024662
ENSG00000161671 6.090470e-02 6.833897974 8.884431e-01 0.387426141
ENSG00000161677 5.767221e-02 4.863914065 7.185351e-01 0.482759355
ENSG00000167747 -7.008569e-02 4.345002868 -7.792271e-01 0.447185967
ENSG00000142544 -1.479823e-01 2.311663930 -6.531281e-01 0.522922013
ENSG00000105379 -5.705691e-02 3.407808788 -4.984034e-01 0.624961591
ENSG00000105497 -1.214110e-02 3.196139230 -1.424049e-01 0.888532824
ENSG00000182310 2.313231e-02 2.485832085 2.041609e-01 0.840794543
ENSG00000198093 1.398718e-01 2.053797321 9.871583e-01 0.338216577
ENSG00000176024 -1.111898e-01 2.592669391 -1.084779e+00 0.294048289
ENSG00000256683 -6.273315e-02 2.455207926 -5.195982e-01 0.610436635
ENSG00000197619 -2.298483e-01 2.482955439 -1.336106e+00 0.200153990
ENSG00000142556 6.786424e-02 3.382865635 6.848629e-01 0.503203353
ENSG00000256087 -1.596735e-01 4.021520586 -1.409120e+00 0.177882929
ENSG00000197608 -4.053469e-02 4.006239358 -4.194408e-01 0.680455341
ENSG00000204611 -2.783246e-02 3.850670783 -3.195359e-01 0.753442409
ENSG00000105568 1.005735e-02 7.439852649 1.502948e-01 0.882404967
ENSG00000196214 3.913874e-02 3.198031676 4.447906e-01 0.662411926
ENSG00000198464 1.415984e-01 3.424444091 1.522576e+00 0.147326319
ENSG00000167554 1.141215e-01 2.135684048 8.528381e-01 0.406298786
ENSG00000221923 2.812207e-02 2.348356875 1.950161e-01 0.847828086
ENSG00000167555 -2.686262e-02 2.377642494 -1.795900e-01 0.859722547
ENSG00000167766 2.701092e-02 3.897495780 3.015553e-01 0.766863776
ENSG00000213020 -2.353378e-02 2.480214607 -2.142747e-01 0.833031948
ENSG00000189190 -2.969432e-01 1.940346670 -1.888472e+00 0.077176927
ENSG00000198538 -5.845894e-02 3.912907898 -5.424518e-01 0.594961245
ENSG00000204604 9.122106e-02 4.547968222 8.194336e-01 0.424542802
ENSG00000213793 -1.863942e-01 3.335171849 -1.752916e+00 0.098696176
ENSG00000180257 -1.795365e-01 3.077122991 -2.112929e+00 0.050623695
ENSG00000242779 -3.323222e-02 4.041796100 -4.625081e-01 0.649926434
ENSG00000170949 4.436089e-02 4.027401106 6.614449e-01 0.517712572
adj.P.Val B lfsr lfdr qvalue svalue
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ENSG00000188157 0.9998519 -5.042677 1 1 1 1
ENSG00000131591 0.9998519 -4.730064 1 1 1 1
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ENSG00000127054 0.9998519 -4.690739 1 1 1 1
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ENSG00000107404 0.9998519 -5.195665 1 1 1 1
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ENSG00000049245 0.9998519 -4.997140 1 1 1 1
ENSG00000116288 0.9998519 -5.228697 1 1 1 1
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ENSG00000142599 0.9998519 -3.996220 1 1 1 1
ENSG00000074800 0.9998519 -5.205277 1 1 1 1
ENSG00000142583 0.9998519 -4.535331 1 1 1 1
ENSG00000180758 0.9998519 -4.721859 1 1 1 1
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ENSG00000049239 0.9998519 -5.058378 1 1 1 1
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ENSG00000162441 0.9998519 -3.330768 1 1 1 1
ENSG00000173614 0.9998519 -4.935707 1 1 1 1
ENSG00000130939 0.9998519 -5.192278 1 1 1 1
ENSG00000054523 0.9998519 -5.257462 1 1 1 1
ENSG00000142657 0.9998519 -5.214245 1 1 1 1
ENSG00000160049 0.9998519 -5.192228 1 1 1 1
ENSG00000142655 0.9998519 -4.972468 1 1 1 1
ENSG00000120948 0.9998519 -5.113684 1 1 1 1
ENSG00000116649 0.9998519 -5.195862 1 1 1 1
ENSG00000171824 0.9998519 -5.157541 1 1 1 1
ENSG00000198793 0.9998519 -5.045543 1 1 1 1
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ENSG00000120942 0.9998519 -4.618787 1 1 1 1
ENSG00000116661 0.9998519 -4.773355 1 1 1 1
ENSG00000132879 0.9998519 -4.742945 1 1 1 1
ENSG00000116670 0.9998519 -4.506457 1 1 1 1
ENSG00000177674 0.9998519 -4.673781 1 1 1 1
ENSG00000177000 0.9998519 -5.022591 1 1 1 1
ENSG00000011021 0.9998519 -4.744434 1 1 1 1
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ENSG00000083444 0.9998519 -5.182388 1 1 1 1
ENSG00000116688 0.9998519 -5.245393 1 1 1 1
ENSG00000116691 0.9998519 -4.643700 1 1 1 1
ENSG00000048707 0.9998519 -5.230849 1 1 1 1
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ENSG00000189337 0.9998519 -4.204716 1 1 1 1
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ENSG00000037637 0.9998519 -5.008523 1 1 1 1
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ENSG00000157191 0.9998519 -5.189166 1 1 1 1
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ENSG00000179051 0.9998519 -4.960145 1 1 1 1
ENSG00000074964 0.9998519 -3.737965 1 1 1 1
ENSG00000159423 0.9998519 -4.822624 1 1 1 1
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ENSG00000127463 0.9998519 -5.193421 1 1 1 1
ENSG00000053372 0.9998519 -4.987426 1 1 1 1
ENSG00000053371 0.9998519 -4.541223 1 1 1 1
ENSG00000040487 0.9998519 -5.048971 1 1 1 1
ENSG00000077549 0.9998519 -5.252841 1 1 1 1
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ENSG00000169914 0.9998519 -5.101077 1 1 1 1
ENSG00000188257 0.9998519 -3.683331 1 1 1 1
ENSG00000127472 0.9998519 -4.815946 1 1 1 1
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ENSG00000142676 0.9998519 -4.400098 1 1 1 1
ENSG00000011007 0.9998519 -4.520728 1 1 1 1
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ENSG00000001460 0.9998519 -4.742413 1 1 1 1
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ENSG00000182013 0.9998519 -4.633733 1 1 1 1
ENSG00000160014 0.9998519 -5.156063 1 1 1 1
ENSG00000197380 0.9998519 -4.700670 1 1 1 1
ENSG00000105287 0.9998519 -4.922434 1 1 1 1
ENSG00000090372 0.9998519 -4.416683 1 1 1 1
ENSG00000181027 0.9998519 -4.812475 1 1 1 1
ENSG00000105281 0.9998519 -4.923810 1 1 1 1
ENSG00000042753 0.9998519 -5.087989 1 1 1 1
ENSG00000160007 0.9998519 -4.780951 1 1 1 1
ENSG00000130751 0.9998519 -4.695869 1 1 1 1
ENSG00000130748 0.9998519 -4.911711 1 1 1 1
ENSG00000130749 0.9998519 -4.480831 1 1 1 1
ENSG00000142230 0.9998519 -5.147464 1 1 1 1
ENSG00000105327 0.9998519 -4.798194 1 1 1 1
ENSG00000105321 0.9998519 -5.096776 1 1 1 1
ENSG00000257704 0.9998519 -4.900522 1 1 1 1
ENSG00000134815 0.9998519 -4.749286 1 1 1 1
ENSG00000105419 0.9998519 -5.092376 1 1 1 1
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ENSG00000063169 0.9998519 -4.046505 1 1 1 1
ENSG00000024422 0.9998519 -4.691094 1 1 1 1
ENSG00000105373 0.9998519 -4.665181 1 1 1 1
ENSG00000178980 0.9998519 -4.941854 1 1 1 1
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ENSG00000105486 0.9998519 -4.873597 1 1 1 1
ENSG00000185453 0.9998519 -4.729644 1 1 1 1
ENSG00000105483 0.9998519 -4.887026 1 1 1 1
ENSG00000142227 0.9998519 -5.219790 1 1 1 1
ENSG00000105438 0.9998519 -5.005457 1 1 1 1
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ENSG00000087076 0.9998519 -4.297180 1 1 1 1
ENSG00000087074 0.9998519 -5.258438 1 1 1 1
ENSG00000104805 0.9998519 -5.163111 1 1 1 1
ENSG00000087088 0.9998519 -5.207813 1 1 1 1
ENSG00000087086 0.9998519 -4.729174 1 1 1 1
ENSG00000104812 0.9998519 -5.191429 1 1 1 1
ENSG00000183207 0.9998519 -5.136724 1 1 1 1
ENSG00000104852 0.9998519 -5.190509 1 1 1 1
ENSG00000104863 0.9998519 -4.666140 1 1 1 1
ENSG00000177380 0.9998519 -4.571980 1 1 1 1
ENSG00000130529 0.9998519 -4.963769 1 1 1 1
ENSG00000074219 0.9998519 -5.100305 1 1 1 1
ENSG00000104872 0.9998519 -5.162256 1 1 1 1
ENSG00000161618 0.9998519 -4.967209 1 1 1 1
ENSG00000142541 0.9998519 -5.186586 1 1 1 1
ENSG00000142534 0.9998519 -5.092190 1 1 1 1
ENSG00000104870 0.9998519 -4.893572 1 1 1 1
ENSG00000142552 0.9998519 -4.730149 1 1 1 1
ENSG00000142546 0.9998519 -4.482105 1 1 1 1
ENSG00000126464 0.9998519 -4.950547 1 1 1 1
ENSG00000126458 0.9998519 -5.237290 1 1 1 1
ENSG00000126461 0.9998519 -5.147253 1 1 1 1
ENSG00000126456 0.9998519 -4.889431 1 1 1 1
ENSG00000126453 0.9998519 -4.847462 1 1 1 1
ENSG00000126457 0.9998519 -5.094932 1 1 1 1
ENSG00000196961 0.9998519 -4.822638 1 1 1 1
ENSG00000010361 0.9998519 -4.554958 1 1 1 1
ENSG00000104973 0.9998519 -4.703102 1 1 1 1
ENSG00000104960 0.9998519 -5.024690 1 1 1 1
ENSG00000039650 0.9998519 -4.068156 1 1 1 1
ENSG00000204673 0.9998519 -5.250571 1 1 1 1
ENSG00000104946 0.9998519 -5.164339 1 1 1 1
ENSG00000213024 0.9998519 -4.848696 1 1 1 1
ENSG00000169136 0.9998519 -4.929851 1 1 1 1
ENSG00000105053 0.9998519 -4.934153 1 1 1 1
ENSG00000142528 0.9998519 -4.763453 1 1 1 1
ENSG00000131408 0.9998519 -5.214397 1 1 1 1
ENSG00000062822 0.9998519 -4.812460 1 1 1 1
ENSG00000161671 0.9998519 -4.961875 1 1 1 1
ENSG00000161677 0.9998519 -4.950487 1 1 1 1
ENSG00000167747 0.9998519 -4.856782 1 1 1 1
ENSG00000142544 0.9998519 -4.708125 1 1 1 1
ENSG00000105379 0.9998519 -4.866539 1 1 1 1
ENSG00000105497 0.9998519 -4.902245 1 1 1 1
ENSG00000182310 0.9998519 -4.821849 1 1 1 1
ENSG00000198093 0.9998519 -4.620336 1 1 1 1
ENSG00000176024 0.9998519 -4.606191 1 1 1 1
ENSG00000256683 0.9998519 -4.751339 1 1 1 1
ENSG00000197619 0.9998519 -4.508172 1 1 1 1
ENSG00000142556 0.9998519 -4.809856 1 1 1 1
ENSG00000256087 0.9998519 -4.463165 1 1 1 1
ENSG00000197608 0.9998519 -4.958877 1 1 1 1
ENSG00000204611 0.9998519 -4.955632 1 1 1 1
ENSG00000105568 0.9998519 -5.251971 1 1 1 1
ENSG00000196214 0.9998519 -4.850862 1 1 1 1
ENSG00000198464 0.9998519 -4.384067 1 1 1 1
ENSG00000167554 0.9998519 -4.656716 1 1 1 1
ENSG00000221923 0.9998519 -4.764467 1 1 1 1
ENSG00000167555 0.9998519 -4.807032 1 1 1 1
ENSG00000167766 0.9998519 -4.982285 1 1 1 1
ENSG00000213020 0.9998519 -4.790860 1 1 1 1
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ENSG00000198538 0.9998519 -4.906530 1 1 1 1
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ENSG00000242779 0.9998519 -4.968664 1 1 1 1
ENSG00000170949 0.9998519 -4.891950 1 1 1 1
[ reached 'max' / getOption("max.print") -- omitted 448 rows ]
[[9]]
logFC AveExpr t P.Value
ENSG00000225972 5.568389e-01 3.439734306 3.628821e+00 0.0022211846
ENSG00000225630 2.480504e-01 5.259763521 2.201611e+00 0.0425328119
ENSG00000237973 2.281614e-01 5.765427761 1.988714e+00 0.0639123203
ENSG00000248527 2.958533e-01 7.291375670 2.510478e+00 0.0230392027
ENSG00000198744 -1.482055e-01 1.692163426 -1.102869e+00 0.2862127809
ENSG00000228794 1.484883e-01 2.429990051 9.566100e-01 0.3528352641
ENSG00000223764 -1.157460e-01 1.604113326 -6.839229e-01 0.5036986990
ENSG00000187634 -2.182665e-01 2.774428967 -1.895058e+00 0.0760900049
ENSG00000188976 3.516307e-02 5.602324306 4.734281e-01 0.6422300727
ENSG00000187961 -1.410553e-01 3.205023751 -1.070978e+00 0.2998899465
ENSG00000187608 -6.400174e-02 3.515541854 -4.213422e-01 0.6790473817
ENSG00000188157 -3.424727e-02 8.438003633 -2.953461e-01 0.7714845954
ENSG00000131591 -1.647638e-01 2.698849622 -1.326857e+00 0.2029717020
ENSG00000078808 -6.063881e-02 8.404430472 -8.021385e-01 0.4340927981
ENSG00000176022 -1.142071e-01 5.315643527 -1.170424e+00 0.2587755274
ENSG00000160087 -3.624387e-03 5.574117382 -5.660695e-02 0.9555512131
ENSG00000131584 5.406808e-03 5.338644789 5.996719e-02 0.9529159556
ENSG00000169972 -5.236603e-03 3.649507997 -5.647879e-02 0.9556517355
ENSG00000127054 -9.376932e-03 5.813712471 -1.532963e-01 0.8800595101
ENSG00000224051 -7.337425e-03 4.433660580 -7.541295e-02 0.9408104020
ENSG00000107404 -2.481812e-02 5.541314411 -2.366281e-01 0.8159139889
ENSG00000162576 -7.992954e-02 5.643016003 -6.984197e-01 0.4948279934
ENSG00000175756 -4.843825e-02 5.949578662 -8.752064e-01 0.3942640639
ENSG00000221978 -1.577052e-02 6.117640537 -1.195789e-01 0.9062883870
ENSG00000224870 1.855495e-03 4.193043833 1.862765e-02 0.9853658400
ENSG00000242485 -7.143208e-03 5.474356677 -1.025219e-01 0.9196018484
ENSG00000160072 8.170936e-02 4.020742816 9.752057e-01 0.3438089767
ENSG00000197785 -8.358559e-02 4.667370310 -9.343505e-01 0.3638540775
ENSG00000160075 2.133402e-02 6.698987873 3.759558e-01 0.7118317885
ENSG00000228594 -9.822173e-02 3.231082811 -7.283393e-01 0.4768121082
ENSG00000197530 -3.183417e-04 6.011139637 -2.739815e-03 0.9978474316
ENSG00000189409 3.124193e-03 4.590321361 2.550967e-02 0.9799603030
ENSG00000248333 1.630755e-01 3.904469734 1.632016e+00 0.1219767313
ENSG00000189339 -7.739241e-03 5.329578573 -1.166620e-01 0.9085632269
ENSG00000008130 4.869830e-02 5.472226379 8.229775e-01 0.4224808531
ENSG00000078369 1.446536e-02 8.804321589 2.978826e-01 0.7695824209
ENSG00000162585 1.140936e-03 4.754428869 1.057160e-02 0.9916944498
ENSG00000157933 1.816034e-02 6.562145698 3.003893e-01 0.7677041320
ENSG00000157916 9.026591e-03 7.002652621 1.713345e-01 0.8660844561
ENSG00000157911 -4.581290e-02 3.826112744 -3.829985e-01 0.7067041572
ENSG00000157881 1.530295e-02 4.048444809 1.916296e-01 0.8504157342
ENSG00000157870 -5.118455e-02 3.237377913 -4.060119e-01 0.6900510134
ENSG00000158109 -7.207735e-04 6.732297019 -1.198293e-02 0.9905856948
ENSG00000116213 -2.535484e-02 4.048572327 -3.090923e-01 0.7611945469
ENSG00000227372 6.714475e-03 3.931432132 7.939243e-02 0.9376937967
ENSG00000130764 -3.682471e-02 5.498222486 -5.171777e-01 0.6120273683
ENSG00000116198 8.515316e-02 4.799410870 1.126112e+00 0.2765387110
ENSG00000198912 4.518149e-02 4.517142545 5.902290e-01 0.5631876466
ENSG00000131697 -2.783416e-02 3.217207294 -2.876654e-01 0.7772534664
ENSG00000116251 2.659463e-02 6.491595851 3.670140e-01 0.7183627662
ENSG00000116237 -3.574337e-02 7.144503644 -6.579222e-01 0.5198365643
ENSG00000158292 9.792014e-02 2.850382649 7.152431e-01 0.4846491296
ENSG00000097021 5.090424e-02 4.963811963 2.989705e-01 0.7687670933
ENSG00000215788 1.973834e-01 2.224832337 9.805489e-01 0.3412454824
ENSG00000171680 -5.509493e-02 4.816276482 -3.869577e-01 0.7038278804
ENSG00000162408 -1.508504e-01 3.954485497 -1.509923e+00 0.1503371061
ENSG00000204859 -4.589097e-02 3.319571732 -3.843844e-01 0.7056968341
ENSG00000162413 -7.071694e-03 6.835844141 -1.096389e-01 0.9140436476
ENSG00000116273 -5.812859e-02 5.018894856 -6.599851e-01 0.5185455794
ENSG00000041988 -7.544867e-02 3.387269004 -7.235512e-01 0.4796685712
ENSG00000007923 1.902495e-02 5.116929638 2.107244e-01 0.8357323505
ENSG00000171735 1.377149e-01 3.971476593 1.036645e+00 0.3151414150
ENSG00000049245 -2.307095e-02 6.747961733 -4.333688e-01 0.6704670432
ENSG00000116288 4.113084e-02 7.469041111 7.274157e-01 0.4773623184
ENSG00000116285 1.695913e-01 6.567378923 8.741894e-01 0.3948013549
ENSG00000142599 2.368721e-01 6.882232369 2.694679e+00 0.0158269822
ENSG00000074800 -3.352141e-02 9.240103588 -4.970536e-01 0.6258350545
ENSG00000142583 7.581281e-02 2.154021950 3.052774e-01 0.7640457251
ENSG00000180758 -1.652714e-01 2.520571933 -1.572361e+00 0.1352084223
ENSG00000228526 1.087564e-01 3.111024724 5.227262e-01 0.6082464936
ENSG00000049239 -4.820168e-03 5.843277506 -4.666559e-02 0.9633506910
ENSG00000171621 -6.902721e-02 7.640791271 -7.578026e-01 0.4594609080
ENSG00000171612 -2.095542e-02 2.962339532 -1.511335e-01 0.8817380091
ENSG00000188807 -9.251968e-02 3.161234025 -8.279085e-01 0.4197626065
ENSG00000171608 -4.427091e-02 3.600417471 -3.433655e-01 0.7357430051
ENSG00000171603 -2.982980e-02 7.507456408 -4.239826e-01 0.6771596503
ENSG00000178585 -6.352589e-02 4.096458428 -7.171558e-01 0.4834998298
ENSG00000162441 -5.896161e-02 4.190251149 -6.252478e-01 0.5405243093
ENSG00000173614 1.092395e-01 3.790237230 9.669785e-01 0.3477823715
ENSG00000130939 4.018071e-02 6.013665524 5.880347e-01 0.5646243542
ENSG00000054523 3.108294e-02 6.790885629 5.581488e-01 0.5843815436
ENSG00000142657 -5.748510e-02 6.764910548 -9.806123e-01 0.3412151583
ENSG00000160049 -4.945019e-02 5.436360887 -9.137223e-01 0.3742736362
ENSG00000142655 6.009315e-03 3.921860299 6.900068e-02 0.9458343471
ENSG00000120948 -2.605766e-02 5.498744258 -4.369525e-01 0.6679193130
ENSG00000116649 8.593340e-02 6.989287261 9.866365e-01 0.3383412448
ENSG00000171824 4.382201e-02 5.208139078 6.544118e-01 0.5220375531
ENSG00000198793 1.767341e-02 6.572268484 2.425291e-01 0.8114166765
ENSG00000171819 -6.944477e-02 5.005029463 -3.662898e-01 0.7188927134
ENSG00000120942 -6.916908e-03 4.505427589 -9.170458e-02 0.9280581612
ENSG00000116661 -1.077987e-01 2.256655281 -6.295782e-01 0.5377567854
ENSG00000132879 -1.558534e-01 2.851964046 -1.545874e+00 0.1414619147
ENSG00000116670 -6.695176e-02 3.776766362 -6.858475e-01 0.5025156990
ENSG00000177674 -5.206426e-02 4.748151475 -6.484037e-01 0.5258167627
ENSG00000177000 1.610744e-02 4.306535766 1.860273e-01 0.8547348132
ENSG00000011021 -2.652164e-02 4.716053644 -3.328879e-01 0.7434917353
ENSG00000116685 -4.095967e-02 5.360029702 -5.577215e-01 0.5846665545
ENSG00000083444 -2.842011e-02 9.617689757 -3.604103e-01 0.7232005656
ENSG00000116688 -1.898548e-02 6.632063082 -3.221649e-01 0.7514514375
ENSG00000116691 1.453889e-02 3.486834952 1.727413e-01 0.8649964112
ENSG00000048707 7.056809e-02 5.830304323 1.132089e+00 0.2740905237
ENSG00000162496 -8.611232e-03 3.119770104 -5.484147e-02 0.9569360018
ENSG00000162494 1.294109e-01 1.522400198 7.094685e-01 0.4881289362
ENSG00000162493 3.681934e-02 9.669928769 4.792256e-01 0.6381886947
ENSG00000116731 6.070609e-02 5.589398806 1.090901e+00 0.2912903569
ENSG00000189337 2.396425e-01 3.000052029 1.483583e+00 0.1571306600
ENSG00000171729 -9.056275e-03 3.118331547 -4.502836e-02 0.9646355692
ENSG00000142634 9.531661e-03 4.068304279 6.907069e-02 0.9457794883
ENSG00000132906 -5.584211e-02 3.641839894 -5.840116e-01 0.5672633349
ENSG00000116138 -7.948625e-03 5.209789620 -1.132177e-01 0.9112503584
ENSG00000197312 2.051345e-02 5.118656021 2.374427e-01 0.8152927358
ENSG00000116786 -1.947760e-02 6.306994840 -3.521078e-01 0.7293000430
ENSG00000162458 5.693073e-02 7.121657904 5.566046e-01 0.5854118933
ENSG00000065526 3.576203e-02 5.499653620 5.907424e-01 0.5628518280
ENSG00000116809 -6.001521e-02 4.245851773 -5.767294e-01 0.5720566506
ENSG00000173641 1.440636e-01 5.013884293 8.763761e-01 0.3936467279
ENSG00000142627 -2.707284e-02 6.565606623 -2.399394e-01 0.8133895294
ENSG00000142632 -1.824483e-01 2.972003499 -1.343931e+00 0.1975075655
ENSG00000037637 -6.040867e-02 4.745003107 -8.667195e-01 0.3987624677
ENSG00000055070 3.830017e-02 6.825257519 6.609064e-01 0.5179696112
ENSG00000157191 4.634952e-02 6.110674810 9.645387e-01 0.3489667975
ENSG00000219481 -9.562639e-02 4.648960829 -1.210145e+00 0.2436004086
ENSG00000215908 -1.482044e-01 3.665056004 -1.710134e+00 0.1063378694
ENSG00000058453 -2.109248e-01 4.313812210 -1.845611e+00 0.0833220235
ENSG00000117122 -1.541653e-02 6.989385574 -2.148182e-01 0.8325922832
ENSG00000159363 -1.214343e-02 6.019552468 -1.234974e-01 0.9032338104
ENSG00000117118 -4.641077e-02 6.167713801 -7.754036e-01 0.4492826171
ENSG00000179051 -1.795170e-02 6.193488468 -2.550537e-01 0.8018941178
ENSG00000074964 -1.578633e-02 3.574390645 -1.648273e-01 0.8711208885
ENSG00000159423 -4.799860e-02 2.894230519 -3.530493e-01 0.7286074179
ENSG00000169991 -1.269431e-02 4.732484074 -1.268157e-01 0.9006483694
ENSG00000127481 -9.144598e-02 9.073849311 -1.127538e+00 0.2759529498
ENSG00000127463 3.804123e-03 6.630439259 6.044559e-02 0.9525408201
ENSG00000053372 4.343522e-02 4.612180635 5.518237e-01 0.5886078008
ENSG00000053371 -1.614463e-01 5.024884155 -2.478069e+00 0.0245958356
ENSG00000040487 -6.002672e-02 4.555068328 -5.765681e-01 0.5721630727
ENSG00000077549 -2.292133e-03 7.815775016 -4.834822e-02 0.9620302929
ENSG00000173436 3.936765e-02 3.042210288 3.056573e-01 0.7637616352
ENSG00000158747 -2.955313e-02 6.183331667 -3.181761e-01 0.7544198041
ENSG00000169914 8.194247e-02 4.817885052 6.257160e-01 0.5402247555
ENSG00000188257 1.970280e-01 2.000223706 4.843700e-01 0.6346124143
ENSG00000127472 1.759508e-01 3.088517748 1.315902e+00 0.2065408821
ENSG00000162545 3.323302e-02 4.827178273 2.503226e-01 0.8054875382
ENSG00000090432 1.955467e-02 5.675075630 2.868594e-01 0.7778596637
ENSG00000158828 -1.176944e-01 4.494932010 -1.146946e+00 0.2680765728
ENSG00000117242 1.126598e-01 2.631331799 9.398703e-01 0.3610999101
ENSG00000244038 -2.887474e-03 8.697751526 -4.410647e-02 0.9653591018
ENSG00000117245 8.055869e-02 2.607635834 4.627725e-01 0.6496882113
ENSG00000189410 -6.884761e-02 2.810261770 -4.286306e-01 0.6738419596
ENSG00000127483 2.562719e-02 7.082879913 4.243349e-01 0.6769079543
ENSG00000075151 1.829151e-02 7.195981909 3.332782e-01 0.7432025610
ENSG00000117298 1.424649e-02 7.753644680 2.046426e-01 0.8404026262
ENSG00000142794 4.876303e-02 3.437321097 6.135843e-01 0.5480169628
ENSG00000162551 -3.588406e-02 2.911729077 -2.852511e-01 0.7790696458
ENSG00000090686 -3.470577e-02 6.011313564 -6.331569e-01 0.5354756230
ENSG00000142798 -4.218359e-02 9.621243365 -3.250262e-01 0.7493246423
ENSG00000070831 7.794344e-02 7.015276370 1.198756e+00 0.2478799304
ENSG00000162552 -7.452306e-03 4.263306989 -2.832539e-02 0.9777489441
ENSG00000184677 8.591087e-02 4.911668448 9.148620e-01 0.3736927354
ENSG00000133216 -1.687950e-02 2.966685326 -9.533308e-02 0.9252205895
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ENSG00000105402 2.292760e-02 5.408501710 2.815937e-01 0.7818235104
ENSG00000063169 7.180203e-02 3.327719514 5.263433e-01 0.6057878557
ENSG00000024422 6.361948e-02 8.032530303 7.027797e-01 0.4921780440
ENSG00000105373 -5.330770e-02 5.891610812 -8.836865e-01 0.3898029783
ENSG00000178980 -2.836567e-02 7.035800120 -4.379193e-01 0.6672326499
ENSG00000105499 7.104226e-02 1.927616636 3.158118e-01 0.7561811723
ENSG00000105486 -1.956376e-01 3.002420359 -1.402772e+00 0.1795753098
ENSG00000185453 3.004659e-03 2.708174192 2.383848e-02 0.9812728672
ENSG00000105483 -6.392900e-02 3.977034748 -8.720157e-01 0.3959513272
ENSG00000142227 -2.672635e-02 5.980823546 -2.686313e-01 0.7916072772
ENSG00000105438 8.149159e-03 8.088896974 1.411038e-01 0.8895292133
ENSG00000105464 1.665739e-01 1.667123160 1.198520e+00 0.2479691741
ENSG00000105447 8.849718e-02 4.746674892 1.231046e+00 0.2358948127
ENSG00000105443 -4.681811e-02 5.536496313 -4.685045e-01 0.6456713745
ENSG00000063177 -2.568230e-02 8.145714965 -3.728292e-01 0.7141128553
ENSG00000063176 1.559305e-01 4.421560402 1.652299e+00 0.1177374257
ENSG00000105552 2.682129e-02 3.944928364 3.189702e-01 0.7538285712
ENSG00000087076 -8.126441e-02 4.966516712 -7.249327e-01 0.4788433397
ENSG00000087074 1.668933e-01 6.783914463 2.057871e+00 0.0560809862
ENSG00000104805 1.288592e-02 7.906149214 1.509509e-01 0.8818797371
ENSG00000087088 -5.738324e-02 6.436261320 -5.590632e-01 0.5837718383
ENSG00000087086 -5.174353e-02 12.058941043 -6.743642e-01 0.5095976452
ENSG00000104812 1.639293e-02 5.554919935 2.720587e-01 0.7890168004
ENSG00000183207 1.339974e-02 5.649854617 1.648994e-01 0.8710650630
ENSG00000104852 2.072569e-02 6.570625396 2.060596e-01 0.8393139315
ENSG00000104863 -1.486653e-01 2.860985462 -1.233137e+00 0.2351345255
ENSG00000177380 -6.346102e-02 2.904772987 -4.337635e-01 0.6701862575
ENSG00000130529 -1.745875e-02 3.954710118 -1.285178e-01 0.8993225503
ENSG00000074219 8.397291e-03 4.784127661 1.004123e-01 0.9212502510
ENSG00000104872 -8.868283e-02 5.215611657 -1.146155e+00 0.2683940162
ENSG00000161618 -1.422554e-01 4.073193668 -1.211530e+00 0.2430839128
ENSG00000142541 -3.167126e-02 8.798495572 -5.027931e-01 0.6218820518
ENSG00000142534 4.506815e-02 9.214561396 5.855027e-01 0.5662844624
ENSG00000104870 -1.394604e-01 5.305007871 -1.709065e+00 0.1065396226
ENSG00000142552 4.146683e-02 6.302870351 4.831424e-01 0.6354650338
ENSG00000142546 -2.035014e-03 5.367518939 -2.908462e-02 0.9771527063
ENSG00000126464 -2.481162e-02 4.885551572 -2.464497e-01 0.8084324925
ENSG00000126458 2.754993e-02 6.339176461 4.698978e-01 0.6446966861
ENSG00000126461 3.548075e-02 6.133402435 2.965623e-01 0.7705723624
ENSG00000126456 5.758663e-02 4.969957329 7.385553e-01 0.4707516847
ENSG00000126453 1.473793e-01 2.842914353 1.243673e+00 0.2313318654
ENSG00000126457 5.598592e-02 6.324574391 7.830561e-01 0.4449013126
ENSG00000196961 4.282691e-03 6.862993870 5.105123e-02 0.9599094261
ENSG00000010361 -2.502356e-01 2.746988061 -1.853043e+00 0.0821973999
ENSG00000104973 8.017698e-02 4.644685303 7.496785e-01 0.4642062792
ENSG00000104960 -4.567657e-02 5.126602512 -5.944174e-01 0.5604507845
ENSG00000039650 1.554283e-02 4.318555072 1.806198e-01 0.8589082078
ENSG00000204673 2.043320e-02 6.615684364 2.461301e-01 0.8086756462
ENSG00000104946 3.785559e-02 5.142717077 4.521744e-01 0.6571444655
ENSG00000213024 2.297654e-03 5.178261322 3.606361e-02 0.9716726724
ENSG00000169136 4.944395e-02 3.340949893 4.998240e-01 0.6239254770
ENSG00000105053 -1.119712e-01 4.303539969 -1.087254e+00 0.2928510442
ENSG00000142528 1.425050e-01 3.054946621 1.196725e+00 0.2486490870
ENSG00000131408 -1.255564e-01 5.748430698 -1.589804e+00 0.1312190774
ENSG00000062822 -1.903832e-02 3.000646471 -1.474212e-01 0.8846203247
ENSG00000161671 -7.449825e-02 6.833897974 -1.005200e+00 0.3295925841
ENSG00000161677 -7.438634e-02 4.863914065 -8.611031e-01 0.4017579336
ENSG00000167747 -2.988973e-02 4.345002868 -3.092779e-01 0.7610559347
ENSG00000142544 -1.554391e-01 2.311663930 -6.656117e-01 0.5150336550
ENSG00000105379 1.834916e-01 3.407808788 1.604636e+00 0.1279059325
ENSG00000105497 -1.576406e-02 3.196139230 -1.682965e-01 0.8684350783
ENSG00000182310 8.439719e-04 2.485832085 6.589452e-03 0.9948229507
ENSG00000198093 1.058498e-01 2.053797321 7.450462e-01 0.4669253634
ENSG00000176024 -7.241341e-02 2.592669391 -6.256319e-01 0.5402785580
ENSG00000256683 2.120898e-01 2.455207926 1.828549e+00 0.0859553618
ENSG00000197619 -1.148275e-01 2.482955439 -5.932591e-01 0.5612069708
ENSG00000142556 3.820800e-02 3.382865635 3.543064e-01 0.7276829855
ENSG00000256087 -3.406313e-02 4.021520586 -2.647523e-01 0.7945422120
ENSG00000197608 -1.579575e-01 4.006239358 -1.633831e+00 0.1215921836
ENSG00000204611 4.335509e-02 3.850670783 4.708450e-01 0.6440344701
ENSG00000105568 6.091729e-02 7.439852649 8.546230e-01 0.4052324063
ENSG00000196214 6.328970e-02 3.198031676 6.795799e-01 0.5063739669
ENSG00000198464 -1.185754e-01 3.424444091 -1.121719e+00 0.2783481664
ENSG00000167554 3.701018e-02 2.135684048 2.702501e-01 0.7903834483
ENSG00000221923 6.740501e-02 2.348356875 5.171583e-01 0.6120406042
ENSG00000167555 1.165429e-01 2.377642494 9.988522e-01 0.3325659337
ENSG00000167766 4.478686e-02 3.897495780 4.527314e-01 0.6567516665
ENSG00000213020 -1.131545e-01 2.480214607 -9.759210e-01 0.3434650150
ENSG00000189190 -7.818841e-03 1.940346670 -4.124230e-02 0.9676071963
ENSG00000198538 -5.175959e-02 3.912907898 -4.412022e-01 0.6649034437
ENSG00000204604 -1.401986e-01 4.547968222 -1.118208e+00 0.2798005761
ENSG00000213793 1.616135e-01 3.335171849 1.335723e+00 0.2001194635
ENSG00000180257 -1.176918e-02 3.077122991 -1.175510e-01 0.9078697762
ENSG00000242779 1.104585e-01 4.041796100 1.464984e+00 0.1620797823
ENSG00000170949 -9.444381e-02 4.027401106 -1.296540e+00 0.2129704014
adj.P.Val B lfsr lfdr qvalue svalue
ENSG00000225972 0.9997370 -2.5092096 1 1 1 1
ENSG00000225630 0.9997370 -3.7362668 1 1 1 1
ENSG00000237973 0.9997370 -3.9394002 1 1 1 1
ENSG00000248527 0.9997370 -3.3259548 1 1 1 1
ENSG00000198744 0.9997370 -4.5954422 1 1 1 1
ENSG00000228794 0.9997370 -4.6341922 1 1 1 1
ENSG00000223764 0.9997370 -4.7092965 1 1 1 1
ENSG00000187634 0.9997370 -4.1697680 1 1 1 1
ENSG00000188976 0.9997370 -5.0683421 1 1 1 1
ENSG00000187961 0.9997370 -4.6204218 1 1 1 1
ENSG00000187608 0.9997370 -4.8736044 1 1 1 1
ENSG00000188157 0.9997370 -5.1358067 1 1 1 1
ENSG00000131591 0.9997370 -4.5107391 1 1 1 1
ENSG00000078808 0.9997370 -4.9416765 1 1 1 1
ENSG00000176022 0.9997370 -4.6740054 1 1 1 1
ENSG00000160087 0.9997370 -5.1426109 1 1 1 1
ENSG00000131584 0.9997370 -5.1205818 1 1 1 1
ENSG00000169972 0.9997370 -4.9207971 1 1 1 1
ENSG00000127054 0.9997370 -5.1521933 1 1 1 1
ENSG00000224051 0.9997370 -5.0119591 1 1 1 1
ENSG00000107404 0.9997370 -5.1103210 1 1 1 1
ENSG00000162576 0.9997370 -4.9726825 1 1 1 1
ENSG00000175756 0.9997370 -4.8981685 1 1 1 1
ENSG00000221978 0.9997370 -5.1761646 1 1 1 1
ENSG00000224870 0.9997370 -4.9973793 1 1 1 1
ENSG00000242485 0.9997370 -5.1385863 1 1 1 1
ENSG00000160072 0.9997370 -4.7159543 1 1 1 1
ENSG00000197785 0.9997370 -4.7836178 1 1 1 1
ENSG00000160075 0.9997370 -5.1422018 1 1 1 1
ENSG00000228594 0.9997370 -4.7502292 1 1 1 1
ENSG00000197530 0.9999118 -5.1731461 1 1 1 1
ENSG00000189409 0.9997370 -5.0322293 1 1 1 1
ENSG00000248333 0.9997370 -4.3095059 1 1 1 1
ENSG00000189339 0.9997370 -5.1205794 1 1 1 1
ENSG00000008130 0.9997370 -4.9028526 1 1 1 1
ENSG00000078369 0.9997370 -5.1265268 1 1 1 1
ENSG00000162585 0.9997370 -5.0521160 1 1 1 1
ENSG00000157933 0.9997370 -5.1595651 1 1 1 1
ENSG00000157916 0.9997370 -5.1855341 1 1 1 1
ENSG00000157911 0.9997370 -4.9089414 1 1 1 1
ENSG00000157881 0.9997370 -4.9603274 1 1 1 1
ENSG00000157870 0.9997370 -4.8280841 1 1 1 1
ENSG00000158109 0.9997370 -5.1945494 1 1 1 1
ENSG00000116213 0.9997370 -4.9430588 1 1 1 1
ENSG00000227372 0.9997370 -4.9483443 1 1 1 1
ENSG00000130764 0.9997370 -5.0451881 1 1 1 1
ENSG00000116198 0.9997370 -4.6751931 1 1 1 1
ENSG00000198912 0.9997370 -4.9221847 1 1 1 1
ENSG00000131697 0.9997370 -4.8482952 1 1 1 1
ENSG00000116251 0.9997370 -5.1434585 1 1 1 1
ENSG00000116237 0.9997370 -5.0384090 1 1 1 1
ENSG00000158292 0.9997370 -4.7162044 1 1 1 1
ENSG00000097021 0.9997370 -5.0314141 1 1 1 1
ENSG00000215788 0.9997370 -4.6313572 1 1 1 1
ENSG00000171680 0.9997370 -5.0418523 1 1 1 1
ENSG00000162408 0.9997370 -4.3913628 1 1 1 1
ENSG00000204859 0.9997370 -4.8419603 1 1 1 1
ENSG00000162413 0.9997370 -5.1910786 1 1 1 1
ENSG00000116273 0.9997370 -4.9377750 1 1 1 1
ENSG00000041988 0.9997370 -4.7596314 1 1 1 1
ENSG00000007923 0.9997370 -5.0912003 1 1 1 1
ENSG00000171735 0.9997370 -4.6745103 1 1 1 1
ENSG00000049245 0.9997370 -5.1250677 1 1 1 1
ENSG00000116288 0.9997370 -5.0021928 1 1 1 1
ENSG00000116285 0.9997370 -4.9002955 1 1 1 1
ENSG00000142599 0.9997370 -3.1013738 1 1 1 1
ENSG00000074800 0.9997370 -5.0569462 1 1 1 1
ENSG00000142583 0.9997370 -4.7240057 1 1 1 1
ENSG00000180758 0.9997370 -4.4193087 1 1 1 1
ENSG00000228526 0.9997370 -4.7893534 1 1 1 1
ENSG00000049239 0.9997370 -5.1650225 1 1 1 1
ENSG00000171621 0.9997370 -4.9814668 1 1 1 1
ENSG00000171612 0.9997370 -4.8281845 1 1 1 1
ENSG00000188807 0.9997370 -4.7133688 1 1 1 1
ENSG00000171608 0.9997370 -4.8872051 1 1 1 1
ENSG00000171603 0.9997370 -5.1278879 1 1 1 1
ENSG00000178585 0.9997370 -4.8392552 1 1 1 1
ENSG00000162441 0.9997370 -4.8717331 1 1 1 1
ENSG00000173614 0.9997370 -4.7048547 1 1 1 1
ENSG00000130939 0.9997370 -5.0511739 1 1 1 1
ENSG00000054523 0.9997370 -5.0815400 1 1 1 1
ENSG00000142657 0.9997370 -4.8507830 1 1 1 1
ENSG00000160049 0.9997370 -4.8495967 1 1 1 1
ENSG00000142655 0.9997370 -4.9490734 1 1 1 1
ENSG00000120948 0.9997370 -5.0744940 1 1 1 1
ENSG00000116649 0.9997370 -4.8480551 1 1 1 1
ENSG00000171824 0.9997370 -4.9652967 1 1 1 1
ENSG00000198793 0.9997370 -5.1724347 1 1 1 1
ENSG00000171819 0.9997370 -5.0838040 1 1 1 1
ENSG00000120942 0.9997370 -5.0406527 1 1 1 1
ENSG00000116661 0.9997370 -4.7150251 1 1 1 1
ENSG00000132879 0.9997370 -4.4080488 1 1 1 1
ENSG00000116670 0.9997370 -4.8154947 1 1 1 1
ENSG00000177674 0.9997370 -4.9113797 1 1 1 1
ENSG00000177000 0.9997370 -4.9924405 1 1 1 1
ENSG00000011021 0.9997370 -5.0246554 1 1 1 1
ENSG00000116685 0.9997370 -5.0220079 1 1 1 1
ENSG00000083444 0.9997370 -5.0744856 1 1 1 1
ENSG00000116688 0.9997370 -5.1557675 1 1 1 1
ENSG00000116691 0.9997370 -4.8718496 1 1 1 1
ENSG00000048707 0.9997370 -4.7245828 1 1 1 1
ENSG00000162496 0.9997370 -4.8506984 1 1 1 1
ENSG00000162494 0.9997370 -4.6730502 1 1 1 1
ENSG00000162493 0.9997370 -5.0452940 1 1 1 1
ENSG00000116731 0.9997370 -4.7449236 1 1 1 1
ENSG00000189337 0.9997370 -4.4426349 1 1 1 1
ENSG00000171729 0.9997370 -4.8366920 1 1 1 1
ENSG00000142634 0.9997370 -4.9509651 1 1 1 1
ENSG00000132906 0.9997370 -4.8419097 1 1 1 1
ENSG00000116138 0.9997370 -5.1146725 1 1 1 1
ENSG00000197312 0.9997370 -5.0805152 1 1 1 1
ENSG00000116786 0.9997370 -5.1391998 1 1 1 1
ENSG00000162458 0.9997370 -5.0823068 1 1 1 1
ENSG00000065526 0.9997370 -5.0184959 1 1 1 1
ENSG00000116809 0.9997370 -4.8882862 1 1 1 1
ENSG00000173641 0.9997370 -4.8263802 1 1 1 1
ENSG00000142627 0.9997370 -5.1639354 1 1 1 1
ENSG00000142632 0.9997370 -4.4985056 1 1 1 1
ENSG00000037637 0.9997370 -4.8267812 1 1 1 1
ENSG00000055070 0.9997370 -5.0358148 1 1 1 1
ENSG00000157191 0.9997370 -4.8502950 1 1 1 1
ENSG00000219481 0.9997370 -4.6102537 1 1 1 1
ENSG00000215908 0.9997370 -4.2673419 1 1 1 1
ENSG00000058453 0.9997370 -4.1276267 1 1 1 1
ENSG00000117122 0.9997370 -5.1768821 1 1 1 1
ENSG00000159363 0.9997370 -5.1695323 1 1 1 1
ENSG00000117118 0.9997370 -4.9660393 1 1 1 1
ENSG00000179051 0.9997370 -5.1614695 1 1 1 1
ENSG00000074964 0.9997370 -4.9008181 1 1 1 1
ENSG00000159423 0.9997370 -4.8154298 1 1 1 1
ENSG00000169991 0.9997370 -5.0640977 1 1 1 1
ENSG00000127481 0.9997370 -4.7159752 1 1 1 1
ENSG00000127463 0.9997370 -5.1929071 1 1 1 1
ENSG00000053372 0.9997370 -4.9447053 1 1 1 1
ENSG00000053371 0.9997370 -3.4454776 1 1 1 1
ENSG00000040487 0.9997370 -4.9303220 1 1 1 1
ENSG00000077549 0.9997370 -5.1879489 1 1 1 1
ENSG00000173436 0.9997370 -4.8236816 1 1 1 1
ENSG00000158747 0.9997370 -5.1478537 1 1 1 1
ENSG00000169914 0.9997370 -4.9268108 1 1 1 1
ENSG00000188257 0.9997370 -4.7111138 1 1 1 1
ENSG00000127472 0.9997370 -4.5123614 1 1 1 1
ENSG00000162545 0.9997370 -5.0509171 1 1 1 1
ENSG00000090432 0.9997370 -5.1255500 1 1 1 1
ENSG00000158828 0.9997370 -4.6458216 1 1 1 1
ENSG00000117242 0.9997370 -4.6517963 1 1 1 1
ENSG00000244038 0.9997370 -5.1580879 1 1 1 1
ENSG00000117245 0.9997370 -4.7553336 1 1 1 1
ENSG00000189410 0.9997370 -4.7761981 1 1 1 1
ENSG00000127483 0.9997370 -5.1305933 1 1 1 1
ENSG00000075151 0.9997370 -5.1556920 1 1 1 1
ENSG00000117298 0.9997370 -5.1756799 1 1 1 1
ENSG00000142794 0.9997370 -4.8029455 1 1 1 1
ENSG00000162551 0.9997370 -4.8457196 1 1 1 1
ENSG00000090686 0.9997370 -5.0342408 1 1 1 1
ENSG00000142798 0.9997370 -5.0890163 1 1 1 1
ENSG00000070831 0.9997370 -4.6916797 1 1 1 1
ENSG00000162552 0.9997370 -5.0441944 1 1 1 1
ENSG00000184677 0.9997370 -4.8111924 1 1 1 1
ENSG00000133216 0.9997370 -4.8318143 1 1 1 1
ENSG00000004487 0.9997370 -4.5217029 1 1 1 1
ENSG00000169641 0.9997370 -5.1507156 1 1 1 1
ENSG00000179546 0.9997370 -4.3578234 1 1 1 1
ENSG00000125944 0.9997370 -5.1711605 1 1 1 1
ENSG00000125945 0.9997370 -4.8727314 1 1 1 1
ENSG00000088280 0.9997370 -4.8797818 1 1 1 1
ENSG00000117318 0.9997370 -5.1570931 1 1 1 1
ENSG00000142676 0.9997370 -5.1670344 1 1 1 1
ENSG00000011007 0.9997370 -5.1814527 1 1 1 1
ENSG00000057757 0.9997370 -5.1170107 1 1 1 1
ENSG00000011009 0.9997370 -4.6338906 1 1 1 1
ENSG00000117308 0.9997370 -4.9901517 1 1 1 1
ENSG00000117305 0.9997370 -4.9518901 1 1 1 1
ENSG00000179163 0.9997370 -4.9494637 1 1 1 1
ENSG00000189266 0.9997370 -4.5688015 1 1 1 1
ENSG00000188529 0.9997370 -5.0917302 1 1 1 1
ENSG00000001460 0.9997370 -4.7420188 1 1 1 1
ENSG00000001461 0.9997370 -5.1939708 1 1 1 1
ENSG00000117602 0.9997370 -4.8536473 1 1 1 1
ENSG00000133226 0.9997370 -5.0493011 1 1 1 1
ENSG00000169504 0.9997370 -5.0279425 1 1 1 1
ENSG00000117614 0.9997370 -5.0883653 1 1 1 1
ENSG00000117616 0.9997370 -4.6893036 1 1 1 1
ENSG00000183726 0.9997370 -5.1198645 1 1 1 1
ENSG00000204178 0.9997370 -5.1642384 1 1 1 1
ENSG00000157978 0.9997370 -5.1779483 1 1 1 1
ENSG00000117643 0.9997370 -4.6662492 1 1 1 1
ENSG00000162430 0.9997370 -4.1103506 1 1 1 1
ENSG00000117640 0.9997370 -4.7850424 1 1 1 1
ENSG00000182749 0.9997370 -4.9191031 1 1 1 1
ENSG00000117632 0.9997370 -5.1189444 1 1 1 1
ENSG00000158006 0.9997370 -4.9325929 1 1 1 1
ENSG00000158008 0.9997370 -4.7555983 1 1 1 1
ENSG00000158022 0.9997370 -4.6166157 1 1 1 1
ENSG00000130695 0.9997370 -4.4577894 1 1 1 1
ENSG00000142669 0.9997370 -4.8653404 1 1 1 1
ENSG00000158062 0.9997370 -4.7445077 1 1 1 1
ENSG00000117682 0.9997370 -5.1715152 1 1 1 1
ENSG00000198830 0.9997370 -5.0276579 1 1 1 1
ENSG00000117713 0.9997370 -5.1167148 1 1 1 1
ENSG00000060642 0.9997370 -4.7002330 1 1 1 1
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ENSG00000142751 0.9997370 -4.2086146 1 1 1 1
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ENSG00000253368 0.9997370 -5.0034856 1 1 1 1
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ENSG00000126705 0.9997370 -5.1179930 1 1 1 1
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ENSG00000009780 0.9997370 -4.6801329 1 1 1 1
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[ reached 'max' / getOption("max.print") -- omitted 448 rows ]
[[10]]
logFC AveExpr t P.Value
ENSG00000225972 -3.277380e-01 3.439734306 -1.119568e+00 0.2792039386
ENSG00000225630 4.387818e-02 5.259763521 3.106782e-01 0.7600022314
ENSG00000237973 2.549907e-02 5.765427761 1.618108e-01 0.8734534507
ENSG00000248527 1.832106e-02 7.291375670 1.167300e-01 0.9085072058
ENSG00000198744 1.224466e-01 1.692163426 1.143530e+00 0.2694167922
ENSG00000228794 -3.244804e-01 2.429990051 -1.909820e+00 0.0740049119
ENSG00000223764 4.344052e-01 1.604113326 2.324032e+00 0.0334223610
ENSG00000187634 2.465307e-01 2.774428967 1.563564e+00 0.1372195317
ENSG00000188976 1.180504e-02 5.602324306 1.370154e-01 0.8927050455
ENSG00000187961 1.844449e-01 3.205023751 1.224155e+00 0.2383787588
ENSG00000187608 2.455370e-01 3.515541854 1.363316e+00 0.1914093408
ENSG00000188157 1.592253e-01 8.438003633 1.271425e+00 0.2215083252
ENSG00000131591 1.847005e-01 2.698849622 1.283493e+00 0.2173549433
ENSG00000078808 2.915220e-02 8.404430472 3.343325e-01 0.7424128868
ENSG00000176022 9.196119e-02 5.315643527 8.041677e-01 0.4329295705
ENSG00000160087 3.857852e-02 5.574117382 5.295648e-01 0.6035875365
ENSG00000131584 8.535390e-02 5.338644789 8.397950e-01 0.4132315091
ENSG00000169972 4.707302e-02 3.649507997 4.418212e-01 0.6644527457
ENSG00000127054 6.847624e-02 5.813712471 1.009063e+00 0.3277619426
ENSG00000224051 -6.692329e-02 4.433660580 -5.999300e-01 0.5568424096
ENSG00000107404 -8.765204e-03 5.541314411 -7.217000e-02 0.9433490987
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ENSG00000167555 1.713247e-01 2.377642494 1.003703e+00 0.3302619354
ENSG00000167766 4.129026e-02 3.897495780 3.665583e-01 0.7186865036
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ENSG00000198538 -1.497041e-01 3.912907898 -1.151390e+00 0.2662628166
ENSG00000204604 1.278701e-01 4.547968222 9.475637e-01 0.3572568822
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ENSG00000180257 1.379998e-02 3.077122991 1.186289e-01 0.9070261704
ENSG00000242779 -3.505471e-04 4.041796100 -4.157299e-03 0.9967336700
ENSG00000170949 1.457758e-01 4.027401106 1.884510e+00 0.0775463574
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[ reached 'max' / getOption("max.print") -- omitted 448 rows ]
[[11]]
logFC AveExpr t P.Value
ENSG00000225972 -2.062813e-01 3.439734306 -7.968613e-01 4.371140e-01
ENSG00000225630 -1.579456e-01 5.259763521 -1.093937e+00 2.900637e-01
ENSG00000237973 -3.427893e-01 5.765427761 -2.597438e+00 1.935984e-02
ENSG00000248527 -2.431981e-01 7.291375670 -1.593777e+00 1.304067e-01
ENSG00000198744 1.223492e-01 1.692163426 8.843696e-01 3.894998e-01
ENSG00000228794 -2.844346e-02 2.429990051 -1.503906e-01 8.823226e-01
ENSG00000223764 -1.484227e-02 1.604113326 -6.415398e-02 9.496366e-01
ENSG00000187634 1.562791e-01 2.774428967 9.623578e-01 3.500878e-01
ENSG00000188976 3.485969e-02 5.602324306 3.950751e-01 6.979673e-01
ENSG00000187961 2.745835e-01 3.205023751 1.890268e+00 7.684332e-02
ENSG00000187608 2.403950e-01 3.515541854 1.339219e+00 1.990819e-01
ENSG00000188157 1.991143e-01 8.438003633 1.564796e+00 1.370523e-01
ENSG00000131591 2.147105e-01 2.698849622 1.439257e+00 1.692175e-01
ENSG00000078808 9.709187e-02 8.404430472 1.115342e+00 2.810591e-01
ENSG00000176022 1.746320e-01 5.315643527 1.541749e+00 1.425394e-01
ENSG00000160087 -1.207632e-02 5.574117382 -1.602312e-01 8.746900e-01
ENSG00000131584 1.331853e-01 5.338644789 1.304019e+00 2.105455e-01
ENSG00000169972 4.608736e-02 3.649507997 4.204368e-01 6.797188e-01
ENSG00000127054 6.799109e-02 5.813712471 9.730258e-01 3.449192e-01
ENSG00000224051 -1.500716e-02 4.433660580 -1.312551e-01 8.971980e-01
ENSG00000107404 1.264389e-01 5.541314411 1.068110e+00 3.012095e-01
ENSG00000162576 1.824176e-01 5.643016003 1.420777e+00 1.744404e-01
ENSG00000175756 5.616559e-02 5.949578662 8.638322e-01 4.003540e-01
ENSG00000221978 1.444939e-01 6.117640537 9.423921e-01 3.599050e-01
ENSG00000224870 6.974735e-02 4.193043833 5.940572e-01 5.607208e-01
ENSG00000242485 -3.466007e-02 5.474356677 -4.207007e-01 6.795299e-01
ENSG00000160072 3.952831e-02 4.020742816 3.834583e-01 7.063911e-01
ENSG00000197785 1.072823e-01 4.667370310 1.011342e+00 3.267963e-01
ENSG00000160075 -1.644480e-02 6.698987873 -2.466040e-01 8.083283e-01
ENSG00000228594 1.733650e-01 3.231082811 1.114502e+00 2.814087e-01
ENSG00000197530 2.172745e-01 6.011139637 1.711841e+00 1.060981e-01
ENSG00000189409 1.116679e-01 4.590321361 7.977088e-01 4.366359e-01
ENSG00000248333 -2.602572e-02 3.904469734 -2.056393e-01 8.396477e-01
ENSG00000189339 5.616034e-02 5.329578573 7.279983e-01 4.770593e-01
ENSG00000008130 2.895002e-02 5.472226379 4.119645e-01 6.857927e-01
ENSG00000078369 2.403209e-03 8.804321589 4.229427e-02 9.667837e-01
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ENSG00000263002 -4.788099e-02 3.408531106 -3.255454e-01 7.489566e-01
ENSG00000167380 -2.416612e-02 4.441687498 -2.010592e-01 8.431678e-01
ENSG00000131115 -1.564024e-01 2.992248604 -1.464549e+00 1.622780e-01
ENSG00000062370 -1.691993e-01 2.401433710 -9.471983e-01 3.575246e-01
ENSG00000278318 -1.684600e-01 0.979126751 -1.361725e+00 1.920177e-01
ENSG00000167384 3.926050e-02 2.427775316 2.563626e-01 8.009146e-01
ENSG00000073008 2.668956e-02 6.628771200 3.935653e-01 6.990598e-01
ENSG00000279095 -1.377288e-02 2.586428111 -8.185373e-02 9.357710e-01
ENSG00000069399 5.218814e-01 3.339772580 3.496282e+00 2.959279e-03
ENSG00000130202 1.947879e-02 6.894434617 2.036381e-01 8.411853e-01
ENSG00000130204 6.834098e-02 4.659605433 6.938279e-01 4.976695e-01
ENSG00000130203 7.903727e-02 3.143686136 4.709800e-01 6.439669e-01
ENSG00000104853 1.574920e-02 6.993213285 2.173698e-01 8.306482e-01
ENSG00000104856 -1.940087e-01 2.766145266 -7.580625e-01 4.593558e-01
ENSG00000104859 4.343074e-02 4.072995944 4.367418e-01 6.680935e-01
ENSG00000142252 -1.065522e-02 3.467358598 -1.073147e-01 9.158639e-01
ENSG00000007047 3.111919e-02 5.363544183 2.866603e-01 7.780249e-01
ENSG00000104866 1.019097e-01 4.825681576 9.735966e-01 3.446441e-01
ENSG00000007255 2.445507e-01 2.891837829 1.235907e+00 2.342045e-01
ENSG00000189114 -5.704716e-02 4.089487234 -5.670577e-01 5.784882e-01
ENSG00000104884 4.275923e-02 4.719206848 3.438843e-01 7.353790e-01
ENSG00000104881 5.662244e-03 4.899760551 3.879544e-02 9.695300e-01
ENSG00000012061 -1.472379e-01 5.266235979 -1.327058e+00 2.029846e-01
ENSG00000125744 1.757760e-03 3.270864718 1.459496e-02 9.885345e-01
ENSG00000125753 -2.284557e-02 6.542329712 -2.161108e-01 8.316130e-01
ENSG00000125741 -1.833352e-02 5.027972167 -2.225790e-01 8.266594e-01
ENSG00000125746 6.842313e-03 4.067216554 7.331907e-02 9.424545e-01
ENSG00000125743 -1.048878e-01 6.918742564 -1.430776e+00 1.715982e-01
ENSG00000011478 6.496225e-02 5.400119293 8.672543e-01 3.985316e-01
ENSG00000177051 4.391583e-02 2.991101820 2.723665e-01 7.887989e-01
ENSG00000177045 3.783068e-01 3.224570072 1.817333e+00 8.780610e-02
ENSG00000104936 1.736511e-01 5.370633599 1.890349e+00 7.683183e-02
ENSG00000185800 1.274370e-01 5.023702953 1.287245e+00 2.161892e-01
ENSG00000125755 1.315033e-01 6.003761254 1.481377e+00 1.577925e-01
ENSG00000170604 7.002524e-02 4.613920159 4.246050e-01 6.767388e-01
ENSG00000176182 5.751388e-02 3.618458128 4.654277e-01 6.478525e-01
ENSG00000188624 2.838200e-01 2.451040156 1.952247e+00 6.850469e-02
ENSG00000204866 9.176680e-02 6.246041858 9.012372e-01 3.807332e-01
ENSG00000124440 -7.731658e-02 3.202673522 -4.294210e-01 6.733025e-01
ENSG00000011485 -1.567618e-01 5.531932188 -1.583280e+00 1.327816e-01
ENSG00000169515 1.319402e-01 6.217376588 1.401880e+00 1.799166e-01
ENSG00000182013 1.258815e-03 4.098389913 1.134540e-02 9.910871e-01
ENSG00000160014 1.825858e-02 6.669841601 2.782166e-01 7.843839e-01
ENSG00000197380 3.156457e-01 1.871194780 1.352938e+00 1.947514e-01
ENSG00000105287 -9.882031e-02 3.771143271 -7.234789e-01 4.797556e-01
ENSG00000090372 6.043316e-02 5.820892491 7.338612e-01 4.735750e-01
ENSG00000181027 9.075127e-02 4.641894417 7.702409e-01 4.523005e-01
ENSG00000105281 2.615462e-02 7.857433925 3.428357e-01 7.361529e-01
ENSG00000042753 -3.200671e-02 7.434923049 -4.998863e-01 6.239111e-01
ENSG00000160007 1.904094e-02 6.288965066 2.591721e-01 7.987837e-01
ENSG00000130751 2.750847e-01 1.595824281 8.689163e-01 3.976486e-01
ENSG00000130748 3.371120e-01 3.344774900 1.988065e+00 6.406535e-02
ENSG00000130749 -3.351755e-02 4.763281284 -2.527368e-01 8.036668e-01
ENSG00000142230 -1.959728e-02 6.729612621 -2.802756e-01 7.828318e-01
ENSG00000105327 1.948407e-01 3.801955028 1.327619e+00 2.028032e-01
ENSG00000105321 9.387848e-02 4.678268012 8.880875e-01 3.875561e-01
ENSG00000257704 1.485598e-01 3.298874371 8.717602e-01 3.961406e-01
ENSG00000134815 9.793041e-02 3.693557527 7.737966e-01 4.502533e-01
ENSG00000105419 1.944899e-01 4.933703137 1.996036e+00 6.311348e-02
ENSG00000105402 -3.509999e-03 5.408501710 -3.599910e-02 9.717252e-01
ENSG00000063169 6.269048e-02 3.327719514 3.947098e-01 6.982316e-01
ENSG00000024422 -7.058970e-02 8.032530303 -6.576690e-01 5.200344e-01
ENSG00000105373 9.528716e-04 5.891610812 1.324959e-02 9.895913e-01
ENSG00000178980 4.503409e-03 7.035800120 5.853140e-02 9.540449e-01
ENSG00000105499 -2.196779e-03 1.927616636 -9.154586e-03 9.928082e-01
ENSG00000105486 2.832343e-01 3.002420359 1.747426e+00 9.958705e-02
ENSG00000185453 1.826445e-01 2.708174192 1.323504e+00 2.041366e-01
ENSG00000105483 5.904341e-02 3.977034748 6.747993e-01 5.093687e-01
ENSG00000142227 -4.478493e-02 5.980823546 -3.865146e-01 7.041710e-01
ENSG00000105438 -1.672522e-02 8.088896974 -2.481210e-01 8.071746e-01
ENSG00000105464 -2.178903e-02 1.667123160 -1.321007e-01 8.965398e-01
ENSG00000105447 -5.163345e-02 4.746674892 -5.958021e-01 5.595825e-01
ENSG00000105443 1.791365e-01 5.536496313 1.612353e+00 1.262927e-01
ENSG00000063177 5.168998e-02 8.145714965 6.410970e-01 5.304714e-01
ENSG00000063176 -1.556378e-02 4.421560402 -1.280323e-01 8.997074e-01
ENSG00000105552 4.843294e-02 3.944928364 4.968249e-01 6.260212e-01
ENSG00000087076 -1.094911e-03 4.966516712 -8.451363e-03 9.933606e-01
ENSG00000087074 -2.556687e-02 6.783914463 -2.374844e-01 8.152737e-01
ENSG00000104805 7.910012e-02 7.906149214 8.134207e-01 4.278312e-01
ENSG00000087088 2.694500e-01 6.436261320 2.631115e+00 1.807554e-02
ENSG00000087086 8.911156e-02 12.058941043 1.001463e+00 3.314032e-01
ENSG00000104812 8.524824e-02 5.554919935 1.250744e+00 2.288818e-01
ENSG00000183207 2.280460e-02 5.649854617 2.376776e-01 8.151264e-01
ENSG00000104852 1.865624e-01 6.570625396 1.694944e+00 1.093171e-01
ENSG00000104863 2.243790e-01 2.860985462 1.560019e+00 1.381748e-01
ENSG00000177380 3.934315e-01 2.904772987 2.598878e+00 1.930318e-02
ENSG00000130529 6.986503e-02 3.954710118 4.319463e-01 6.715037e-01
ENSG00000074219 1.556818e-01 4.784127661 1.677648e+00 1.126994e-01
ENSG00000104872 3.348059e-02 5.215611657 3.531052e-01 7.285857e-01
ENSG00000161618 1.158232e-01 4.073193668 7.863066e-01 4.430965e-01
ENSG00000142541 -1.261682e-02 8.798495572 -1.695704e-01 8.674582e-01
ENSG00000142534 -5.516253e-02 9.214561396 -6.082012e-01 5.515298e-01
ENSG00000104870 1.031474e-01 5.305007871 1.009117e+00 3.278300e-01
ENSG00000142552 -3.033763e-03 6.302870351 -3.088083e-02 9.757438e-01
ENSG00000142546 -1.125937e-01 5.367518939 -1.439740e+00 1.690827e-01
ENSG00000126464 1.038345e-01 4.885551572 8.993223e-01 3.817217e-01
ENSG00000126458 5.559530e-02 6.339176461 8.201837e-01 4.240764e-01
ENSG00000126461 1.550978e-01 6.133402435 1.131039e+00 2.745890e-01
ENSG00000126456 5.262915e-02 4.969957329 5.675008e-01 5.781943e-01
ENSG00000126453 -1.278543e-01 2.842914353 -8.727072e-01 3.956392e-01
ENSG00000126457 -1.391611e-02 6.324574391 -1.617754e-01 8.734935e-01
ENSG00000196961 6.403957e-02 6.862993870 6.539684e-01 5.223549e-01
ENSG00000010361 6.642158e-02 2.746988061 3.875413e-01 7.034258e-01
ENSG00000104973 -5.332447e-02 4.644685303 -4.168653e-01 6.822764e-01
ENSG00000104960 9.642665e-02 5.126602512 1.088247e+00 2.924927e-01
ENSG00000039650 1.929541e-02 4.318555072 1.886367e-01 8.527325e-01
ENSG00000204673 1.000342e-01 6.615684364 1.051337e+00 3.086132e-01
ENSG00000104946 9.933460e-02 5.142717077 1.033027e+00 3.168450e-01
ENSG00000213024 9.349429e-02 5.178261322 1.314441e+00 2.070979e-01
ENSG00000169136 1.685658e-01 3.340949893 1.425770e+00 1.730164e-01
ENSG00000105053 -3.043226e-02 4.303539969 -2.459593e-01 8.088187e-01
ENSG00000142528 -9.568706e-02 3.054946621 -6.894014e-01 5.003770e-01
ENSG00000131408 2.374984e-01 5.748430698 2.944010e+00 9.472148e-03
ENSG00000062822 1.964351e-01 3.000646471 1.347694e+00 1.963976e-01
ENSG00000161671 1.251079e-01 6.833897974 1.479401e+00 1.583137e-01
ENSG00000161677 1.503106e-01 4.863914065 1.524760e+00 1.467014e-01
ENSG00000167747 1.143297e-01 4.345002868 1.036649e+00 3.152041e-01
ENSG00000142544 3.381991e-01 2.311663930 1.231436e+00 2.358271e-01
ENSG00000105379 -6.932273e-02 3.407808788 -4.713594e-01 6.437017e-01
ENSG00000105497 5.674069e-03 3.196139230 5.059379e-02 9.602710e-01
ENSG00000182310 8.230159e-02 2.485832085 5.365572e-01 5.989026e-01
ENSG00000198093 -2.410902e-01 2.053797321 -1.412125e+00 1.769304e-01
ENSG00000176024 1.943566e-01 2.592669391 1.535314e+00 1.441040e-01
ENSG00000256683 -2.641410e-01 2.455207926 -1.890706e+00 7.678125e-02
ENSG00000197619 -8.191555e-02 2.482955439 -3.576203e-01 7.252678e-01
ENSG00000142556 -7.822893e-02 3.382865635 -6.097679e-01 5.505167e-01
ENSG00000256087 6.244081e-02 4.021520586 4.102505e-01 6.870242e-01
ENSG00000197608 1.715010e-01 4.006239358 1.458626e+00 1.638816e-01
ENSG00000204611 -4.440526e-02 3.850670783 -4.041174e-01 6.914384e-01
ENSG00000105568 3.437991e-02 7.439852649 4.027451e-01 6.924276e-01
ENSG00000196214 -6.490260e-02 3.198031676 -5.822126e-01 5.684796e-01
ENSG00000198464 5.043357e-02 3.424444091 3.825927e-01 7.070204e-01
ENSG00000167554 -1.246502e-01 2.135684048 -7.470227e-01 4.658094e-01
ENSG00000221923 -2.376670e-01 2.348356875 -1.592612e+00 1.306685e-01
ENSG00000167555 -3.769011e-02 2.377642494 -2.399521e-01 8.133927e-01
ENSG00000167766 -6.935445e-02 3.897495780 -5.937140e-01 5.609448e-01
ENSG00000213020 1.754035e-02 2.480214607 1.258655e-01 9.013952e-01
ENSG00000189190 3.256262e-01 1.940346670 1.678098e+00 1.126103e-01
ENSG00000198538 -1.131020e-01 3.912907898 -8.410969e-01 4.125997e-01
ENSG00000204604 -1.068317e-01 4.547968222 -7.686167e-01 4.532376e-01
ENSG00000213793 2.348110e-02 3.335171849 1.669429e-01 8.694916e-01
ENSG00000180257 4.601416e-02 3.077122991 3.851676e-01 7.051491e-01
ENSG00000242779 -9.536143e-02 4.041796100 -1.050610e+00 3.089368e-01
ENSG00000170949 6.085996e-02 4.027401106 6.968147e-01 4.958475e-01
adj.P.Val B lfsr lfdr qvalue
ENSG00000225972 0.9988697 -4.7556698 0.9999658 0.9999532 0.9998023
ENSG00000225630 0.9988697 -4.6751535 0.9999632 0.9999565 0.9998240
ENSG00000237973 0.9988697 -3.4275354 0.9997019 0.9996975 0.9978198
ENSG00000248527 0.9988697 -4.3248057 0.9999284 0.9999224 0.9996087
ENSG00000198744 0.9988697 -4.6636194 0.9999722 0.9999652 0.9998687
ENSG00000228794 0.9988697 -4.7249782 0.9999826 0.9999686 0.9998850
ENSG00000223764 0.9988697 -4.7079583 0.9999812 0.9999640 0.9998627
ENSG00000187634 0.9988697 -4.6364752 0.9999661 0.9999582 0.9998324
ENSG00000188976 0.9988697 -4.9998747 0.9999892 0.9999834 0.9999356
ENSG00000187961 0.9988697 -4.2671574 0.9998938 0.9998885 0.9994126
ENSG00000187608 0.9988697 -4.5023427 0.9999458 0.9999383 0.9997128
ENSG00000188157 0.9988697 -4.3484778 0.9999361 0.9999311 0.9996671
ENSG00000131591 0.9988697 -4.4859956 0.9999419 0.9999358 0.9996995
ENSG00000078808 0.9988697 -4.6876281 0.9999756 0.9999716 0.9998974
ENSG00000176022 0.9988697 -4.3658485 0.9999430 0.9999384 0.9997134
ENSG00000160087 0.9988697 -5.0391015 0.9999926 0.9999868 0.9999425
ENSG00000131584 0.9988697 -4.5403688 0.9999631 0.9999587 0.9998353
ENSG00000169972 0.9988697 -4.8124697 0.9999863 0.9999792 0.9999242
ENSG00000127054 0.9988697 -4.7699176 0.9999837 0.9999803 0.9999270
ENSG00000224051 0.9988697 -4.9307464 0.9999890 0.9999801 0.9999265
ENSG00000107404 0.9988697 -4.7000345 0.9999694 0.9999639 0.9998617
ENSG00000162576 0.9988697 -4.4611026 0.9999477 0.9999424 0.9997377
ENSG00000175756 0.9988697 -4.8321211 0.9999867 0.9999834 0.9999356
ENSG00000221978 0.9988697 -4.7971345 0.9999680 0.9999605 0.9998452
ENSG00000224870 0.9988697 -4.8248243 0.9999826 0.9999757 0.9999132
ENSG00000242485 0.9988697 -4.9845914 0.9999897 0.9999844 0.9999378
ENSG00000160072 0.9988697 -4.8541580 0.9999876 0.9999808 0.9999283
ENSG00000197785 0.9988697 -4.6907996 0.9999742 0.9999691 0.9998874
ENSG00000160075 0.9988697 -5.0734495 0.9999929 0.9999881 0.9999442
ENSG00000228594 0.9988697 -4.5928390 0.9999605 0.9999534 0.9998034
ENSG00000197530 0.9988697 -4.2220070 0.9999209 0.9999160 0.9995753
ENSG00000189409 0.9988697 -4.7866573 0.9999746 0.9999672 0.9998784
ENSG00000248333 0.9988697 -4.8700517 0.9999871 0.9999778 0.9999194
ENSG00000189339 0.9988697 -4.8682518 0.9999866 0.9999824 0.9999329
ENSG00000008130 0.9988697 -4.9869846 0.9999913 0.9999867 0.9999423
ENSG00000078369 0.9988697 -5.0607060 0.9999949 0.9999902 0.9999455
ENSG00000162585 0.9988697 -4.5524658 0.9999595 0.9999542 0.9998081
ENSG00000157933 0.9988697 -4.9996716 0.9999901 0.9999858 0.9999409
ENSG00000157916 0.9988697 -4.5443906 0.9999778 0.9999753 0.9999116
ENSG00000157911 0.9988697 -4.8310285 0.9999827 0.9999735 0.9999051
ENSG00000157881 0.9988697 -4.8755390 0.9999898 0.9999831 0.9999348
ENSG00000157870 0.9988697 -4.4015611 0.9999306 0.9999251 0.9996241
ENSG00000158109 0.9988697 -5.0752671 0.9999925 0.9999874 0.9999435
ENSG00000116213 0.9988697 -4.8935121 0.9999914 0.9999833 0.9999352
ENSG00000227372 0.9988697 -4.6773456 0.9999782 0.9999735 0.9999051
ENSG00000130764 0.9988697 -3.8083848 0.9998855 0.9998829 0.9993784
ENSG00000116198 0.9988697 -4.8679113 0.9999859 0.9999807 0.9999281
ENSG00000198912 0.9988697 -4.9416971 0.9999914 0.9999842 0.9999375
ENSG00000131697 0.9988697 -4.7710856 0.9999857 0.9999784 0.9999213
ENSG00000116251 0.9988697 -5.0099443 0.9999884 0.9999832 0.9999349
ENSG00000116237 0.9988697 -5.0120439 0.9999913 0.9999873 0.9999434
ENSG00000158292 0.9988697 -4.5836408 0.9999603 0.9999533 0.9998028
ENSG00000097021 0.9988697 -4.9866317 0.9999838 0.9999682 0.9998830
ENSG00000215788 0.9988697 -4.6685682 0.9999693 0.9999555 0.9998170
ENSG00000171680 0.9988697 -3.9861524 0.9998716 0.9998660 0.9992556
ENSG00000162408 0.9988697 -4.7879552 0.9999800 0.9999728 0.9999025
ENSG00000204859 0.9988697 -4.8040514 0.9999855 0.9999752 0.9999113
ENSG00000162413 0.9988697 -4.8648307 0.9999853 0.9999814 0.9999302
ENSG00000116273 0.9988697 -4.9959511 0.9999905 0.9999820 0.9999319
ENSG00000041988 0.9988697 -4.7812317 0.9999845 0.9999767 0.9999162
ENSG00000007923 0.9988697 -4.9323407 0.9999854 0.9999789 0.9999233
ENSG00000171735 0.9988697 -4.6699700 0.9999663 0.9999589 0.9998359
ENSG00000049245 0.9988697 -5.0168864 0.9999917 0.9999878 0.9999439
ENSG00000116288 0.9988697 -5.0734283 0.9999928 0.9999880 0.9999441
ENSG00000116285 0.9988697 -4.5923458 0.9999496 0.9999403 0.9997230
ENSG00000142599 0.9988697 -4.4858616 0.9999519 0.9999469 0.9997634
ENSG00000074800 0.9988697 -4.6131120 0.9999755 0.9999721 0.9998997
ENSG00000142583 0.9988697 -4.6419235 0.9999650 0.9999524 0.9997973
ENSG00000180758 0.9988697 -4.3886672 0.9999155 0.9999109 0.9995442
ENSG00000228526 0.9988697 -4.7832151 0.9999786 0.9999620 0.9998528
ENSG00000049239 0.9988697 -4.9922368 0.9999841 0.9999765 0.9999157
ENSG00000171621 0.9988697 -5.0738506 0.9999889 0.9999808 0.9999286
ENSG00000171612 0.9988697 -4.7002333 0.9999745 0.9999656 0.9998703
ENSG00000188807 0.9988697 -4.2439117 0.9998913 0.9998869 0.9993999
ENSG00000171608 0.9988697 -4.7890797 0.9999797 0.9999704 0.9998927
ENSG00000171603 0.9988697 -5.0301047 0.9999895 0.9999842 0.9999374
ENSG00000178585 0.9988697 -4.7075975 0.9999776 0.9999723 0.9999006
ENSG00000162441 0.9988697 -4.7826072 0.9999812 0.9999753 0.9999117
ENSG00000173614 0.9988697 -4.1595827 0.9998897 0.9998852 0.9993921
ENSG00000130939 0.9988697 -5.0766280 0.9999927 0.9999859 0.9999411
ENSG00000054523 0.9988697 -4.8458515 0.9999867 0.9999835 0.9999356
ENSG00000142657 0.9988697 -4.3169658 0.9999623 0.9999597 0.9998410
ENSG00000160049 0.9988697 -5.0334546 0.9999938 0.9999887 0.9999448
ENSG00000142655 0.9988697 -4.6004190 0.9999718 0.9999674 0.9998789
ENSG00000120948 0.9988697 -4.2477560 0.9999572 0.9999547 0.9998120
ENSG00000116649 0.9988697 -5.0911109 0.9999900 0.9999816 0.9999306
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[ reached 'max' / getOption("max.print") -- omitted 448 rows ]
[[12]]
logFC AveExpr t P.Value
ENSG00000225972 7.184465e-01 3.439734306 2.8001391126 1.251162e-02
ENSG00000225630 2.556881e-01 5.259763521 1.2223723556 2.386482e-01
ENSG00000237973 2.828234e-01 5.765427761 1.6259618988 1.227989e-01
ENSG00000248527 3.654397e-01 7.291375670 1.9013020942 7.477469e-02
ENSG00000198744 -5.229600e-01 1.692163426 -0.8593654016 4.023849e-01
ENSG00000228794 -2.685819e-01 2.429990051 -1.0338345789 3.160481e-01
ENSG00000223764 2.208487e-01 1.604113326 0.7712055339 4.514291e-01
ENSG00000187634 6.335468e-03 2.774428967 0.0300042754 9.764216e-01
ENSG00000188976 1.274186e-01 5.602324306 1.1194965135 2.788736e-01
ENSG00000187961 -3.543970e-02 3.205023751 -0.1666425949 8.696652e-01
ENSG00000187608 4.903646e-01 3.515541854 2.1357284599 4.792241e-02
ENSG00000188157 3.308109e-01 8.438003633 2.0758993695 5.377924e-02
ENSG00000131591 4.499033e-02 2.698849622 0.2011298545 8.430421e-01
ENSG00000078808 8.432083e-02 8.404430472 0.7227303209 4.799100e-01
ENSG00000176022 4.939323e-02 5.315643527 0.3071623660 7.625415e-01
ENSG00000160087 -7.974216e-02 5.574117382 -0.7984740044 4.358763e-01
ENSG00000131584 -5.567335e-02 5.338644789 -0.4008365059 6.936551e-01
ENSG00000169972 6.293699e-02 3.649507997 0.4091599618 6.876555e-01
ENSG00000127054 9.054267e-02 5.813712471 0.9521302323 3.546965e-01
ENSG00000224051 -2.071206e-01 4.433660580 -1.4275433201 1.719741e-01
ENSG00000107404 -1.823149e-01 5.541314411 -1.1145099983 2.809448e-01
ENSG00000162576 2.803710e-01 5.643016003 1.5406326361 1.422607e-01
ENSG00000175756 1.317532e-01 5.949578662 1.5268767773 1.456289e-01
ENSG00000221978 -7.399060e-02 6.117640537 -0.3712595649 7.151423e-01
ENSG00000224870 2.265221e-01 4.193043833 1.3685521289 1.893858e-01
ENSG00000242485 -1.280256e-02 5.474356677 -0.1099774378 9.137465e-01
ENSG00000160072 5.058305e-02 4.020742816 0.3769759928 7.109694e-01
ENSG00000197785 2.044551e-01 4.667370310 1.4273714962 1.720229e-01
ENSG00000160075 -8.361158e-02 6.698987873 -0.9523936334 3.545669e-01
ENSG00000228594 -1.139327e-01 3.231082811 -0.4824707446 6.357752e-01
ENSG00000197530 4.450160e-01 6.011139637 2.9227231328 9.676061e-03
ENSG00000189409 2.040662e-01 4.590321361 0.9020854080 3.799209e-01
ENSG00000248333 2.270171e-01 3.904469734 1.3366989051 1.993624e-01
ENSG00000189339 1.413689e-01 5.329578573 1.3445282597 1.968721e-01
ENSG00000008130 1.088553e-01 5.472226379 1.1385243216 2.710744e-01
ENSG00000078369 -4.362756e-02 8.804321589 -0.5870694216 5.650609e-01
ENSG00000162585 3.055515e-01 4.754428869 1.8767886959 7.824737e-02
ENSG00000157933 -1.848720e-02 6.562145698 -0.1939462501 8.485726e-01
ENSG00000157916 -5.716210e-02 7.002652621 -0.7174188555 4.830947e-01
ENSG00000157911 3.941156e-01 3.826112744 1.9737660941 6.529972e-02
ENSG00000157881 -9.662778e-02 4.048444809 -0.7434438056 4.676107e-01
ENSG00000157870 2.428724e-01 3.237377913 1.2051351790 2.450589e-01
ENSG00000158109 -3.366371e-02 6.732297019 -0.3663029637 7.187681e-01
ENSG00000116213 -1.738557e-01 4.048572327 -1.3394446752 1.984862e-01
ENSG00000227372 2.488627e-01 3.931432132 1.8972027486 7.534569e-02
ENSG00000130764 1.045934e-01 5.498222486 0.9471229030 3.571673e-01
ENSG00000116198 -1.298676e-02 4.799410870 -0.0996167987 9.218423e-01
ENSG00000198912 -9.647284e-03 4.517142545 -0.0729526323 9.427161e-01
ENSG00000131697 1.417354e-01 3.217207294 0.8582032120 4.030080e-01
ENSG00000116251 -1.031630e-01 6.491595851 -0.9393659784 3.610181e-01
ENSG00000116237 -7.959071e-03 7.144503644 -0.0937558268 9.264260e-01
ENSG00000158292 -1.961635e-01 2.850382649 -0.9229606187 3.692556e-01
ENSG00000097021 -5.519796e-01 4.963811963 -2.2234430020 4.038836e-02
ENSG00000215788 2.417255e-02 2.224832337 0.0725830818 9.430057e-01
ENSG00000171680 4.248122e-01 4.816276482 1.9591728316 6.711628e-02
ENSG00000162408 -1.584423e-01 3.954485497 -0.9465439695 3.574537e-01
ENSG00000204859 -1.291178e-02 3.319571732 -0.0659902573 9.481742e-01
ENSG00000162413 -5.176009e-03 6.835844141 -0.0514765984 9.595604e-01
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ENSG00000090924 1.715407e-01 5.472189297 1.2724149748 2.207692e-01
ENSG00000105193 2.448785e-02 8.011360970 0.2175803163 8.304094e-01
ENSG00000196235 2.075770e-01 7.297426852 2.2872879193 3.561056e-02
ENSG00000105197 6.553868e-02 5.383776015 0.7596798639 4.581048e-01
ENSG00000176396 -1.300963e-01 4.054007587 -0.8844708462 3.890797e-01
ENSG00000105204 2.365212e-01 4.157321077 1.3512255153 1.947617e-01
ENSG00000105202 -4.625581e-03 5.006808973 -0.0421350658 9.668939e-01
ENSG00000013275 -6.162913e-02 6.388777941 -0.6828009822 5.041555e-01
ENSG00000128000 -2.356065e-01 2.864242766 -0.9627262708 3.495071e-01
ENSG00000197782 -5.114658e-02 2.910232088 -0.2491016876 8.063396e-01
ENSG00000130758 2.781292e-01 4.132403833 1.7127131311 1.053878e-01
ENSG00000105221 1.609545e-01 6.233160376 1.7947785396 9.092770e-02
ENSG00000160392 3.060191e-01 3.576580916 1.6973200726 1.083120e-01
ENSG00000105223 1.491685e-01 9.823655984 1.2309706827 2.354990e-01
ENSG00000105227 1.707475e-02 2.380604594 0.0718427599 9.435859e-01
ENSG00000197019 -2.884095e-01 5.043829216 -2.5069667019 2.290605e-02
ENSG00000167565 1.046210e-01 4.710856228 0.9511458509 3.551813e-01
ENSG00000090013 -1.510024e-01 4.251663177 -1.0001816970 3.315870e-01
ENSG00000160410 1.044477e-01 4.774910152 0.6224245544 5.421231e-01
ENSG00000090006 1.190096e-01 5.866256191 0.7849904256 4.435242e-01
ENSG00000105245 -1.896426e-01 5.218498010 -1.5475059838 1.406023e-01
ENSG00000123815 2.863405e-01 3.654175556 1.7174927250 1.044939e-01
ENSG00000086544 -4.629013e-01 5.243565476 -3.6000755660 2.282374e-03
ENSG00000188493 3.008534e-01 2.503604906 0.7363353457 4.718100e-01
ENSG00000077312 9.534546e-02 3.755007048 0.6784719747 5.068260e-01
ENSG00000167601 -1.445521e-01 7.008455671 -1.5054031888 1.510199e-01
ENSG00000105323 2.106897e-01 6.965390046 2.2375456586 3.928451e-02
ENSG00000105329 -1.619233e-01 7.473825597 -0.8430042992 4.112146e-01
ENSG00000142039 1.770347e-01 4.317937467 0.9689883413 3.464651e-01
ENSG00000077348 2.435742e-02 2.932837657 0.1188035198 9.068575e-01
ENSG00000105341 8.602616e-02 4.759308037 0.7055514727 4.902553e-01
ENSG00000105372 -8.968473e-02 9.740957245 -0.9096672585 3.760236e-01
ENSG00000076928 2.071613e-01 5.629632147 1.8305097052 8.519580e-02
ENSG00000105404 3.625795e-01 5.798744407 2.9999556200 8.221486e-03
ENSG00000105732 3.478097e-02 3.844511657 0.2155218027 8.319876e-01
ENSG00000160570 -7.187538e-02 4.689229177 -0.5551576675 5.861944e-01
ENSG00000167625 1.438951e-01 4.360927097 0.9266583700 3.673878e-01
ENSG00000105723 -1.429091e-01 5.219372236 -1.1200311742 2.786522e-01
ENSG00000105722 2.618729e-02 4.762170184 0.1803731247 8.590444e-01
ENSG00000079432 2.963775e-01 5.997744369 2.5045047851 2.302104e-02
ENSG00000105429 3.504089e-01 6.747840921 2.1740325159 4.448602e-02
ENSG00000176531 4.380684e-01 3.052308073 2.3021584788 3.457590e-02
ENSG00000105755 3.119504e-02 4.616638217 0.2871838486 7.775273e-01
ENSG00000073050 9.070548e-02 3.610658799 0.6060333378 5.526941e-01
ENSG00000167378 -1.525011e-01 4.985663450 -0.9225183534 3.694795e-01
ENSG00000124444 -7.124036e-02 3.568820639 -0.4786307916 6.384477e-01
ENSG00000131116 1.224045e-01 4.033710206 0.6573585852 5.199661e-01
ENSG00000011422 -6.560013e-01 5.122736652 -2.2980174052 3.486117e-02
ENSG00000105771 -5.076967e-02 5.116711790 -0.4635422379 6.489986e-01
ENSG00000167637 1.975305e-01 2.592650824 1.0046193226 3.295075e-01
ENSG00000176222 1.011085e-01 2.219045231 0.4994806134 6.239992e-01
ENSG00000124459 -1.939008e-01 4.346980811 -1.2890961026 2.150479e-01
ENSG00000159885 -4.694958e-01 2.185741583 -1.9760003932 6.502553e-02
ENSG00000267680 -2.654646e-01 2.660724199 -0.9535062326 3.540197e-01
ENSG00000256294 -2.715953e-01 2.692183696 -1.1462329573 2.679617e-01
ENSG00000263002 -3.873571e-01 3.408531106 -2.2799682280 3.613037e-02
ENSG00000167380 1.503894e-01 4.441687498 0.8990651745 3.814809e-01
ENSG00000131115 -1.866948e-01 2.992248604 -1.2286778966 2.363356e-01
ENSG00000062370 -3.880362e-01 2.401433710 -1.6939639744 1.089588e-01
ENSG00000278318 -2.506080e-01 0.979126751 -1.7706955054 9.497958e-02
ENSG00000167384 -9.006840e-02 2.427775316 -0.3943964726 6.983116e-01
ENSG00000073008 -5.029081e-02 6.628771200 -0.5569516504 5.849958e-01
ENSG00000279095 -2.470003e-01 2.586428111 -1.1454663153 2.682700e-01
ENSG00000069399 -1.481839e-01 3.339772580 -0.5746154619 5.732608e-01
ENSG00000130202 1.356638e-01 6.894434617 1.0576483191 3.053723e-01
ENSG00000130204 9.338990e-02 4.659605433 0.7087890555 4.882956e-01
ENSG00000130203 -1.914357e-01 3.143686136 -0.5767583660 5.718455e-01
ENSG00000104853 7.249119e-02 6.993213285 0.7782435827 4.473823e-01
ENSG00000104856 -7.044167e-01 2.766145266 -2.2544868357 3.799547e-02
ENSG00000104859 1.725603e-01 4.072995944 1.3294744652 2.016825e-01
ENSG00000142252 1.491490e-01 3.467358598 1.0833098547 2.941633e-01
ENSG00000007047 8.306686e-02 5.363544183 0.5650292456 5.796143e-01
ENSG00000104866 2.687370e-02 4.825681576 0.1781990281 8.607243e-01
ENSG00000007255 6.007484e-01 2.891837829 1.8219627533 8.653710e-02
ENSG00000189114 1.008487e-01 4.089487234 0.6799989819 5.058831e-01
ENSG00000104884 2.062311e-01 4.719206848 1.2008862803 2.466593e-01
ENSG00000104881 -1.926766e-01 4.899760551 -0.9722700924 3.448783e-01
ENSG00000012061 -2.320672e-01 5.266235979 -1.5511467415 1.397305e-01
ENSG00000125744 -2.926208e-01 3.270864718 -1.9785807836 6.471016e-02
ENSG00000125753 -9.767876e-02 6.542329712 -0.7027040285 4.919826e-01
ENSG00000125741 8.197449e-02 5.027972167 0.7260433325 4.779300e-01
ENSG00000125746 1.701805e-01 4.067216554 1.3970081967 1.808167e-01
ENSG00000125743 1.054299e-01 6.918742564 1.0695521391 3.001347e-01
ENSG00000011478 -1.531718e-02 5.400119293 -0.1475896533 8.844453e-01
ENSG00000177051 -2.647510e-01 2.991101820 -1.2616775322 2.245145e-01
ENSG00000177045 6.666246e-01 3.224570072 1.9242648045 7.164703e-02
ENSG00000104936 1.578293e-01 5.370633599 1.1274872635 2.755782e-01
ENSG00000185800 1.939843e-01 5.023702953 1.4795039380 1.577419e-01
ENSG00000125755 1.303347e-01 6.003761254 1.0548239604 3.066246e-01
ENSG00000170604 5.625143e-01 4.613920159 2.8964919228 1.022484e-02
ENSG00000176182 1.397020e-01 3.618458128 0.7932275989 4.388422e-01
ENSG00000188624 5.384676e-01 2.451040156 2.4245236421 2.706821e-02
ENSG00000204866 1.766898e-01 6.246041858 1.5717899740 1.348727e-01
ENSG00000124440 -2.498487e-01 3.202673522 -0.9742899149 3.439042e-01
ENSG00000011485 -8.948372e-02 5.531932188 -0.6374488494 5.325311e-01
ENSG00000169515 2.385751e-01 6.217376588 1.8572801615 8.111270e-02
ENSG00000182013 5.928625e-02 4.098389913 0.3558781559 7.264164e-01
ENSG00000160014 6.059540e-02 6.669841601 0.7103036625 4.873804e-01
ENSG00000197380 -1.680439e-01 1.871194780 -0.4819041126 6.361692e-01
ENSG00000105287 4.992164e-02 3.771143271 0.2664977849 7.931395e-01
ENSG00000090372 1.277426e-01 5.820892491 1.1516772783 2.657795e-01
ENSG00000181027 2.282607e-01 4.641894417 1.4029422659 1.790700e-01
ENSG00000105281 5.881855e-02 7.857433925 0.5905440883 5.627841e-01
ENSG00000042753 -4.752872e-03 7.434923049 -0.0560053742 9.560064e-01
ENSG00000160007 1.711387e-01 6.288965066 1.7853687658 9.249245e-02
ENSG00000130751 -1.041946e-01 1.595824281 -0.2674030930 7.924543e-01
ENSG00000130748 -8.183583e-02 3.344774900 -0.3301627172 7.454092e-01
ENSG00000130749 2.163079e-01 4.763281284 1.1804637465 2.544631e-01
ENSG00000142230 1.193979e-01 6.729612621 1.2875600113 2.155698e-01
ENSG00000105327 8.614482e-02 3.801955028 0.3933599843 6.990622e-01
ENSG00000105321 1.375359e-01 4.678268012 1.0002328978 3.315629e-01
ENSG00000257704 1.079645e-01 3.298874371 0.4768952846 6.396573e-01
ENSG00000134815 -7.795007e-02 3.693557527 -0.4452985767 6.618591e-01
ENSG00000105419 7.821649e-02 4.933703137 0.5858237173 5.658783e-01
ENSG00000105402 1.620934e-01 5.408501710 1.2210511426 2.391349e-01
ENSG00000063169 2.888101e-01 3.327719514 1.2945091291 2.132167e-01
ENSG00000024422 3.808083e-02 8.032530303 0.2655013050 7.938940e-01
ENSG00000105373 -4.256394e-02 5.891610812 -0.4215713174 6.787491e-01
ENSG00000178980 -6.611415e-02 7.035800120 -0.6750951707 5.089147e-01
ENSG00000105499 -4.997628e-01 1.927616636 -1.3909467742 1.826152e-01
ENSG00000105486 5.178734e-01 3.002420359 2.1695975526 4.487196e-02
ENSG00000185453 1.662475e-01 2.708174192 0.8019643389 4.339102e-01
ENSG00000105483 -2.810039e-02 3.977034748 -0.2180807930 8.300258e-01
ENSG00000142227 -3.600424e-01 5.980823546 -2.5913078620 1.928012e-02
ENSG00000105438 -3.581386e-02 8.088896974 -0.4005864621 6.938356e-01
ENSG00000105464 -1.146151e-02 1.667123160 -0.0420728397 9.669428e-01
ENSG00000105447 1.501810e-01 4.746674892 1.2717062535 2.210148e-01
ENSG00000105443 8.292142e-03 5.536496313 0.0526819141 9.586144e-01
ENSG00000063177 -1.401835e-02 8.145714965 -0.1323201655 8.963225e-01
ENSG00000063176 4.052852e-01 4.421560402 2.6316796053 1.774463e-02
ENSG00000105552 -6.529473e-03 3.944928364 -0.0480206572 9.622731e-01
ENSG00000087076 3.437643e-01 4.966516712 1.8021309202 8.972129e-02
ENSG00000087074 9.128512e-02 6.783914463 0.6463904241 5.268674e-01
ENSG00000104805 2.641010e-01 7.906149214 2.1604833728 4.567478e-02
ENSG00000087088 4.018566e-01 6.436261320 3.0272931359 7.759584e-03
ENSG00000087086 -6.791740e-02 12.058941043 -0.5727145479 5.745178e-01
ENSG00000104812 2.672014e-02 5.554919935 0.2798586152 7.830450e-01
ENSG00000183207 2.875467e-01 5.649854617 2.4982004637 2.331801e-02
ENSG00000104852 2.224702e-01 6.570625396 1.5363974644 1.432907e-01
ENSG00000104863 2.277814e-01 2.860985462 1.1139952763 2.811592e-01
ENSG00000177380 4.676903e-01 2.904772987 2.1191607351 4.948289e-02
ENSG00000130529 2.306270e-01 3.954710118 1.0848888579 2.934835e-01
ENSG00000074219 1.507523e-01 4.784127661 1.1019861142 2.861970e-01
ENSG00000104872 2.613332e-02 5.215611657 0.2045203064 8.404347e-01
ENSG00000161618 2.319206e-01 4.073193668 1.1072261966 2.839907e-01
ENSG00000142541 -1.915731e-02 8.798495572 -0.1984053625 8.451386e-01
ENSG00000142534 1.266387e-02 9.214561396 0.1076294283 9.155804e-01
ENSG00000104870 9.263790e-02 5.305007871 0.6016043849 5.555693e-01
ENSG00000142552 2.243315e-01 6.302870351 1.7322587227 1.017736e-01
ENSG00000142546 -7.712513e-02 5.367518939 -0.7088273702 4.882725e-01
ENSG00000126464 2.449246e-01 4.885551572 1.5884029428 1.310675e-01
ENSG00000126458 1.213896e-01 6.339176461 1.3568604418 1.930001e-01
ENSG00000126461 3.119709e-01 6.133402435 1.7744030239 9.434569e-02
ENSG00000126456 -4.148368e-02 4.969957329 -0.3292476428 7.460882e-01
ENSG00000126453 3.254994e-01 2.842914353 1.5525405126 1.393979e-01
ENSG00000126457 2.024895e-01 6.324574391 1.9526993009 6.793655e-02
ENSG00000196961 -1.711452e-03 6.862993870 -0.0131837141 9.896385e-01
ENSG00000010361 -8.066968e-02 2.746988061 -0.3023642626 7.661319e-01
ENSG00000104973 2.196117e-01 4.644685303 1.2662763170 2.229043e-01
ENSG00000104960 -1.368297e-01 5.126602512 -1.1518172889 2.657235e-01
ENSG00000039650 8.751353e-02 4.318555072 0.6100494758 5.500938e-01
ENSG00000204673 9.174503e-03 6.615684364 0.0706565027 9.445158e-01
ENSG00000104946 1.307383e-01 5.142717077 0.9604039715 3.506399e-01
ENSG00000213024 1.995840e-01 5.178261322 2.1265035006 4.878563e-02
ENSG00000169136 7.572827e-02 3.340949893 0.4455191823 6.617029e-01
ENSG00000105053 -2.539656e-01 4.303539969 -1.5614722482 1.372826e-01
ENSG00000142528 -3.490665e-01 3.054946621 -1.9556175047 6.756567e-02
ENSG00000131408 4.872805e-02 5.748430698 0.3607373775 7.228476e-01
ENSG00000062822 2.085180e-01 3.000646471 0.9445441059 3.584443e-01
ENSG00000161671 1.177293e-01 6.833897974 1.0062823076 3.287307e-01
ENSG00000161677 6.615302e-02 4.863914065 0.4616201275 6.503483e-01
ENSG00000167747 3.262302e-01 4.345002868 2.1978468355 4.246543e-02
ENSG00000142544 -3.897445e-01 2.311663930 -1.1286290596 2.751097e-01
ENSG00000105379 4.859891e-01 3.407808788 2.5050506877 2.299550e-02
ENSG00000105497 -5.838850e-02 3.196139230 -0.3740864830 7.130775e-01
ENSG00000182310 -2.593904e-01 2.485832085 -1.2287513513 2.363087e-01
ENSG00000198093 5.671649e-03 2.053797321 0.0238733526 9.812384e-01
ENSG00000176024 2.980020e-01 2.592669391 1.5071694063 1.505703e-01
ENSG00000256683 -5.969336e-02 2.455207926 -0.2890043651 7.761579e-01
ENSG00000197619 -5.004519e-01 2.482955439 -1.7566795810 9.740971e-02
ENSG00000142556 -2.520392e-01 3.382865635 -1.5004707955 1.522814e-01
ENSG00000256087 2.120414e-02 4.021520586 0.1043190301 9.181668e-01
ENSG00000197608 -1.221912e-01 4.006239358 -0.7686735287 4.528904e-01
ENSG00000204611 -3.422084e-02 3.850670783 -0.2205059071 8.281676e-01
ENSG00000105568 -5.877229e-02 7.439852649 -0.5115358990 6.157162e-01
ENSG00000196214 1.235480e-02 3.198031676 0.0775892201 9.390828e-01
ENSG00000198464 1.429722e-01 3.424444091 0.6752681163 5.088076e-01
ENSG00000167554 -1.154861e-01 2.135684048 -0.5110118475 6.160751e-01
ENSG00000221923 -5.245455e-02 2.348356875 -0.2626557304 7.960495e-01
ENSG00000167555 7.763522e-02 2.377642494 0.4369497287 6.677813e-01
ENSG00000167766 -1.817830e-01 3.897495780 -1.2011242386 2.465694e-01
ENSG00000213020 -2.996482e-01 2.480214607 -1.6567896748 1.163485e-01
ENSG00000189190 -2.221219e-01 1.940346670 -0.7115353091 4.866370e-01
ENSG00000198538 -4.692054e-01 3.912907898 -3.1100459100 6.510659e-03
ENSG00000204604 -4.543573e-01 4.547968222 -2.5962737522 1.908463e-02
ENSG00000213793 3.078365e-03 3.335171849 0.0147161016 9.884342e-01
ENSG00000180257 4.690308e-02 3.077122991 0.2714432135 7.893986e-01
ENSG00000242779 1.413781e-01 4.041796100 0.9173995881 3.720768e-01
ENSG00000170949 -1.351516e-01 4.027401106 -1.1524697923 2.654629e-01
adj.P.Val B lfsr lfdr qvalue
ENSG00000225972 0.26566844 -2.811634180 0.34294207 0.23286468 0.16590909
ENSG00000225630 0.59716344 -5.262658421 0.47127226 0.30863104 0.22149938
ENSG00000237973 0.46970095 -4.945008947 0.37416772 0.26405722 0.18752946
ENSG00000248527 0.39269205 -4.543288323 0.35164260 0.24661995 0.17570223
ENSG00000198744 0.72643952 -4.685566237 0.60754260 0.32503398 0.23515046
ENSG00000228794 0.66332047 -4.952216675 0.52521097 0.32223398 0.23270062
ENSG00000223764 0.75477320 -5.152258636 0.57004720 0.33309297 0.24263121
ENSG00000187634 0.99258781 -5.648976678 0.67912164 0.36783559 0.27069201
ENSG00000188976 0.63417333 -5.488506024 0.44660151 0.33359137 0.24313161
ENSG00000187961 0.95807156 -5.589417820 0.65651047 0.36599756 0.26953670
ENSG00000187608 0.34342418 -3.807810638 0.36502261 0.24885781 0.17737194
ENSG00000188157 0.35588244 -4.237349097 0.28519858 0.21024153 0.15008883
ENSG00000131591 0.94793460 -5.407838491 0.64990896 0.36171334 0.26644988
ENSG00000078808 0.77097034 -5.913319881 0.55401735 0.39319322 0.28596600
ENSG00000176022 0.91353877 -6.014000684 0.63810056 0.38855570 0.28357517
ENSG00000160087 0.74588038 -5.784007201 0.54373792 0.40470807 0.29138902
ENSG00000131584 0.88065557 -6.001352490 0.62399704 0.40156934 0.29008618
ENSG00000169972 0.87758815 -5.610139098 0.61825566 0.38840055 0.28349399
ENSG00000127054 0.69358924 -5.674061660 0.50284155 0.38485786 0.28169366
ENSG00000224051 0.52573776 -4.963655615 0.38539786 0.27950201 0.19996040
ENSG00000107404 0.63624467 -5.501373217 0.46527915 0.31894816 0.22984869
ENSG00000162576 0.49388180 -4.960638962 0.39658794 0.27536714 0.19685839
ENSG00000175756 0.49806960 -5.039924718 0.33093710 0.26930468 0.19184965
ENSG00000221978 0.89271021 -6.109805150 0.62178555 0.36563982 0.26933644
ENSG00000224870 0.55039120 -4.961768533 0.41420155 0.29000994 0.20653834
ENSG00000242485 0.97423037 -6.074375302 0.69996496 0.44317955 0.30639655
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ENSG00000105053 0.48940210 -4.742408135 0.37442586 0.26713970 0.19019303
ENSG00000142528 0.38208466 -4.025112226 0.32777884 0.23436236 0.16690361
ENSG00000131408 0.89696978 -6.038733407 0.63439855 0.40798304 0.29279247
ENSG00000062822 0.69525958 -5.102705513 0.52390635 0.32901422 0.23890686
ENSG00000161671 0.67252627 -5.695898381 0.47757438 0.35051530 0.25758281
ENSG00000161677 0.86103521 -5.853096105 0.60906603 0.39332732 0.28603355
ENSG00000167747 0.33356118 -3.811520004 0.25192904 0.18947902 0.13533508
ENSG00000142544 0.62972388 -4.885230293 0.54272883 0.31914292 0.23005191
ENSG00000105379 0.29093102 -3.208998839 0.28611073 0.20499914 0.14580341
ENSG00000105497 0.89182273 -5.523261212 0.62524433 0.38871869 0.28362929
ENSG00000182310 0.59450947 -4.775945227 0.47135993 0.30824930 0.22123660
ENSG00000198093 0.99390605 -5.329819735 0.67610436 0.35928100 0.26458424
ENSG00000176024 0.50270018 -4.478829843 0.41600453 0.28275108 0.20208796
ENSG00000256683 0.91985493 -5.375002950 0.63615142 0.36558108 0.26932214
ENSG00000197619 0.43065565 -4.224277921 0.44933393 0.28707062 0.20462852
ENSG00000142556 0.50392263 -4.663895747 0.39089152 0.27562084 0.19699100
ENSG00000256087 0.97547951 -5.824009838 0.66818898 0.37047872 0.27243441
ENSG00000197608 0.75580548 -5.565835441 0.53932660 0.35677428 0.26266128
ENSG00000204611 0.94169406 -5.753170738 0.65711710 0.39607551 0.28739749
ENSG00000105568 0.84458859 -6.067375840 0.60919091 0.42028568 0.29796395
ENSG00000196214 0.98309232 -5.596423211 0.68372808 0.39601445 0.28734343
ENSG00000198464 0.79114363 -5.346940716 0.56810329 0.34717854 0.25499195
ENSG00000167554 0.84485914 -5.240671068 0.59868863 0.35190448 0.25890285
ENSG00000221923 0.92882009 -5.476911285 0.64123316 0.36898440 0.27141653
ENSG00000167555 0.86964743 -5.650781868 0.60991517 0.37224501 0.27368479
ENSG00000167766 0.60503624 -5.122101686 0.43966762 0.30966547 0.22256510
ENSG00000213020 0.45919634 -4.342213705 0.37603105 0.26356540 0.18709160
ENSG00000189190 0.77652304 -5.038778646 0.58320957 0.33345864 0.24300676
ENSG00000198538 0.22842433 -2.207392926 0.16940152 0.12945808 0.09506982
ENSG00000204604 0.28099750 -3.143221399 0.24938336 0.18316453 0.13105479
ENSG00000213793 0.99618400 -5.577384768 0.68194692 0.36862547 0.27118943
ENSG00000180257 0.92601320 -5.499323929 0.64289078 0.38213236 0.27997040
ENSG00000242779 0.70563184 -5.255754767 0.50492236 0.34325870 0.25159449
ENSG00000170949 0.62036937 -5.184497036 0.43781949 0.32579398 0.23586388
svalue
ENSG00000225972 0.23383858
ENSG00000225630 0.31965143
ENSG00000237973 0.25472719
ENSG00000248527 0.23983742
ENSG00000198744 0.40986842
ENSG00000228794 0.35498840
ENSG00000223764 0.38470169
ENSG00000187634 0.45779705
ENSG00000188976 0.30302568
ENSG00000187961 0.44346523
ENSG00000187608 0.24906596
ENSG00000188157 0.19736340
ENSG00000131591 0.43904836
ENSG00000078808 0.37444584
ENSG00000176022 0.43087243
ENSG00000160087 0.36747577
ENSG00000131584 0.42111288
ENSG00000169972 0.41745440
ENSG00000127054 0.34031347
ENSG00000224051 0.26256076
ENSG00000107404 0.31523797
ENSG00000162576 0.26927183
ENSG00000175756 0.22578160
ENSG00000221978 0.41994688
ENSG00000224870 0.28124252
ENSG00000242485 0.46741231
ENSG00000160072 0.42562621
ENSG00000197785 0.26139838
ENSG00000160075 0.34552348
ENSG00000228594 0.40766833
ENSG00000197530 0.13208750
ENSG00000189409 0.36022606
ENSG00000248333 0.28804016
ENSG00000189339 0.26085634
ENSG00000008130 0.30256191
ENSG00000078369 0.43247370
ENSG00000162585 0.21873388
ENSG00000157933 0.46816201
ENSG00000157916 0.40119743
ENSG00000157911 0.23882845
ENSG00000157881 0.36820525
ENSG00000157870 0.31850518
ENSG00000158109 0.45058184
ENSG00000116213 0.26805217
ENSG00000227372 0.18972963
ENSG00000130764 0.33613239
ENSG00000116198 0.46449963
ENSG00000198912 0.46624343
ENSG00000131697 0.35194095
ENSG00000116251 0.33759383
ENSG00000116237 0.47723079
ENSG00000158292 0.35462431
ENSG00000097021 0.25588081
ENSG00000215788 0.44751223
ENSG00000171680 0.25305215
ENSG00000162408 0.34025023
ENSG00000204859 0.45603111
ENSG00000162413 0.47532443
ENSG00000116273 0.06248463
ENSG00000041988 0.43419082
ENSG00000007923 0.21408983
ENSG00000171735 0.25180423
ENSG00000049245 0.26164802
ENSG00000116288 0.13485439
ENSG00000116285 0.23190775
ENSG00000142599 0.32562713
ENSG00000074800 0.44573088
ENSG00000142583 0.34408921
ENSG00000180758 0.37664720
ENSG00000228526 0.43880684
ENSG00000049239 0.42088485
ENSG00000171621 0.28244238
ENSG00000171612 0.21893558
ENSG00000188807 0.40395560
ENSG00000171608 0.15781654
ENSG00000171603 0.40730038
ENSG00000178585 0.29169179
ENSG00000162441 0.37081850
ENSG00000173614 0.33119119
ENSG00000130939 0.45988484
ENSG00000054523 0.40669614
ENSG00000142657 0.47643178
ENSG00000160049 0.38212000
ENSG00000142655 0.33743541
ENSG00000120948 0.47362803
ENSG00000116649 0.33296560
ENSG00000171824 0.40540679
ENSG00000198793 0.04759237
ENSG00000171819 0.45081682
ENSG00000120942 0.45593798
ENSG00000116661 0.42326075
ENSG00000132879 0.44649174
ENSG00000116670 0.30765750
ENSG00000177674 0.40979009
ENSG00000177000 0.17715210
ENSG00000011021 0.26862140
ENSG00000116685 0.37584727
ENSG00000083444 0.33907956
ENSG00000116688 0.21003369
ENSG00000116691 0.35337803
ENSG00000048707 0.21307055
ENSG00000162496 0.25829619
ENSG00000162494 0.27484811
ENSG00000162493 0.05971603
ENSG00000116731 0.47574109
ENSG00000189337 0.41763299
ENSG00000171729 0.16431770
ENSG00000142634 0.25774754
ENSG00000132906 0.40945044
ENSG00000116138 0.18565017
ENSG00000197312 0.13116538
ENSG00000116786 0.41898259
ENSG00000162458 0.27662801
ENSG00000065526 0.42560112
ENSG00000116809 0.23665191
ENSG00000173641 0.21571026
ENSG00000142627 0.26486540
ENSG00000142632 0.39745487
ENSG00000037637 0.39225604
ENSG00000055070 0.35875832
ENSG00000157191 0.40249931
ENSG00000219481 0.17027898
ENSG00000215908 0.34871045
ENSG00000058453 0.42680403
ENSG00000117122 0.45974580
ENSG00000159363 0.12553041
ENSG00000117118 0.25668715
ENSG00000179051 0.47466712
ENSG00000074964 0.41228712
ENSG00000159423 0.36322343
ENSG00000169991 0.24337731
ENSG00000127481 0.37286868
ENSG00000127463 0.06454038
ENSG00000053372 0.26809289
ENSG00000053371 0.46860815
ENSG00000040487 0.40934588
ENSG00000077549 0.42550079
ENSG00000173436 0.38562147
ENSG00000158747 0.18612777
ENSG00000169914 0.09540710
ENSG00000188257 0.43878268
ENSG00000127472 0.45170947
ENSG00000162545 0.21296811
ENSG00000090432 0.42592720
ENSG00000158828 0.43873436
ENSG00000117242 0.46129799
ENSG00000244038 0.10875333
ENSG00000117245 0.35836812
ENSG00000189410 0.33138548
ENSG00000127483 0.44808081
ENSG00000075151 0.32216822
ENSG00000117298 0.38792063
ENSG00000142794 0.46190011
ENSG00000162551 0.41401897
ENSG00000090686 0.24206480
ENSG00000142798 0.18996239
ENSG00000070831 0.46699098
ENSG00000162552 0.31482589
ENSG00000184677 0.46368687
ENSG00000133216 0.37872665
ENSG00000004487 0.38913159
ENSG00000169641 0.45182682
ENSG00000179546 0.30547385
ENSG00000125944 0.32273720
ENSG00000125945 0.29632063
ENSG00000088280 0.34886481
ENSG00000117318 0.42078350
ENSG00000142676 0.31914633
ENSG00000011007 0.38059627
ENSG00000057757 0.39042159
ENSG00000011009 0.37847063
ENSG00000117308 0.46778692
ENSG00000117305 0.38358139
ENSG00000179163 0.39983890
ENSG00000189266 0.11494881
ENSG00000188529 0.15241580
ENSG00000001460 0.38152855
ENSG00000001461 0.04413959
ENSG00000117602 0.12971264
ENSG00000133226 0.39250181
ENSG00000169504 0.09475027
ENSG00000117614 0.41179424
ENSG00000117616 0.36633579
ENSG00000183726 0.34799955
ENSG00000204178 0.47413441
ENSG00000157978 0.08379366
ENSG00000117643 0.43822670
ENSG00000162430 0.34697971
ENSG00000117640 0.18338534
ENSG00000182749 0.47481281
ENSG00000117632 0.33860550
ENSG00000158006 0.43764568
ENSG00000158008 0.21646952
ENSG00000158022 0.39876917
ENSG00000130695 0.26519304
ENSG00000142669 0.46120534
ENSG00000158062 0.41373505
ENSG00000117682 0.05511682
ENSG00000198830 0.30891942
ENSG00000117713 0.15646307
ENSG00000060642 0.38090732
ENSG00000204160 0.34997462
ENSG00000142751 0.47221651
ENSG00000198746 0.37755997
ENSG00000090273 0.21439389
ENSG00000253368 0.23942564
ENSG00000158246 0.31585426
ENSG00000090020 0.36129821
ENSG00000142784 0.45334858
ENSG00000186501 0.41329592
ENSG00000142733 0.33885838
ENSG00000158195 0.45177989
ENSG00000126705 0.24571204
ENSG00000126709 0.40363829
ENSG00000009780 0.44665809
ENSG00000117758 0.30434160
ENSG00000117751 0.43062564
ENSG00000117748 0.47486139
ENSG00000158156 0.19254801
ENSG00000158161 0.21817933
ENSG00000126698 0.44786769
ENSG00000130770 0.06742359
ENSG00000130766 0.35279814
ENSG00000130772 0.39370019
ENSG00000204138 0.30769264
ENSG00000180198 0.29450897
ENSG00000242125 0.44250927
ENSG00000180098 0.37450306
ENSG00000197989 0.29061793
ENSG00000120656 0.33977584
ENSG00000162419 0.44651551
ENSG00000198492 0.43343137
ENSG00000159023 0.40666985
ENSG00000253304 0.35755568
ENSG00000116350 0.35334756
ENSG00000116353 0.33089934
ENSG00000060656 0.31830224
ENSG00000162512 0.14974376
ENSG00000134644 0.36466547
ENSG00000060688 0.34975906
ENSG00000121766 0.45967629
ENSG00000121769 0.33222506
ENSG00000168528 0.37861290
ENSG00000162517 0.20320958
ENSG00000084636 0.44763072
ENSG00000121753 0.32263694
ENSG00000134668 0.29139625
ENSG00000184007 0.28751331
ENSG00000121774 0.30395125
ENSG00000121775 0.21260963
ENSG00000025800 0.43960308
ENSG00000084652 0.11465237
ENSG00000160050 0.35129649
ENSG00000160055 0.46470874
ENSG00000084623 0.47793731
ENSG00000220785 0.45201452
ENSG00000116478 0.45037023
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ENSG00000160062 0.29927508
ENSG00000176261 0.30416428
ENSG00000162521 0.19802293
ENSG00000162520 0.29751218
ENSG00000162522 0.47357992
ENSG00000134684 0.46771669
ENSG00000116497 0.19615226
ENSG00000160097 0.41725015
ENSG00000116514 0.44630154
ENSG00000004455 0.31975237
ENSG00000142920 0.34935847
ENSG00000116525 0.41078073
ENSG00000160094 0.25365391
ENSG00000134686 0.32953314
ENSG00000121903 0.42810145
ENSG00000121904 0.33958590
ENSG00000163866 0.47832187
ENSG00000163867 0.24818964
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ENSG00000116560 0.47197825
ENSG00000146463 0.43475275
ENSG00000142687 0.20278017
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ENSG00000126067 0.47878839
ENSG00000142686 0.38723013
ENSG00000134698 0.36490021
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ENSG00000196182 0.22103915
ENSG00000181817 0.14112350
ENSG00000116898 0.39454113
ENSG00000163874 0.34648527
ENSG00000163875 0.24753016
ENSG00000163877 0.32119584
ENSG00000134697 0.35015932
ENSG00000116922 0.35074328
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ENSG00000197982 0.31205785
ENSG00000188786 0.14080637
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ENSG00000183431 0.40133037
ENSG00000183386 0.36896169
ENSG00000183520 0.30327522
ENSG00000116954 0.33730861
ENSG00000214114 0.42396678
ENSG00000174574 0.25442665
ENSG00000168653 0.10439166
ENSG00000127603 0.25964170
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ENSG00000084072 0.32655132
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ENSG00000066136 0.33093175
ENSG00000171793 0.21812892
ENSG00000010803 0.45944447
ENSG00000127124 0.43614155
ENSG00000198815 0.47796272
ENSG00000127125 0.43323513
ENSG00000171960 0.29570617
ENSG00000065978 0.31327253
ENSG00000117385 0.42497324
ENSG00000164010 0.31561480
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ENSG00000117394 0.42123947
ENSG00000117395 0.37547537
ENSG00000066322 0.44200635
ENSG00000159479 0.46099690
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ENSG00000132768 0.26014539
ENSG00000117410 0.22421128
ENSG00000117411 0.27937674
ENSG00000196517 0.31077108
ENSG00000178028 0.19271903
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ENSG00000187147 0.38203557
ENSG00000126106 0.38346920
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ENSG00000173846 0.17285643
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ENSG00000197429 0.39258367
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ENSG00000085998 0.47442448
ENSG00000132128 0.33428054
ENSG00000173660 0.35101993
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ENSG00000079277 0.29906000
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ENSG00000123472 0.45199106
ENSG00000159658 0.46531265
ENSG00000123473 0.24517629
ENSG00000162368 0.28938775
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ENSG00000117862 0.46484808
ENSG00000198841 0.46957125
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ENSG00000157077 0.14576796
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ENSG00000174332 0.34567888
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ENSG00000116209 0.44128665
ENSG00000116205 0.39640537
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ENSG00000162390 0.39198263
ENSG00000116133 0.46561492
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ENSG00000162402 0.27365410
ENSG00000162407 0.29150712
ENSG00000162600 0.28702259
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ENSG00000116641 0.14921553
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ENSG00000162434 0.20234868
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ENSG00000162616 0.09630406
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ENSG00000171502 0.35878832
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ENSG00000171488 0.33183780
ENSG00000171492 0.28550749
ENSG00000162664 0.39817937
ENSG00000122482 0.42617802
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ENSG00000174842 0.45502934
ENSG00000122484 0.41272731
ENSG00000067208 0.47931735
ENSG00000122406 0.37131086
ENSG00000154511 0.26829633
ENSG00000143033 0.11445385
ENSG00000117500 0.07418527
ENSG00000117505 0.07543804
ENSG00000137942 0.23228473
ENSG00000137936 0.24432411
ENSG00000067334 0.38183852
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ENSG00000137962 0.37490347
ENSG00000117528 0.40955498
ENSG00000117525 0.26048051
ENSG00000117519 0.35340850
ENSG00000235501 0.34480716
ENSG00000172339 0.30975889
ENSG00000117569 0.39632451
ENSG00000162627 0.06899292
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ENSG00000162688 0.42002291
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ENSG00000122435 0.32549484
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ENSG00000117543 0.34818501
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ENSG00000198890 0.43355396
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ENSG00000162636 0.29132234
ENSG00000134186 0.16305103
ENSG00000116266 0.18169776
ENSG00000121940 0.32589148
ENSG00000085433 0.24238176
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ENSG00000130544 0.41210568
ENSG00000171105 0.43470394
ENSG00000198816 0.34988227
ENSG00000090674 0.45065236
ENSG00000032444 0.39236531
ENSG00000076924 0.40882250
ENSG00000142459 0.17913801
ENSG00000076984 0.20977500
ENSG00000104976 0.43139047
ENSG00000178531 0.39211939
ENSG00000104980 0.46331552
ENSG00000066044 0.41811740
ENSG00000090661 0.45044080
ENSG00000167775 0.27544214
ENSG00000233927 0.42149256
ENSG00000167772 0.33517663
ENSG00000185236 0.40456314
ENSG00000099785 0.40023894
ENSG00000099783 0.11621963
ENSG00000133250 0.30884939
ENSG00000142303 0.41252028
ENSG00000167785 0.30674309
ENSG00000130803 0.45318482
ENSG00000196110 0.41872841
ENSG00000188321 0.26453762
ENSG00000174652 0.43421530
ENSG00000130818 0.33421647
ENSG00000197961 0.45547211
ENSG00000171469 0.22553696
ENSG00000267106 0.30979385
ENSG00000171466 0.46710806
ENSG00000196605 0.22539007
ENSG00000127452 0.39047639
ENSG00000198258 0.47126659
ENSG00000127445 0.33083449
ENSG00000105088 0.42447052
ENSG00000130813 0.41080676
ENSG00000130811 0.43281784
ENSG00000130816 0.45018201
ENSG00000267534 0.36067334
ENSG00000105364 0.17814912
ENSG00000090339 0.28209441
ENSG00000105376 0.34536795
ENSG00000161847 0.37453167
ENSG00000105397 0.37473186
ENSG00000105401 0.29805302
ENSG00000065989 0.36756339
ENSG00000079999 0.35474572
ENSG00000180739 0.41617690
ENSG00000130734 0.31118912
ENSG00000129347 0.42700410
ENSG00000129355 0.32897839
ENSG00000129353 0.19123227
ENSG00000267100 0.45264688
ENSG00000129351 0.10117799
ENSG00000213339 0.32891300
ENSG00000079805 0.23133971
ENSG00000099203 0.45816858
ENSG00000142453 0.41025972
ENSG00000130733 0.07288590
ENSG00000142444 0.34806137
ENSG00000127616 0.23520156
ENSG00000130164 0.43005785
ENSG00000197256 0.35734465
ENSG00000130158 0.25672950
ENSG00000105518 0.40656466
ENSG00000105520 0.22826597
ENSG00000187266 0.37003587
ENSG00000130175 0.47539777
ENSG00000130159 0.43338231
ENSG00000130176 0.29830518
ENSG00000130165 0.38014399
ENSG00000198551 0.31111949
ENSG00000197044 0.44344134
ENSG00000171295 0.32166537
ENSG00000198429 0.21727422
ENSG00000219665 0.38456203
ENSG00000196646 0.45610092
ENSG00000197857 0.36864182
ENSG00000173875 0.37952128
ENSG00000104774 0.26878402
ENSG00000105583 0.37301226
ENSG00000095059 0.35598675
ENSG00000132004 0.41223530
ENSG00000105576 0.12813618
ENSG00000123144 0.28706040
ENSG00000198356 0.46521970
ENSG00000095066 0.26547923
ENSG00000171223 0.24015703
ENSG00000167815 0.40055877
ENSG00000105612 0.47473995
ENSG00000105607 0.13631467
ENSG00000179115 0.19868267
ENSG00000179218 0.11244843
ENSG00000179262 0.34911166
ENSG00000179271 0.39017486
ENSG00000008441 0.43153838
ENSG00000104907 0.43974763
ENSG00000160877 0.32456732
ENSG00000104915 0.25528389
ENSG00000160888 0.29657327
ENSG00000104957 0.39280187
ENSG00000037757 0.38192298
ENSG00000104979 0.34377663
ENSG00000132003 0.35818788
ENSG00000132024 0.12036671
ENSG00000132000 0.39954513
ENSG00000132017 0.45095777
ENSG00000132005 0.39694386
ENSG00000141858 0.29465435
ENSG00000072062 0.43316149
ENSG00000072071 0.16781185
ENSG00000123146 0.38442223
ENSG00000123136 0.44508790
ENSG00000123143 0.43453305
ENSG00000123159 0.16115614
ENSG00000132002 0.14420019
ENSG00000099797 0.29009658
ENSG00000099795 0.19818795
ENSG00000160961 0.44836476
ENSG00000105137 0.34261547
ENSG00000105135 0.29367122
ENSG00000074181 0.38498083
ENSG00000141867 0.45229580
ENSG00000105127 0.44188648
ENSG00000011243 0.22884811
ENSG00000011451 0.15553210
ENSG00000167460 0.25778981
ENSG00000167461 0.46931276
ENSG00000105058 0.25318116
ENSG00000072958 0.30628522
ENSG00000127528 0.30744661
ENSG00000127527 0.41760748
ENSG00000085872 0.22966818
ENSG00000127526 0.32146404
ENSG00000214046 0.47004144
ENSG00000127511 0.43151374
ENSG00000099331 0.23430960
ENSG00000053501 0.35295094
ENSG00000099330 0.30863923
ENSG00000160113 0.35974791
ENSG00000105393 0.46867861
ENSG00000127220 0.36150633
ENSG00000130312 0.32526327
ENSG00000130311 0.33115879
ENSG00000074855 0.32920699
ENSG00000130299 0.36919409
ENSG00000141971 0.29895234
ENSG00000130304 0.13372692
ENSG00000130313 0.27532336
ENSG00000130309 0.32874963
ENSG00000130479 0.33559139
ENSG00000105640 0.37113712
ENSG00000007080 0.31941576
ENSG00000105643 0.28081673
ENSG00000099308 0.45463314
ENSG00000254858 0.20191556
ENSG00000130522 0.39527179
ENSG00000130520 0.28976074
ENSG00000130517 0.41885555
ENSG00000130513 0.21742479
ENSG00000130511 0.30480206
ENSG00000105655 0.42519945
ENSG00000105656 0.42045404
ENSG00000105701 0.20597040
ENSG00000105700 0.38341310
ENSG00000221983 0.37513224
ENSG00000006015 0.47001793
ENSG00000167487 0.29909586
ENSG00000105662 0.41466304
ENSG00000105664 0.32933746
ENSG00000005007 0.41254618
ENSG00000105669 0.36233693
ENSG00000105671 0.27820211
ENSG00000051128 0.23355604
ENSG00000064607 0.44734624
ENSG00000105676 0.19598563
ENSG00000181035 0.28709818
ENSG00000064545 0.19692472
ENSG00000254901 0.23477939
ENSG00000064490 0.39586581
ENSG00000105705 0.18826616
ENSG00000129933 0.11153881
ENSG00000167491 0.29147017
ENSG00000186010 0.44121465
ENSG00000160161 0.43269497
ENSG00000089639 0.31386074
ENSG00000105726 0.43330873
ENSG00000181896 0.27173379
ENSG00000081665 0.35301201
ENSG00000184635 0.32076014
ENSG00000188171 0.31646886
ENSG00000105750 0.24629068
ENSG00000118620 0.43477717
ENSG00000160352 0.44389499
ENSG00000196705 0.26817429
ENSG00000182141 0.39780384
ENSG00000172687 0.44796244
ENSG00000198521 0.34333796
ENSG00000167232 0.45022908
ENSG00000213096 0.44159860
ENSG00000268362 0.44207826
ENSG00000261824 0.41365755
ENSG00000169021 0.39812572
ENSG00000105171 0.29356185
ENSG00000166289 0.26511116
ENSG00000131943 0.40347948
ENSG00000105173 0.32783307
ENSG00000105176 0.35172634
ENSG00000121297 0.40758950
ENSG00000168813 0.22937952
ENSG00000178904 0.47022950
ENSG00000105185 0.22607546
ENSG00000105186 0.21368320
ENSG00000121289 0.12103050
ENSG00000076650 0.32360410
ENSG00000130881 0.41332176
ENSG00000153879 0.11415423
ENSG00000124299 0.18428150
ENSG00000153885 0.27060812
ENSG00000257103 0.41592084
ENSG00000166398 0.46122849
ENSG00000105220 0.38394603
ENSG00000126261 0.11334893
ENSG00000142279 0.15978284
ENSG00000089335 0.28837789
ENSG00000197841 0.40611752
ENSG00000153896 0.43060097
ENSG00000089351 0.47266959
ENSG00000105711 0.40164960
ENSG00000089327 0.24369328
ENSG00000105698 0.43885515
ENSG00000105677 0.45953721
ENSG00000126254 0.19741827
ENSG00000126267 0.18808918
ENSG00000272333 0.42257915
ENSG00000205155 0.40432555
ENSG00000004776 0.34626845
ENSG00000167595 0.25459846
ENSG00000105290 0.31191898
ENSG00000126243 0.45802929
ENSG00000205138 0.42901953
ENSG00000105270 0.41704588
ENSG00000161277 0.37758843
ENSG00000105254 0.41319261
ENSG00000126247 0.15517157
ENSG00000167635 0.17342954
ENSG00000232677 0.22671072
ENSG00000186017 0.40829843
ENSG00000254004 0.24566742
ENSG00000186020 0.27777048
ENSG00000197808 0.31254406
ENSG00000189042 0.35489741
ENSG00000267041 0.34094543
ENSG00000198453 0.45344212
ENSG00000197050 0.39831347
ENSG00000196967 0.45918951
ENSG00000245680 0.30751694
ENSG00000188283 0.38240156
ENSG00000181666 0.40204828
ENSG00000189164 0.43458189
ENSG00000196437 0.42060610
ENSG00000171827 0.17215258
ENSG00000188227 0.43384807
ENSG00000120784 0.28035096
ENSG00000198182 0.36689158
ENSG00000105738 0.36721282
ENSG00000167642 0.17614357
ENSG00000167641 0.32822618
ENSG00000167645 0.16378494
ENSG00000099337 0.14056761
ENSG00000099341 0.47427934
ENSG00000188766 0.19912328
ENSG00000130244 0.27925946
ENSG00000178982 0.45960677
ENSG00000130402 0.21611609
ENSG00000104823 0.25766293
ENSG00000104824 0.14761479
ENSG00000068903 0.47190681
ENSG00000104825 0.26756343
ENSG00000128626 0.40737921
ENSG00000269190 0.23445075
ENSG00000130669 0.36186269
ENSG00000179134 0.29220736
ENSG00000006712 0.11680487
ENSG00000063322 0.18737909
ENSG00000128016 0.39627058
ENSG00000090924 0.28089421
ENSG00000105193 0.45833103
ENSG00000196235 0.11123222
ENSG00000105197 0.38514817
ENSG00000176396 0.34583421
ENSG00000105204 0.28886427
ENSG00000105202 0.47367617
ENSG00000013275 0.39659394
ENSG00000128000 0.35812777
ENSG00000197782 0.43431315
ENSG00000130758 0.23609127
ENSG00000105221 0.17708934
ENSG00000160392 0.25171791
ENSG00000105223 0.28359798
ENSG00000105227 0.45149815
ENSG00000197019 0.11385430
ENSG00000167565 0.33524051
ENSG00000090013 0.32881500
ENSG00000160410 0.38617734
ENSG00000090006 0.36141716
ENSG00000105245 0.23195492
ENSG00000123815 0.23910451
ENSG00000086544 0.07523348
ENSG00000188493 0.40252582
ENSG00000077312 0.37761689
ENSG00000167601 0.22908997
ENSG00000105323 0.11892464
ENSG00000105329 0.35935847
ENSG00000142039 0.34113472
ENSG00000077348 0.44916831
ENSG00000105341 0.37576148
ENSG00000105372 0.34707241
ENSG00000076928 0.18463808
ENSG00000105404 0.08827321
ENSG00000105732 0.44332189
ENSG00000160570 0.39946496
ENSG00000167625 0.34044000
ENSG00000105723 0.30441248
ENSG00000105722 0.45135721
ENSG00000079432 0.11758325
ENSG00000105429 0.18920476
ENSG00000176531 0.20748941
ENSG00000105755 0.45088727
ENSG00000073050 0.38885679
ENSG00000167378 0.34305555
ENSG00000124444 0.40743179
ENSG00000131116 0.38237342
ENSG00000011422 0.27333487
ENSG00000105771 0.42179615
ENSG00000167637 0.34050325
ENSG00000176222 0.40419346
ENSG00000124459 0.28489833
ENSG00000159885 0.26535661
ENSG00000267680 0.36715444
ENSG00000256294 0.33825780
ENSG00000263002 0.18902931
ENSG00000167380 0.34716510
ENSG00000131115 0.29559757
ENSG00000062370 0.28182345
ENSG00000278318 0.21454563
ENSG00000167384 0.41742887
ENSG00000073008 0.41964259
ENSG00000279095 0.33073718
ENSG00000069399 0.40045221
ENSG00000130202 0.31465383
ENSG00000130204 0.37384431
ENSG00000130203 0.40682765
ENSG00000104853 0.37556124
ENSG00000104856 0.28946241
ENSG00000104859 0.26951535
ENSG00000142252 0.31212725
ENSG00000007047 0.39521773
ENSG00000104866 0.45046431
ENSG00000007255 0.31840374
ENSG00000189114 0.37698996
ENSG00000104884 0.30667271
ENSG00000104881 0.34505660
ENSG00000012061 0.24805793
ENSG00000125744 0.19620774
ENSG00000125753 0.37421680
ENSG00000125741 0.37498926
ENSG00000125746 0.25592334
ENSG00000125743 0.31489468
ENSG00000011478 0.46851421
ENSG00000177051 0.31506644
ENSG00000177045 0.31887654
ENSG00000104936 0.30600318
ENSG00000185800 0.24704518
ENSG00000125755 0.31468823
ENSG00000170604 0.17783857
ENSG00000176182 0.36360654
ENSG00000188624 0.22499762
ENSG00000204866 0.22243055
ENSG00000124440 0.35983768
ENSG00000011485 0.38380583
ENSG00000169515 0.19283279
ENSG00000182013 0.42303360
ENSG00000160014 0.39605482
ENSG00000197380 0.41594645
ENSG00000105287 0.43328420
ENSG00000090372 0.29772875
ENSG00000181027 0.27516503
ENSG00000105281 0.40598595
ENSG00000042753 0.47809043
ENSG00000160007 0.18109176
ENSG00000130751 0.43313694
ENSG00000130748 0.42424395
ENSG00000130749 0.31337652
ENSG00000142230 0.27281562
ENSG00000105327 0.41732676
ENSG00000105321 0.32605655
ENSG00000257704 0.40772086
ENSG00000134815 0.41046823
ENSG00000105419 0.39364587
ENSG00000105402 0.28897650
ENSG00000063169 0.31748978
ENSG00000024422 0.44039747
ENSG00000105373 0.43436205
ENSG00000178980 0.39157217
ENSG00000105499 0.35200225
ENSG00000105486 0.25296615
ENSG00000185453 0.36779693
ENSG00000105483 0.45131020
ENSG00000142227 0.13509652
ENSG00000105438 0.44725141
ENSG00000105464 0.45227237
ENSG00000105447 0.27571937
ENSG00000105443 0.46287456
ENSG00000063177 0.46907782
ENSG00000063176 0.14807456
ENSG00000105552 0.46727193
ENSG00000087076 0.24827745
ENSG00000087074 0.38248593
ENSG00000104805 0.15197325
ENSG00000087088 0.10325969
ENSG00000087086 0.39975881
ENSG00000104812 0.45990804
ENSG00000183207 0.11475146
ENSG00000104852 0.24664651
ENSG00000104863 0.33038001
ENSG00000177380 0.24333221
ENSG00000130529 0.33654598
ENSG00000074219 0.30898944
ENSG00000104872 0.45372262
ENSG00000161618 0.33319051
ENSG00000142541 0.46731874
ENSG00000142534 0.46703781
ENSG00000104870 0.38935126
ENSG00000142552 0.21029198
ENSG00000142546 0.37926621
ENSG00000126464 0.24593475
ENSG00000126458 0.25985156
ENSG00000126461 0.23988309
ENSG00000126456 0.43640882
ENSG00000126453 0.28444000
ENSG00000126457 0.16122438
ENSG00000196961 0.47119561
ENSG00000010361 0.42757843
ENSG00000104973 0.29963277
ENSG00000104960 0.29812507
ENSG00000039650 0.38863672
ENSG00000204673 0.46680375
ENSG00000104946 0.33280480
ENSG00000213024 0.13266553
ENSG00000169136 0.41062452
ENSG00000105053 0.25489877
ENSG00000142528 0.22371811
ENSG00000131408 0.42825065
ENSG00000062822 0.35419937
ENSG00000161671 0.32367066
ENSG00000161677 0.41098885
ENSG00000167747 0.17507315
ENSG00000142544 0.36695000
ENSG00000105379 0.19774779
ENSG00000105497 0.42197303
ENSG00000182310 0.31971872
ENSG00000198093 0.45612418
ENSG00000176024 0.28221040
ENSG00000256683 0.42976155
ENSG00000197619 0.30494370
ENSG00000142556 0.26592964
ENSG00000256087 0.45114570
ENSG00000197608 0.36487087
ENSG00000204611 0.44379948
ENSG00000105568 0.41106689
ENSG00000196214 0.46044108
ENSG00000198464 0.38324476
ENSG00000167554 0.40424630
ENSG00000221923 0.43303875
ENSG00000167555 0.41161252
ENSG00000167766 0.29916760
ENSG00000213020 0.25622093
ENSG00000189190 0.39391747
ENSG00000198538 0.12150177
ENSG00000204604 0.17336585
ENSG00000213793 0.45939809
ENSG00000180257 0.43406844
ENSG00000242779 0.34154498
ENSG00000170949 0.29794504
[ reached 'max' / getOption("max.print") -- omitted 448 rows ]
#visualize what the permutations look like (what the scrambled labels look like)
for(time in 1:6){
print(permuted_results[[time]])
}
Sample_ID Individual Sex Replicate treatment RIN
18855_3_S 18855_3_S NA18855 F 3 Unstrain 10.0
19160_3_S 19160_3_S NA19160 M 3 Strain 10.0
18856_3_U 18856_3_U NA18856 M 3 Unstrain 10.0
18856_1_U 18856_1_U NA18856 M 1 Unstrain 9.9
18855_2_S 18855_2_S NA18855 F 2 Strain 10.0
18856_2_S 18856_2_S NA18856 M 2 Unstrain 9.6
19160_3_U 19160_3_U NA19160 M 3 Unstrain 10.0
18855_2_U 18855_2_U NA18855 F 2 Strain 10.0
19160_2_S 19160_2_S NA19160 M 2 Strain 10.0
18855_1_S 18855_1_S NA18855 F 1 Unstrain 9.9
18856_1_S 18856_1_S NA18856 M 1 Unstrain 8.5
19160_1_S 19160_1_S NA19160 M 1 Strain 9.9
19160_2_U 19160_2_U NA19160 M 2 Strain 10.0
18855_1_U 18855_1_U NA18855 F 1 Strain 10.0
18856_3_S 18856_3_S NA18856 M 3 Strain 9.6
18856_2_U 18856_2_U NA18856 M 2 Strain 9.5
18855_3_U 18855_3_U NA18855 F 3 Unstrain 10.0
LibraryPrepBatch LibSize W_1 W_2
18855_3_S 2 15419569 0.6403178 -1.0334196
19160_3_S 2 15177653 0.4887528 -1.3355831
18856_3_U 2 13722909 0.9502388 -0.7892404
18856_1_U 1 18647599 1.2228123 -1.4496449
18855_2_S 1 18256163 0.8233400 -0.6748150
18856_2_S 1 16782764 0.0515988 -1.2784836
19160_3_U 2 19839604 0.3406102 -1.4859733
18855_2_U 1 18618691 0.7609177 -0.8596946
19160_2_S 1 16622407 0.4247172 -1.4414851
18855_1_S 1 19712744 0.7356719 -1.4499412
18856_1_S 1 13571439 1.1409773 -1.1686123
19160_1_S 1 20979958 0.5909682 -1.9538068
19160_2_U 1 20924281 0.1295380 -1.8537818
18855_1_U 1 20146697 0.7569979 -1.3228202
18856_3_S 2 15807863 0.8205467 -0.8981659
18856_2_U 1 15142152 0.4572116 -1.0041098
18855_3_U 2 18157918 0.5909283 -1.1455295
Sample_ID Individual Sex Replicate treatment RIN
18855_3_S 18855_3_S NA18855 F 3 Strain 10.0
19160_3_S 19160_3_S NA19160 M 3 Strain 10.0
18856_3_U 18856_3_U NA18856 M 3 Unstrain 10.0
18856_1_U 18856_1_U NA18856 M 1 Unstrain 9.9
18855_2_S 18855_2_S NA18855 F 2 Unstrain 10.0
18856_2_S 18856_2_S NA18856 M 2 Strain 9.6
19160_3_U 19160_3_U NA19160 M 3 Strain 10.0
18855_2_U 18855_2_U NA18855 F 2 Strain 10.0
19160_2_S 19160_2_S NA19160 M 2 Strain 10.0
18855_1_S 18855_1_S NA18855 F 1 Strain 9.9
18856_1_S 18856_1_S NA18856 M 1 Strain 8.5
19160_1_S 19160_1_S NA19160 M 1 Unstrain 9.9
19160_2_U 19160_2_U NA19160 M 2 Unstrain 10.0
18855_1_U 18855_1_U NA18855 F 1 Strain 10.0
18856_3_S 18856_3_S NA18856 M 3 Unstrain 9.6
18856_2_U 18856_2_U NA18856 M 2 Unstrain 9.5
18855_3_U 18855_3_U NA18855 F 3 Unstrain 10.0
LibraryPrepBatch LibSize W_1 W_2
18855_3_S 2 15419569 0.6403178 -1.0334196
19160_3_S 2 15177653 0.4887528 -1.3355831
18856_3_U 2 13722909 0.9502388 -0.7892404
18856_1_U 1 18647599 1.2228123 -1.4496449
18855_2_S 1 18256163 0.8233400 -0.6748150
18856_2_S 1 16782764 0.0515988 -1.2784836
19160_3_U 2 19839604 0.3406102 -1.4859733
18855_2_U 1 18618691 0.7609177 -0.8596946
19160_2_S 1 16622407 0.4247172 -1.4414851
18855_1_S 1 19712744 0.7356719 -1.4499412
18856_1_S 1 13571439 1.1409773 -1.1686123
19160_1_S 1 20979958 0.5909682 -1.9538068
19160_2_U 1 20924281 0.1295380 -1.8537818
18855_1_U 1 20146697 0.7569979 -1.3228202
18856_3_S 2 15807863 0.8205467 -0.8981659
18856_2_U 1 15142152 0.4572116 -1.0041098
18855_3_U 2 18157918 0.5909283 -1.1455295
Sample_ID Individual Sex Replicate treatment RIN
18855_3_S 18855_3_S NA18855 F 3 Strain 10.0
19160_3_S 19160_3_S NA19160 M 3 Strain 10.0
18856_3_U 18856_3_U NA18856 M 3 Strain 10.0
18856_1_U 18856_1_U NA18856 M 1 Strain 9.9
18855_2_S 18855_2_S NA18855 F 2 Unstrain 10.0
18856_2_S 18856_2_S NA18856 M 2 Unstrain 9.6
19160_3_U 19160_3_U NA19160 M 3 Unstrain 10.0
18855_2_U 18855_2_U NA18855 F 2 Strain 10.0
19160_2_S 19160_2_S NA19160 M 2 Unstrain 10.0
18855_1_S 18855_1_S NA18855 F 1 Strain 9.9
18856_1_S 18856_1_S NA18856 M 1 Strain 8.5
19160_1_S 19160_1_S NA19160 M 1 Strain 9.9
19160_2_U 19160_2_U NA19160 M 2 Unstrain 10.0
18855_1_U 18855_1_U NA18855 F 1 Unstrain 10.0
18856_3_S 18856_3_S NA18856 M 3 Unstrain 9.6
18856_2_U 18856_2_U NA18856 M 2 Unstrain 9.5
18855_3_U 18855_3_U NA18855 F 3 Strain 10.0
LibraryPrepBatch LibSize W_1 W_2
18855_3_S 2 15419569 0.6403178 -1.0334196
19160_3_S 2 15177653 0.4887528 -1.3355831
18856_3_U 2 13722909 0.9502388 -0.7892404
18856_1_U 1 18647599 1.2228123 -1.4496449
18855_2_S 1 18256163 0.8233400 -0.6748150
18856_2_S 1 16782764 0.0515988 -1.2784836
19160_3_U 2 19839604 0.3406102 -1.4859733
18855_2_U 1 18618691 0.7609177 -0.8596946
19160_2_S 1 16622407 0.4247172 -1.4414851
18855_1_S 1 19712744 0.7356719 -1.4499412
18856_1_S 1 13571439 1.1409773 -1.1686123
19160_1_S 1 20979958 0.5909682 -1.9538068
19160_2_U 1 20924281 0.1295380 -1.8537818
18855_1_U 1 20146697 0.7569979 -1.3228202
18856_3_S 2 15807863 0.8205467 -0.8981659
18856_2_U 1 15142152 0.4572116 -1.0041098
18855_3_U 2 18157918 0.5909283 -1.1455295
Sample_ID Individual Sex Replicate treatment RIN
18855_3_S 18855_3_S NA18855 F 3 Unstrain 10.0
19160_3_S 19160_3_S NA19160 M 3 Unstrain 10.0
18856_3_U 18856_3_U NA18856 M 3 Unstrain 10.0
18856_1_U 18856_1_U NA18856 M 1 Unstrain 9.9
18855_2_S 18855_2_S NA18855 F 2 Strain 10.0
18856_2_S 18856_2_S NA18856 M 2 Unstrain 9.6
19160_3_U 19160_3_U NA19160 M 3 Strain 10.0
18855_2_U 18855_2_U NA18855 F 2 Strain 10.0
19160_2_S 19160_2_S NA19160 M 2 Strain 10.0
18855_1_S 18855_1_S NA18855 F 1 Strain 9.9
18856_1_S 18856_1_S NA18856 M 1 Strain 8.5
19160_1_S 19160_1_S NA19160 M 1 Unstrain 9.9
19160_2_U 19160_2_U NA19160 M 2 Unstrain 10.0
18855_1_U 18855_1_U NA18855 F 1 Strain 10.0
18856_3_S 18856_3_S NA18856 M 3 Strain 9.6
18856_2_U 18856_2_U NA18856 M 2 Unstrain 9.5
18855_3_U 18855_3_U NA18855 F 3 Strain 10.0
LibraryPrepBatch LibSize W_1 W_2
18855_3_S 2 15419569 0.6403178 -1.0334196
19160_3_S 2 15177653 0.4887528 -1.3355831
18856_3_U 2 13722909 0.9502388 -0.7892404
18856_1_U 1 18647599 1.2228123 -1.4496449
18855_2_S 1 18256163 0.8233400 -0.6748150
18856_2_S 1 16782764 0.0515988 -1.2784836
19160_3_U 2 19839604 0.3406102 -1.4859733
18855_2_U 1 18618691 0.7609177 -0.8596946
19160_2_S 1 16622407 0.4247172 -1.4414851
18855_1_S 1 19712744 0.7356719 -1.4499412
18856_1_S 1 13571439 1.1409773 -1.1686123
19160_1_S 1 20979958 0.5909682 -1.9538068
19160_2_U 1 20924281 0.1295380 -1.8537818
18855_1_U 1 20146697 0.7569979 -1.3228202
18856_3_S 2 15807863 0.8205467 -0.8981659
18856_2_U 1 15142152 0.4572116 -1.0041098
18855_3_U 2 18157918 0.5909283 -1.1455295
Sample_ID Individual Sex Replicate treatment RIN
18855_3_S 18855_3_S NA18855 F 3 Unstrain 10.0
19160_3_S 19160_3_S NA19160 M 3 Unstrain 10.0
18856_3_U 18856_3_U NA18856 M 3 Unstrain 10.0
18856_1_U 18856_1_U NA18856 M 1 Unstrain 9.9
18855_2_S 18855_2_S NA18855 F 2 Unstrain 10.0
18856_2_S 18856_2_S NA18856 M 2 Strain 9.6
19160_3_U 19160_3_U NA19160 M 3 Strain 10.0
18855_2_U 18855_2_U NA18855 F 2 Unstrain 10.0
19160_2_S 19160_2_S NA19160 M 2 Strain 10.0
18855_1_S 18855_1_S NA18855 F 1 Unstrain 9.9
18856_1_S 18856_1_S NA18856 M 1 Unstrain 8.5
19160_1_S 19160_1_S NA19160 M 1 Strain 9.9
19160_2_U 19160_2_U NA19160 M 2 Strain 10.0
18855_1_U 18855_1_U NA18855 F 1 Strain 10.0
18856_3_S 18856_3_S NA18856 M 3 Strain 9.6
18856_2_U 18856_2_U NA18856 M 2 Strain 9.5
18855_3_U 18855_3_U NA18855 F 3 Strain 10.0
LibraryPrepBatch LibSize W_1 W_2
18855_3_S 2 15419569 0.6403178 -1.0334196
19160_3_S 2 15177653 0.4887528 -1.3355831
18856_3_U 2 13722909 0.9502388 -0.7892404
18856_1_U 1 18647599 1.2228123 -1.4496449
18855_2_S 1 18256163 0.8233400 -0.6748150
18856_2_S 1 16782764 0.0515988 -1.2784836
19160_3_U 2 19839604 0.3406102 -1.4859733
18855_2_U 1 18618691 0.7609177 -0.8596946
19160_2_S 1 16622407 0.4247172 -1.4414851
18855_1_S 1 19712744 0.7356719 -1.4499412
18856_1_S 1 13571439 1.1409773 -1.1686123
19160_1_S 1 20979958 0.5909682 -1.9538068
19160_2_U 1 20924281 0.1295380 -1.8537818
18855_1_U 1 20146697 0.7569979 -1.3228202
18856_3_S 2 15807863 0.8205467 -0.8981659
18856_2_U 1 15142152 0.4572116 -1.0041098
18855_3_U 2 18157918 0.5909283 -1.1455295
Sample_ID Individual Sex Replicate treatment RIN
18855_3_S 18855_3_S NA18855 F 3 Unstrain 10.0
19160_3_S 19160_3_S NA19160 M 3 Strain 10.0
18856_3_U 18856_3_U NA18856 M 3 Unstrain 10.0
18856_1_U 18856_1_U NA18856 M 1 Unstrain 9.9
18855_2_S 18855_2_S NA18855 F 2 Unstrain 10.0
18856_2_S 18856_2_S NA18856 M 2 Strain 9.6
19160_3_U 19160_3_U NA19160 M 3 Strain 10.0
18855_2_U 18855_2_U NA18855 F 2 Unstrain 10.0
19160_2_S 19160_2_S NA19160 M 2 Strain 10.0
18855_1_S 18855_1_S NA18855 F 1 Unstrain 9.9
18856_1_S 18856_1_S NA18856 M 1 Strain 8.5
19160_1_S 19160_1_S NA19160 M 1 Strain 9.9
19160_2_U 19160_2_U NA19160 M 2 Strain 10.0
18855_1_U 18855_1_U NA18855 F 1 Unstrain 10.0
18856_3_S 18856_3_S NA18856 M 3 Strain 9.6
18856_2_U 18856_2_U NA18856 M 2 Unstrain 9.5
18855_3_U 18855_3_U NA18855 F 3 Strain 10.0
LibraryPrepBatch LibSize W_1 W_2
18855_3_S 2 15419569 0.6403178 -1.0334196
19160_3_S 2 15177653 0.4887528 -1.3355831
18856_3_U 2 13722909 0.9502388 -0.7892404
18856_1_U 1 18647599 1.2228123 -1.4496449
18855_2_S 1 18256163 0.8233400 -0.6748150
18856_2_S 1 16782764 0.0515988 -1.2784836
19160_3_U 2 19839604 0.3406102 -1.4859733
18855_2_U 1 18618691 0.7609177 -0.8596946
19160_2_S 1 16622407 0.4247172 -1.4414851
18855_1_S 1 19712744 0.7356719 -1.4499412
18856_1_S 1 13571439 1.1409773 -1.1686123
19160_1_S 1 20979958 0.5909682 -1.9538068
19160_2_U 1 20924281 0.1295380 -1.8537818
18855_1_U 1 20146697 0.7569979 -1.3228202
18856_3_S 2 15807863 0.8205467 -0.8981659
18856_2_U 1 15142152 0.4572116 -1.0041098
18855_3_U 2 18157918 0.5909283 -1.1455295
for (permutation in 7:12){
results <- permuted_results[[permutation]]
# k-s test making use of ranked based on p-values
genes <- results$qvalue
names(genes) <- rownames(results)
selection <- function(allScore){ return(allScore < 0.05)} # function that returns TRUE/FALSE for p-values<0.05
allGO2genes <- annFUN.org(whichOnto="BP", feasibleGenes=NULL, mapping="org.Hs.eg.db", ID="ensembl")
GOdata <- new("topGOdata",
ontology="BP",
allGenes=genes,
annot=annFUN.GO2genes,
GO2genes=allGO2genes,
geneSel=selection,
nodeSize=10)
results.ks <- runTest(GOdata, algorithm="classic", statistic="ks")
goEnrichment <- GenTable(GOdata, KS=results.ks, orderBy="KS", topNodes=20)
goEnrichment <- goEnrichment %>%
mutate(KS = ifelse(grepl("<", KS), 1e-30, KS))
goEnrichment$KS <- as.numeric(goEnrichment$KS)
goEnrichment <- goEnrichment[goEnrichment$KS<0.05,]
goEnrichment <- goEnrichment[,c("GO.ID","Term","KS")]
goEnrichment$Term <- gsub(" [a-z]*\\.\\.\\.$", "", goEnrichment$Term)
goEnrichment$Term <- gsub("\\.\\.\\.$", "", goEnrichment$Term)
goEnrichment$Term <- paste(goEnrichment$GO.ID, goEnrichment$Term, sep=", ")
goEnrichment$Term <- factor(goEnrichment$Term, levels=rev(goEnrichment$Term))
goEnrichment$KS <- as.numeric(goEnrichment$KS)
plot <- ggplot(goEnrichment, aes(x=Term, y=-log10(KS))) +
stat_summary(geom = "bar", fun = mean, position = "dodge") +
xlab("Biological process") +
ylab("Enrichment") +
ggtitle("Title") +
scale_y_continuous(breaks = round(seq(0, max(-log10(goEnrichment$KS)), by = 2), 1)) +
theme_bw(base_size=24) +
theme(
legend.position='none',
legend.background=element_rect(),
plot.title=element_text(angle=0, size=24, face="bold", vjust=1),
axis.text.x=element_text(angle=0, size=18, face="bold", hjust=1.10),
axis.text.y=element_text(angle=0, size=18, face="bold", vjust=0.5),
axis.title=element_text(size=24, face="bold"),
legend.key=element_blank(), #removes the border
legend.key.size=unit(1, "cm"), #Sets overall area/size of the legend
legend.text=element_text(size=18), #Text size
title=element_text(size=18)) +
guides(colour=guide_legend(override.aes=list(size=2.5))) +
coord_flip()
print(plot)
}
Building most specific GOs .....
( 9770 GO terms found. )
Build GO DAG topology ..........
( 13850 GO terms and 32624 relations. )
Annotating nodes ...............
( 9172 genes annotated to the GO terms. )
-- Classic Algorithm --
the algorithm is scoring 5173 nontrivial nodes
parameters:
test statistic: ks
score order: increasing
Building most specific GOs .....
( 9770 GO terms found. )
Build GO DAG topology ..........
( 13850 GO terms and 32624 relations. )
Annotating nodes ...............
( 9172 genes annotated to the GO terms. )
-- Classic Algorithm --
the algorithm is scoring 5173 nontrivial nodes
parameters:
test statistic: ks
score order: increasing

| Version | Author | Date |
|---|---|---|
| 6d6d1b5 | Anthony Hung | 2020-10-04 |
Building most specific GOs .....
( 9770 GO terms found. )
Build GO DAG topology ..........
( 13850 GO terms and 32624 relations. )
Annotating nodes ...............
( 9172 genes annotated to the GO terms. )
-- Classic Algorithm --
the algorithm is scoring 5173 nontrivial nodes
parameters:
test statistic: ks
score order: increasing

| Version | Author | Date |
|---|---|---|
| 6d6d1b5 | Anthony Hung | 2020-10-04 |
Building most specific GOs .....
( 9770 GO terms found. )
Build GO DAG topology ..........
( 13850 GO terms and 32624 relations. )
Annotating nodes ...............
( 9172 genes annotated to the GO terms. )
-- Classic Algorithm --
the algorithm is scoring 5173 nontrivial nodes
parameters:
test statistic: ks
score order: increasing

| Version | Author | Date |
|---|---|---|
| 6d6d1b5 | Anthony Hung | 2020-10-04 |
Building most specific GOs .....
( 9770 GO terms found. )
Build GO DAG topology ..........
( 13850 GO terms and 32624 relations. )
Annotating nodes ...............
( 9172 genes annotated to the GO terms. )
-- Classic Algorithm --
the algorithm is scoring 5173 nontrivial nodes
parameters:
test statistic: ks
score order: increasing

| Version | Author | Date |
|---|---|---|
| 6d6d1b5 | Anthony Hung | 2020-10-04 |
Building most specific GOs .....
( 9770 GO terms found. )
Build GO DAG topology ..........
( 13850 GO terms and 32624 relations. )
Annotating nodes ...............
( 9172 genes annotated to the GO terms. )
-- Classic Algorithm --
the algorithm is scoring 5173 nontrivial nodes
parameters:
test statistic: ks
score order: increasing

| Version | Author | Date |
|---|---|---|
| 6d6d1b5 | Anthony Hung | 2020-10-04 |

| Version | Author | Date |
|---|---|---|
| 6d6d1b5 | Anthony Hung | 2020-10-04 |
for (permutation in 7:12){
results <- permuted_results[[permutation]]
# k-s test making use of ranked based on p-values
genes <- results$qvalue
names(genes) <- rownames(results)
selection <- function(allScore){ return(allScore < 0.05)} # function that returns TRUE/FALSE for p-values<0.05
allGO2genes <- annFUN.org(whichOnto="BP", feasibleGenes=NULL, mapping="org.Hs.eg.db", ID="ensembl")
GOdata <- new("topGOdata",
ontology="BP",
allGenes=genes,
annot=annFUN.GO2genes,
GO2genes=allGO2genes,
geneSel=selection,
nodeSize=10)
results.ks <- runTest(GOdata, algorithm="classic", statistic="ks")
goEnrichment <- GenTable(GOdata, KS=results.ks, orderBy="KS", topNodes=20)
goEnrichment <- goEnrichment %>%
mutate(KS = ifelse(grepl("<", KS), 1e-30, KS))
goEnrichment$KS <- as.numeric(goEnrichment$KS)
goEnrichment <- goEnrichment[goEnrichment$KS<0.05,]
goEnrichment <- goEnrichment[,c("GO.ID","Term","KS")]
goEnrichment$Term <- gsub(" [a-z]*\\.\\.\\.$", "", goEnrichment$Term)
goEnrichment$Term <- gsub("\\.\\.\\.$", "", goEnrichment$Term)
goEnrichment$Term <- paste(goEnrichment$GO.ID, goEnrichment$Term, sep=", ")
goEnrichment$Term <- factor(goEnrichment$Term, levels=rev(goEnrichment$Term))
goEnrichment$KS <- as.numeric(goEnrichment$KS)
print(goEnrichment$Term)
}
Building most specific GOs .....
( 9770 GO terms found. )
Build GO DAG topology ..........
( 13850 GO terms and 32624 relations. )
Annotating nodes ...............
( 9172 genes annotated to the GO terms. )
-- Classic Algorithm --
the algorithm is scoring 5173 nontrivial nodes
parameters:
test statistic: ks
score order: increasing
[1] GO:0010469, regulation of signaling receptor
[2] GO:0048729, tissue morphogenesis
[3] GO:0051270, regulation of cellular component
[4] GO:0007186, G protein-coupled receptor signaling
[5] GO:0009653, anatomical structure morphogenesis
[6] GO:0006928, movement of cell or subcellular
[7] GO:0035107, appendage morphogenesis
[8] GO:0035108, limb morphogenesis
[9] GO:0002009, morphogenesis of an epithelium
[10] GO:0042330, taxis
[11] GO:0040011, locomotion
[12] GO:0035295, tube development
[13] GO:0042035, regulation of cytokine biosynthetic
[14] GO:0006935, chemotaxis
[15] GO:0060541, respiratory system development
[16] GO:0050819, negative regulation of coagulation
[17] GO:0060562, epithelial tube morphogenesis
[18] GO:0032501, multicellular organismal process
[19] GO:0150076, neuroinflammatory response
[20] GO:0048646, anatomical structure formation involved
20 Levels: GO:0048646, anatomical structure formation involved ...
Building most specific GOs .....
( 9770 GO terms found. )
Build GO DAG topology ..........
( 13850 GO terms and 32624 relations. )
Annotating nodes ...............
( 9172 genes annotated to the GO terms. )
-- Classic Algorithm --
the algorithm is scoring 5173 nontrivial nodes
parameters:
test statistic: ks
score order: increasing
[1] GO:0010591, regulation of lamellipodium assembly
[2] GO:0043038, amino acid activation
[3] GO:0043039, tRNA aminoacylation
[4] GO:0003230, cardiac atrium development
[5] GO:0006418, tRNA aminoacylation for protein
[6] GO:0010592, positive regulation of lamellipodium
[7] GO:0032635, interleukin-6 production
[8] GO:0043489, RNA stabilization
[9] GO:0021536, diencephalon development
[10] GO:0003209, cardiac atrium morphogenesis
[11] GO:0003283, atrial septum development
[12] GO:0006928, movement of cell or subcellular
[13] GO:0060021, roof of mouth development
[14] GO:0050919, negative chemotaxis
[15] GO:0048255, mRNA stabilization
[16] GO:0032781, positive regulation of ATPase activity
[17] GO:0045669, positive regulation of osteoblast
[18] GO:0061029, eyelid development in camera-type eye
[19] GO:0043486, histone exchange
[20] GO:0007156, homophilic cell adhesion via plasma
20 Levels: GO:0007156, homophilic cell adhesion via plasma ...
Building most specific GOs .....
( 9770 GO terms found. )
Build GO DAG topology ..........
( 13850 GO terms and 32624 relations. )
Annotating nodes ...............
( 9172 genes annotated to the GO terms. )
-- Classic Algorithm --
the algorithm is scoring 5173 nontrivial nodes
parameters:
test statistic: ks
score order: increasing
[1] GO:0010591, regulation of lamellipodium assembly
[2] GO:0043038, amino acid activation
[3] GO:0043039, tRNA aminoacylation
[4] GO:0003230, cardiac atrium development
[5] GO:0006418, tRNA aminoacylation for protein
[6] GO:0010592, positive regulation of lamellipodium
[7] GO:0032635, interleukin-6 production
[8] GO:0043489, RNA stabilization
[9] GO:0021536, diencephalon development
[10] GO:0003209, cardiac atrium morphogenesis
[11] GO:0003283, atrial septum development
[12] GO:0006928, movement of cell or subcellular
[13] GO:0060021, roof of mouth development
[14] GO:0050919, negative chemotaxis
[15] GO:0048255, mRNA stabilization
[16] GO:0032781, positive regulation of ATPase activity
[17] GO:0045669, positive regulation of osteoblast
[18] GO:0061029, eyelid development in camera-type eye
[19] GO:0043486, histone exchange
[20] GO:0007156, homophilic cell adhesion via plasma
20 Levels: GO:0007156, homophilic cell adhesion via plasma ...
Building most specific GOs .....
( 9770 GO terms found. )
Build GO DAG topology ..........
( 13850 GO terms and 32624 relations. )
Annotating nodes ...............
( 9172 genes annotated to the GO terms. )
-- Classic Algorithm --
the algorithm is scoring 5173 nontrivial nodes
parameters:
test statistic: ks
score order: increasing
[1] GO:0010591, regulation of lamellipodium assembly
[2] GO:0043038, amino acid activation
[3] GO:0043039, tRNA aminoacylation
[4] GO:0003230, cardiac atrium development
[5] GO:0006418, tRNA aminoacylation for protein
[6] GO:0010592, positive regulation of lamellipodium
[7] GO:0032635, interleukin-6 production
[8] GO:0043489, RNA stabilization
[9] GO:0021536, diencephalon development
[10] GO:0003209, cardiac atrium morphogenesis
[11] GO:0003283, atrial septum development
[12] GO:0006928, movement of cell or subcellular
[13] GO:0060021, roof of mouth development
[14] GO:0050919, negative chemotaxis
[15] GO:0048255, mRNA stabilization
[16] GO:0032781, positive regulation of ATPase activity
[17] GO:0045669, positive regulation of osteoblast
[18] GO:0061029, eyelid development in camera-type eye
[19] GO:0043486, histone exchange
[20] GO:0007156, homophilic cell adhesion via plasma
20 Levels: GO:0007156, homophilic cell adhesion via plasma ...
Building most specific GOs .....
( 9770 GO terms found. )
Build GO DAG topology ..........
( 13850 GO terms and 32624 relations. )
Annotating nodes ...............
( 9172 genes annotated to the GO terms. )
-- Classic Algorithm --
the algorithm is scoring 5173 nontrivial nodes
parameters:
test statistic: ks
score order: increasing
[1] GO:0010469, regulation of signaling receptor
[2] GO:0007186, G protein-coupled receptor signaling
[3] GO:0072359, circulatory system development
[4] GO:0048568, embryonic organ development
[5] GO:0030198, extracellular matrix organization
[6] GO:0072001, renal system development
[7] GO:0003008, system process
[8] GO:0001944, vasculature development
[9] GO:0001655, urogenital system development
[10] GO:0006935, chemotaxis
[11] GO:0043062, extracellular structure organization
[12] GO:0001822, kidney development
[13] GO:0042330, taxis
[14] GO:0040011, locomotion
[15] GO:0035295, tube development
[16] GO:0072358, cardiovascular system development
[17] GO:0072006, nephron development
[18] GO:0035239, tube morphogenesis
[19] GO:0032501, multicellular organismal process
[20] GO:0001568, blood vessel development
20 Levels: GO:0001568, blood vessel development ...
Building most specific GOs .....
( 9770 GO terms found. )
Build GO DAG topology ..........
( 13850 GO terms and 32624 relations. )
Annotating nodes ...............
( 9172 genes annotated to the GO terms. )
-- Classic Algorithm --
the algorithm is scoring 5173 nontrivial nodes
parameters:
test statistic: ks
score order: increasing
[1] GO:0035295, tube development
[2] GO:0072359, circulatory system development
[3] GO:0009653, anatomical structure morphogenesis
[4] GO:0035239, tube morphogenesis
[5] GO:0032502, developmental process
[6] GO:0008283, cell proliferation
[7] GO:0023052, signaling
[8] GO:0001944, vasculature development
[9] GO:0007275, multicellular organism development
[10] GO:0048856, anatomical structure development
[11] GO:0048646, anatomical structure formation involved
[12] GO:0030334, regulation of cell migration
[13] GO:0051270, regulation of cellular component
[14] GO:0072358, cardiovascular system development
[15] GO:0001568, blood vessel development
[16] GO:0007154, cell communication
[17] GO:0040012, regulation of locomotion
[18] GO:0042127, regulation of cell proliferation
[19] GO:0008015, blood circulation
[20] GO:0016477, cell migration
20 Levels: GO:0016477, cell migration ... GO:0035295, tube development
sessionInfo()
R version 3.6.1 (2019-07-05)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Scientific Linux 7.4 (Nitrogen)
Matrix products: default
BLAS/LAPACK: /software/openblas-0.2.19-el7-x86_64/lib/libopenblas_haswellp-r0.2.19.so
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats4 parallel grid stats graphics grDevices utils
[8] datasets methods base
other attached packages:
[1] org.Hs.eg.db_3.8.2 topGO_2.36.0
[3] SparseM_1.78 GO.db_3.8.2
[5] AnnotationDbi_1.46.0 graph_1.62.0
[7] RUVSeq_1.18.0 EDASeq_2.18.0
[9] ShortRead_1.42.0 GenomicAlignments_1.20.1
[11] SummarizedExperiment_1.14.1 DelayedArray_0.10.0
[13] matrixStats_0.57.0 Rsamtools_2.0.0
[15] GenomicRanges_1.36.1 GenomeInfoDb_1.20.0
[17] Biostrings_2.52.0 XVector_0.24.0
[19] IRanges_2.18.3 S4Vectors_0.22.1
[21] BiocParallel_1.18.1 Biobase_2.44.0
[23] BiocGenerics_0.30.0 VennDiagram_1.6.20
[25] futile.logger_1.4.3 cowplot_1.1.0
[27] ggplot2_3.3.2 ashr_2.2-47
[29] tidyr_1.1.2 dplyr_1.0.2
[31] edgeR_3.26.5 plyr_1.8.6
[33] limma_3.40.6
loaded via a namespace (and not attached):
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[4] rprojroot_1.3-2 htmlTable_1.13.1 base64enc_0.1-3
[7] fs_1.3.1 rstudioapi_0.11 farver_2.0.3
[10] bit64_0.9-7 splines_3.6.1 R.methodsS3_1.8.1
[13] DESeq_1.36.0 geneplotter_1.62.0 knitr_1.23
[16] Formula_1.2-3 workflowr_1.6.2 annotate_1.62.0
[19] cluster_2.1.0 R.oo_1.24.0 compiler_3.6.1
[22] httr_1.4.2 backports_1.1.10 Matrix_1.2-18
[25] later_1.1.0.1 formatR_1.7 acepack_1.4.1
[28] htmltools_0.5.0 prettyunits_1.1.1 tools_3.6.1
[31] gtable_0.3.0 glue_1.4.2 GenomeInfoDbData_1.2.1
[34] Rcpp_1.0.5 vctrs_0.3.4 rtracklayer_1.44.0
[37] xfun_0.8 stringr_1.4.0 lifecycle_0.2.0
[40] irlba_2.3.3 statmod_1.4.32 XML_3.98-1.20
[43] zlibbioc_1.30.0 MASS_7.3-52 scales_1.1.1
[46] aroma.light_3.14.0 hms_0.5.3 promises_1.1.1
[49] lambda.r_1.2.4 RColorBrewer_1.1-2 yaml_2.2.1
[52] gridExtra_2.3 memoise_1.1.0 rpart_4.1-15
[55] biomaRt_2.40.1 latticeExtra_0.6-28 stringi_1.4.6
[58] RSQLite_2.1.1 SQUAREM_2020.4 genefilter_1.66.0
[61] checkmate_1.9.4 GenomicFeatures_1.36.3 truncnorm_1.0-8
[64] rlang_0.4.8 pkgconfig_2.0.3 bitops_1.0-6
[67] evaluate_0.14 lattice_0.20-41 invgamma_1.1
[70] purrr_0.3.4 htmlwidgets_1.5.2 labeling_0.3
[73] bit_4.0.4 tidyselect_1.1.0 magrittr_1.5
[76] R6_2.4.1 Hmisc_4.2-0 generics_0.0.2
[79] DBI_1.1.0 foreign_0.8-71 pillar_1.4.6
[82] whisker_0.3-2 withr_2.3.0 nnet_7.3-12
[85] survival_2.44-1.1 RCurl_1.98-1.1 mixsqp_0.3-43
[88] tibble_3.0.4 crayon_1.3.4 futile.options_1.0.1
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[94] data.table_1.13.0 blob_1.2.0 git2r_0.26.1
[97] etrunct_0.1 digest_0.6.25 xtable_1.8-4
[100] httpuv_1.5.1 R.utils_2.9.0 munsell_0.5.0